Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark
The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two para...
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description | The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two parasites by immunofluorescence microscopy. A large proportion of the (oo)cyst containing samples were selected for molecular characterization. Sequencing and phylogenetic analysis of the 18S rDNA locus and/or the HSP70 gene of 183 pig and 154 cattle isolates of Cryptosporidium revealed the presence of C. suis, pig genotype II, C. parvum (cattle genotype), C. bovis, Cryptosporidium deer-like genotype and a novel C. suis-like genotype. For both cattle and pigs, a host age-related change in distribution of species/genotypes was observed. The zoonotic C. parvum (cattle genotype) was most prevalent in young calves. For Giardia, 82 and 145 isolates from pigs and cattle, respectively, were analysed at the 18S rDNA locus and/or the gdh gene. Giardia isolates belonging to the zoonotic Assemblage A was found in both young and older calves, as well as in weaners and piglets, whereas cows seemed to be infected purely by isolates of the livestock group, Assemblage E. |
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B. ; VIGRE, H. ; ENEMARK, H. L. ; MADDOX-HYTTEL, C.</creator><creatorcontrib>LANGKJÆR, R. B. ; VIGRE, H. ; ENEMARK, H. L. ; MADDOX-HYTTEL, C.</creatorcontrib><description>The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two parasites by immunofluorescence microscopy. A large proportion of the (oo)cyst containing samples were selected for molecular characterization. Sequencing and phylogenetic analysis of the 18S rDNA locus and/or the HSP70 gene of 183 pig and 154 cattle isolates of Cryptosporidium revealed the presence of C. suis, pig genotype II, C. parvum (cattle genotype), C. bovis, Cryptosporidium deer-like genotype and a novel C. suis-like genotype. For both cattle and pigs, a host age-related change in distribution of species/genotypes was observed. The zoonotic C. parvum (cattle genotype) was most prevalent in young calves. For Giardia, 82 and 145 isolates from pigs and cattle, respectively, were analysed at the 18S rDNA locus and/or the gdh gene. Giardia isolates belonging to the zoonotic Assemblage A was found in both young and older calves, as well as in weaners and piglets, whereas cows seemed to be infected purely by isolates of the livestock group, Assemblage E.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182006001533</identifier><identifier>PMID: 17076923</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Age Distribution ; animal age ; Animals ; Biological and medical sciences ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - parasitology ; Cryptosporidiosis - epidemiology ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - veterinary ; Cryptosporidium ; Cryptosporidium - classification ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium spp ; Cysts ; Dairy cattle ; Denmark ; Denmark - epidemiology ; DNA, Protozoan - analysis ; DNA, Ribosomal - analysis ; Female ; Fundamental and applied biological sciences. Psychology ; gdh gene ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; genes ; Genetic diversity ; Genetic Variation ; Genotype ; Genotypes ; Giardia - classification ; Giardia - genetics ; Giardia - isolation & purification ; Giardia duodenalis ; Giardia lamblia ; Giardiasis - epidemiology ; Giardiasis - parasitology ; Giardiasis - veterinary ; heat shock protein 70 gene ; Hogs ; host age ; host strains ; Invertebrates ; Livestock ; Male ; Microbiology ; Molecular Sequence Data ; nucleotide sequences ; Parasites ; Phylogeny ; pig ; Prevalence ; Protozoan Proteins - genetics ; ribosomal DNA ; Sequence Analysis, DNA ; sequence homology ; Studies ; Swine ; Swine Diseases - epidemiology ; Swine Diseases - parasitology ; Zoonoses ; zoonotic genotypes</subject><ispartof>Parasitology, 2007-03, Vol.134 (3), p.339-350</ispartof><rights>2006 Cambridge University Press</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Cambridge University Press Mar 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-acd2ceb6d0ac76e63f1e3d551e8c8f962f993fd505d3cbc4b4014f300ce560d63</citedby><cites>FETCH-LOGICAL-c528t-acd2ceb6d0ac76e63f1e3d551e8c8f962f993fd505d3cbc4b4014f300ce560d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182006001533/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,777,781,27905,27906,55609</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18497549$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17076923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LANGKJÆR, R. B.</creatorcontrib><creatorcontrib>VIGRE, H.</creatorcontrib><creatorcontrib>ENEMARK, H. L.</creatorcontrib><creatorcontrib>MADDOX-HYTTEL, C.</creatorcontrib><title>Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two parasites by immunofluorescence microscopy. A large proportion of the (oo)cyst containing samples were selected for molecular characterization. Sequencing and phylogenetic analysis of the 18S rDNA locus and/or the HSP70 gene of 183 pig and 154 cattle isolates of Cryptosporidium revealed the presence of C. suis, pig genotype II, C. parvum (cattle genotype), C. bovis, Cryptosporidium deer-like genotype and a novel C. suis-like genotype. For both cattle and pigs, a host age-related change in distribution of species/genotypes was observed. The zoonotic C. parvum (cattle genotype) was most prevalent in young calves. For Giardia, 82 and 145 isolates from pigs and cattle, respectively, were analysed at the 18S rDNA locus and/or the gdh gene. Giardia isolates belonging to the zoonotic Assemblage A was found in both young and older calves, as well as in weaners and piglets, whereas cows seemed to be infected purely by isolates of the livestock group, Assemblage E.</description><subject>Age Distribution</subject><subject>animal age</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - parasitology</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium spp</subject><subject>Cysts</subject><subject>Dairy cattle</subject><subject>Denmark</subject><subject>Denmark - epidemiology</subject><subject>DNA, Protozoan - analysis</subject><subject>DNA, Ribosomal - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gdh gene</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>genes</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Giardia - classification</subject><subject>Giardia - genetics</subject><subject>Giardia - isolation & purification</subject><subject>Giardia duodenalis</subject><subject>Giardia lamblia</subject><subject>Giardiasis - epidemiology</subject><subject>Giardiasis - parasitology</subject><subject>Giardiasis - veterinary</subject><subject>heat shock protein 70 gene</subject><subject>Hogs</subject><subject>host age</subject><subject>host strains</subject><subject>Invertebrates</subject><subject>Livestock</subject><subject>Male</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Parasites</subject><subject>Phylogeny</subject><subject>pig</subject><subject>Prevalence</subject><subject>Protozoan Proteins - genetics</subject><subject>ribosomal DNA</subject><subject>Sequence Analysis, DNA</subject><subject>sequence homology</subject><subject>Studies</subject><subject>Swine</subject><subject>Swine Diseases - epidemiology</subject><subject>Swine Diseases - parasitology</subject><subject>Zoonoses</subject><subject>zoonotic genotypes</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kc1u1DAUhS0EotPCA7CBCAl2gWs7dpJlNQMDqAhVbVl0Y3n8M_WQxMFOpA5Pj9OJGAnEypLvd459zkXoBYZ3GHD5_gqAYlwRAA6AGaWP0AIXvM4rzPFjtJjG-TQ_Qacx7iBxlJOn6ASXUPKa0AXaffWNUWMjQyY7nfV3-8ZvTWcGpzJ1J4NUgwnulxyc7zJvs2XY94OPvQ9Ou7F9EK2dDNrJzAbfZr3bxodbJYehMZnrspXpWhl-PENPrGyieT6fZ-jm44fr5af84tv68_L8IleMVEMulSbKbLgGqUpuOLXYUM0YNpWqbM2JrWtqNQOmqdqoYlMALiwFUIZx0JyeobcH3z74n6OJg2hdVKZpZGf8GAWvap5sqgS-_gvc-TF06W-CpBoJcDxB-ACp4GMMxoo-uBRnLzCIaQviny0kzcvZeNy0Rh8Vc-0JeDMDMirZ2CA75eKRq4q6ZEWduPzAuTiY-z_zVKbgJS2Z4OtLcXu7Wl1-X30R14l_deCt9EJuQ_K8uSKAUzslIyVML9M5jmw3aYdbcwz9_0C_AUV3t3s</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>LANGKJÆR, R. 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B. ; VIGRE, H. ; ENEMARK, H. L. ; MADDOX-HYTTEL, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-acd2ceb6d0ac76e63f1e3d551e8c8f962f993fd505d3cbc4b4014f300ce560d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Age Distribution</topic><topic>animal age</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - parasitology</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - classification</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium spp</topic><topic>Cysts</topic><topic>Dairy cattle</topic><topic>Denmark</topic><topic>Denmark - epidemiology</topic><topic>DNA, Protozoan - analysis</topic><topic>DNA, Ribosomal - analysis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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Models</topic><topic>genes</topic><topic>Genetic diversity</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Giardia - classification</topic><topic>Giardia - genetics</topic><topic>Giardia - isolation & purification</topic><topic>Giardia duodenalis</topic><topic>Giardia lamblia</topic><topic>Giardiasis - epidemiology</topic><topic>Giardiasis - parasitology</topic><topic>Giardiasis - veterinary</topic><topic>heat shock protein 70 gene</topic><topic>Hogs</topic><topic>host age</topic><topic>host strains</topic><topic>Invertebrates</topic><topic>Livestock</topic><topic>Male</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Parasites</topic><topic>Phylogeny</topic><topic>pig</topic><topic>Prevalence</topic><topic>Protozoan Proteins - genetics</topic><topic>ribosomal DNA</topic><topic>Sequence Analysis, DNA</topic><topic>sequence homology</topic><topic>Studies</topic><topic>Swine</topic><topic>Swine Diseases - epidemiology</topic><topic>Swine Diseases - parasitology</topic><topic>Zoonoses</topic><topic>zoonotic genotypes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LANGKJÆR, R. 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B.</au><au>VIGRE, H.</au><au>ENEMARK, H. L.</au><au>MADDOX-HYTTEL, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>134</volume><issue>3</issue><spage>339</spage><epage>350</epage><pages>339-350</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two parasites by immunofluorescence microscopy. A large proportion of the (oo)cyst containing samples were selected for molecular characterization. Sequencing and phylogenetic analysis of the 18S rDNA locus and/or the HSP70 gene of 183 pig and 154 cattle isolates of Cryptosporidium revealed the presence of C. suis, pig genotype II, C. parvum (cattle genotype), C. bovis, Cryptosporidium deer-like genotype and a novel C. suis-like genotype. For both cattle and pigs, a host age-related change in distribution of species/genotypes was observed. The zoonotic C. parvum (cattle genotype) was most prevalent in young calves. For Giardia, 82 and 145 isolates from pigs and cattle, respectively, were analysed at the 18S rDNA locus and/or the gdh gene. Giardia isolates belonging to the zoonotic Assemblage A was found in both young and older calves, as well as in weaners and piglets, whereas cows seemed to be infected purely by isolates of the livestock group, Assemblage E.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>17076923</pmid><doi>10.1017/S0031182006001533</doi><tpages>12</tpages></addata></record> |
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subjects | Age Distribution animal age Animals Biological and medical sciences Cattle Cattle Diseases - epidemiology Cattle Diseases - parasitology Cryptosporidiosis - epidemiology Cryptosporidiosis - parasitology Cryptosporidiosis - veterinary Cryptosporidium Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium spp Cysts Dairy cattle Denmark Denmark - epidemiology DNA, Protozoan - analysis DNA, Ribosomal - analysis Female Fundamental and applied biological sciences. Psychology gdh gene General aspects General aspects and techniques. Study of several systematic groups. Models genes Genetic diversity Genetic Variation Genotype Genotypes Giardia - classification Giardia - genetics Giardia - isolation & purification Giardia duodenalis Giardia lamblia Giardiasis - epidemiology Giardiasis - parasitology Giardiasis - veterinary heat shock protein 70 gene Hogs host age host strains Invertebrates Livestock Male Microbiology Molecular Sequence Data nucleotide sequences Parasites Phylogeny pig Prevalence Protozoan Proteins - genetics ribosomal DNA Sequence Analysis, DNA sequence homology Studies Swine Swine Diseases - epidemiology Swine Diseases - parasitology Zoonoses zoonotic genotypes |
title | Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark |
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