Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark

The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two para...

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Veröffentlicht in:Parasitology 2007-03, Vol.134 (3), p.339-350
Hauptverfasser: LANGKJÆR, R. B., VIGRE, H., ENEMARK, H. L., MADDOX-HYTTEL, C.
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VIGRE, H.
ENEMARK, H. L.
MADDOX-HYTTEL, C.
description The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two parasites by immunofluorescence microscopy. A large proportion of the (oo)cyst containing samples were selected for molecular characterization. Sequencing and phylogenetic analysis of the 18S rDNA locus and/or the HSP70 gene of 183 pig and 154 cattle isolates of Cryptosporidium revealed the presence of C. suis, pig genotype II, C. parvum (cattle genotype), C. bovis, Cryptosporidium deer-like genotype and a novel C. suis-like genotype. For both cattle and pigs, a host age-related change in distribution of species/genotypes was observed. The zoonotic C. parvum (cattle genotype) was most prevalent in young calves. For Giardia, 82 and 145 isolates from pigs and cattle, respectively, were analysed at the 18S rDNA locus and/or the gdh gene. Giardia isolates belonging to the zoonotic Assemblage A was found in both young and older calves, as well as in weaners and piglets, whereas cows seemed to be infected purely by isolates of the livestock group, Assemblage E.
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For both cattle and pigs, a host age-related change in distribution of species/genotypes was observed. The zoonotic C. parvum (cattle genotype) was most prevalent in young calves. For Giardia, 82 and 145 isolates from pigs and cattle, respectively, were analysed at the 18S rDNA locus and/or the gdh gene. 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B.</creatorcontrib><creatorcontrib>VIGRE, H.</creatorcontrib><creatorcontrib>ENEMARK, H. L.</creatorcontrib><creatorcontrib>MADDOX-HYTTEL, C.</creatorcontrib><title>Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two parasites by immunofluorescence microscopy. A large proportion of the (oo)cyst containing samples were selected for molecular characterization. Sequencing and phylogenetic analysis of the 18S rDNA locus and/or the HSP70 gene of 183 pig and 154 cattle isolates of Cryptosporidium revealed the presence of C. suis, pig genotype II, C. parvum (cattle genotype), C. bovis, Cryptosporidium deer-like genotype and a novel C. suis-like genotype. For both cattle and pigs, a host age-related change in distribution of species/genotypes was observed. The zoonotic C. parvum (cattle genotype) was most prevalent in young calves. For Giardia, 82 and 145 isolates from pigs and cattle, respectively, were analysed at the 18S rDNA locus and/or the gdh gene. 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B.</au><au>VIGRE, H.</au><au>ENEMARK, H. L.</au><au>MADDOX-HYTTEL, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>134</volume><issue>3</issue><spage>339</spage><epage>350</epage><pages>339-350</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>The genetic diversity of Cryptosporidium spp. and Giardia duodenalis from dairy cattle and pigs in Denmark was determined in the present study. Faecal samples from 1237 pigs and 1150 cattle originating from 50 sow herds and 50 dairy herds, respectively, were analysed for the presence of the two parasites by immunofluorescence microscopy. A large proportion of the (oo)cyst containing samples were selected for molecular characterization. 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subjects Age Distribution
animal age
Animals
Biological and medical sciences
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - parasitology
Cryptosporidiosis - epidemiology
Cryptosporidiosis - parasitology
Cryptosporidiosis - veterinary
Cryptosporidium
Cryptosporidium - classification
Cryptosporidium - genetics
Cryptosporidium - isolation & purification
Cryptosporidium spp
Cysts
Dairy cattle
Denmark
Denmark - epidemiology
DNA, Protozoan - analysis
DNA, Ribosomal - analysis
Female
Fundamental and applied biological sciences. Psychology
gdh gene
General aspects
General aspects and techniques. Study of several systematic groups. Models
genes
Genetic diversity
Genetic Variation
Genotype
Genotypes
Giardia - classification
Giardia - genetics
Giardia - isolation & purification
Giardia duodenalis
Giardia lamblia
Giardiasis - epidemiology
Giardiasis - parasitology
Giardiasis - veterinary
heat shock protein 70 gene
Hogs
host age
host strains
Invertebrates
Livestock
Male
Microbiology
Molecular Sequence Data
nucleotide sequences
Parasites
Phylogeny
pig
Prevalence
Protozoan Proteins - genetics
ribosomal DNA
Sequence Analysis, DNA
sequence homology
Studies
Swine
Swine Diseases - epidemiology
Swine Diseases - parasitology
Zoonoses
zoonotic genotypes
title Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark
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