Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry
We examined hydrogen production from a dairy cow waste slurry (13.4 g of volatile solids per liter) by batch cultures in a temperature range from 37 to 85°C, using microflora naturally present within the slurry. Without the addition of seed bacteria, hydrogen was produced by simply incubating the sl...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2007-02, Vol.74 (2), p.474-483 |
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| creator | Yokoyama, Hiroshi Waki, Miyoko Moriya, Naoko Yasuda, Tomoko Tanaka, Yasuo Haga, Kiyonori |
| description | We examined hydrogen production from a dairy cow waste slurry (13.4 g of volatile solids per liter) by batch cultures in a temperature range from 37 to 85°C, using microflora naturally present within the slurry. Without the addition of seed bacteria, hydrogen was produced by simply incubating the slurry, using the microflora within the slurry. Interestingly, two peaks of fermentation temperatures for hydrogen production from the slurry were observed at 60 and 75°C (392 and 248 ml H₂ per liter of slurry, respectively). After the termination of the hydrogen evolution, the microflora cultured at 60°C displayed hydrogen-consuming activity, but hydrogen-consuming activity of the microflora cultured at 75°C was not detected, at least for 24 days. At both 60 and 75°C, the main by-product was acetate, and the optimum pH of the slurry for hydrogen production was around neutral. Bacteria related to hydrogen-producing moderate and extreme thermophiles, Clostridium thermocellum and Caldanaerobacter subterraneus, were detected in the slurries cultured at 60 and 75°C, respectively, by denaturing gradient gel electrophoresis analyses, using the V3 region of 16S rDNA. |
| doi_str_mv | 10.1007/s00253-006-0647-4 |
| format | Article |
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Without the addition of seed bacteria, hydrogen was produced by simply incubating the slurry, using the microflora within the slurry. Interestingly, two peaks of fermentation temperatures for hydrogen production from the slurry were observed at 60 and 75°C (392 and 248 ml H₂ per liter of slurry, respectively). After the termination of the hydrogen evolution, the microflora cultured at 60°C displayed hydrogen-consuming activity, but hydrogen-consuming activity of the microflora cultured at 75°C was not detected, at least for 24 days. At both 60 and 75°C, the main by-product was acetate, and the optimum pH of the slurry for hydrogen production was around neutral. Bacteria related to hydrogen-producing moderate and extreme thermophiles, Clostridium thermocellum and Caldanaerobacter subterraneus, were detected in the slurries cultured at 60 and 75°C, respectively, by denaturing gradient gel electrophoresis analyses, using the V3 region of 16S rDNA.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-006-0647-4</identifier><identifier>PMID: 17021868</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Anaerobic microflora ; Animal wastes ; Animals ; Bacteria, Anaerobic - classification ; Bacteria, Anaerobic - genetics ; Bacteria, Anaerobic - metabolism ; Biological and medical sciences ; Biotechnology ; Cattle ; Clostridium thermocellum ; Clostridium thermocellum - classification ; Clostridium thermocellum - genetics ; Clostridium thermocellum - metabolism ; Cow waste ; Dairy cattle ; Dairy wastes ; DNA, Bacterial - analysis ; Electrophoresis, Polyacrylamide Gel - methods ; Fermentation ; Fundamental and applied biological sciences. 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Without the addition of seed bacteria, hydrogen was produced by simply incubating the slurry, using the microflora within the slurry. Interestingly, two peaks of fermentation temperatures for hydrogen production from the slurry were observed at 60 and 75°C (392 and 248 ml H₂ per liter of slurry, respectively). After the termination of the hydrogen evolution, the microflora cultured at 60°C displayed hydrogen-consuming activity, but hydrogen-consuming activity of the microflora cultured at 75°C was not detected, at least for 24 days. At both 60 and 75°C, the main by-product was acetate, and the optimum pH of the slurry for hydrogen production was around neutral. Bacteria related to hydrogen-producing moderate and extreme thermophiles, Clostridium thermocellum and Caldanaerobacter subterraneus, were detected in the slurries cultured at 60 and 75°C, respectively, by denaturing gradient gel electrophoresis analyses, using the V3 region of 16S rDNA.</description><subject>Anaerobic microflora</subject><subject>Animal wastes</subject><subject>Animals</subject><subject>Bacteria, Anaerobic - classification</subject><subject>Bacteria, Anaerobic - genetics</subject><subject>Bacteria, Anaerobic - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cattle</subject><subject>Clostridium thermocellum</subject><subject>Clostridium thermocellum - classification</subject><subject>Clostridium thermocellum - genetics</subject><subject>Clostridium thermocellum - metabolism</subject><subject>Cow waste</subject><subject>Dairy cattle</subject><subject>Dairy wastes</subject><subject>DNA, Bacterial - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hot Temperature</subject><subject>Hydrogen</subject><subject>Hydrogen - metabolism</subject><subject>Hydrogen production</subject><subject>Manure - microbiology</subject><subject>Molecular Sequence Data</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Slurries</subject><subject>Thermophilic condition</subject><subject>Volatile solids</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0U2LFDEQBuAgiruu_gAvGgS9tabS-TzKsn7Aggfdc0inKzO9dHfGpJthTv51M87IghdPoeCpClUvIS-BvQfG9IfCGJdtw5hqmBK6EY_IJYiW1wrEY3LJQMtGS2suyLNS7hkDbpR6Si5AMw5GmUvy6yZGDAtNkUbME86LX4Y00wWnHWa_rBlpLbeHPqcNznSXU7-GPyTmNNGQ9nTvy4K0jGvOB9od6FqGeUP97DGnbgh0GkJOcUzZ0_2wbIc6ffvXPydPoh8Lvji_V-Tu082P6y_N7bfPX68_3jZBaL00CoPspOwFb3XgXZRgOxO8h9ZjZ4FFpYLU0eiOBRFsCygji0Jh36PkWrZX5N1pbl3g54plcdNQAo6jnzGtxSljlTby_xCs1NYaXeGbf-B9WvNcl3AKrDbKcqgITqheoJSM0e3yMPl8cMDcMUN3ytDVDN0xQydqz6vz4LWbsH_oOIdWwdsz8CX4MWY_h6E8OCM5KHZ0r08u-uT8Jldz950zaOu_ghsr29-Nv6_6</recordid><startdate>20070201</startdate><enddate>20070201</enddate><creator>Yokoyama, Hiroshi</creator><creator>Waki, Miyoko</creator><creator>Moriya, Naoko</creator><creator>Yasuda, Tomoko</creator><creator>Tanaka, Yasuo</creator><creator>Haga, Kiyonori</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20070201</creationdate><title>Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry</title><author>Yokoyama, Hiroshi ; Waki, Miyoko ; Moriya, Naoko ; Yasuda, Tomoko ; Tanaka, Yasuo ; Haga, Kiyonori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-6ec5b55d4237c2bf519b8caa13aeb910f66c57f87b0c4c931e5f0f46edde52753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Anaerobic microflora</topic><topic>Animal wastes</topic><topic>Animals</topic><topic>Bacteria, Anaerobic - classification</topic><topic>Bacteria, Anaerobic - genetics</topic><topic>Bacteria, Anaerobic - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cattle</topic><topic>Clostridium thermocellum</topic><topic>Clostridium thermocellum - classification</topic><topic>Clostridium thermocellum - genetics</topic><topic>Clostridium thermocellum - metabolism</topic><topic>Cow waste</topic><topic>Dairy cattle</topic><topic>Dairy wastes</topic><topic>DNA, Bacterial - analysis</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yokoyama, Hiroshi</au><au>Waki, Miyoko</au><au>Moriya, Naoko</au><au>Yasuda, Tomoko</au><au>Tanaka, Yasuo</au><au>Haga, Kiyonori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2007-02-01</date><risdate>2007</risdate><volume>74</volume><issue>2</issue><spage>474</spage><epage>483</epage><pages>474-483</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>We examined hydrogen production from a dairy cow waste slurry (13.4 g of volatile solids per liter) by batch cultures in a temperature range from 37 to 85°C, using microflora naturally present within the slurry. Without the addition of seed bacteria, hydrogen was produced by simply incubating the slurry, using the microflora within the slurry. Interestingly, two peaks of fermentation temperatures for hydrogen production from the slurry were observed at 60 and 75°C (392 and 248 ml H₂ per liter of slurry, respectively). After the termination of the hydrogen evolution, the microflora cultured at 60°C displayed hydrogen-consuming activity, but hydrogen-consuming activity of the microflora cultured at 75°C was not detected, at least for 24 days. At both 60 and 75°C, the main by-product was acetate, and the optimum pH of the slurry for hydrogen production was around neutral. Bacteria related to hydrogen-producing moderate and extreme thermophiles, Clostridium thermocellum and Caldanaerobacter subterraneus, were detected in the slurries cultured at 60 and 75°C, respectively, by denaturing gradient gel electrophoresis analyses, using the V3 region of 16S rDNA.</abstract><cop>Berlin</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>17021868</pmid><doi>10.1007/s00253-006-0647-4</doi><tpages>10</tpages></addata></record> |
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| subjects | Anaerobic microflora Animal wastes Animals Bacteria, Anaerobic - classification Bacteria, Anaerobic - genetics Bacteria, Anaerobic - metabolism Biological and medical sciences Biotechnology Cattle Clostridium thermocellum Clostridium thermocellum - classification Clostridium thermocellum - genetics Clostridium thermocellum - metabolism Cow waste Dairy cattle Dairy wastes DNA, Bacterial - analysis Electrophoresis, Polyacrylamide Gel - methods Fermentation Fundamental and applied biological sciences. Psychology Hot Temperature Hydrogen Hydrogen - metabolism Hydrogen production Manure - microbiology Molecular Sequence Data RNA, Ribosomal, 16S - genetics Slurries Thermophilic condition Volatile solids |
| title | Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry |
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