Multi-component analysis of marine lipids in fish gonads with emphasis on phospholipids using high resolution NMR spectroscopy
High resolution NMR has been applied for assessment of lipid classes and acyl stereospecific positions of fatty acids in marine phospholipids and triacylglycerols. 1D and 2D NMR techniques in combination with recording of a number of reference standards have been used to interpret the 1H and 13C NMR...
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| Veröffentlicht in: | Chemistry and physics of lipids 2006-10, Vol.144 (1), p.4-16 |
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| creator | Falch, E. Størseth, T.R. Aursand, M. |
| description | High resolution NMR has been applied for assessment of lipid classes and acyl stereospecific positions of fatty acids in marine phospholipids and triacylglycerols. 1D and 2D NMR techniques in combination with recording of a number of reference standards have been used to interpret the
1H and
13C NMR spectra of fish gonads.
13C NMR spectra gave information regarding the polyunsaturated fatty acids (PUFAs) in phosphatidylcholine (PC) and phoshatidylethanolamine (PE). The carbonyl resonances showed that
n
−
3 PUFAs primarily were esterified in the
sn-2 position of PC and PE. The glycerol resonances showed that the PC/PE ratio was higher in roe than in milt and that roe comprised more triacylglycerols than milt. Thin layer chromatography showed that milt contained 2.4 times more cholesterol than roe, which was also found by integrating the
1H NMR spectra. Concentration (mol%) of
n
−
3 fatty acids were calculated from the
1H NMR data and showed 44.8 and 36.3% in roe and milt, respectively. |
| doi_str_mv | 10.1016/j.chemphyslip.2006.05.001 |
| format | Article |
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1H and
13C NMR spectra of fish gonads.
13C NMR spectra gave information regarding the polyunsaturated fatty acids (PUFAs) in phosphatidylcholine (PC) and phoshatidylethanolamine (PE). The carbonyl resonances showed that
n
−
3 PUFAs primarily were esterified in the
sn-2 position of PC and PE. The glycerol resonances showed that the PC/PE ratio was higher in roe than in milt and that roe comprised more triacylglycerols than milt. Thin layer chromatography showed that milt contained 2.4 times more cholesterol than roe, which was also found by integrating the
1H NMR spectra. Concentration (mol%) of
n
−
3 fatty acids were calculated from the
1H NMR data and showed 44.8 and 36.3% in roe and milt, respectively.</description><identifier>ISSN: 0009-3084</identifier><identifier>EISSN: 1873-2941</identifier><identifier>DOI: 10.1016/j.chemphyslip.2006.05.001</identifier><identifier>PMID: 16890212</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Acyl stereospecific position ; Animals ; Carbon Isotopes ; Cholesterol ; DHA ; EPA ; Fatty Acids - analysis ; Fish oil ; Fishes ; Gadus morhua ; Gonads - chemistry ; Lipids - analysis ; Magnetic Resonance Spectroscopy - methods ; n − 3 ; NMR ; Phospholipids ; Phospholipids - analysis ; PUFA</subject><ispartof>Chemistry and physics of lipids, 2006-10, Vol.144 (1), p.4-16</ispartof><rights>2006 Elsevier Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-a2bb635e843def364421a2512a83c18277d776a443251effa575a85bbcd918433</citedby><cites>FETCH-LOGICAL-c375t-a2bb635e843def364421a2512a83c18277d776a443251effa575a85bbcd918433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chemphyslip.2006.05.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16890212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Falch, E.</creatorcontrib><creatorcontrib>Størseth, T.R.</creatorcontrib><creatorcontrib>Aursand, M.</creatorcontrib><title>Multi-component analysis of marine lipids in fish gonads with emphasis on phospholipids using high resolution NMR spectroscopy</title><title>Chemistry and physics of lipids</title><addtitle>Chem Phys Lipids</addtitle><description>High resolution NMR has been applied for assessment of lipid classes and acyl stereospecific positions of fatty acids in marine phospholipids and triacylglycerols. 1D and 2D NMR techniques in combination with recording of a number of reference standards have been used to interpret the
1H and
13C NMR spectra of fish gonads.
13C NMR spectra gave information regarding the polyunsaturated fatty acids (PUFAs) in phosphatidylcholine (PC) and phoshatidylethanolamine (PE). The carbonyl resonances showed that
n
−
3 PUFAs primarily were esterified in the
sn-2 position of PC and PE. The glycerol resonances showed that the PC/PE ratio was higher in roe than in milt and that roe comprised more triacylglycerols than milt. Thin layer chromatography showed that milt contained 2.4 times more cholesterol than roe, which was also found by integrating the
1H NMR spectra. Concentration (mol%) of
n
−
3 fatty acids were calculated from the
1H NMR data and showed 44.8 and 36.3% in roe and milt, respectively.</description><subject>Acyl stereospecific position</subject><subject>Animals</subject><subject>Carbon Isotopes</subject><subject>Cholesterol</subject><subject>DHA</subject><subject>EPA</subject><subject>Fatty Acids - analysis</subject><subject>Fish oil</subject><subject>Fishes</subject><subject>Gadus morhua</subject><subject>Gonads - chemistry</subject><subject>Lipids - analysis</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>n − 3</subject><subject>NMR</subject><subject>Phospholipids</subject><subject>Phospholipids - analysis</subject><subject>PUFA</subject><issn>0009-3084</issn><issn>1873-2941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1r3DAQhkVJaDbb_oWiXHKzqw_LH8eyJG0gHxDas5Dl8VqLLbkeO2Ev_e3RdhfaYw5CzPCM3tFDyBVnKWc8_7pLbQfD2O2xd2MqGMtTplLG-Aey4mUhE1Fl_IysGGNVIlmZXZBLxF0smVL8I7ngeVkxwcWK_HlY-tklNgxj8OBnarzp9-iQhpYOZnIeaAxxDVLnaeuwo9vgTSxf3dzRwxbmL-3p2AWM50Qv6PyWdm7b0Qkw9MvsIvP48ExxBDtPAW0Y95_IeWt6hM-ne01-3d783PxI7p--322-3SdWFmpOjKjrXCooM9lAK_MsE9wIxYUppeWlKIqmKHKTZTI2oW2NKpQpVV3bpuJxSK7J9fHdcQq_F8BZDw4t9L3xEBbU0UcuD-rWpDqCNm6IE7R6nFz0sNec6YN8vdP_ydcH-ZopHeXH2S-nkKUeoPk3ebIdgc0RgPjVFweTRuvAW2jcFJ3oJrh3xLwBdq-exg</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Falch, E.</creator><creator>Størseth, T.R.</creator><creator>Aursand, M.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>Multi-component analysis of marine lipids in fish gonads with emphasis on phospholipids using high resolution NMR spectroscopy</title><author>Falch, E. ; Størseth, T.R. ; Aursand, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-a2bb635e843def364421a2512a83c18277d776a443251effa575a85bbcd918433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acyl stereospecific position</topic><topic>Animals</topic><topic>Carbon Isotopes</topic><topic>Cholesterol</topic><topic>DHA</topic><topic>EPA</topic><topic>Fatty Acids - analysis</topic><topic>Fish oil</topic><topic>Fishes</topic><topic>Gadus morhua</topic><topic>Gonads - chemistry</topic><topic>Lipids - analysis</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>n − 3</topic><topic>NMR</topic><topic>Phospholipids</topic><topic>Phospholipids - analysis</topic><topic>PUFA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Falch, E.</creatorcontrib><creatorcontrib>Størseth, T.R.</creatorcontrib><creatorcontrib>Aursand, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry and physics of lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Falch, E.</au><au>Størseth, T.R.</au><au>Aursand, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multi-component analysis of marine lipids in fish gonads with emphasis on phospholipids using high resolution NMR spectroscopy</atitle><jtitle>Chemistry and physics of lipids</jtitle><addtitle>Chem Phys Lipids</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>144</volume><issue>1</issue><spage>4</spage><epage>16</epage><pages>4-16</pages><issn>0009-3084</issn><eissn>1873-2941</eissn><abstract>High resolution NMR has been applied for assessment of lipid classes and acyl stereospecific positions of fatty acids in marine phospholipids and triacylglycerols. 1D and 2D NMR techniques in combination with recording of a number of reference standards have been used to interpret the
1H and
13C NMR spectra of fish gonads.
13C NMR spectra gave information regarding the polyunsaturated fatty acids (PUFAs) in phosphatidylcholine (PC) and phoshatidylethanolamine (PE). The carbonyl resonances showed that
n
−
3 PUFAs primarily were esterified in the
sn-2 position of PC and PE. The glycerol resonances showed that the PC/PE ratio was higher in roe than in milt and that roe comprised more triacylglycerols than milt. Thin layer chromatography showed that milt contained 2.4 times more cholesterol than roe, which was also found by integrating the
1H NMR spectra. Concentration (mol%) of
n
−
3 fatty acids were calculated from the
1H NMR data and showed 44.8 and 36.3% in roe and milt, respectively.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>16890212</pmid><doi>10.1016/j.chemphyslip.2006.05.001</doi><tpages>13</tpages></addata></record> |
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| ispartof | Chemistry and physics of lipids, 2006-10, Vol.144 (1), p.4-16 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acyl stereospecific position Animals Carbon Isotopes Cholesterol DHA EPA Fatty Acids - analysis Fish oil Fishes Gadus morhua Gonads - chemistry Lipids - analysis Magnetic Resonance Spectroscopy - methods n − 3 NMR Phospholipids Phospholipids - analysis PUFA |
| title | Multi-component analysis of marine lipids in fish gonads with emphasis on phospholipids using high resolution NMR spectroscopy |
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