Human Hydroxysteroid (17-β) Dehydrogenase 1 Expression Enhances Estrogen Sensitivity of MCF-7 Breast Cancer Cell Xenografts
Hydroxysteroid (17-β) dehydrogenase 1 (HSD17B1) catalyzes the conversion between estrone (E1) and estradiol (E2). The reaction is reversible in vitro, but the data in cultured cells suggest that E2 production is the predominant reaction in physiological conditions. However, the hypothesis has not be...
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description | Hydroxysteroid (17-β) dehydrogenase 1 (HSD17B1) catalyzes the conversion between estrone (E1) and estradiol (E2). The reaction is reversible in vitro, but the data in cultured cells suggest that E2 production is the predominant reaction in physiological conditions. However, the hypothesis has not been verified in vivo. In the present study, estrogen-dependent MCF-7 human breast cancer cells were stably transfected with an expression plasmid for human HSD17B1. The enzyme efficiently converted E1 to E2 and enhanced the estrogen-dependent growth of cultured MCF-7 cells in the presence of hormonally less active E1. The HSD17B1-expressing cells also formed estrogen-dependent tumors in immunodeficient nude mice. After treating the mice with an appropriate dose of the substrate (E1, 0.1 μmol/kg·d), a marked difference in tumor growth was observed between nontransfected and HSD17B1-transfected MCF-7 cells, mean tumor weights at the end of E1 treatment being 23.2 and 130.4 mg, respectively. Furthermore, estrogen-dependent growth of the HSD17B1-expressing xenografts in the presence of E1 was markedly inhibited by administering 5 μmol/kg·d of a specific HSD17B1 inhibitor. After a 4-wk treatment, the tumor size was reduced by 59.8% as compared with the nontreated tumors, whereas the uterine growth of the mice was not affected by the HSD17B1 inhibitor used. This was in line with the induction of apoptosis of the tumors. The results evidently show that estrogenic response for E1 is enhanced by the local action of HSD17B1 in vivo, and thus, the enzyme is a potential target for pharmacological inhibition of estrogen action. |
doi_str_mv | 10.1210/en.2006-0778 |
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The reaction is reversible in vitro, but the data in cultured cells suggest that E2 production is the predominant reaction in physiological conditions. However, the hypothesis has not been verified in vivo. In the present study, estrogen-dependent MCF-7 human breast cancer cells were stably transfected with an expression plasmid for human HSD17B1. The enzyme efficiently converted E1 to E2 and enhanced the estrogen-dependent growth of cultured MCF-7 cells in the presence of hormonally less active E1. The HSD17B1-expressing cells also formed estrogen-dependent tumors in immunodeficient nude mice. After treating the mice with an appropriate dose of the substrate (E1, 0.1 μmol/kg·d), a marked difference in tumor growth was observed between nontransfected and HSD17B1-transfected MCF-7 cells, mean tumor weights at the end of E1 treatment being 23.2 and 130.4 mg, respectively. Furthermore, estrogen-dependent growth of the HSD17B1-expressing xenografts in the presence of E1 was markedly inhibited by administering 5 μmol/kg·d of a specific HSD17B1 inhibitor. After a 4-wk treatment, the tumor size was reduced by 59.8% as compared with the nontreated tumors, whereas the uterine growth of the mice was not affected by the HSD17B1 inhibitor used. This was in line with the induction of apoptosis of the tumors. The results evidently show that estrogenic response for E1 is enhanced by the local action of HSD17B1 in vivo, and thus, the enzyme is a potential target for pharmacological inhibition of estrogen action.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2006-0778</identifier><identifier>PMID: 16916945</identifier><identifier>CODEN: ENDOAO</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Animals ; Apoptosis ; Biological and medical sciences ; Breast cancer ; Cell Line, Tumor ; Dehydrogenase ; Dehydrogenases ; Enzyme Inhibitors - pharmacology ; Enzymes ; Estradiol Dehydrogenases - antagonists & inhibitors ; Estradiol Dehydrogenases - physiology ; Estrogen Receptor alpha - physiology ; Estrogens ; Estrogens - metabolism ; Estrogens - pharmacology ; Estrone ; Female ; Fundamental and applied biological sciences. Psychology ; Gynecology. Andrology. Obstetrics ; Humans ; Hydroxysteroids ; Immunodeficiency ; In vivo methods and tests ; Inhibitors ; Mammary gland diseases ; Mammary Neoplasms, Experimental - drug therapy ; Mammary Neoplasms, Experimental - enzymology ; Mammary Neoplasms, Experimental - pathology ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Neoplasm Transplantation ; Neoplasms, Hormone-Dependent - drug therapy ; Neoplasms, Hormone-Dependent - enzymology ; Neoplasms, Hormone-Dependent - pathology ; Sex hormones ; Transplantation, Heterologous ; Tumors ; Vertebrates: endocrinology ; Xenoestrogens ; Xenografts ; Xenotransplantation</subject><ispartof>Endocrinology (Philadelphia), 2006-11, Vol.147 (11), p.5333-5339</ispartof><rights>Copyright © 2006 by The Endocrine Society 2006</rights><rights>2007 INIST-CNRS</rights><rights>Copyright © 2006 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-be1cbb775d1db090374d45e4a87ffae34344233e6567561b936d4ed6b79cce213</citedby><cites>FETCH-LOGICAL-c423t-be1cbb775d1db090374d45e4a87ffae34344233e6567561b936d4ed6b79cce213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18217492$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16916945$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Husen, Bettina</creatorcontrib><creatorcontrib>Huhtinen, Kaisa</creatorcontrib><creatorcontrib>Saloniemi, Taija</creatorcontrib><creatorcontrib>Messinger, Josef</creatorcontrib><creatorcontrib>Thole, Hubert H</creatorcontrib><creatorcontrib>Poutanen, Matti</creatorcontrib><title>Human Hydroxysteroid (17-β) Dehydrogenase 1 Expression Enhances Estrogen Sensitivity of MCF-7 Breast Cancer Cell Xenografts</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>Hydroxysteroid (17-β) dehydrogenase 1 (HSD17B1) catalyzes the conversion between estrone (E1) and estradiol (E2). The reaction is reversible in vitro, but the data in cultured cells suggest that E2 production is the predominant reaction in physiological conditions. However, the hypothesis has not been verified in vivo. In the present study, estrogen-dependent MCF-7 human breast cancer cells were stably transfected with an expression plasmid for human HSD17B1. The enzyme efficiently converted E1 to E2 and enhanced the estrogen-dependent growth of cultured MCF-7 cells in the presence of hormonally less active E1. The HSD17B1-expressing cells also formed estrogen-dependent tumors in immunodeficient nude mice. After treating the mice with an appropriate dose of the substrate (E1, 0.1 μmol/kg·d), a marked difference in tumor growth was observed between nontransfected and HSD17B1-transfected MCF-7 cells, mean tumor weights at the end of E1 treatment being 23.2 and 130.4 mg, respectively. Furthermore, estrogen-dependent growth of the HSD17B1-expressing xenografts in the presence of E1 was markedly inhibited by administering 5 μmol/kg·d of a specific HSD17B1 inhibitor. After a 4-wk treatment, the tumor size was reduced by 59.8% as compared with the nontreated tumors, whereas the uterine growth of the mice was not affected by the HSD17B1 inhibitor used. This was in line with the induction of apoptosis of the tumors. The results evidently show that estrogenic response for E1 is enhanced by the local action of HSD17B1 in vivo, and thus, the enzyme is a potential target for pharmacological inhibition of estrogen action.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Breast cancer</subject><subject>Cell Line, Tumor</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Enzymes</subject><subject>Estradiol Dehydrogenases - antagonists & inhibitors</subject><subject>Estradiol Dehydrogenases - physiology</subject><subject>Estrogen Receptor alpha - physiology</subject><subject>Estrogens</subject><subject>Estrogens - metabolism</subject><subject>Estrogens - pharmacology</subject><subject>Estrone</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Hydroxysteroids</subject><subject>Immunodeficiency</subject><subject>In vivo methods and tests</subject><subject>Inhibitors</subject><subject>Mammary gland diseases</subject><subject>Mammary Neoplasms, Experimental - drug therapy</subject><subject>Mammary Neoplasms, Experimental - enzymology</subject><subject>Mammary Neoplasms, Experimental - pathology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neoplasm Transplantation</subject><subject>Neoplasms, Hormone-Dependent - drug therapy</subject><subject>Neoplasms, Hormone-Dependent - enzymology</subject><subject>Neoplasms, Hormone-Dependent - pathology</subject><subject>Sex hormones</subject><subject>Transplantation, Heterologous</subject><subject>Tumors</subject><subject>Vertebrates: endocrinology</subject><subject>Xenoestrogens</subject><subject>Xenografts</subject><subject>Xenotransplantation</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10d2K1DAUB_AgijuO3nktAfEL7Jo0adJeap11hBUvVPCupM3pbpaZZDanlR3wqXwQn8l0pzAgCoEQ8uOck_wJeczZKc85ewP-NGdMZUzr8g5Z8EoWmeaa3SULxrjIdJ7rE_IA8SodpZTiPjnhqkpLFgvycz1ujafrvY3hZo8DxOAsfcl19vvXK_oeLqeLC_AGgXK6utlFQHTB05W_NL4DpCscbgX9Ah7d4H64YU9DTz_VZ5mm7yIYHGg92Uhr2Gzod_DhIpp-wIfkXm82CI_mfUm-na2-1uvs_POHj_Xb86yTuRiyFnjXtloXltuWVUxoaWUB0pS67w0IKWRyAlShdKF4WwllJVjV6qrrIOdiSZ4f6u5iuB4Bh2brsEuzGA9hxEaVlcpZqrEkT_-CV2GMPs3WCC5YoaUqi6ReH1QXA2KEvtlFtzVx33DWTJk04Jspk2bKJPEnc9Gx3YI94jmEBJ7NwGBnNn1Mn-Xw6Mqca1lN0704uDDu_tcym1uKgwRvQxedh9vcjq_556B_AI6usNY</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Husen, Bettina</creator><creator>Huhtinen, Kaisa</creator><creator>Saloniemi, Taija</creator><creator>Messinger, Josef</creator><creator>Thole, Hubert H</creator><creator>Poutanen, Matti</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20061101</creationdate><title>Human Hydroxysteroid (17-β) Dehydrogenase 1 Expression Enhances Estrogen Sensitivity of MCF-7 Breast Cancer Cell Xenografts</title><author>Husen, Bettina ; Huhtinen, Kaisa ; Saloniemi, Taija ; Messinger, Josef ; Thole, Hubert H ; Poutanen, Matti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-be1cbb775d1db090374d45e4a87ffae34344233e6567561b936d4ed6b79cce213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Breast cancer</topic><topic>Cell Line, Tumor</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Enzymes</topic><topic>Estradiol Dehydrogenases - antagonists & inhibitors</topic><topic>Estradiol Dehydrogenases - physiology</topic><topic>Estrogen Receptor alpha - physiology</topic><topic>Estrogens</topic><topic>Estrogens - metabolism</topic><topic>Estrogens - pharmacology</topic><topic>Estrone</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Hydroxysteroids</topic><topic>Immunodeficiency</topic><topic>In vivo methods and tests</topic><topic>Inhibitors</topic><topic>Mammary gland diseases</topic><topic>Mammary Neoplasms, Experimental - drug therapy</topic><topic>Mammary Neoplasms, Experimental - enzymology</topic><topic>Mammary Neoplasms, Experimental - pathology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neoplasm Transplantation</topic><topic>Neoplasms, Hormone-Dependent - drug therapy</topic><topic>Neoplasms, Hormone-Dependent - enzymology</topic><topic>Neoplasms, Hormone-Dependent - pathology</topic><topic>Sex hormones</topic><topic>Transplantation, Heterologous</topic><topic>Tumors</topic><topic>Vertebrates: endocrinology</topic><topic>Xenoestrogens</topic><topic>Xenografts</topic><topic>Xenotransplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Husen, Bettina</creatorcontrib><creatorcontrib>Huhtinen, Kaisa</creatorcontrib><creatorcontrib>Saloniemi, Taija</creatorcontrib><creatorcontrib>Messinger, Josef</creatorcontrib><creatorcontrib>Thole, Hubert H</creatorcontrib><creatorcontrib>Poutanen, Matti</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Husen, Bettina</au><au>Huhtinen, Kaisa</au><au>Saloniemi, Taija</au><au>Messinger, Josef</au><au>Thole, Hubert H</au><au>Poutanen, Matti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Hydroxysteroid (17-β) Dehydrogenase 1 Expression Enhances Estrogen Sensitivity of MCF-7 Breast Cancer Cell Xenografts</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>147</volume><issue>11</issue><spage>5333</spage><epage>5339</epage><pages>5333-5339</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><coden>ENDOAO</coden><abstract>Hydroxysteroid (17-β) dehydrogenase 1 (HSD17B1) catalyzes the conversion between estrone (E1) and estradiol (E2). The reaction is reversible in vitro, but the data in cultured cells suggest that E2 production is the predominant reaction in physiological conditions. However, the hypothesis has not been verified in vivo. In the present study, estrogen-dependent MCF-7 human breast cancer cells were stably transfected with an expression plasmid for human HSD17B1. The enzyme efficiently converted E1 to E2 and enhanced the estrogen-dependent growth of cultured MCF-7 cells in the presence of hormonally less active E1. The HSD17B1-expressing cells also formed estrogen-dependent tumors in immunodeficient nude mice. After treating the mice with an appropriate dose of the substrate (E1, 0.1 μmol/kg·d), a marked difference in tumor growth was observed between nontransfected and HSD17B1-transfected MCF-7 cells, mean tumor weights at the end of E1 treatment being 23.2 and 130.4 mg, respectively. Furthermore, estrogen-dependent growth of the HSD17B1-expressing xenografts in the presence of E1 was markedly inhibited by administering 5 μmol/kg·d of a specific HSD17B1 inhibitor. After a 4-wk treatment, the tumor size was reduced by 59.8% as compared with the nontreated tumors, whereas the uterine growth of the mice was not affected by the HSD17B1 inhibitor used. This was in line with the induction of apoptosis of the tumors. The results evidently show that estrogenic response for E1 is enhanced by the local action of HSD17B1 in vivo, and thus, the enzyme is a potential target for pharmacological inhibition of estrogen action.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>16916945</pmid><doi>10.1210/en.2006-0778</doi><tpages>7</tpages></addata></record> |
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subjects | Animals Apoptosis Biological and medical sciences Breast cancer Cell Line, Tumor Dehydrogenase Dehydrogenases Enzyme Inhibitors - pharmacology Enzymes Estradiol Dehydrogenases - antagonists & inhibitors Estradiol Dehydrogenases - physiology Estrogen Receptor alpha - physiology Estrogens Estrogens - metabolism Estrogens - pharmacology Estrone Female Fundamental and applied biological sciences. Psychology Gynecology. Andrology. Obstetrics Humans Hydroxysteroids Immunodeficiency In vivo methods and tests Inhibitors Mammary gland diseases Mammary Neoplasms, Experimental - drug therapy Mammary Neoplasms, Experimental - enzymology Mammary Neoplasms, Experimental - pathology Medical sciences Mice Mice, Inbred BALB C Neoplasm Transplantation Neoplasms, Hormone-Dependent - drug therapy Neoplasms, Hormone-Dependent - enzymology Neoplasms, Hormone-Dependent - pathology Sex hormones Transplantation, Heterologous Tumors Vertebrates: endocrinology Xenoestrogens Xenografts Xenotransplantation |
title | Human Hydroxysteroid (17-β) Dehydrogenase 1 Expression Enhances Estrogen Sensitivity of MCF-7 Breast Cancer Cell Xenografts |
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