Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo
To study cellular characteristics of human cementoblasts using a cellular model is important for understanding the mechanisms of homeostasis and regeneration of periodontal tissues. However, at present no immortalized human cementoblast cell line has been established due to limitation of the life sp...
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| Veröffentlicht in: | Bone (New York, N.Y.) N.Y.), 2006-11, Vol.39 (5), p.1035-1042 |
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| creator | Kitagawa, Masae Tahara, Hidetoshi Kitagawa, Shoji Oka, Hiroko Kudo, Yasusei Sato, Sunao Ogawa, Ikuko Miyaichi, Mutsumi Takata, Takashi |
| description | To study cellular characteristics of human cementoblasts using a cellular model is important for understanding the mechanisms of homeostasis and regeneration of periodontal tissues. However, at present no immortalized human cementoblast cell line has been established due to limitation of the life span. In the present study, therefore, we attempted to establish human cementoblast-like cell lines by transfection with telomerase catalytic subunit hTERT gene. Two stable clones (HCEM-1 and -2) with high telomerase activity were obtained and they grew over 200 population doublings without significant growth retardation. The expression of mRNA for differentiation markers, type I collagen, alkaline phosphatase (ALP), runt-related transcription factor 2, osteocalcin, bone sialoprotein and cementum-derived protein was revealed in these clones by RT-PCR. Moreover, these cells showed high ALP activity and calcified nodule formation in vitro. Interestingly, HCEM-2 showed cementum like formation on the surface of hydroxyapatites granules by subcutaneous transplantation into immunodeficient mice with hydroxyapatite granules. Thus, we established human cementoblast-like cell lines. We suggest that HCEM cell lines can be useful cell models for investigating the characteristics of human cementoblasts. |
| doi_str_mv | 10.1016/j.bone.2006.05.022 |
| format | Article |
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However, at present no immortalized human cementoblast cell line has been established due to limitation of the life span. In the present study, therefore, we attempted to establish human cementoblast-like cell lines by transfection with telomerase catalytic subunit hTERT gene. Two stable clones (HCEM-1 and -2) with high telomerase activity were obtained and they grew over 200 population doublings without significant growth retardation. The expression of mRNA for differentiation markers, type I collagen, alkaline phosphatase (ALP), runt-related transcription factor 2, osteocalcin, bone sialoprotein and cementum-derived protein was revealed in these clones by RT-PCR. Moreover, these cells showed high ALP activity and calcified nodule formation in vitro. Interestingly, HCEM-2 showed cementum like formation on the surface of hydroxyapatites granules by subcutaneous transplantation into immunodeficient mice with hydroxyapatite granules. Thus, we established human cementoblast-like cell lines. We suggest that HCEM cell lines can be useful cell models for investigating the characteristics of human cementoblasts.</description><identifier>ISSN: 8756-3282</identifier><identifier>EISSN: 1873-2763</identifier><identifier>DOI: 10.1016/j.bone.2006.05.022</identifier><identifier>PMID: 16857433</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Alkaline Phosphatase - genetics ; Animals ; Biological and medical sciences ; Cell Line ; Cell Transplantation - methods ; Cementoblast ; Collagen Type I - genetics ; Core Binding Factor Alpha 1 Subunit - genetics ; Dental Cementum - cytology ; Dental Cementum - metabolism ; Dental Cementum - transplantation ; Differentiation ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; hTERT ; Humans ; Immortalization ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Osteocalcin - genetics ; Periodontal ligament ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Transplantation, Heterologous ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Bone (New York, N.Y.), 2006-11, Vol.39 (5), p.1035-1042</ispartof><rights>2006 Elsevier Inc.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-26c84a54cc5fadbcd2e2ae407caf2e8e75ee4fdc0f7bed591b41484e7de173a33</citedby><cites>FETCH-LOGICAL-c525t-26c84a54cc5fadbcd2e2ae407caf2e8e75ee4fdc0f7bed591b41484e7de173a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S8756328206005539$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18237135$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16857433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitagawa, Masae</creatorcontrib><creatorcontrib>Tahara, Hidetoshi</creatorcontrib><creatorcontrib>Kitagawa, Shoji</creatorcontrib><creatorcontrib>Oka, Hiroko</creatorcontrib><creatorcontrib>Kudo, Yasusei</creatorcontrib><creatorcontrib>Sato, Sunao</creatorcontrib><creatorcontrib>Ogawa, Ikuko</creatorcontrib><creatorcontrib>Miyaichi, Mutsumi</creatorcontrib><creatorcontrib>Takata, Takashi</creatorcontrib><title>Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo</title><title>Bone (New York, N.Y.)</title><addtitle>Bone</addtitle><description>To study cellular characteristics of human cementoblasts using a cellular model is important for understanding the mechanisms of homeostasis and regeneration of periodontal tissues. However, at present no immortalized human cementoblast cell line has been established due to limitation of the life span. In the present study, therefore, we attempted to establish human cementoblast-like cell lines by transfection with telomerase catalytic subunit hTERT gene. Two stable clones (HCEM-1 and -2) with high telomerase activity were obtained and they grew over 200 population doublings without significant growth retardation. The expression of mRNA for differentiation markers, type I collagen, alkaline phosphatase (ALP), runt-related transcription factor 2, osteocalcin, bone sialoprotein and cementum-derived protein was revealed in these clones by RT-PCR. Moreover, these cells showed high ALP activity and calcified nodule formation in vitro. Interestingly, HCEM-2 showed cementum like formation on the surface of hydroxyapatites granules by subcutaneous transplantation into immunodeficient mice with hydroxyapatite granules. Thus, we established human cementoblast-like cell lines. We suggest that HCEM cell lines can be useful cell models for investigating the characteristics of human cementoblasts.</description><subject>Alkaline Phosphatase - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell Transplantation - methods</subject><subject>Cementoblast</subject><subject>Collagen Type I - genetics</subject><subject>Core Binding Factor Alpha 1 Subunit - genetics</subject><subject>Dental Cementum - cytology</subject><subject>Dental Cementum - metabolism</subject><subject>Dental Cementum - transplantation</subject><subject>Differentiation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>hTERT</subject><subject>Humans</subject><subject>Immortalization</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Osteocalcin - genetics</subject><subject>Periodontal ligament</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Transplantation, Heterologous</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>8756-3282</issn><issn>1873-2763</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c-P1CAUB3BiNO7s6j_gwXDRWys_SmESL2aiq8kmXvRMKDwcxhZWaCdZ_3qp02RveiKQz3t5jy9CryhpKaH9u1M7pAgtI6RviWgJY0_QjirJGyZ7_hTtlBR9w5liV-i6lBMhhO8lfY6uaK-E7DjfoXw4mmzsDDn8NnNIESePocxmGEM5gsMWJohzGkZT5mYMP6G-jCMeQ4SCfU4TPi6TiZtbpmpiiD_-qoJDxOcw54RNdJfLOb1Az7wZC7zczhv0_dPHb4fPzd3X2y-HD3eNFUzMDeut6ozorBXeuME6BsxAR6Q1noECKQA67yzxcgAn9nToaKc6kA6o5IbzG_T20vc-p19L3UlPoaxjmQhpKbpX-54KIf8L6Z6r2nKF7AJtTqVk8Po-h8nkB02JXiPRJ71GotdINBG6RlKLXm_dl2EC91iyZVDBmw2YYs3os4k2lEenGJeUi-reXxzUTzsHyLrYANGCCxnsrF0K_5rjDx_qrPc</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Kitagawa, Masae</creator><creator>Tahara, Hidetoshi</creator><creator>Kitagawa, Shoji</creator><creator>Oka, Hiroko</creator><creator>Kudo, Yasusei</creator><creator>Sato, Sunao</creator><creator>Ogawa, Ikuko</creator><creator>Miyaichi, Mutsumi</creator><creator>Takata, Takashi</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>20061101</creationdate><title>Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo</title><author>Kitagawa, Masae ; Tahara, Hidetoshi ; Kitagawa, Shoji ; Oka, Hiroko ; Kudo, Yasusei ; Sato, Sunao ; Ogawa, Ikuko ; Miyaichi, Mutsumi ; Takata, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-26c84a54cc5fadbcd2e2ae407caf2e8e75ee4fdc0f7bed591b41484e7de173a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Alkaline Phosphatase - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell Transplantation - methods</topic><topic>Cementoblast</topic><topic>Collagen Type I - genetics</topic><topic>Core Binding Factor Alpha 1 Subunit - genetics</topic><topic>Dental Cementum - cytology</topic><topic>Dental Cementum - metabolism</topic><topic>Dental Cementum - transplantation</topic><topic>Differentiation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - genetics</topic><topic>hTERT</topic><topic>Humans</topic><topic>Immortalization</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Osteocalcin - genetics</topic><topic>Periodontal ligament</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Transplantation, Heterologous</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitagawa, Masae</creatorcontrib><creatorcontrib>Tahara, Hidetoshi</creatorcontrib><creatorcontrib>Kitagawa, Shoji</creatorcontrib><creatorcontrib>Oka, Hiroko</creatorcontrib><creatorcontrib>Kudo, Yasusei</creatorcontrib><creatorcontrib>Sato, Sunao</creatorcontrib><creatorcontrib>Ogawa, Ikuko</creatorcontrib><creatorcontrib>Miyaichi, Mutsumi</creatorcontrib><creatorcontrib>Takata, Takashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bone (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitagawa, Masae</au><au>Tahara, Hidetoshi</au><au>Kitagawa, Shoji</au><au>Oka, Hiroko</au><au>Kudo, Yasusei</au><au>Sato, Sunao</au><au>Ogawa, Ikuko</au><au>Miyaichi, Mutsumi</au><au>Takata, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo</atitle><jtitle>Bone (New York, N.Y.)</jtitle><addtitle>Bone</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>39</volume><issue>5</issue><spage>1035</spage><epage>1042</epage><pages>1035-1042</pages><issn>8756-3282</issn><eissn>1873-2763</eissn><abstract>To study cellular characteristics of human cementoblasts using a cellular model is important for understanding the mechanisms of homeostasis and regeneration of periodontal tissues. However, at present no immortalized human cementoblast cell line has been established due to limitation of the life span. In the present study, therefore, we attempted to establish human cementoblast-like cell lines by transfection with telomerase catalytic subunit hTERT gene. Two stable clones (HCEM-1 and -2) with high telomerase activity were obtained and they grew over 200 population doublings without significant growth retardation. The expression of mRNA for differentiation markers, type I collagen, alkaline phosphatase (ALP), runt-related transcription factor 2, osteocalcin, bone sialoprotein and cementum-derived protein was revealed in these clones by RT-PCR. Moreover, these cells showed high ALP activity and calcified nodule formation in vitro. Interestingly, HCEM-2 showed cementum like formation on the surface of hydroxyapatites granules by subcutaneous transplantation into immunodeficient mice with hydroxyapatite granules. Thus, we established human cementoblast-like cell lines. We suggest that HCEM cell lines can be useful cell models for investigating the characteristics of human cementoblasts.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16857433</pmid><doi>10.1016/j.bone.2006.05.022</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alkaline Phosphatase - genetics Animals Biological and medical sciences Cell Line Cell Transplantation - methods Cementoblast Collagen Type I - genetics Core Binding Factor Alpha 1 Subunit - genetics Dental Cementum - cytology Dental Cementum - metabolism Dental Cementum - transplantation Differentiation Fundamental and applied biological sciences. Psychology Gene Expression - genetics hTERT Humans Immortalization Male Mice Mice, Inbred BALB C Mice, Nude Osteocalcin - genetics Periodontal ligament Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Transplantation, Heterologous Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo |
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