Interactions of highly and low virulent Mycoplasma hyopneumoniae isolates with the respiratory tract of pigs
Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia, a chronic nonfatal disease affecting pigs of all ages. To obtain better insight in the mechanisms responsible for differences in virulence between highly and low virulent M. hyopneumoniae isolates, 23 caesarean-derived, c...
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| Veröffentlicht in: | Veterinary microbiology 2007-02, Vol.120 (1), p.87-95 |
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| creator | Meyns, T. Maes, D. Calus, D. Ribbens, S. Dewulf, J. Chiers, K. de Kruif, A. Cox, E. Decostere, A. Haesebrouck, F. |
| description | Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia, a chronic nonfatal disease affecting pigs of all ages. To obtain better insight in the mechanisms responsible for differences in virulence between highly and low virulent
M. hyopneumoniae isolates, 23 caesarean-derived, colostrum-deprived piglets were randomly assigned to three groups. Groups 1 and 2 consisted of nine animals each, which were intratracheally inoculated at 1 week of age with a highly or a low virulent isolate of
M. hyopneumoniae, respectively. The remaining five animals were inoculated with sterile culture medium. Animals were euthanized at 5, 10, 15 and 28 days post-inoculation (DPI). Animals inoculated with the highly virulent isolate had more neutrophils in BAL fluid at 10, 15 and 28
DPI compared to the other groups. At 10 and 15
DPI, animals in the highly virulent group had significantly higher concentrations of TNF-α in BAL fluid. IL-1β concentration in this group was higher at 5 and 28
DPI compared to the other groups. From 10
DPI onwards, significantly higher titres of
M. hyopneumoniae were detected in the BAL fluid of animals inoculated with the highly virulent isolate compared to animals inoculated with the low virulent isolate. Additionally, the
in vitro generation time of the highly virulent
M. hyopneumoniae isolate was significantly shorter than that of the low virulent isolate. The present study indicates that the difference in pathogenicity between the highly and low virulent isolates is associated with a faster
in vitro growth, a higher capacity to multiply in the lungs and the induction of a more severe inflammation process by the highly virulent isolate. |
| doi_str_mv | 10.1016/j.vetmic.2006.10.010 |
| format | Article |
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M. hyopneumoniae isolates, 23 caesarean-derived, colostrum-deprived piglets were randomly assigned to three groups. Groups 1 and 2 consisted of nine animals each, which were intratracheally inoculated at 1 week of age with a highly or a low virulent isolate of
M. hyopneumoniae, respectively. The remaining five animals were inoculated with sterile culture medium. Animals were euthanized at 5, 10, 15 and 28 days post-inoculation (DPI). Animals inoculated with the highly virulent isolate had more neutrophils in BAL fluid at 10, 15 and 28
DPI compared to the other groups. At 10 and 15
DPI, animals in the highly virulent group had significantly higher concentrations of TNF-α in BAL fluid. IL-1β concentration in this group was higher at 5 and 28
DPI compared to the other groups. From 10
DPI onwards, significantly higher titres of
M. hyopneumoniae were detected in the BAL fluid of animals inoculated with the highly virulent isolate compared to animals inoculated with the low virulent isolate. Additionally, the
in vitro generation time of the highly virulent
M. hyopneumoniae isolate was significantly shorter than that of the low virulent isolate. The present study indicates that the difference in pathogenicity between the highly and low virulent isolates is associated with a faster
in vitro growth, a higher capacity to multiply in the lungs and the induction of a more severe inflammation process by the highly virulent isolate.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2006.10.010</identifier><identifier>PMID: 17123752</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Bacterial - blood ; bacterial colonization ; bacterial pneumonia ; Bacteriology ; Biological and medical sciences ; Bronchoalveolar Lavage Fluid - immunology ; Bronchoalveolar Lavage Fluid - microbiology ; Cytokines - metabolism ; epidemiological studies ; Fundamental and applied biological sciences. Psychology ; immune response ; immunologic factors ; in vitro studies ; in vivo studies ; inflammation ; lungs ; microbial growth ; Microbiology ; Miscellaneous ; Mycoplasma hyopneumoniae ; Mycoplasma hyopneumoniae - growth & development ; Mycoplasma hyopneumoniae - immunology ; Mycoplasma hyopneumoniae - isolation & purification ; Mycoplasma hyopneumoniae - pathogenicity ; Pathogenesis ; pathogenicity ; physiological response ; Pneumonia of Swine, Mycoplasmal - immunology ; Pneumonia of Swine, Mycoplasmal - pathology ; Pneumonia of Swine, Mycoplasmal - virology ; Respiratory Tract Diseases - immunology ; Respiratory Tract Diseases - pathology ; Respiratory Tract Diseases - veterinary ; Respiratory Tract Diseases - virology ; strains ; Swine ; swine diseases ; Time Factors ; Virulence</subject><ispartof>Veterinary microbiology, 2007-02, Vol.120 (1), p.87-95</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-78def7893029204237ba2d04733d7eed98ce7f39fb76d75a93d8e2dc2259a9b53</citedby><cites>FETCH-LOGICAL-c445t-78def7893029204237ba2d04733d7eed98ce7f39fb76d75a93d8e2dc2259a9b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113506003907$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18497893$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17123752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meyns, T.</creatorcontrib><creatorcontrib>Maes, D.</creatorcontrib><creatorcontrib>Calus, D.</creatorcontrib><creatorcontrib>Ribbens, S.</creatorcontrib><creatorcontrib>Dewulf, J.</creatorcontrib><creatorcontrib>Chiers, K.</creatorcontrib><creatorcontrib>de Kruif, A.</creatorcontrib><creatorcontrib>Cox, E.</creatorcontrib><creatorcontrib>Decostere, A.</creatorcontrib><creatorcontrib>Haesebrouck, F.</creatorcontrib><title>Interactions of highly and low virulent Mycoplasma hyopneumoniae isolates with the respiratory tract of pigs</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia, a chronic nonfatal disease affecting pigs of all ages. To obtain better insight in the mechanisms responsible for differences in virulence between highly and low virulent
M. hyopneumoniae isolates, 23 caesarean-derived, colostrum-deprived piglets were randomly assigned to three groups. Groups 1 and 2 consisted of nine animals each, which were intratracheally inoculated at 1 week of age with a highly or a low virulent isolate of
M. hyopneumoniae, respectively. The remaining five animals were inoculated with sterile culture medium. Animals were euthanized at 5, 10, 15 and 28 days post-inoculation (DPI). Animals inoculated with the highly virulent isolate had more neutrophils in BAL fluid at 10, 15 and 28
DPI compared to the other groups. At 10 and 15
DPI, animals in the highly virulent group had significantly higher concentrations of TNF-α in BAL fluid. IL-1β concentration in this group was higher at 5 and 28
DPI compared to the other groups. From 10
DPI onwards, significantly higher titres of
M. hyopneumoniae were detected in the BAL fluid of animals inoculated with the highly virulent isolate compared to animals inoculated with the low virulent isolate. Additionally, the
in vitro generation time of the highly virulent
M. hyopneumoniae isolate was significantly shorter than that of the low virulent isolate. The present study indicates that the difference in pathogenicity between the highly and low virulent isolates is associated with a faster
in vitro growth, a higher capacity to multiply in the lungs and the induction of a more severe inflammation process by the highly virulent isolate.</description><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>bacterial colonization</subject><subject>bacterial pneumonia</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - immunology</subject><subject>Bronchoalveolar Lavage Fluid - microbiology</subject><subject>Cytokines - metabolism</subject><subject>epidemiological studies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>immune response</subject><subject>immunologic factors</subject><subject>in vitro studies</subject><subject>in vivo studies</subject><subject>inflammation</subject><subject>lungs</subject><subject>microbial growth</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycoplasma hyopneumoniae</subject><subject>Mycoplasma hyopneumoniae - growth & development</subject><subject>Mycoplasma hyopneumoniae - immunology</subject><subject>Mycoplasma hyopneumoniae - isolation & purification</subject><subject>Mycoplasma hyopneumoniae - pathogenicity</subject><subject>Pathogenesis</subject><subject>pathogenicity</subject><subject>physiological response</subject><subject>Pneumonia of Swine, Mycoplasmal - immunology</subject><subject>Pneumonia of Swine, Mycoplasmal - pathology</subject><subject>Pneumonia of Swine, Mycoplasmal - virology</subject><subject>Respiratory Tract Diseases - immunology</subject><subject>Respiratory Tract Diseases - pathology</subject><subject>Respiratory Tract Diseases - veterinary</subject><subject>Respiratory Tract Diseases - virology</subject><subject>strains</subject><subject>Swine</subject><subject>swine diseases</subject><subject>Time Factors</subject><subject>Virulence</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1TAYhYMoznX0H4hmo7te89E2zUYYBj8GRlzorENu8vY2l7SpSXqH_ntbemF2unrh5TmHwzkIvaVkTwmtP532Z8i9M3tGSL289oSSZ2hHG8ELVpXsOdoRLpqCUl5doVcpnQghpazJS3RFBWVcVGyH_N2QIWqTXRgSDi3u3LHzM9aDxT484rOLk4ch4x-zCaPXqde4m8M4wNSHwWnALgWvMyT86HKHcwc4Qhpd1DnEGefVe_Ud3TG9Ri9a7RO8udxr9PD1y-_b78X9z293tzf3hSnLKheisdCKRnLCJCPlkvSgmSWl4NwKACsbA6Llsj2I2opKS24bYNYwVkktDxW_Rh833zGGPxOkrHqXDHivBwhTUnUjq7pi_L8glbIRNZcLWG6giSGlCK0ao-t1nBUlap1DndQ2h1rnWL_LHIvs3cV_OvRgn0SX_hfgwwXQyWjfRj0Yl564ppRrEQv3fuNaHZQ-xoV5-MUI5YSImjKyJvy8EbAUe3YQVTIOBgPWRTBZ2eD-nfUvKq-1dw</recordid><startdate>20070225</startdate><enddate>20070225</enddate><creator>Meyns, T.</creator><creator>Maes, D.</creator><creator>Calus, D.</creator><creator>Ribbens, S.</creator><creator>Dewulf, J.</creator><creator>Chiers, K.</creator><creator>de Kruif, A.</creator><creator>Cox, E.</creator><creator>Decostere, A.</creator><creator>Haesebrouck, F.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20070225</creationdate><title>Interactions of highly and low virulent Mycoplasma hyopneumoniae isolates with the respiratory tract of pigs</title><author>Meyns, T. ; Maes, D. ; Calus, D. ; Ribbens, S. ; Dewulf, J. ; Chiers, K. ; de Kruif, A. ; Cox, E. ; Decostere, A. ; Haesebrouck, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-78def7893029204237ba2d04733d7eed98ce7f39fb76d75a93d8e2dc2259a9b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>bacterial colonization</topic><topic>bacterial pneumonia</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bronchoalveolar Lavage Fluid - immunology</topic><topic>Bronchoalveolar Lavage Fluid - microbiology</topic><topic>Cytokines - metabolism</topic><topic>epidemiological studies</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>immune response</topic><topic>immunologic factors</topic><topic>in vitro studies</topic><topic>in vivo studies</topic><topic>inflammation</topic><topic>lungs</topic><topic>microbial growth</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycoplasma hyopneumoniae</topic><topic>Mycoplasma hyopneumoniae - growth & development</topic><topic>Mycoplasma hyopneumoniae - immunology</topic><topic>Mycoplasma hyopneumoniae - isolation & purification</topic><topic>Mycoplasma hyopneumoniae - pathogenicity</topic><topic>Pathogenesis</topic><topic>pathogenicity</topic><topic>physiological response</topic><topic>Pneumonia of Swine, Mycoplasmal - immunology</topic><topic>Pneumonia of Swine, Mycoplasmal - pathology</topic><topic>Pneumonia of Swine, Mycoplasmal - virology</topic><topic>Respiratory Tract Diseases - immunology</topic><topic>Respiratory Tract Diseases - pathology</topic><topic>Respiratory Tract Diseases - veterinary</topic><topic>Respiratory Tract Diseases - virology</topic><topic>strains</topic><topic>Swine</topic><topic>swine diseases</topic><topic>Time Factors</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meyns, T.</creatorcontrib><creatorcontrib>Maes, D.</creatorcontrib><creatorcontrib>Calus, D.</creatorcontrib><creatorcontrib>Ribbens, S.</creatorcontrib><creatorcontrib>Dewulf, J.</creatorcontrib><creatorcontrib>Chiers, K.</creatorcontrib><creatorcontrib>de Kruif, A.</creatorcontrib><creatorcontrib>Cox, E.</creatorcontrib><creatorcontrib>Decostere, A.</creatorcontrib><creatorcontrib>Haesebrouck, F.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meyns, T.</au><au>Maes, D.</au><au>Calus, D.</au><au>Ribbens, S.</au><au>Dewulf, J.</au><au>Chiers, K.</au><au>de Kruif, A.</au><au>Cox, E.</au><au>Decostere, A.</au><au>Haesebrouck, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interactions of highly and low virulent Mycoplasma hyopneumoniae isolates with the respiratory tract of pigs</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2007-02-25</date><risdate>2007</risdate><volume>120</volume><issue>1</issue><spage>87</spage><epage>95</epage><pages>87-95</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia, a chronic nonfatal disease affecting pigs of all ages. To obtain better insight in the mechanisms responsible for differences in virulence between highly and low virulent
M. hyopneumoniae isolates, 23 caesarean-derived, colostrum-deprived piglets were randomly assigned to three groups. Groups 1 and 2 consisted of nine animals each, which were intratracheally inoculated at 1 week of age with a highly or a low virulent isolate of
M. hyopneumoniae, respectively. The remaining five animals were inoculated with sterile culture medium. Animals were euthanized at 5, 10, 15 and 28 days post-inoculation (DPI). Animals inoculated with the highly virulent isolate had more neutrophils in BAL fluid at 10, 15 and 28
DPI compared to the other groups. At 10 and 15
DPI, animals in the highly virulent group had significantly higher concentrations of TNF-α in BAL fluid. IL-1β concentration in this group was higher at 5 and 28
DPI compared to the other groups. From 10
DPI onwards, significantly higher titres of
M. hyopneumoniae were detected in the BAL fluid of animals inoculated with the highly virulent isolate compared to animals inoculated with the low virulent isolate. Additionally, the
in vitro generation time of the highly virulent
M. hyopneumoniae isolate was significantly shorter than that of the low virulent isolate. The present study indicates that the difference in pathogenicity between the highly and low virulent isolates is associated with a faster
in vitro growth, a higher capacity to multiply in the lungs and the induction of a more severe inflammation process by the highly virulent isolate.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17123752</pmid><doi>10.1016/j.vetmic.2006.10.010</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Antibodies, Bacterial - blood bacterial colonization bacterial pneumonia Bacteriology Biological and medical sciences Bronchoalveolar Lavage Fluid - immunology Bronchoalveolar Lavage Fluid - microbiology Cytokines - metabolism epidemiological studies Fundamental and applied biological sciences. Psychology immune response immunologic factors in vitro studies in vivo studies inflammation lungs microbial growth Microbiology Miscellaneous Mycoplasma hyopneumoniae Mycoplasma hyopneumoniae - growth & development Mycoplasma hyopneumoniae - immunology Mycoplasma hyopneumoniae - isolation & purification Mycoplasma hyopneumoniae - pathogenicity Pathogenesis pathogenicity physiological response Pneumonia of Swine, Mycoplasmal - immunology Pneumonia of Swine, Mycoplasmal - pathology Pneumonia of Swine, Mycoplasmal - virology Respiratory Tract Diseases - immunology Respiratory Tract Diseases - pathology Respiratory Tract Diseases - veterinary Respiratory Tract Diseases - virology strains Swine swine diseases Time Factors Virulence |
| title | Interactions of highly and low virulent Mycoplasma hyopneumoniae isolates with the respiratory tract of pigs |
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