Assembly of trans-encapsidated recombinant viral vectors engineered from Tobacco mosaic virus and Semliki Forest virus and their evaluation as immunogens
Abstract RNA virus vectors are attractive vaccine delivery agents capable of directing high-level gene expression without integration into host cell DNA. However, delivery of non-encapsidated RNA viral vectors into animal cells is relatively inefficient. By introducing the tobacco mosaic virus (TMV)...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2007-02, Vol.358 (2), p.321-333 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Smith, Mark L Corbo, Tina Bernales, Jacqueline Lindbo, John A Pogue, Gregory P Palmer, Kenneth E McCormick, Alison A |
| description | Abstract RNA virus vectors are attractive vaccine delivery agents capable of directing high-level gene expression without integration into host cell DNA. However, delivery of non-encapsidated RNA viral vectors into animal cells is relatively inefficient. By introducing the tobacco mosaic virus (TMV) origin of assembly into the RNA genome of Semliki Forest virus (SFV), we generated an SFV expression vector that could be efficiently packaged (trans-encapsidated) in vitro by purified TMV coat protein (CP). Using cellular assays, pseudovirus disassembly, RNA replication and reporter gene expression were demonstrated. We also evaluated the immune response to trans-encapsidated recombinant SFV carrying a model antigen gene (β-galactosidase) in C57/B6 mice. Relative to RNA alone, vector encapsidation significantly improved the humoral and cellular immune responses. Furthermore, reassembly with recombinant TMV CPs permitted the display of peptide epitopes on the capsid surface as either genetic fusions or through chemical conjugation, to complement the immunoreactivity of the encapsidated RNA genetic payload. The SFV vector/TMV CP system described provides an alternative nucleic acid delivery mechanism that is safe, easy to manufacture in vitro and that also facilitates the generation of unique nucleic acid/protein antigen compositions. |
| doi_str_mv | 10.1016/j.virol.2006.08.040 |
| format | Article |
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However, delivery of non-encapsidated RNA viral vectors into animal cells is relatively inefficient. By introducing the tobacco mosaic virus (TMV) origin of assembly into the RNA genome of Semliki Forest virus (SFV), we generated an SFV expression vector that could be efficiently packaged (trans-encapsidated) in vitro by purified TMV coat protein (CP). Using cellular assays, pseudovirus disassembly, RNA replication and reporter gene expression were demonstrated. We also evaluated the immune response to trans-encapsidated recombinant SFV carrying a model antigen gene (β-galactosidase) in C57/B6 mice. Relative to RNA alone, vector encapsidation significantly improved the humoral and cellular immune responses. Furthermore, reassembly with recombinant TMV CPs permitted the display of peptide epitopes on the capsid surface as either genetic fusions or through chemical conjugation, to complement the immunoreactivity of the encapsidated RNA genetic payload. The SFV vector/TMV CP system described provides an alternative nucleic acid delivery mechanism that is safe, easy to manufacture in vitro and that also facilitates the generation of unique nucleic acid/protein antigen compositions.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2006.08.040</identifier><identifier>PMID: 17014881</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alphavirus ; Animals ; Antibodies, Viral - blood ; Antibody response ; antigens ; beta-Galactosidase - immunology ; beta-Galactosidase - metabolism ; Capsid Proteins - metabolism ; Cellular immune response ; Female ; genetic recombination ; genetic vectors ; Genetic Vectors - metabolism ; Immunization ; Immunization Schedule ; Infectious Disease ; Injections, Subcutaneous ; Lymphocyte Activation ; Mice ; Mice, Inbred C57BL ; plant viruses ; Pseudovirus ; Reassembly ; RNA, Viral - metabolism ; Semliki Forest virus ; Semliki forest virus - genetics ; Semliki forest virus - metabolism ; Spleen - immunology ; T-Lymphocytes - immunology ; Tobacco mosaic virus ; Tobacco Mosaic Virus - genetics ; Tobacco Mosaic Virus - physiology ; Viral Proteins - immunology ; Viral Proteins - metabolism ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology ; virion ; virus assembly ; Virus Replication</subject><ispartof>Virology (New York, N.Y.), 2007-02, Vol.358 (2), p.321-333</ispartof><rights>Elsevier Inc.</rights><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-58561ecedf3fc25d2dd3db3e8645e1ce3e766b19d5d01e8080fd40f2e8381cb63</citedby><cites>FETCH-LOGICAL-c512t-58561ecedf3fc25d2dd3db3e8645e1ce3e766b19d5d01e8080fd40f2e8381cb63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virol.2006.08.040$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17014881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Mark L</creatorcontrib><creatorcontrib>Corbo, Tina</creatorcontrib><creatorcontrib>Bernales, Jacqueline</creatorcontrib><creatorcontrib>Lindbo, John A</creatorcontrib><creatorcontrib>Pogue, Gregory P</creatorcontrib><creatorcontrib>Palmer, Kenneth E</creatorcontrib><creatorcontrib>McCormick, Alison A</creatorcontrib><title>Assembly of trans-encapsidated recombinant viral vectors engineered from Tobacco mosaic virus and Semliki Forest virus and their evaluation as immunogens</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Abstract RNA virus vectors are attractive vaccine delivery agents capable of directing high-level gene expression without integration into host cell DNA. However, delivery of non-encapsidated RNA viral vectors into animal cells is relatively inefficient. By introducing the tobacco mosaic virus (TMV) origin of assembly into the RNA genome of Semliki Forest virus (SFV), we generated an SFV expression vector that could be efficiently packaged (trans-encapsidated) in vitro by purified TMV coat protein (CP). Using cellular assays, pseudovirus disassembly, RNA replication and reporter gene expression were demonstrated. We also evaluated the immune response to trans-encapsidated recombinant SFV carrying a model antigen gene (β-galactosidase) in C57/B6 mice. Relative to RNA alone, vector encapsidation significantly improved the humoral and cellular immune responses. Furthermore, reassembly with recombinant TMV CPs permitted the display of peptide epitopes on the capsid surface as either genetic fusions or through chemical conjugation, to complement the immunoreactivity of the encapsidated RNA genetic payload. The SFV vector/TMV CP system described provides an alternative nucleic acid delivery mechanism that is safe, easy to manufacture in vitro and that also facilitates the generation of unique nucleic acid/protein antigen compositions.</description><subject>Alphavirus</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Antibody response</subject><subject>antigens</subject><subject>beta-Galactosidase - immunology</subject><subject>beta-Galactosidase - metabolism</subject><subject>Capsid Proteins - metabolism</subject><subject>Cellular immune response</subject><subject>Female</subject><subject>genetic recombination</subject><subject>genetic vectors</subject><subject>Genetic Vectors - metabolism</subject><subject>Immunization</subject><subject>Immunization Schedule</subject><subject>Infectious Disease</subject><subject>Injections, Subcutaneous</subject><subject>Lymphocyte Activation</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>plant viruses</subject><subject>Pseudovirus</subject><subject>Reassembly</subject><subject>RNA, Viral - metabolism</subject><subject>Semliki Forest virus</subject><subject>Semliki forest virus - genetics</subject><subject>Semliki forest virus - metabolism</subject><subject>Spleen - immunology</subject><subject>T-Lymphocytes - immunology</subject><subject>Tobacco mosaic virus</subject><subject>Tobacco Mosaic Virus - genetics</subject><subject>Tobacco Mosaic Virus - physiology</subject><subject>Viral Proteins - immunology</subject><subject>Viral Proteins - metabolism</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><subject>virion</subject><subject>virus assembly</subject><subject>Virus Replication</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2O0zAQgCMEYsvCEyCBT9xSxk7iugeQVisWkFbi0F2Jm-XYk-JuYhdPUqmPwtvi0EogLnuyRv7mR_NNUbzmsOTA5fvd8uBT7JcCQC5BLaGGJ8WCw1qWUNX8abEAqEUplRAXxQuiHeR4tYLnxQVfAa-V4ovi1xURDm1_ZLFjYzKBSgzW7Mk7M6JjCW0cWh9MGFluZ3p2QDvGRAzD1gfElKEuxYHdxdZYG9kQyXg7wxMxExzb4ND7B89uYkIa__kYf6BPDA-mn8zoY2CGmB-GKcQtBnpZPOtMT_jq_F4W9zef7q6_lLffPn-9vrotbcPFWDaqkRwtuq7qrGiccK5ybYVK1g1yixWupGz52jUOOCpQ0LkaOoGqUty2sros3p3q7lP8OeUJ9eDJYt-bgHEiLdW6yWtbPwrydZNbSZHB6gTaFIkSdnqf_GDSUXPQszq903_U6VmdBqWzupz15lx-agd0f3POrjLw9gR0JmqzTZ70_UYArwBWSnCYG384EZj3dfCYNFmfdaLz2eOoXfSPjPDxv3zb--Ct6R_wiLSLUwpZheaahAa9me9rPi-QuQgX36vfmk3Nsg</recordid><startdate>20070220</startdate><enddate>20070220</enddate><creator>Smith, Mark L</creator><creator>Corbo, Tina</creator><creator>Bernales, Jacqueline</creator><creator>Lindbo, John A</creator><creator>Pogue, Gregory P</creator><creator>Palmer, Kenneth E</creator><creator>McCormick, Alison A</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070220</creationdate><title>Assembly of trans-encapsidated recombinant viral vectors engineered from Tobacco mosaic virus and Semliki Forest virus and their evaluation as immunogens</title><author>Smith, Mark L ; Corbo, Tina ; Bernales, Jacqueline ; Lindbo, John A ; Pogue, Gregory P ; Palmer, Kenneth E ; McCormick, Alison A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-58561ecedf3fc25d2dd3db3e8645e1ce3e766b19d5d01e8080fd40f2e8381cb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Alphavirus</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Antibody response</topic><topic>antigens</topic><topic>beta-Galactosidase - immunology</topic><topic>beta-Galactosidase - metabolism</topic><topic>Capsid Proteins - metabolism</topic><topic>Cellular immune response</topic><topic>Female</topic><topic>genetic recombination</topic><topic>genetic vectors</topic><topic>Genetic Vectors - metabolism</topic><topic>Immunization</topic><topic>Immunization Schedule</topic><topic>Infectious Disease</topic><topic>Injections, Subcutaneous</topic><topic>Lymphocyte Activation</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>plant viruses</topic><topic>Pseudovirus</topic><topic>Reassembly</topic><topic>RNA, Viral - metabolism</topic><topic>Semliki Forest virus</topic><topic>Semliki forest virus - genetics</topic><topic>Semliki forest virus - metabolism</topic><topic>Spleen - immunology</topic><topic>T-Lymphocytes - immunology</topic><topic>Tobacco mosaic virus</topic><topic>Tobacco Mosaic Virus - genetics</topic><topic>Tobacco Mosaic Virus - physiology</topic><topic>Viral Proteins - immunology</topic><topic>Viral Proteins - metabolism</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - immunology</topic><topic>virion</topic><topic>virus assembly</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Mark L</creatorcontrib><creatorcontrib>Corbo, Tina</creatorcontrib><creatorcontrib>Bernales, Jacqueline</creatorcontrib><creatorcontrib>Lindbo, John A</creatorcontrib><creatorcontrib>Pogue, Gregory P</creatorcontrib><creatorcontrib>Palmer, Kenneth E</creatorcontrib><creatorcontrib>McCormick, Alison A</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Mark L</au><au>Corbo, Tina</au><au>Bernales, Jacqueline</au><au>Lindbo, John A</au><au>Pogue, Gregory P</au><au>Palmer, Kenneth E</au><au>McCormick, Alison A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assembly of trans-encapsidated recombinant viral vectors engineered from Tobacco mosaic virus and Semliki Forest virus and their evaluation as immunogens</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2007-02-20</date><risdate>2007</risdate><volume>358</volume><issue>2</issue><spage>321</spage><epage>333</epage><pages>321-333</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Abstract RNA virus vectors are attractive vaccine delivery agents capable of directing high-level gene expression without integration into host cell DNA. However, delivery of non-encapsidated RNA viral vectors into animal cells is relatively inefficient. By introducing the tobacco mosaic virus (TMV) origin of assembly into the RNA genome of Semliki Forest virus (SFV), we generated an SFV expression vector that could be efficiently packaged (trans-encapsidated) in vitro by purified TMV coat protein (CP). Using cellular assays, pseudovirus disassembly, RNA replication and reporter gene expression were demonstrated. We also evaluated the immune response to trans-encapsidated recombinant SFV carrying a model antigen gene (β-galactosidase) in C57/B6 mice. Relative to RNA alone, vector encapsidation significantly improved the humoral and cellular immune responses. Furthermore, reassembly with recombinant TMV CPs permitted the display of peptide epitopes on the capsid surface as either genetic fusions or through chemical conjugation, to complement the immunoreactivity of the encapsidated RNA genetic payload. The SFV vector/TMV CP system described provides an alternative nucleic acid delivery mechanism that is safe, easy to manufacture in vitro and that also facilitates the generation of unique nucleic acid/protein antigen compositions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17014881</pmid><doi>10.1016/j.virol.2006.08.040</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Alphavirus Animals Antibodies, Viral - blood Antibody response antigens beta-Galactosidase - immunology beta-Galactosidase - metabolism Capsid Proteins - metabolism Cellular immune response Female genetic recombination genetic vectors Genetic Vectors - metabolism Immunization Immunization Schedule Infectious Disease Injections, Subcutaneous Lymphocyte Activation Mice Mice, Inbred C57BL plant viruses Pseudovirus Reassembly RNA, Viral - metabolism Semliki Forest virus Semliki forest virus - genetics Semliki forest virus - metabolism Spleen - immunology T-Lymphocytes - immunology Tobacco mosaic virus Tobacco Mosaic Virus - genetics Tobacco Mosaic Virus - physiology Viral Proteins - immunology Viral Proteins - metabolism Viral Vaccines - administration & dosage Viral Vaccines - immunology virion virus assembly Virus Replication |
| title | Assembly of trans-encapsidated recombinant viral vectors engineered from Tobacco mosaic virus and Semliki Forest virus and their evaluation as immunogens |
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