Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients
Malassezia furfur is lypophilic yeast commonly associate with dermatological disorders. In the present work, we described the isolation of 47 M. furfur strains from three groups of patients: pityriasis versicolor (21 isolates), seborrhoeic dermatitis (15 isolates) and seborrhoeic dermatitis of the H...
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| Veröffentlicht in: | Mycopathologia (1975) 2006-10, Vol.162 (4), p.273-280 |
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| container_title | Mycopathologia (1975) |
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| creator | Gandra, Rinaldo F Simão, Rita C. G Matsumoto, Flávia E Silva, Bosco C. M. da Ruiz, Luciana S Silva, Eriques G. da Gambale, Walderez Paula, Claudete R |
| description | Malassezia furfur is lypophilic yeast commonly associate with dermatological disorders. In the present work, we described the isolation of 47 M. furfur strains from three groups of patients: pityriasis versicolor (21 isolates), seborrhoeic dermatitis (15 isolates) and seborrhoeic dermatitis of the HIV positive patients (11 isolates). To investigate the identity of the strains at molecular level, DNA genomic of M. furfur strains were prepared and used to RAPD-PCR analyses. RAPD assay were carried out using two decamer primers and bands pattern generated were analyzed by an Unweighted Pair-Group Method (UPGMA). Dendrogram established a distinct differentiation between M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation of skin disease causing by M. furfur. |
| doi_str_mv | 10.1007/s11046-006-0063-4 |
| format | Article |
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Dendrogram established a distinct differentiation between M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation of skin disease causing by M. furfur.</description><identifier>ISSN: 0301-486X</identifier><identifier>EISSN: 1573-0832</identifier><identifier>DOI: 10.1007/s11046-006-0063-4</identifier><identifier>PMID: 17039273</identifier><language>eng</language><publisher>Netherlands: Dordrecht : Kluwer Academic Publishers</publisher><subject>Acquired immune deficiency syndrome ; Acquired Immunodeficiency Syndrome - microbiology ; AIDS ; Dermatitis ; Dermatitis, Seborrheic - microbiology ; Dermatitis, Seborrheic - virology ; DNA, Fungal - genetics ; Genotype ; HIV ; Human immunodeficiency virus ; Humans ; Infections ; Investigations ; Malassezia - genetics ; Malassezia - isolation & purification ; Malassezia furfur ; Methods ; Polymerase chain reaction ; Random Amplified Polymorphic DNA Technique - methods ; Skin ; Tinea Versicolor - microbiology ; Tinea Versicolor - virology ; Yeast</subject><ispartof>Mycopathologia (1975), 2006-10, Vol.162 (4), p.273-280</ispartof><rights>Springer 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-a0fa10ebb20239c1db2ade710dd28b5c6b494c34280e12599a914e20dec3fa473</citedby><cites>FETCH-LOGICAL-c381t-a0fa10ebb20239c1db2ade710dd28b5c6b494c34280e12599a914e20dec3fa473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17039273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gandra, Rinaldo F</creatorcontrib><creatorcontrib>Simão, Rita C. 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To investigate the identity of the strains at molecular level, DNA genomic of M. furfur strains were prepared and used to RAPD-PCR analyses. RAPD assay were carried out using two decamer primers and bands pattern generated were analyzed by an Unweighted Pair-Group Method (UPGMA). Dendrogram established a distinct differentiation between M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation of skin disease causing by M. furfur.</description><subject>Acquired immune deficiency syndrome</subject><subject>Acquired Immunodeficiency Syndrome - microbiology</subject><subject>AIDS</subject><subject>Dermatitis</subject><subject>Dermatitis, Seborrheic - microbiology</subject><subject>Dermatitis, Seborrheic - virology</subject><subject>DNA, Fungal - genetics</subject><subject>Genotype</subject><subject>HIV</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Infections</subject><subject>Investigations</subject><subject>Malassezia - genetics</subject><subject>Malassezia - isolation & purification</subject><subject>Malassezia furfur</subject><subject>Methods</subject><subject>Polymerase chain reaction</subject><subject>Random Amplified Polymorphic DNA Technique - methods</subject><subject>Skin</subject><subject>Tinea Versicolor - microbiology</subject><subject>Tinea Versicolor - virology</subject><subject>Yeast</subject><issn>0301-486X</issn><issn>1573-0832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkcFu1DAQhi0EotvCA3ABiwO3wIztOPGxWqAgFVEVKnGznMQprpJ48SRIgZevl10JiQvSjOby_f9hPsaeIbxGgOoNIYLSBcCflYV6wDZYVrKAWoqHbAMSsFC1_nbCTonuAHIKq8fsBCuQRlRyw35f-CnO6y5Mt7xZ-fX51dvianvN3eSGlTzx2PNPbnBE_ldwvF9SHk5zcmEi3qc48l2Y1xQcBeI_faLQxiGmXNBx8k1M6Xv0oeWdT6Obw5ypXb5-mukJe9S7gfzT4z1jN-_ffd1-KC4_X3zcnl8WraxxLhz0DsE3jQAhTYtdI1znK4SuE3VTtrpRRrVSiRo8itIYZ1B5AZ1vZe9UJc_Yq0PvLsUfi6fZjoFaPwxu8nEhq2tTqv1L_geiKbWpdZnBl_-Ad3FJ-WVkhUCUGrXJEB6gNkWi5Hu7S2F0abUIdu_PHvzZ7G6_0qqceX4sXprRd38TR2EZeHEAehetu02B7M0XAZhNI9Rag7wHHY-gjQ</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Gandra, Rinaldo F</creator><creator>Simão, Rita C. 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| subjects | Acquired immune deficiency syndrome Acquired Immunodeficiency Syndrome - microbiology AIDS Dermatitis Dermatitis, Seborrheic - microbiology Dermatitis, Seborrheic - virology DNA, Fungal - genetics Genotype HIV Human immunodeficiency virus Humans Infections Investigations Malassezia - genetics Malassezia - isolation & purification Malassezia furfur Methods Polymerase chain reaction Random Amplified Polymorphic DNA Technique - methods Skin Tinea Versicolor - microbiology Tinea Versicolor - virology Yeast |
| title | Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients |
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