Hybridisation of surface-immobilised single-stranded oligonucleotides and polymer monitored by surface plasmon resonance

We have investigated the hybridisation of thiol-modified single-stranded DNA embedded in a polyacrylamide layer through the technique of surface plasmon resonance (SPR). Kinetic studies were carried out by two different immobilisation methods: (a) SH-ssDNA was firstly attached on gold and the remain...

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Veröffentlicht in:	Biosensors & bioelectronics 2007-02, Vol.22 (7), p.1323-1329
Hauptverfasser:	Vikholm-Lundin, Inger, Piskonen, Reetta, Albers, Willem M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylic Resins Biological and medical sciences Biotechnology DNA, Single-Stranded Fundamental and applied biological sciences. Psychology Gold Hybridisation Non-specific binding Oligodeoxyribonucleotides Oligonucleotides Polymer Repellent SH-ssDNA Surface Plasmon Resonance
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