Imaging of Germinal Center Selection Events During Affinity Maturation
The germinal center (GC) is an important site for the generation and selection of B cells bearing high-affinity antibodies, yet GC cell migration and interaction dynamics have not been directly observed. Using two-photon microscopy of mouse lymph nodes, we revealed that GC B cells are highly motile...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2007-01, Vol.315 (5811), p.528-531 |
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| creator | Allen, Christopher D.C Okada, Takaharu Tang, H. Lucy Cyster, Jason G |
| description | The germinal center (GC) is an important site for the generation and selection of B cells bearing high-affinity antibodies, yet GC cell migration and interaction dynamics have not been directly observed. Using two-photon microscopy of mouse lymph nodes, we revealed that GC B cells are highly motile and extend long cell processes. They transited between GC dark and light zones and divided in both regions, although these B cells resided for only several hours in the light zone where antigen is displayed. GC B cells formed few stable contacts with GC T cells despite frequent encounters, and T cells were seen to carry dead B cell blebs. On the basis of these observations, we propose a model in which competition for T cell help plays a more dominant role in the selection of GC B cells than previously appreciated. |
| doi_str_mv | 10.1126/science.1136736 |
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Lucy ; Cyster, Jason G</creator><creatorcontrib>Allen, Christopher D.C ; Okada, Takaharu ; Tang, H. Lucy ; Cyster, Jason G</creatorcontrib><description>The germinal center (GC) is an important site for the generation and selection of B cells bearing high-affinity antibodies, yet GC cell migration and interaction dynamics have not been directly observed. Using two-photon microscopy of mouse lymph nodes, we revealed that GC B cells are highly motile and extend long cell processes. They transited between GC dark and light zones and divided in both regions, although these B cells resided for only several hours in the light zone where antigen is displayed. GC B cells formed few stable contacts with GC T cells despite frequent encounters, and T cells were seen to carry dead B cell blebs. On the basis of these observations, we propose a model in which competition for T cell help plays a more dominant role in the selection of GC B cells than previously appreciated.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1136736</identifier><identifier>PMID: 17185562</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Animals ; Antibodies ; Antibody Affinity ; Antigens ; B lymphocytes ; B-Lymphocytes - cytology ; B-Lymphocytes - immunology ; B-Lymphocytes - physiology ; Biological and medical sciences ; Cell adhesion & migration ; Cell Cycle ; Cell Death ; Cell lines ; Cell Movement ; Cellular biology ; Dendritic Cells, Follicular - cytology ; Dendritic Cells, Follicular - physiology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Germinal Center - cytology ; Germinal Center - immunology ; Globular star clusters ; Immune system ; Immunobiology ; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation ; Lymphoid tissue ; Macrophages - physiology ; Mice ; Mice, Inbred C57BL ; Microscopy - methods ; Models, Immunological ; Mutation ; Plasma cells ; rev genes ; Rodents ; Scientific imaging ; T lymphocytes ; T-Lymphocytes - cytology ; T-Lymphocytes - immunology ; T-Lymphocytes - physiology</subject><ispartof>Science (American Association for the Advancement of Science), 2007-01, Vol.315 (5811), p.528-531</ispartof><rights>Copyright 2007 American Association for the Advancement of Science</rights><rights>2007 INIST-CNRS</rights><rights>Copyright American Association for the Advancement of Science Jan 26, 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c597t-6269566309f096499e616dbe5e2eb89156f5b0ffcb96d35231e7833c42cb2c103</citedby><cites>FETCH-LOGICAL-c597t-6269566309f096499e616dbe5e2eb89156f5b0ffcb96d35231e7833c42cb2c103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20038845$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20038845$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18505609$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17185562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Allen, Christopher D.C</creatorcontrib><creatorcontrib>Okada, Takaharu</creatorcontrib><creatorcontrib>Tang, H. Lucy</creatorcontrib><creatorcontrib>Cyster, Jason G</creatorcontrib><title>Imaging of Germinal Center Selection Events During Affinity Maturation</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The germinal center (GC) is an important site for the generation and selection of B cells bearing high-affinity antibodies, yet GC cell migration and interaction dynamics have not been directly observed. Using two-photon microscopy of mouse lymph nodes, we revealed that GC B cells are highly motile and extend long cell processes. They transited between GC dark and light zones and divided in both regions, although these B cells resided for only several hours in the light zone where antigen is displayed. GC B cells formed few stable contacts with GC T cells despite frequent encounters, and T cells were seen to carry dead B cell blebs. On the basis of these observations, we propose a model in which competition for T cell help plays a more dominant role in the selection of GC B cells than previously appreciated.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibody Affinity</subject><subject>Antigens</subject><subject>B lymphocytes</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - physiology</subject><subject>Biological and medical sciences</subject><subject>Cell adhesion & migration</subject><subject>Cell Cycle</subject><subject>Cell Death</subject><subject>Cell lines</subject><subject>Cell Movement</subject><subject>Cellular biology</subject><subject>Dendritic Cells, Follicular - cytology</subject><subject>Dendritic Cells, Follicular - physiology</subject><subject>Fundamental and applied biological sciences. 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Lucy</au><au>Cyster, Jason G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imaging of Germinal Center Selection Events During Affinity Maturation</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2007-01-26</date><risdate>2007</risdate><volume>315</volume><issue>5811</issue><spage>528</spage><epage>531</epage><pages>528-531</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>The germinal center (GC) is an important site for the generation and selection of B cells bearing high-affinity antibodies, yet GC cell migration and interaction dynamics have not been directly observed. Using two-photon microscopy of mouse lymph nodes, we revealed that GC B cells are highly motile and extend long cell processes. They transited between GC dark and light zones and divided in both regions, although these B cells resided for only several hours in the light zone where antigen is displayed. GC B cells formed few stable contacts with GC T cells despite frequent encounters, and T cells were seen to carry dead B cell blebs. On the basis of these observations, we propose a model in which competition for T cell help plays a more dominant role in the selection of GC B cells than previously appreciated.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>17185562</pmid><doi>10.1126/science.1136736</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Animals Antibodies Antibody Affinity Antigens B lymphocytes B-Lymphocytes - cytology B-Lymphocytes - immunology B-Lymphocytes - physiology Biological and medical sciences Cell adhesion & migration Cell Cycle Cell Death Cell lines Cell Movement Cellular biology Dendritic Cells, Follicular - cytology Dendritic Cells, Follicular - physiology Fundamental and applied biological sciences. Psychology Fundamental immunology Germinal Center - cytology Germinal Center - immunology Globular star clusters Immune system Immunobiology Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation Lymphoid tissue Macrophages - physiology Mice Mice, Inbred C57BL Microscopy - methods Models, Immunological Mutation Plasma cells rev genes Rodents Scientific imaging T lymphocytes T-Lymphocytes - cytology T-Lymphocytes - immunology T-Lymphocytes - physiology |
| title | Imaging of Germinal Center Selection Events During Affinity Maturation |
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