P2X4 is Up-regulated in Gingival Fibroblasts after Periodontal Surgery
Several studies have shown that surgical detachment of marginal gingiva close to the cervical cementum of molar teeth in a rat mandible is a distinct stimulus for alveolar bone resorption. Recently, we found that P2X4, an ATP-receptor, is significantly up-regulated in marginal gingival cells soon af...
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| Veröffentlicht in: | Journal of dental research 2007-02, Vol.86 (2), p.181-185 |
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| creator | Binderman, I. Bahar, H. Jacob-Hirsch, J. Zeligson, S. Amariglio, N. Rechavi, G. Shoham, S. Yaffe, A. |
| description | Several studies have shown that surgical detachment of marginal gingiva close to the cervical cementum of molar teeth in a rat mandible is a distinct stimulus for alveolar bone resorption. Recently, we found that P2X4, an ATP-receptor, is significantly up-regulated in marginal gingival cells soon after surgery. We hypothesized that local release of ATP signaling through P2X4 elicits activation of osteoclasts on the alveolar bone surface. In this study, we identified intense immunoreactivity of gingival fibroblasts to P2X4-specific antibodies and a 6.4-fold increase in expression by real-time RT-PCR. Moreover, a single local application, at the time of surgery, of Apyrase (which degrades ATP) or Coomassie Brilliant Blue (an antagonist of purinoreceptors) significantly reduced alveolar bone loss. We propose that ATP flowing from cells after surgery can directly activate P2X4 receptors in the sensor cells of marginal gingiva through Ca2+ signaling, or by direct activation of osteoclasts on the bone surface. |
| doi_str_mv | 10.1177/154405910708600214 |
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Recently, we found that P2X4, an ATP-receptor, is significantly up-regulated in marginal gingival cells soon after surgery. We hypothesized that local release of ATP signaling through P2X4 elicits activation of osteoclasts on the alveolar bone surface. In this study, we identified intense immunoreactivity of gingival fibroblasts to P2X4-specific antibodies and a 6.4-fold increase in expression by real-time RT-PCR. Moreover, a single local application, at the time of surgery, of Apyrase (which degrades ATP) or Coomassie Brilliant Blue (an antagonist of purinoreceptors) significantly reduced alveolar bone loss. We propose that ATP flowing from cells after surgery can directly activate P2X4 receptors in the sensor cells of marginal gingiva through Ca2+ signaling, or by direct activation of osteoclasts on the bone surface.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/154405910708600214</identifier><identifier>PMID: 17251520</identifier><identifier>CODEN: JDREAF</identifier><language>eng</language><publisher>United States: SAGE Publications</publisher><subject>Adenosine Triphosphate - antagonists & inhibitors ; Adenosine Triphosphate - physiology ; Alveolar Bone Loss - etiology ; Alveolar Bone Loss - metabolism ; Alveolar Bone Loss - prevention & control ; Analysis of Variance ; Animals ; Apyrase - physiology ; Dentistry ; Fibroblasts - metabolism ; Gingiva - cytology ; Gingiva - metabolism ; Gingivectomy - adverse effects ; Indicators and Reagents - pharmacology ; Osteoclasts - drug effects ; Rats ; Rats, Wistar ; Receptors, Purinergic P2 - biosynthesis ; Receptors, Purinergic P2X4 ; Reverse Transcriptase Polymerase Chain Reaction ; Rosaniline Dyes - pharmacology ; Up-Regulation</subject><ispartof>Journal of dental research, 2007-02, Vol.86 (2), p.181-185</ispartof><rights>International and American Associations for Dental Research</rights><rights>Copyright American Association for Dental Research/American Academy of Implant Dentistry Feb 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-795c30b3f8e1e74e0290b5696e1351a36e9ca125c045e4631aa5091aed51c3dd3</citedby><cites>FETCH-LOGICAL-c397t-795c30b3f8e1e74e0290b5696e1351a36e9ca125c045e4631aa5091aed51c3dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/154405910708600214$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/154405910708600214$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21817,27922,27923,43619,43620</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17251520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Binderman, I.</creatorcontrib><creatorcontrib>Bahar, H.</creatorcontrib><creatorcontrib>Jacob-Hirsch, J.</creatorcontrib><creatorcontrib>Zeligson, S.</creatorcontrib><creatorcontrib>Amariglio, N.</creatorcontrib><creatorcontrib>Rechavi, G.</creatorcontrib><creatorcontrib>Shoham, S.</creatorcontrib><creatorcontrib>Yaffe, A.</creatorcontrib><title>P2X4 is Up-regulated in Gingival Fibroblasts after Periodontal Surgery</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Several studies have shown that surgical detachment of marginal gingiva close to the cervical cementum of molar teeth in a rat mandible is a distinct stimulus for alveolar bone resorption. Recently, we found that P2X4, an ATP-receptor, is significantly up-regulated in marginal gingival cells soon after surgery. We hypothesized that local release of ATP signaling through P2X4 elicits activation of osteoclasts on the alveolar bone surface. In this study, we identified intense immunoreactivity of gingival fibroblasts to P2X4-specific antibodies and a 6.4-fold increase in expression by real-time RT-PCR. Moreover, a single local application, at the time of surgery, of Apyrase (which degrades ATP) or Coomassie Brilliant Blue (an antagonist of purinoreceptors) significantly reduced alveolar bone loss. We propose that ATP flowing from cells after surgery can directly activate P2X4 receptors in the sensor cells of marginal gingiva through Ca2+ signaling, or by direct activation of osteoclasts on the bone surface.</description><subject>Adenosine Triphosphate - antagonists & inhibitors</subject><subject>Adenosine Triphosphate - physiology</subject><subject>Alveolar Bone Loss - etiology</subject><subject>Alveolar Bone Loss - metabolism</subject><subject>Alveolar Bone Loss - prevention & control</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Apyrase - physiology</subject><subject>Dentistry</subject><subject>Fibroblasts - metabolism</subject><subject>Gingiva - cytology</subject><subject>Gingiva - metabolism</subject><subject>Gingivectomy - adverse effects</subject><subject>Indicators and Reagents - pharmacology</subject><subject>Osteoclasts - drug effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Purinergic P2 - biosynthesis</subject><subject>Receptors, Purinergic P2X4</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rosaniline Dyes - pharmacology</subject><subject>Up-Regulation</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE9Lw0AQxRdRbK1-AQ8SPHiLndk_SfcoxVahYEEL3sImmYSUNKm7idBv75YWCgpeduHt772ZfYzdIjwixvEYlZSgNEIMkwiAozxjw70Y7tVzNvQaD0FINWBXzq0BUPOJuGQDjLlCxWHIZkv-KYPKBattaKnsa9NRHlRNMK-asvo2dTCrUtumtXGdC0zRkQ2WZKs2b5vOv773tiS7u2YXhakd3RzvEVvNnj-mL-Hibf46fVqEmdBxF8ZaZQJSUUwIKZYEXEOqIh0RCoVGRKQzg1xlIBXJSKAxCjQayhVmIs_FiD0ccre2_erJdcmmchnVtWmo7V0STbSM_N88eP8LXLe9bfxuCQctlfSHh_gBymzrnKUi2dpqY-wuQUj2FSd_K_amu2Nyn24oP1mOnXpgfACcKek09p_IH8yKgS8</recordid><startdate>200702</startdate><enddate>200702</enddate><creator>Binderman, I.</creator><creator>Bahar, H.</creator><creator>Jacob-Hirsch, J.</creator><creator>Zeligson, S.</creator><creator>Amariglio, N.</creator><creator>Rechavi, G.</creator><creator>Shoham, S.</creator><creator>Yaffe, A.</creator><general>SAGE Publications</general><general>SAGE PUBLICATIONS, INC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>200702</creationdate><title>P2X4 is Up-regulated in Gingival Fibroblasts after Periodontal Surgery</title><author>Binderman, I. ; Bahar, H. ; Jacob-Hirsch, J. ; Zeligson, S. ; Amariglio, N. ; Rechavi, G. ; Shoham, S. ; Yaffe, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-795c30b3f8e1e74e0290b5696e1351a36e9ca125c045e4631aa5091aed51c3dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenosine Triphosphate - antagonists & inhibitors</topic><topic>Adenosine Triphosphate - physiology</topic><topic>Alveolar Bone Loss - etiology</topic><topic>Alveolar Bone Loss - metabolism</topic><topic>Alveolar Bone Loss - prevention & control</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Apyrase - physiology</topic><topic>Dentistry</topic><topic>Fibroblasts - metabolism</topic><topic>Gingiva - cytology</topic><topic>Gingiva - metabolism</topic><topic>Gingivectomy - adverse effects</topic><topic>Indicators and Reagents - pharmacology</topic><topic>Osteoclasts - drug effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Purinergic P2 - biosynthesis</topic><topic>Receptors, Purinergic P2X4</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rosaniline Dyes - pharmacology</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Binderman, I.</creatorcontrib><creatorcontrib>Bahar, H.</creatorcontrib><creatorcontrib>Jacob-Hirsch, J.</creatorcontrib><creatorcontrib>Zeligson, S.</creatorcontrib><creatorcontrib>Amariglio, N.</creatorcontrib><creatorcontrib>Rechavi, G.</creatorcontrib><creatorcontrib>Shoham, S.</creatorcontrib><creatorcontrib>Yaffe, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Binderman, I.</au><au>Bahar, H.</au><au>Jacob-Hirsch, J.</au><au>Zeligson, S.</au><au>Amariglio, N.</au><au>Rechavi, G.</au><au>Shoham, S.</au><au>Yaffe, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P2X4 is Up-regulated in Gingival Fibroblasts after Periodontal Surgery</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2007-02</date><risdate>2007</risdate><volume>86</volume><issue>2</issue><spage>181</spage><epage>185</epage><pages>181-185</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><coden>JDREAF</coden><abstract>Several studies have shown that surgical detachment of marginal gingiva close to the cervical cementum of molar teeth in a rat mandible is a distinct stimulus for alveolar bone resorption. Recently, we found that P2X4, an ATP-receptor, is significantly up-regulated in marginal gingival cells soon after surgery. We hypothesized that local release of ATP signaling through P2X4 elicits activation of osteoclasts on the alveolar bone surface. In this study, we identified intense immunoreactivity of gingival fibroblasts to P2X4-specific antibodies and a 6.4-fold increase in expression by real-time RT-PCR. Moreover, a single local application, at the time of surgery, of Apyrase (which degrades ATP) or Coomassie Brilliant Blue (an antagonist of purinoreceptors) significantly reduced alveolar bone loss. We propose that ATP flowing from cells after surgery can directly activate P2X4 receptors in the sensor cells of marginal gingiva through Ca2+ signaling, or by direct activation of osteoclasts on the bone surface.</abstract><cop>United States</cop><pub>SAGE Publications</pub><pmid>17251520</pmid><doi>10.1177/154405910708600214</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of dental research, 2007-02, Vol.86 (2), p.181-185 |
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| source | MEDLINE; SAGE Complete A-Z List |
| subjects | Adenosine Triphosphate - antagonists & inhibitors Adenosine Triphosphate - physiology Alveolar Bone Loss - etiology Alveolar Bone Loss - metabolism Alveolar Bone Loss - prevention & control Analysis of Variance Animals Apyrase - physiology Dentistry Fibroblasts - metabolism Gingiva - cytology Gingiva - metabolism Gingivectomy - adverse effects Indicators and Reagents - pharmacology Osteoclasts - drug effects Rats Rats, Wistar Receptors, Purinergic P2 - biosynthesis Receptors, Purinergic P2X4 Reverse Transcriptase Polymerase Chain Reaction Rosaniline Dyes - pharmacology Up-Regulation |
| title | P2X4 is Up-regulated in Gingival Fibroblasts after Periodontal Surgery |
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