Composition of the bacterial flora in tonsilloliths
Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individ...
Gespeichert in:
| Veröffentlicht in: | Microbes and infection 2006-08, Vol.8 (9), p.2384-2389 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2389 |
|---|---|
| container_issue | 9 |
| container_start_page | 2384 |
| container_title | Microbes and infection |
| container_volume | 8 |
| creator | Tsuneishi, Midori Yamamoto, Tatsuo Kokeguchi, Susumu Tamaki, Naofumi Fukui, Kazuhiro Watanabe, Tatsuo |
| description | Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into
Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera
Eubacterium,
Fusobacterium,
Megasphaera,
Porphyromonas,
Prevotella,
Selenomonas and
Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor. |
| doi_str_mv | 10.1016/j.micinf.2006.04.023 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68941331</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1286457906002280</els_id><sourcerecordid>19373173</sourcerecordid><originalsourceid>FETCH-LOGICAL-c533t-9a113b347d81aee6c2ea92ea2da5e12fc73d23e431c49aa75697f3ce1f2b76d03</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVJadKk_6AEX5qbXY1GlqxLoSzpBwR6aSE3oZVHRItsbSRvof8-DruQW3MYZg7P-zI8jH0E3gEH9XnXTdHHOXSCc9Vx2XGBb9gFaGVaDfL-bL3FoFrZa3PO3te64xx6reQ7dg5q6I3p-QXDTZ72ucYl5rnJoVkeqNk6v1CJLjUh5eKaODdLnmtMKae4PNQr9ja4VOnDaV-yP99uf29-tHe_vv_cfL1rfY-4tMYB4BalHgdwRMoLcmYdMbqeQASvcRRIEsFL45zuldEBPUEQW61Gjpfs5ti7L_nxQHWxU6yeUnIz5UO1ajASEOFVEAxqBI0rKI-gL7nWQsHuS5xc-WeB22erdmePVu2zVculXa2usetT_2E70fgSOmlcgU8nwFXvUihu9rG-cIMAA2hW7suRo1Xb30jFVh9p9jTGQn6xY47__-QJJUSW-w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19373173</pqid></control><display><type>article</type><title>Composition of the bacterial flora in tonsilloliths</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Tsuneishi, Midori ; Yamamoto, Tatsuo ; Kokeguchi, Susumu ; Tamaki, Naofumi ; Fukui, Kazuhiro ; Watanabe, Tatsuo</creator><creatorcontrib>Tsuneishi, Midori ; Yamamoto, Tatsuo ; Kokeguchi, Susumu ; Tamaki, Naofumi ; Fukui, Kazuhiro ; Watanabe, Tatsuo</creatorcontrib><description>Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into
Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera
Eubacterium,
Fusobacterium,
Megasphaera,
Porphyromonas,
Prevotella,
Selenomonas and
Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor.</description><identifier>ISSN: 1286-4579</identifier><identifier>EISSN: 1769-714X</identifier><identifier>DOI: 10.1016/j.micinf.2006.04.023</identifier><identifier>PMID: 16859950</identifier><language>eng</language><publisher>Lausanne: Elsevier SAS</publisher><subject>Adult ; Bacteria ; Bacteria, Anaerobic - isolation & purification ; Bacteria, Anaerobic - metabolism ; Bacteriology ; Biological and medical sciences ; Calculi - microbiology ; Escherichia coli ; Eubacterium ; Female ; Fundamental and applied biological sciences. Psychology ; Fusobacterium ; Halitosis - microbiology ; Humans ; Male ; Microbiology ; Microscopy, Electron, Scanning - methods ; Middle Aged ; Miscellaneous ; Oral malodor ; Palatine Tonsil - microbiology ; Porphyromonas ; Prevotella ; Tonsillolith ; Volatile sulfur compound</subject><ispartof>Microbes and infection, 2006-08, Vol.8 (9), p.2384-2389</ispartof><rights>2006 Elsevier SAS</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-9a113b347d81aee6c2ea92ea2da5e12fc73d23e431c49aa75697f3ce1f2b76d03</citedby><cites>FETCH-LOGICAL-c533t-9a113b347d81aee6c2ea92ea2da5e12fc73d23e431c49aa75697f3ce1f2b76d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1286457906002280$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18219139$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16859950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsuneishi, Midori</creatorcontrib><creatorcontrib>Yamamoto, Tatsuo</creatorcontrib><creatorcontrib>Kokeguchi, Susumu</creatorcontrib><creatorcontrib>Tamaki, Naofumi</creatorcontrib><creatorcontrib>Fukui, Kazuhiro</creatorcontrib><creatorcontrib>Watanabe, Tatsuo</creatorcontrib><title>Composition of the bacterial flora in tonsilloliths</title><title>Microbes and infection</title><addtitle>Microbes Infect</addtitle><description>Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into
Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera
Eubacterium,
Fusobacterium,
Megasphaera,
Porphyromonas,
Prevotella,
Selenomonas and
Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor.</description><subject>Adult</subject><subject>Bacteria</subject><subject>Bacteria, Anaerobic - isolation & purification</subject><subject>Bacteria, Anaerobic - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Calculi - microbiology</subject><subject>Escherichia coli</subject><subject>Eubacterium</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fusobacterium</subject><subject>Halitosis - microbiology</subject><subject>Humans</subject><subject>Male</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Scanning - methods</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Oral malodor</subject><subject>Palatine Tonsil - microbiology</subject><subject>Porphyromonas</subject><subject>Prevotella</subject><subject>Tonsillolith</subject><subject>Volatile sulfur compound</subject><issn>1286-4579</issn><issn>1769-714X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVJadKk_6AEX5qbXY1GlqxLoSzpBwR6aSE3oZVHRItsbSRvof8-DruQW3MYZg7P-zI8jH0E3gEH9XnXTdHHOXSCc9Vx2XGBb9gFaGVaDfL-bL3FoFrZa3PO3te64xx6reQ7dg5q6I3p-QXDTZ72ucYl5rnJoVkeqNk6v1CJLjUh5eKaODdLnmtMKae4PNQr9ja4VOnDaV-yP99uf29-tHe_vv_cfL1rfY-4tMYB4BalHgdwRMoLcmYdMbqeQASvcRRIEsFL45zuldEBPUEQW61Gjpfs5ti7L_nxQHWxU6yeUnIz5UO1ajASEOFVEAxqBI0rKI-gL7nWQsHuS5xc-WeB22erdmePVu2zVculXa2usetT_2E70fgSOmlcgU8nwFXvUihu9rG-cIMAA2hW7suRo1Xb30jFVh9p9jTGQn6xY47__-QJJUSW-w</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Tsuneishi, Midori</creator><creator>Yamamoto, Tatsuo</creator><creator>Kokeguchi, Susumu</creator><creator>Tamaki, Naofumi</creator><creator>Fukui, Kazuhiro</creator><creator>Watanabe, Tatsuo</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Composition of the bacterial flora in tonsilloliths</title><author>Tsuneishi, Midori ; Yamamoto, Tatsuo ; Kokeguchi, Susumu ; Tamaki, Naofumi ; Fukui, Kazuhiro ; Watanabe, Tatsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-9a113b347d81aee6c2ea92ea2da5e12fc73d23e431c49aa75697f3ce1f2b76d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Bacteria</topic><topic>Bacteria, Anaerobic - isolation & purification</topic><topic>Bacteria, Anaerobic - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Calculi - microbiology</topic><topic>Escherichia coli</topic><topic>Eubacterium</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fusobacterium</topic><topic>Halitosis - microbiology</topic><topic>Humans</topic><topic>Male</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Oral malodor</topic><topic>Palatine Tonsil - microbiology</topic><topic>Porphyromonas</topic><topic>Prevotella</topic><topic>Tonsillolith</topic><topic>Volatile sulfur compound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsuneishi, Midori</creatorcontrib><creatorcontrib>Yamamoto, Tatsuo</creatorcontrib><creatorcontrib>Kokeguchi, Susumu</creatorcontrib><creatorcontrib>Tamaki, Naofumi</creatorcontrib><creatorcontrib>Fukui, Kazuhiro</creatorcontrib><creatorcontrib>Watanabe, Tatsuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbes and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsuneishi, Midori</au><au>Yamamoto, Tatsuo</au><au>Kokeguchi, Susumu</au><au>Tamaki, Naofumi</au><au>Fukui, Kazuhiro</au><au>Watanabe, Tatsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Composition of the bacterial flora in tonsilloliths</atitle><jtitle>Microbes and infection</jtitle><addtitle>Microbes Infect</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>8</volume><issue>9</issue><spage>2384</spage><epage>2389</epage><pages>2384-2389</pages><issn>1286-4579</issn><eissn>1769-714X</eissn><abstract>Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into
Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera
Eubacterium,
Fusobacterium,
Megasphaera,
Porphyromonas,
Prevotella,
Selenomonas and
Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>16859950</pmid><doi>10.1016/j.micinf.2006.04.023</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1286-4579 |
| ispartof | Microbes and infection, 2006-08, Vol.8 (9), p.2384-2389 |
| issn | 1286-4579 1769-714X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68941331 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Bacteria Bacteria, Anaerobic - isolation & purification Bacteria, Anaerobic - metabolism Bacteriology Biological and medical sciences Calculi - microbiology Escherichia coli Eubacterium Female Fundamental and applied biological sciences. Psychology Fusobacterium Halitosis - microbiology Humans Male Microbiology Microscopy, Electron, Scanning - methods Middle Aged Miscellaneous Oral malodor Palatine Tonsil - microbiology Porphyromonas Prevotella Tonsillolith Volatile sulfur compound |
| title | Composition of the bacterial flora in tonsilloliths |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T20%3A54%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Composition%20of%20the%20bacterial%20flora%20in%20tonsilloliths&rft.jtitle=Microbes%20and%20infection&rft.au=Tsuneishi,%20Midori&rft.date=2006-08-01&rft.volume=8&rft.issue=9&rft.spage=2384&rft.epage=2389&rft.pages=2384-2389&rft.issn=1286-4579&rft.eissn=1769-714X&rft_id=info:doi/10.1016/j.micinf.2006.04.023&rft_dat=%3Cproquest_cross%3E19373173%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19373173&rft_id=info:pmid/16859950&rft_els_id=S1286457906002280&rfr_iscdi=true |