The influence of diabetes on gingival crevicular fluid β-glucuronidase and interleukin-8

Objectives: Polymorphonuclear neutrophil (PMN) dysfunction is associated with diabetes. We examined the gingival crevicular fluid (GCF) β‐glucuronidase (BG) and interleukin‐8 (IL‐8) levels of periodontitis patients with and without type 2 diabetes mellitus (DM). Material and methods: Forty five adults with type 2 DM and 32 adults without DM, both with chronic periodontitis were enrolled. GCF was collected from eight posterior sites in each quadrant, and periodontal parameters were recorded. GCF was assayed for IL‐8 by ELISA and BG by a flourometric assay. Results: GCF IL‐8 was positively correlated with probing depth (PD), and GCF BG but not clinical attachment level (CAL), bleeding on probing (BOP), or plaque index (PI). In contrast, GCF BG was strongly correlated with each of the clinical measures of periodontal disease. Subjects with DM significantly lower levels of both BG (73.0±44.8 versus 121.9±84.6 pg/sample; p=0.002) and IL‐8 (32.1±33.1 versus 90.8±83.2 pg/sample; p