Paradoxical antagonism of PACAP receptor signaling by VIP in Xenopus oocytes via the type-C natriuretic peptide receptor
Atrial natriuretic peptide (ANP) and the closely-related peptides BNP and CNP are highly conserved cardiovascular hormones. They bind to single transmembrane-spanning receptors, triggering receptor-intrinsic guanylyl cyclase activity. The “truncated” type-C natriuretic peptide receptor (NPR-C) has l...
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| Veröffentlicht in: | Cellular signalling 2006-11, Vol.18 (11), p.2013-2021 |
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| creator | Lelièvre, V. Hu, Z. Ioffe, Y. Byun, J.-Y. Flores, A. Seksenyan, A. Waschek, J.A. |
| description | Atrial natriuretic peptide (ANP) and the closely-related peptides BNP and CNP are highly conserved cardiovascular hormones. They bind to single transmembrane-spanning receptors, triggering receptor-intrinsic guanylyl cyclase activity. The “truncated” type-C natriuretic peptide receptor (NPR-C) has long been called a clearance receptor because it lacks the intracellular guanylyl cyclase domain, though data suggest it might negatively couple to adenylyl cyclase via G
i. Here we report the molecular cloning and characterization of the
Xenopus laevis type-C natriuretic peptide receptor (
XNPR-C). Analysis confirms the presence of a short intracellular C-terminus, as well as a high similarity to fish and mammalian NPR-C. Injection of
XNPR-C mRNA into
Xenopus oocytes resulted in expression of high affinity [
125I]ANP binding sites that were competitively and completely displaced by natriuretic analogs and the unrelated neuropeptide vasoactive intestinal peptide (VIP). Measurement of cAMP levels in mRNA-injected oocytes revealed that
XNPR-C is negatively coupled to adenylyl cyclase in a pertussis toxin-sensitive manner. When
XNPR-C was co-expressed with PAC
1 receptors for pituitary adenylyl cyclase-activating polypeptide (PACAP), VIP and natriuretic peptides counteracted the cAMP induction by PACAP. These results suggest that VIP and natriuretic peptides can potentially modulate the action of PACAP in cells where these receptors are co-expressed. |
| doi_str_mv | 10.1016/j.cellsig.2006.03.013 |
| format | Article |
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i. Here we report the molecular cloning and characterization of the
Xenopus laevis type-C natriuretic peptide receptor (
XNPR-C). Analysis confirms the presence of a short intracellular C-terminus, as well as a high similarity to fish and mammalian NPR-C. Injection of
XNPR-C mRNA into
Xenopus oocytes resulted in expression of high affinity [
125I]ANP binding sites that were competitively and completely displaced by natriuretic analogs and the unrelated neuropeptide vasoactive intestinal peptide (VIP). Measurement of cAMP levels in mRNA-injected oocytes revealed that
XNPR-C is negatively coupled to adenylyl cyclase in a pertussis toxin-sensitive manner. When
XNPR-C was co-expressed with PAC
1 receptors for pituitary adenylyl cyclase-activating polypeptide (PACAP), VIP and natriuretic peptides counteracted the cAMP induction by PACAP. These results suggest that VIP and natriuretic peptides can potentially modulate the action of PACAP in cells where these receptors are co-expressed.</description><identifier>ISSN: 0898-6568</identifier><identifier>EISSN: 1873-3913</identifier><identifier>DOI: 10.1016/j.cellsig.2006.03.013</identifier><identifier>PMID: 16723209</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Adenylyl Cyclases - metabolism ; Amino Acid Sequence ; Animals ; Binding, Competitive ; cAMP ; Cloning, Molecular ; DNA, Complementary - genetics ; Humans ; Molecular Sequence Data ; Natriuretic peptide ; Neuropeptide ; Oocytes - drug effects ; Oocytes - metabolism ; Rats ; Receptor ; Receptors, Atrial Natriuretic Factor - genetics ; Receptors, Atrial Natriuretic Factor - metabolism ; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide - antagonists & inhibitors ; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide - metabolism ; RNA, Messenger - metabolism ; RNA, Messenger - pharmacology ; Sequence Alignment ; Signal Transduction ; Signaling ; Vasoactive Intestinal Peptide - metabolism ; Vasoactive Intestinal Peptide - pharmacology ; Xenopus</subject><ispartof>Cellular signalling, 2006-11, Vol.18 (11), p.2013-2021</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-72c748e80f3ca6e752eb5176d15941358b968d30e93c284fe194def12681332a3</citedby><cites>FETCH-LOGICAL-c363t-72c748e80f3ca6e752eb5176d15941358b968d30e93c284fe194def12681332a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cellsig.2006.03.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16723209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lelièvre, V.</creatorcontrib><creatorcontrib>Hu, Z.</creatorcontrib><creatorcontrib>Ioffe, Y.</creatorcontrib><creatorcontrib>Byun, J.-Y.</creatorcontrib><creatorcontrib>Flores, A.</creatorcontrib><creatorcontrib>Seksenyan, A.</creatorcontrib><creatorcontrib>Waschek, J.A.</creatorcontrib><title>Paradoxical antagonism of PACAP receptor signaling by VIP in Xenopus oocytes via the type-C natriuretic peptide receptor</title><title>Cellular signalling</title><addtitle>Cell Signal</addtitle><description>Atrial natriuretic peptide (ANP) and the closely-related peptides BNP and CNP are highly conserved cardiovascular hormones. They bind to single transmembrane-spanning receptors, triggering receptor-intrinsic guanylyl cyclase activity. The “truncated” type-C natriuretic peptide receptor (NPR-C) has long been called a clearance receptor because it lacks the intracellular guanylyl cyclase domain, though data suggest it might negatively couple to adenylyl cyclase via G
i. Here we report the molecular cloning and characterization of the
Xenopus laevis type-C natriuretic peptide receptor (
XNPR-C). Analysis confirms the presence of a short intracellular C-terminus, as well as a high similarity to fish and mammalian NPR-C. Injection of
XNPR-C mRNA into
Xenopus oocytes resulted in expression of high affinity [
125I]ANP binding sites that were competitively and completely displaced by natriuretic analogs and the unrelated neuropeptide vasoactive intestinal peptide (VIP). Measurement of cAMP levels in mRNA-injected oocytes revealed that
XNPR-C is negatively coupled to adenylyl cyclase in a pertussis toxin-sensitive manner. When
XNPR-C was co-expressed with PAC
1 receptors for pituitary adenylyl cyclase-activating polypeptide (PACAP), VIP and natriuretic peptides counteracted the cAMP induction by PACAP. These results suggest that VIP and natriuretic peptides can potentially modulate the action of PACAP in cells where these receptors are co-expressed.</description><subject>Adenylyl Cyclases - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Binding, Competitive</subject><subject>cAMP</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Natriuretic peptide</subject><subject>Neuropeptide</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - metabolism</subject><subject>Rats</subject><subject>Receptor</subject><subject>Receptors, Atrial Natriuretic Factor - genetics</subject><subject>Receptors, Atrial Natriuretic Factor - metabolism</subject><subject>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide - antagonists & inhibitors</subject><subject>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Messenger - pharmacology</subject><subject>Sequence Alignment</subject><subject>Signal Transduction</subject><subject>Signaling</subject><subject>Vasoactive Intestinal Peptide - metabolism</subject><subject>Vasoactive Intestinal Peptide - pharmacology</subject><subject>Xenopus</subject><issn>0898-6568</issn><issn>1873-3913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv0zAYhi00xMrgJwz5tFuC7S9xnBOqqgGTJtEDTLtZrvOlc5XawXam9t-TqhUcOX2X531ffQ8ht5yVnHH5eVdaHIbktqVgTJYMSsbhDVlw1UABLYcrsmCqVYWspbom71PaMcZrJsU7cs1lI0CwdkEOaxNNFw7OmoEan802eJf2NPR0vVwt1zSixTGHSOcpbwbnt3RzpE8Pa-o8fUYfxinREOwxY6KvztD8gjQfRyxW1Jsc3RQxO0vHucV1-LfvA3nbmyHhx8u9Ib--3v9cfS8ef3x7WC0fCwsSctEI21QKFevBGolNLXBT80Z2vG4rDrXatFJ1wLAFK1TVI2-rDnsupOIAwsANuTv3jjH8njBlvXfppM54DFPSUrXAKsVmsD6DNoaUIvZ6jG5v4lFzpk_K9U5flOuTcs1Az8rn3KfLwLTZY_cvdXE8A1_OAM5vvjqMOlmH3mLnZhlZd8H9Z-IPdN-VgQ</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Lelièvre, V.</creator><creator>Hu, Z.</creator><creator>Ioffe, Y.</creator><creator>Byun, J.-Y.</creator><creator>Flores, A.</creator><creator>Seksenyan, A.</creator><creator>Waschek, J.A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20061101</creationdate><title>Paradoxical antagonism of PACAP receptor signaling by VIP in Xenopus oocytes via the type-C natriuretic peptide receptor</title><author>Lelièvre, V. ; Hu, Z. ; Ioffe, Y. ; Byun, J.-Y. ; Flores, A. ; Seksenyan, A. ; Waschek, J.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-72c748e80f3ca6e752eb5176d15941358b968d30e93c284fe194def12681332a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adenylyl Cyclases - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Binding, Competitive</topic><topic>cAMP</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - genetics</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Natriuretic peptide</topic><topic>Neuropeptide</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - metabolism</topic><topic>Rats</topic><topic>Receptor</topic><topic>Receptors, Atrial Natriuretic Factor - genetics</topic><topic>Receptors, Atrial Natriuretic Factor - metabolism</topic><topic>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide - antagonists & inhibitors</topic><topic>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Messenger - pharmacology</topic><topic>Sequence Alignment</topic><topic>Signal Transduction</topic><topic>Signaling</topic><topic>Vasoactive Intestinal Peptide - metabolism</topic><topic>Vasoactive Intestinal Peptide - pharmacology</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lelièvre, V.</creatorcontrib><creatorcontrib>Hu, Z.</creatorcontrib><creatorcontrib>Ioffe, Y.</creatorcontrib><creatorcontrib>Byun, J.-Y.</creatorcontrib><creatorcontrib>Flores, A.</creatorcontrib><creatorcontrib>Seksenyan, A.</creatorcontrib><creatorcontrib>Waschek, J.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lelièvre, V.</au><au>Hu, Z.</au><au>Ioffe, Y.</au><au>Byun, J.-Y.</au><au>Flores, A.</au><au>Seksenyan, A.</au><au>Waschek, J.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paradoxical antagonism of PACAP receptor signaling by VIP in Xenopus oocytes via the type-C natriuretic peptide receptor</atitle><jtitle>Cellular signalling</jtitle><addtitle>Cell Signal</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>18</volume><issue>11</issue><spage>2013</spage><epage>2021</epage><pages>2013-2021</pages><issn>0898-6568</issn><eissn>1873-3913</eissn><abstract>Atrial natriuretic peptide (ANP) and the closely-related peptides BNP and CNP are highly conserved cardiovascular hormones. They bind to single transmembrane-spanning receptors, triggering receptor-intrinsic guanylyl cyclase activity. The “truncated” type-C natriuretic peptide receptor (NPR-C) has long been called a clearance receptor because it lacks the intracellular guanylyl cyclase domain, though data suggest it might negatively couple to adenylyl cyclase via G
i. Here we report the molecular cloning and characterization of the
Xenopus laevis type-C natriuretic peptide receptor (
XNPR-C). Analysis confirms the presence of a short intracellular C-terminus, as well as a high similarity to fish and mammalian NPR-C. Injection of
XNPR-C mRNA into
Xenopus oocytes resulted in expression of high affinity [
125I]ANP binding sites that were competitively and completely displaced by natriuretic analogs and the unrelated neuropeptide vasoactive intestinal peptide (VIP). Measurement of cAMP levels in mRNA-injected oocytes revealed that
XNPR-C is negatively coupled to adenylyl cyclase in a pertussis toxin-sensitive manner. When
XNPR-C was co-expressed with PAC
1 receptors for pituitary adenylyl cyclase-activating polypeptide (PACAP), VIP and natriuretic peptides counteracted the cAMP induction by PACAP. These results suggest that VIP and natriuretic peptides can potentially modulate the action of PACAP in cells where these receptors are co-expressed.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>16723209</pmid><doi>10.1016/j.cellsig.2006.03.013</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0898-6568 |
| ispartof | Cellular signalling, 2006-11, Vol.18 (11), p.2013-2021 |
| issn | 0898-6568 1873-3913 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adenylyl Cyclases - metabolism Amino Acid Sequence Animals Binding, Competitive cAMP Cloning, Molecular DNA, Complementary - genetics Humans Molecular Sequence Data Natriuretic peptide Neuropeptide Oocytes - drug effects Oocytes - metabolism Rats Receptor Receptors, Atrial Natriuretic Factor - genetics Receptors, Atrial Natriuretic Factor - metabolism Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide - antagonists & inhibitors Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide - metabolism RNA, Messenger - metabolism RNA, Messenger - pharmacology Sequence Alignment Signal Transduction Signaling Vasoactive Intestinal Peptide - metabolism Vasoactive Intestinal Peptide - pharmacology Xenopus |
| title | Paradoxical antagonism of PACAP receptor signaling by VIP in Xenopus oocytes via the type-C natriuretic peptide receptor |
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