Continuous Glucose Detection Using Boronic Acid-Substituted Viologens in Fluorescent Hydrogels:  Linker Effects and Extension to Fiber Optics

A fluorescent anionic dye and a viologen appended with boronic acids, which serve as glucose receptors, have been synthesized and immobilized into a poly(2-hydroxyethyl methacrylate) hydrogel for use as a continuous glucose monitor. The fluorescence of the dye is modulated by the quenching efficienc...

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Veröffentlicht in:Langmuir 2006-10, Vol.22 (21), p.9067-9074
Hauptverfasser: Gamsey, Soya, Suri, Jeff T, Wessling, Ritchie A, Singaram, Bakthan
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creator Gamsey, Soya
Suri, Jeff T
Wessling, Ritchie A
Singaram, Bakthan
description A fluorescent anionic dye and a viologen appended with boronic acids, which serve as glucose receptors, have been synthesized and immobilized into a poly(2-hydroxyethyl methacrylate) hydrogel for use as a continuous glucose monitor. The fluorescence of the dye is modulated by the quenching efficiency of the viologen-based receptor, which in turn is dependent on the glucose concentration. Two monomeric versions of the quencher/receptor unit were prepared and their performance within the hydrogel evaluated. By tethering the quencher/receptor to the hydrogel matrix using a single-point attachment, slightly improved glucose sensing was observed. The hydrogels were tested for their ability to continuously and reversibly detect glucose over the course of several hours. The tests were carried out using a cuvette-based system, as well as a fiber-optic-based configuration. Under physiological conditions (0.1 M phosphate buffer, pH 7.4, 37 °C), the fluorescent hydrogels display an excellent dynamic response to glucose concentrations within the biologically significant range (2.5−20 mM).
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subjects Biosensing Techniques
Boronic Acids - chemistry
Chemistry
Colloidal gels. Colloidal sols
Colloidal state and disperse state
Cross-Linking Reagents - chemistry
Exact sciences and technology
Fiber Optic Technology
Fluorescent Dyes - chemistry
General and physical chemistry
Glucose - analysis
Glucose - chemistry
Hydrogels - chemistry
Optical Fibers
Polymers - chemistry
Viologens - chemistry
title Continuous Glucose Detection Using Boronic Acid-Substituted Viologens in Fluorescent Hydrogels:  Linker Effects and Extension to Fiber Optics
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