Antibacterial properties of EMLA® and lidocaine in wound tissue biopsies for culturing
ABSTRACT If a tissue biopsy from a chronic wound is sampled for culture, the antibacterial properties of local anesthetics may pose a problem in producing false‐negative results. The purpose of this study was to investigate the effects of EMLA® (AstraZeneca) and lidocaine on common wound pathogenic...
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| Veröffentlicht in: | Wound repair and regeneration 2006-09, Vol.14 (5), p.581-585 |
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| container_title | Wound repair and regeneration |
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| creator | Berg, Jais O. Mössner, Belinda K. Skov, Marianne N. Lauridsen, Jorgen Gottrup, Finn Kolmos, Hans J. |
| description | ABSTRACT
If a tissue biopsy from a chronic wound is sampled for culture, the antibacterial properties of local anesthetics may pose a problem in producing false‐negative results. The purpose of this study was to investigate the effects of EMLA® (AstraZeneca) and lidocaine on common wound pathogenic bacteria. An in vitro study of a total of 25 clinical isolates and ATCC reference strains of Staphylococcus aureus (including methicillin‐resistant S. aureus), Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes was conducted. The isolates were exposed to the local anesthetic drugs (and some of their contents separately) at 35°C over a 24‐hour period and time–kill curves were recorded. No culture media were used and saline was used for controls. EMLA® was found to have a rapid acting and powerful antibacterial effect and should not be used before culturing tissue samples. Lidocaine 1% was found not to inhibit the bacterial strains when exposure time was held below 2 hours. We conclude that culturing tissue from a wound biopsy is safe within 2 hours when a pure, preservative‐free lidocaine 1% solution is used. |
| doi_str_mv | 10.1111/j.1743-6109.2006.00157.x |
| format | Article |
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If a tissue biopsy from a chronic wound is sampled for culture, the antibacterial properties of local anesthetics may pose a problem in producing false‐negative results. The purpose of this study was to investigate the effects of EMLA® (AstraZeneca) and lidocaine on common wound pathogenic bacteria. An in vitro study of a total of 25 clinical isolates and ATCC reference strains of Staphylococcus aureus (including methicillin‐resistant S. aureus), Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes was conducted. The isolates were exposed to the local anesthetic drugs (and some of their contents separately) at 35°C over a 24‐hour period and time–kill curves were recorded. No culture media were used and saline was used for controls. EMLA® was found to have a rapid acting and powerful antibacterial effect and should not be used before culturing tissue samples. Lidocaine 1% was found not to inhibit the bacterial strains when exposure time was held below 2 hours. We conclude that culturing tissue from a wound biopsy is safe within 2 hours when a pure, preservative‐free lidocaine 1% solution is used.</description><identifier>ISSN: 1067-1927</identifier><identifier>EISSN: 1524-475X</identifier><identifier>DOI: 10.1111/j.1743-6109.2006.00157.x</identifier><identifier>PMID: 17014670</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Anesthetics, Local - pharmacology ; Anti-Bacterial Agents - pharmacology ; Biopsy ; Cells, Cultured ; Chronic Disease ; Colony Count, Microbial ; Escherichia coli - drug effects ; Escherichia coli - isolation & purification ; False Negative Reactions ; Humans ; Lidocaine - pharmacology ; Microbial Sensitivity Tests ; Prilocaine - pharmacology ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - isolation & purification ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - isolation & purification ; Streptococcus pyogenes - drug effects ; Streptococcus pyogenes - isolation & purification ; Wound Infection - microbiology</subject><ispartof>Wound repair and regeneration, 2006-09, Vol.14 (5), p.581-585</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4297-36434c9b13f4db4996a7a36a6f6b6456b3523fcf7e8a2d7ec721df643c942fae3</citedby><cites>FETCH-LOGICAL-c4297-36434c9b13f4db4996a7a36a6f6b6456b3523fcf7e8a2d7ec721df643c942fae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1743-6109.2006.00157.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1743-6109.2006.00157.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17014670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Berg, Jais O.</creatorcontrib><creatorcontrib>Mössner, Belinda K.</creatorcontrib><creatorcontrib>Skov, Marianne N.</creatorcontrib><creatorcontrib>Lauridsen, Jorgen</creatorcontrib><creatorcontrib>Gottrup, Finn</creatorcontrib><creatorcontrib>Kolmos, Hans J.</creatorcontrib><title>Antibacterial properties of EMLA® and lidocaine in wound tissue biopsies for culturing</title><title>Wound repair and regeneration</title><addtitle>Wound Repair Regen</addtitle><description>ABSTRACT
If a tissue biopsy from a chronic wound is sampled for culture, the antibacterial properties of local anesthetics may pose a problem in producing false‐negative results. The purpose of this study was to investigate the effects of EMLA® (AstraZeneca) and lidocaine on common wound pathogenic bacteria. An in vitro study of a total of 25 clinical isolates and ATCC reference strains of Staphylococcus aureus (including methicillin‐resistant S. aureus), Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes was conducted. The isolates were exposed to the local anesthetic drugs (and some of their contents separately) at 35°C over a 24‐hour period and time–kill curves were recorded. No culture media were used and saline was used for controls. EMLA® was found to have a rapid acting and powerful antibacterial effect and should not be used before culturing tissue samples. Lidocaine 1% was found not to inhibit the bacterial strains when exposure time was held below 2 hours. We conclude that culturing tissue from a wound biopsy is safe within 2 hours when a pure, preservative‐free lidocaine 1% solution is used.</description><subject>Anesthetics, Local - pharmacology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biopsy</subject><subject>Cells, Cultured</subject><subject>Chronic Disease</subject><subject>Colony Count, Microbial</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - isolation & purification</subject><subject>False Negative Reactions</subject><subject>Humans</subject><subject>Lidocaine - pharmacology</subject><subject>Microbial Sensitivity Tests</subject><subject>Prilocaine - pharmacology</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Streptococcus pyogenes - drug effects</subject><subject>Streptococcus pyogenes - isolation & purification</subject><subject>Wound Infection - microbiology</subject><issn>1067-1927</issn><issn>1524-475X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtO6zAQhi0E4v4KyKuzS_Atdi2xqRCHHlRAQqAiNpbj2MglTYqdiPJSPARPhkMrzpbZzMj6vxnrAwBilONUp_McC0YzjpHMCUI8RwgXIl9tgX1cEJYxUTxupxlxkWFJxB44iHGOECoKOdoFe1ggzLhA-2A2bjpfatPZ4HUNl6Fd2tB5G2Hr4MX1dPz5AXVTwdpXrdG-sdA38K3t01PnY-wtLH27jAPg2gBNX3d98M3zEdhxuo72eNMPwcPfi_vzSTa9vfx3Pp5mhhEpMsoZZUaWmDpWlUxKroWmXHPHS84KXtKCUGecsCNNKmGNILhyCTKSEactPQR_1nvTz197Gzu18NHYutaNbfuo-EgSiQVNwdE6aEIbY7BOLYNf6PCuMFKDVDVXg1Q1SFWDVPUtVa0SerK50ZcLW_0HNxZT4GwdePO1ff_1YjW7u0tDwrM17mNnVz-4Di-KCyoKNbu5VFeTpxnF8klN6BdJeJXz</recordid><startdate>200609</startdate><enddate>200609</enddate><creator>Berg, Jais O.</creator><creator>Mössner, Belinda K.</creator><creator>Skov, Marianne N.</creator><creator>Lauridsen, Jorgen</creator><creator>Gottrup, Finn</creator><creator>Kolmos, Hans J.</creator><general>Blackwell Publishing Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200609</creationdate><title>Antibacterial properties of EMLA® and lidocaine in wound tissue biopsies for culturing</title><author>Berg, Jais O. ; Mössner, Belinda K. ; Skov, Marianne N. ; Lauridsen, Jorgen ; Gottrup, Finn ; Kolmos, Hans J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4297-36434c9b13f4db4996a7a36a6f6b6456b3523fcf7e8a2d7ec721df643c942fae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Anesthetics, Local - pharmacology</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biopsy</topic><topic>Cells, Cultured</topic><topic>Chronic Disease</topic><topic>Colony Count, Microbial</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - isolation & purification</topic><topic>False Negative Reactions</topic><topic>Humans</topic><topic>Lidocaine - pharmacology</topic><topic>Microbial Sensitivity Tests</topic><topic>Prilocaine - pharmacology</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - isolation & purification</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Streptococcus pyogenes - drug effects</topic><topic>Streptococcus pyogenes - isolation & purification</topic><topic>Wound Infection - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Berg, Jais O.</creatorcontrib><creatorcontrib>Mössner, Belinda K.</creatorcontrib><creatorcontrib>Skov, Marianne N.</creatorcontrib><creatorcontrib>Lauridsen, Jorgen</creatorcontrib><creatorcontrib>Gottrup, Finn</creatorcontrib><creatorcontrib>Kolmos, Hans J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Wound repair and regeneration</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Berg, Jais O.</au><au>Mössner, Belinda K.</au><au>Skov, Marianne N.</au><au>Lauridsen, Jorgen</au><au>Gottrup, Finn</au><au>Kolmos, Hans J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial properties of EMLA® and lidocaine in wound tissue biopsies for culturing</atitle><jtitle>Wound repair and regeneration</jtitle><addtitle>Wound Repair Regen</addtitle><date>2006-09</date><risdate>2006</risdate><volume>14</volume><issue>5</issue><spage>581</spage><epage>585</epage><pages>581-585</pages><issn>1067-1927</issn><eissn>1524-475X</eissn><abstract>ABSTRACT
If a tissue biopsy from a chronic wound is sampled for culture, the antibacterial properties of local anesthetics may pose a problem in producing false‐negative results. The purpose of this study was to investigate the effects of EMLA® (AstraZeneca) and lidocaine on common wound pathogenic bacteria. An in vitro study of a total of 25 clinical isolates and ATCC reference strains of Staphylococcus aureus (including methicillin‐resistant S. aureus), Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes was conducted. The isolates were exposed to the local anesthetic drugs (and some of their contents separately) at 35°C over a 24‐hour period and time–kill curves were recorded. No culture media were used and saline was used for controls. EMLA® was found to have a rapid acting and powerful antibacterial effect and should not be used before culturing tissue samples. Lidocaine 1% was found not to inhibit the bacterial strains when exposure time was held below 2 hours. We conclude that culturing tissue from a wound biopsy is safe within 2 hours when a pure, preservative‐free lidocaine 1% solution is used.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>17014670</pmid><doi>10.1111/j.1743-6109.2006.00157.x</doi><tpages>5</tpages></addata></record> |
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| subjects | Anesthetics, Local - pharmacology Anti-Bacterial Agents - pharmacology Biopsy Cells, Cultured Chronic Disease Colony Count, Microbial Escherichia coli - drug effects Escherichia coli - isolation & purification False Negative Reactions Humans Lidocaine - pharmacology Microbial Sensitivity Tests Prilocaine - pharmacology Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - isolation & purification Staphylococcus aureus - drug effects Staphylococcus aureus - isolation & purification Streptococcus pyogenes - drug effects Streptococcus pyogenes - isolation & purification Wound Infection - microbiology |
| title | Antibacterial properties of EMLA® and lidocaine in wound tissue biopsies for culturing |
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