Humoral and cellular immunity induced by tumor cell vaccine based on the chicken xenogeneic homologous matrix metalloproteinase-2
Matrix metalloproteinase-2 (MMP-2) has been used as a target for cancer immunotherapy. The activation of immunization by breaking immune tolerance to self-MMP-2 may be one of the promising approaches for the treatment of MMP-2-positive tumors. In this study, we constructed the xenogeneic tumor cell...
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| Veröffentlicht in: | Cancer gene therapy 2007-02, Vol.14 (2), p.158-164 |
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| creator | Yi, T Wei, Y-Q Tian, L Zhao, X Li, J Deng, H-X Wen, Y-J Zou, C-H Tan, G-H Kan, B Su, J-M Jiang, Y Mao, Y-Q Chen, P Wang, Y-S |
| description | Matrix metalloproteinase-2 (MMP-2) has been used as a target for cancer immunotherapy. The activation of immunization by breaking immune tolerance to self-MMP-2 may be one of the promising approaches for the treatment of MMP-2-positive tumors. In this study, we constructed the xenogeneic tumor cell vaccine c-MMP-2 by transfecting CT26 and LLC cells with chicken MMP-2 cDNA constructs. MMP-2-specific autoantibodies in sera and tumor cells were found in mice immunized with c-MMP-2. Protection against tumor growth was evaluated in respect of the relative contributions of autoantibodies, CD4+, and CD8+ T cells. Treatment with this vaccine (c-MMP-2) also prolonged the survival time of mice bearing cancer. The specific cytotoxic T-cell responses suggested that the treatment increased CD8+ T-cell activity. The antitumor activity of c-MMP-2 was abrogated by
in vivo
depletion of CD4+ and CD8+ T-lymphocytes and improved by adoptive transfer of CD4+ and CD8+ T-lymphocytes from the mice treated with c-MMP-2. An alternative DNA vaccination strategy for cancer therapy was identified in this study by eliciting humoral and cellular immunoresponse with a crossreacting transfectant. |
| doi_str_mv | 10.1038/sj.cgt.7700994 |
| format | Article |
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in vivo
depletion of CD4+ and CD8+ T-lymphocytes and improved by adoptive transfer of CD4+ and CD8+ T-lymphocytes from the mice treated with c-MMP-2. An alternative DNA vaccination strategy for cancer therapy was identified in this study by eliciting humoral and cellular immunoresponse with a crossreacting transfectant.</description><identifier>ISSN: 0929-1903</identifier><identifier>EISSN: 1476-5500</identifier><identifier>DOI: 10.1038/sj.cgt.7700994</identifier><identifier>PMID: 17124509</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Adoptive transfer ; Animals ; Antibody Formation ; Antitumor activity ; Autoantibodies ; Autoantibodies - immunology ; Base Sequence ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Cancer ; Cancer immunotherapy ; Cancer Vaccines - immunology ; Care and treatment ; CD4 antigen ; CD8 antigen ; Cell Line, Tumor ; Cell-mediated immunity ; Cellular immunity ; Chickens ; Colonic Neoplasms - immunology ; Cytotoxicity ; DNA Primers ; DNA vaccines ; DNA, Complementary ; Gelatinase A ; Gene Expression ; Gene Therapy ; Genetic aspects ; Health aspects ; Humoral immunity ; Immunity, Cellular ; Immunohistochemistry ; Immunological tolerance ; Immunotherapy ; Lymphocytes T ; Matrix metalloproteinase ; Matrix Metalloproteinase 2 - genetics ; Metalloproteinase ; Methods ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Neovascularization, Pathologic ; original-article ; Reverse Transcriptase Polymerase Chain Reaction ; T-Lymphocyte Subsets ; Tumor cells ; Vaccination ; Vaccines ; Vaccines, DNA - immunology</subject><ispartof>Cancer gene therapy, 2007-02, Vol.14 (2), p.158-164</ispartof><rights>Springer Nature America, Inc. 2007</rights><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Feb 2007</rights><rights>Nature Publishing Group 2007.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c579t-8c9d4cade20fb264f738a7290297f8da87029447342321b55d7c23b901c1a53b3</citedby><cites>FETCH-LOGICAL-c579t-8c9d4cade20fb264f738a7290297f8da87029447342321b55d7c23b901c1a53b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/sj.cgt.7700994$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/sj.cgt.7700994$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17124509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yi, T</creatorcontrib><creatorcontrib>Wei, Y-Q</creatorcontrib><creatorcontrib>Tian, L</creatorcontrib><creatorcontrib>Zhao, X</creatorcontrib><creatorcontrib>Li, J</creatorcontrib><creatorcontrib>Deng, H-X</creatorcontrib><creatorcontrib>Wen, Y-J</creatorcontrib><creatorcontrib>Zou, C-H</creatorcontrib><creatorcontrib>Tan, G-H</creatorcontrib><creatorcontrib>Kan, B</creatorcontrib><creatorcontrib>Su, J-M</creatorcontrib><creatorcontrib>Jiang, Y</creatorcontrib><creatorcontrib>Mao, Y-Q</creatorcontrib><creatorcontrib>Chen, P</creatorcontrib><creatorcontrib>Wang, Y-S</creatorcontrib><title>Humoral and cellular immunity induced by tumor cell vaccine based on the chicken xenogeneic homologous matrix metalloproteinase-2</title><title>Cancer gene therapy</title><addtitle>Cancer Gene Ther</addtitle><addtitle>Cancer Gene Ther</addtitle><description>Matrix metalloproteinase-2 (MMP-2) has been used as a target for cancer immunotherapy. The activation of immunization by breaking immune tolerance to self-MMP-2 may be one of the promising approaches for the treatment of MMP-2-positive tumors. In this study, we constructed the xenogeneic tumor cell vaccine c-MMP-2 by transfecting CT26 and LLC cells with chicken MMP-2 cDNA constructs. MMP-2-specific autoantibodies in sera and tumor cells were found in mice immunized with c-MMP-2. Protection against tumor growth was evaluated in respect of the relative contributions of autoantibodies, CD4+, and CD8+ T cells. Treatment with this vaccine (c-MMP-2) also prolonged the survival time of mice bearing cancer. The specific cytotoxic T-cell responses suggested that the treatment increased CD8+ T-cell activity. The antitumor activity of c-MMP-2 was abrogated by
in vivo
depletion of CD4+ and CD8+ T-lymphocytes and improved by adoptive transfer of CD4+ and CD8+ T-lymphocytes from the mice treated with c-MMP-2. An alternative DNA vaccination strategy for cancer therapy was identified in this study by eliciting humoral and cellular immunoresponse with a crossreacting transfectant.</description><subject>Adoptive transfer</subject><subject>Animals</subject><subject>Antibody Formation</subject><subject>Antitumor activity</subject><subject>Autoantibodies</subject><subject>Autoantibodies - immunology</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Cancer</subject><subject>Cancer immunotherapy</subject><subject>Cancer Vaccines - immunology</subject><subject>Care and treatment</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Cell Line, Tumor</subject><subject>Cell-mediated immunity</subject><subject>Cellular immunity</subject><subject>Chickens</subject><subject>Colonic Neoplasms - immunology</subject><subject>Cytotoxicity</subject><subject>DNA Primers</subject><subject>DNA vaccines</subject><subject>DNA, Complementary</subject><subject>Gelatinase A</subject><subject>Gene Expression</subject><subject>Gene Therapy</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humoral immunity</subject><subject>Immunity, Cellular</subject><subject>Immunohistochemistry</subject><subject>Immunological tolerance</subject><subject>Immunotherapy</subject><subject>Lymphocytes T</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 2 - genetics</subject><subject>Metalloproteinase</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Neovascularization, Pathologic</subject><subject>original-article</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>T-Lymphocyte Subsets</subject><subject>Tumor cells</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Vaccines, DNA - immunology</subject><issn>0929-1903</issn><issn>1476-5500</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkktv1DAUhSMEokNhyw5kgdRdpn4ljpdVBRSpEhtYW47jZDw4dvGj6iz55zjMwABqxcqWz3fOvde6VfUSwTWCpDuP27Wa0poxCDmnj6oVoqytmwbCx9UKcsxrxCE5qZ7FuIWwiIw8rU4QQ5g2kK-q71d59kFaIN0AlLY2WxmAmefsTNoB44as9AD6HUgL-BMBt1Ip4zToZSyadyBtNFAbo75qB-6085N22iiw8bO3fvI5glmmYO7ArJO01t8En7RxxV7j59WTUdqoXxzO0-rL-3efL6_q608fPl5eXNeqYTzVneIDVXLQGI49bunISCcZ5hBzNnaD7Fi5UcoIxQSjvmkGpjDpOUQKyYb05LQ62-eW4t-yjknMJi7jSKdLh6LtOCYd4_8FEWcEsRYV8O0_4Nbn4MoQojRY_phSvMS9eZBCjHLCWHuMmqTVwrjRpyDVUldcoA7jljcNLdT6HkouvzIb5Z0eTXn_y3D2h2GjpU2b6G1Oxrt4b7IKPsagR3ETzCzDTiAolkUTcSvKoonDohXD68NUuZ_1cMQPm1WA8z0Qi-QmHY5jPxj5au9wMuWgf0f-0n8AMgbmqA</recordid><startdate>200702</startdate><enddate>200702</enddate><creator>Yi, T</creator><creator>Wei, Y-Q</creator><creator>Tian, L</creator><creator>Zhao, X</creator><creator>Li, J</creator><creator>Deng, H-X</creator><creator>Wen, Y-J</creator><creator>Zou, C-H</creator><creator>Tan, G-H</creator><creator>Kan, B</creator><creator>Su, J-M</creator><creator>Jiang, Y</creator><creator>Mao, Y-Q</creator><creator>Chen, P</creator><creator>Wang, Y-S</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QO</scope><scope>7T5</scope><scope>7X8</scope></search><sort><creationdate>200702</creationdate><title>Humoral and cellular immunity induced by tumor cell vaccine based on the chicken xenogeneic homologous matrix metalloproteinase-2</title><author>Yi, T ; Wei, Y-Q ; Tian, L ; Zhao, X ; Li, J ; Deng, H-X ; Wen, Y-J ; Zou, C-H ; Tan, G-H ; Kan, B ; Su, J-M ; Jiang, Y ; Mao, Y-Q ; Chen, P ; Wang, Y-S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c579t-8c9d4cade20fb264f738a7290297f8da87029447342321b55d7c23b901c1a53b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adoptive transfer</topic><topic>Animals</topic><topic>Antibody Formation</topic><topic>Antitumor activity</topic><topic>Autoantibodies</topic><topic>Autoantibodies - 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Academic</collection><jtitle>Cancer gene therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yi, T</au><au>Wei, Y-Q</au><au>Tian, L</au><au>Zhao, X</au><au>Li, J</au><au>Deng, H-X</au><au>Wen, Y-J</au><au>Zou, C-H</au><au>Tan, G-H</au><au>Kan, B</au><au>Su, J-M</au><au>Jiang, Y</au><au>Mao, Y-Q</au><au>Chen, P</au><au>Wang, Y-S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Humoral and cellular immunity induced by tumor cell vaccine based on the chicken xenogeneic homologous matrix metalloproteinase-2</atitle><jtitle>Cancer gene therapy</jtitle><stitle>Cancer Gene Ther</stitle><addtitle>Cancer Gene Ther</addtitle><date>2007-02</date><risdate>2007</risdate><volume>14</volume><issue>2</issue><spage>158</spage><epage>164</epage><pages>158-164</pages><issn>0929-1903</issn><eissn>1476-5500</eissn><abstract>Matrix metalloproteinase-2 (MMP-2) has been used as a target for cancer immunotherapy. The activation of immunization by breaking immune tolerance to self-MMP-2 may be one of the promising approaches for the treatment of MMP-2-positive tumors. In this study, we constructed the xenogeneic tumor cell vaccine c-MMP-2 by transfecting CT26 and LLC cells with chicken MMP-2 cDNA constructs. MMP-2-specific autoantibodies in sera and tumor cells were found in mice immunized with c-MMP-2. Protection against tumor growth was evaluated in respect of the relative contributions of autoantibodies, CD4+, and CD8+ T cells. Treatment with this vaccine (c-MMP-2) also prolonged the survival time of mice bearing cancer. The specific cytotoxic T-cell responses suggested that the treatment increased CD8+ T-cell activity. The antitumor activity of c-MMP-2 was abrogated by
in vivo
depletion of CD4+ and CD8+ T-lymphocytes and improved by adoptive transfer of CD4+ and CD8+ T-lymphocytes from the mice treated with c-MMP-2. An alternative DNA vaccination strategy for cancer therapy was identified in this study by eliciting humoral and cellular immunoresponse with a crossreacting transfectant.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>17124509</pmid><doi>10.1038/sj.cgt.7700994</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Springer Online Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adoptive transfer Animals Antibody Formation Antitumor activity Autoantibodies Autoantibodies - immunology Base Sequence Biomedical and Life Sciences Biomedicine Blotting, Western Cancer Cancer immunotherapy Cancer Vaccines - immunology Care and treatment CD4 antigen CD8 antigen Cell Line, Tumor Cell-mediated immunity Cellular immunity Chickens Colonic Neoplasms - immunology Cytotoxicity DNA Primers DNA vaccines DNA, Complementary Gelatinase A Gene Expression Gene Therapy Genetic aspects Health aspects Humoral immunity Immunity, Cellular Immunohistochemistry Immunological tolerance Immunotherapy Lymphocytes T Matrix metalloproteinase Matrix Metalloproteinase 2 - genetics Metalloproteinase Methods Mice Mice, Inbred BALB C Mice, Inbred C57BL Neovascularization, Pathologic original-article Reverse Transcriptase Polymerase Chain Reaction T-Lymphocyte Subsets Tumor cells Vaccination Vaccines Vaccines, DNA - immunology |
| title | Humoral and cellular immunity induced by tumor cell vaccine based on the chicken xenogeneic homologous matrix metalloproteinase-2 |
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