Precolumn diastereomerization and micellar electrokinetic chromatography on a plastic microchip: Rapid chiral analysis of amino acids
Precolumn derivatization and chiral separation of DL‐amino acids based on diastereomerization have been performed on an integrated poly(dimethylsiloxane) microchip. Diastereomeric derivatives were formed in a microfabricated precolumn reactor by the reaction of amino acid enantiomers with o‐phthaldi...
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| Veröffentlicht in: | Electrophoresis 2005-12, Vol.26 (24), p.4767-4773 |
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| creator | Ro, Kyung Won Hahn, Jong Hoon |
| description | Precolumn derivatization and chiral separation of DL‐amino acids based on diastereomerization have been performed on an integrated poly(dimethylsiloxane) microchip. Diastereomeric derivatives were formed in a microfabricated precolumn reactor by the reaction of amino acid enantiomers with o‐phthaldialdehyde/2,3,4,6‐tetra‐O‐acetyl‐1‐thio‐β‐D‐glucopyranose (OPA/TATG), and separated by MEKC in an achiral environment without chiral selectors in the running buffer. Optimized precolumn reactions and chiral separations of amino acids were achieved within 2.5 min. Resolutions of diastereomers of OPA/TATG‐amino acids were in the range of 2.5–6.1 at optimized separation conditions. Simultaneous separation of a mixture of five chiral amino acids was successfully performed in a single run in less than 100 s. |
| doi_str_mv | 10.1002/elps.200500370 |
| format | Article |
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Diastereomeric derivatives were formed in a microfabricated precolumn reactor by the reaction of amino acid enantiomers with o‐phthaldialdehyde/2,3,4,6‐tetra‐O‐acetyl‐1‐thio‐β‐D‐glucopyranose (OPA/TATG), and separated by MEKC in an achiral environment without chiral selectors in the running buffer. Optimized precolumn reactions and chiral separations of amino acids were achieved within 2.5 min. Resolutions of diastereomers of OPA/TATG‐amino acids were in the range of 2.5–6.1 at optimized separation conditions. Simultaneous separation of a mixture of five chiral amino acids was successfully performed in a single run in less than 100 s.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.200500370</identifier><identifier>PMID: 16278921</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Amino acids ; Amino Acids - analysis ; Amino Acids - isolation & purification ; Chiral analysis ; Chromatography, Micellar Electrokinetic Capillary - methods ; Diastereomerization ; Dimethylpolysiloxanes ; Electrophoresis, Microchip - methods ; Glucose - analogs & derivatives ; Glucose - chemistry ; Miniaturization ; o-Phthalaldehyde - chemistry ; Plastic microchip ; Precolumn reaction ; Silicones ; Stereoisomerism ; Sulfhydryl Compounds - chemistry</subject><ispartof>Electrophoresis, 2005-12, Vol.26 (24), p.4767-4773</ispartof><rights>Copyright © 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3810-6d7eb09d35c1463f646285dac026d9942e9a3c0a2e7ba042048b76822ae517dc3</citedby><cites>FETCH-LOGICAL-c3810-6d7eb09d35c1463f646285dac026d9942e9a3c0a2e7ba042048b76822ae517dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.200500370$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16278921$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ro, Kyung Won</creatorcontrib><creatorcontrib>Hahn, Jong Hoon</creatorcontrib><title>Precolumn diastereomerization and micellar electrokinetic chromatography on a plastic microchip: Rapid chiral analysis of amino acids</title><title>Electrophoresis</title><addtitle>ELECTROPHORESIS</addtitle><description>Precolumn derivatization and chiral separation of DL‐amino acids based on diastereomerization have been performed on an integrated poly(dimethylsiloxane) microchip. Diastereomeric derivatives were formed in a microfabricated precolumn reactor by the reaction of amino acid enantiomers with o‐phthaldialdehyde/2,3,4,6‐tetra‐O‐acetyl‐1‐thio‐β‐D‐glucopyranose (OPA/TATG), and separated by MEKC in an achiral environment without chiral selectors in the running buffer. Optimized precolumn reactions and chiral separations of amino acids were achieved within 2.5 min. Resolutions of diastereomers of OPA/TATG‐amino acids were in the range of 2.5–6.1 at optimized separation conditions. Simultaneous separation of a mixture of five chiral amino acids was successfully performed in a single run in less than 100 s.</description><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Amino Acids - isolation & purification</subject><subject>Chiral analysis</subject><subject>Chromatography, Micellar Electrokinetic Capillary - methods</subject><subject>Diastereomerization</subject><subject>Dimethylpolysiloxanes</subject><subject>Electrophoresis, Microchip - methods</subject><subject>Glucose - analogs & derivatives</subject><subject>Glucose - chemistry</subject><subject>Miniaturization</subject><subject>o-Phthalaldehyde - chemistry</subject><subject>Plastic microchip</subject><subject>Precolumn reaction</subject><subject>Silicones</subject><subject>Stereoisomerism</subject><subject>Sulfhydryl Compounds - chemistry</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT2PEzEQhi0E4o6DlhK5otswtne9Xjq4LxABThB0dNbEdog573qxN4LQ879xSHTQUU0xz_tI8w4hjxnMGAB_5sKYZxygARAt3CHHrOG84lKJu-QYWCsqUKI5Ig9y_goAdVfX98kRk7xVHWfH5NdVciaGTT9Q6zFPLrnYu-R_4uTjQHGwtPfGhYCJuuDMlOKNH9zkDTXrFHuc4peE43pLdzQdQ3GUXcmkaNZ-fE4_4OhtgX3CUHwYttlnGlcUez9Eisbb_JDcW2HI7tFhnpBPF-eL01fV_P3l69MX88oIxaCStnVL6KxoDKulWMlactVYNMCl7bqauw6FAeSuXSLUHGq1bKXiHF3DWmvECXm6944pftu4POne5z_XDS5uspaqY0o1XQFne7CckXNyKz0m32PaagZ6V7zeFa9viy-BJwfzZtk7-xc_NF2Abg9898Ft_6PT5_Orj__Kq33Wlwf9uM1iutGyFW2jr99d6jdnLz-_XVxf6IX4DYfhohs</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Ro, Kyung Won</creator><creator>Hahn, Jong Hoon</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Precolumn diastereomerization and micellar electrokinetic chromatography on a plastic microchip: Rapid chiral analysis of amino acids</title><author>Ro, Kyung Won ; Hahn, Jong Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3810-6d7eb09d35c1463f646285dac026d9942e9a3c0a2e7ba042048b76822ae517dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino acids</topic><topic>Amino Acids - analysis</topic><topic>Amino Acids - isolation & purification</topic><topic>Chiral analysis</topic><topic>Chromatography, Micellar Electrokinetic Capillary - methods</topic><topic>Diastereomerization</topic><topic>Dimethylpolysiloxanes</topic><topic>Electrophoresis, Microchip - methods</topic><topic>Glucose - analogs & derivatives</topic><topic>Glucose - chemistry</topic><topic>Miniaturization</topic><topic>o-Phthalaldehyde - chemistry</topic><topic>Plastic microchip</topic><topic>Precolumn reaction</topic><topic>Silicones</topic><topic>Stereoisomerism</topic><topic>Sulfhydryl Compounds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ro, Kyung Won</creatorcontrib><creatorcontrib>Hahn, Jong Hoon</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ro, Kyung Won</au><au>Hahn, Jong Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Precolumn diastereomerization and micellar electrokinetic chromatography on a plastic microchip: Rapid chiral analysis of amino acids</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>26</volume><issue>24</issue><spage>4767</spage><epage>4773</epage><pages>4767-4773</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Precolumn derivatization and chiral separation of DL‐amino acids based on diastereomerization have been performed on an integrated poly(dimethylsiloxane) microchip. Diastereomeric derivatives were formed in a microfabricated precolumn reactor by the reaction of amino acid enantiomers with o‐phthaldialdehyde/2,3,4,6‐tetra‐O‐acetyl‐1‐thio‐β‐D‐glucopyranose (OPA/TATG), and separated by MEKC in an achiral environment without chiral selectors in the running buffer. Optimized precolumn reactions and chiral separations of amino acids were achieved within 2.5 min. Resolutions of diastereomers of OPA/TATG‐amino acids were in the range of 2.5–6.1 at optimized separation conditions. Simultaneous separation of a mixture of five chiral amino acids was successfully performed in a single run in less than 100 s.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>16278921</pmid><doi>10.1002/elps.200500370</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0173-0835 |
| ispartof | Electrophoresis, 2005-12, Vol.26 (24), p.4767-4773 |
| issn | 0173-0835 1522-2683 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino acids Amino Acids - analysis Amino Acids - isolation & purification Chiral analysis Chromatography, Micellar Electrokinetic Capillary - methods Diastereomerization Dimethylpolysiloxanes Electrophoresis, Microchip - methods Glucose - analogs & derivatives Glucose - chemistry Miniaturization o-Phthalaldehyde - chemistry Plastic microchip Precolumn reaction Silicones Stereoisomerism Sulfhydryl Compounds - chemistry |
| title | Precolumn diastereomerization and micellar electrokinetic chromatography on a plastic microchip: Rapid chiral analysis of amino acids |
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