Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis
The characterization of protein expression from a single-cell mouse embryo using two-dimensional capillary electrophoresis (2D-CE) is described. These zygotes were obtained from Hsf1 gene knockout mice. Single zygotes were lysed off-column and proteins were fluorescently labeled using the fluorogeni...
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| Veröffentlicht in: | Journal of Chromatography 2006-10, Vol.1130 (2), p.190-194 |
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| container_title | Journal of Chromatography |
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| creator | Harwood, Melissa M. Christians, Elisabeth S. Fazal, Md. Abul Dovichi, Norman J. |
| description | The characterization of protein expression from a single-cell mouse embryo using two-dimensional capillary electrophoresis (2D-CE) is described. These zygotes were obtained from
Hsf1 gene knockout mice. Single zygotes were lysed off-column and proteins were fluorescently labeled using the fluorogenic dye 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ). After injection, analytes were separated first according to molecular weight using capillary sieving electrophoresis (CSE) and then by micellar electrokinetic capillary chromatography (MEKC) to obtain protein expression fingerprints. Analytes were detected in a sheath flow cuvette using laser-induced fluorescence. In a 1-h 2D-CE separation, over 100 components were resolved with a spot capacity of 380. |
| doi_str_mv | 10.1016/j.chroma.2006.05.052 |
| format | Article |
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Hsf1 gene knockout mice. Single zygotes were lysed off-column and proteins were fluorescently labeled using the fluorogenic dye 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ). After injection, analytes were separated first according to molecular weight using capillary sieving electrophoresis (CSE) and then by micellar electrokinetic capillary chromatography (MEKC) to obtain protein expression fingerprints. Analytes were detected in a sheath flow cuvette using laser-induced fluorescence. In a 1-h 2D-CE separation, over 100 components were resolved with a spot capacity of 380.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2006.05.052</identifier><identifier>PMID: 16782116</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Electrophoresis, Capillary ; Electrophoresis, Capillary - methods ; Electrophoresis, Gel, Two-Dimensional ; Electrophoresis, Gel, Two-Dimensional - methods ; Embryo ; Embryo, Mammalian - chemistry ; Embryo, Mammalian - cytology ; Embryo, Mammalian - metabolism ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; Life Sciences ; Mice ; Mice, Knockout ; Proteins ; Proteins - analysis ; Reproducibility of Results ; Single cell analysis ; Single zygote analysis ; Two-dimensional capillary electrophoresis ; Zygote ; Zygote - chemistry ; Zygote - metabolism</subject><ispartof>Journal of Chromatography, 2006-10, Vol.1130 (2), p.190-194</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-f09403a38685cc5e780a73ba7067e709fb1c1b428016aeeb8e5f4cca84a2f6973</citedby><cites>FETCH-LOGICAL-c424t-f09403a38685cc5e780a73ba7067e709fb1c1b428016aeeb8e5f4cca84a2f6973</cites><orcidid>0000-0003-2728-7067</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2006.05.052$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>310,311,315,781,785,790,791,886,3551,23935,23936,25145,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18203684$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16782116$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00170675$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Harwood, Melissa M.</creatorcontrib><creatorcontrib>Christians, Elisabeth S.</creatorcontrib><creatorcontrib>Fazal, Md. Abul</creatorcontrib><creatorcontrib>Dovichi, Norman J.</creatorcontrib><title>Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis</title><title>Journal of Chromatography</title><addtitle>J Chromatogr A</addtitle><description>The characterization of protein expression from a single-cell mouse embryo using two-dimensional capillary electrophoresis (2D-CE) is described. These zygotes were obtained from
Hsf1 gene knockout mice. Single zygotes were lysed off-column and proteins were fluorescently labeled using the fluorogenic dye 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ). After injection, analytes were separated first according to molecular weight using capillary sieving electrophoresis (CSE) and then by micellar electrokinetic capillary chromatography (MEKC) to obtain protein expression fingerprints. Analytes were detected in a sheath flow cuvette using laser-induced fluorescence. In a 1-h 2D-CE separation, over 100 components were resolved with a spot capacity of 380.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Capillary</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Electrophoresis, Gel, Two-Dimensional - methods</subject><subject>Embryo</subject><subject>Embryo, Mammalian - chemistry</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Proteins</subject><subject>Proteins - analysis</subject><subject>Reproducibility of Results</subject><subject>Single cell analysis</subject><subject>Single zygote analysis</subject><subject>Two-dimensional capillary electrophoresis</subject><subject>Zygote</subject><subject>Zygote - chemistry</subject><subject>Zygote - metabolism</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGKFDEQhnNQ3HX1DURyUfDQYyXdnaQvwrKoKwx4UM-hOlPtZEh3xqRnl3l7M9vD7m2hIBC--qn_Y-ydgJUAoT7vVm6b4ogrCaBW0JaRL9glgBRVp3R9wV7nvAMQGrR8xS6E0kYKoS5Z_8tPfwNVjkLg-xRn8hPHCcMx-8zjwJHnB4KP8ZCJ09inY-T9kc_3sdr4kabsY-G5w70PAdORUyA3p7jfxkQl5Q17OWDI9Pb8XrE_377-vrmt1j-__7i5Xleukc1cDdA1UGNtlGmda0kbQF33qEFp0tANvXCib6QphZGoN9QOjXNoGpSD6nR9xT4tuVsMdp_8WG6xEb29vV7b099Df6XbO1HYjwtbKv87UJ7t6PPJAU5UelplOiG0VgVsFtClmHOi4TFZgD25tzu7uLcn9xbaMrKsvT_nH_qRNk9LZ_EF-HAGMDsMQ8LJ-fzEGQm1Mk3hviwcFXN3npLNztPkaONTsWw30T9_yX8DeabA</recordid><startdate>20061020</startdate><enddate>20061020</enddate><creator>Harwood, Melissa M.</creator><creator>Christians, Elisabeth S.</creator><creator>Fazal, Md. Abul</creator><creator>Dovichi, Norman J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2728-7067</orcidid></search><sort><creationdate>20061020</creationdate><title>Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis</title><author>Harwood, Melissa M. ; Christians, Elisabeth S. ; Fazal, Md. Abul ; Dovichi, Norman J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-f09403a38685cc5e780a73ba7067e709fb1c1b428016aeeb8e5f4cca84a2f6973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis, Capillary</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Electrophoresis, Gel, Two-Dimensional - methods</topic><topic>Embryo</topic><topic>Embryo, Mammalian - chemistry</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Proteins</topic><topic>Proteins - analysis</topic><topic>Reproducibility of Results</topic><topic>Single cell analysis</topic><topic>Single zygote analysis</topic><topic>Two-dimensional capillary electrophoresis</topic><topic>Zygote</topic><topic>Zygote - chemistry</topic><topic>Zygote - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harwood, Melissa M.</creatorcontrib><creatorcontrib>Christians, Elisabeth S.</creatorcontrib><creatorcontrib>Fazal, Md. 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Abul</au><au>Dovichi, Norman J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis</atitle><jtitle>Journal of Chromatography</jtitle><addtitle>J Chromatogr A</addtitle><date>2006-10-20</date><risdate>2006</risdate><volume>1130</volume><issue>2</issue><spage>190</spage><epage>194</epage><pages>190-194</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>The characterization of protein expression from a single-cell mouse embryo using two-dimensional capillary electrophoresis (2D-CE) is described. These zygotes were obtained from
Hsf1 gene knockout mice. Single zygotes were lysed off-column and proteins were fluorescently labeled using the fluorogenic dye 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ). After injection, analytes were separated first according to molecular weight using capillary sieving electrophoresis (CSE) and then by micellar electrokinetic capillary chromatography (MEKC) to obtain protein expression fingerprints. Analytes were detected in a sheath flow cuvette using laser-induced fluorescence. In a 1-h 2D-CE separation, over 100 components were resolved with a spot capacity of 380.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16782116</pmid><doi>10.1016/j.chroma.2006.05.052</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0003-2728-7067</orcidid></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Animals Biochemistry, Molecular Biology Biological and medical sciences Electrophoresis, Capillary Electrophoresis, Capillary - methods Electrophoresis, Gel, Two-Dimensional Electrophoresis, Gel, Two-Dimensional - methods Embryo Embryo, Mammalian - chemistry Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Fundamental and applied biological sciences. Psychology General aspects, investigation methods Life Sciences Mice Mice, Knockout Proteins Proteins - analysis Reproducibility of Results Single cell analysis Single zygote analysis Two-dimensional capillary electrophoresis Zygote Zygote - chemistry Zygote - metabolism |
| title | Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis |
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