$Analysis of differential detergent fractions of an AtT-20 cellular homogenate using one- and two-dimensional capillary electrophoresis$

Analysis of differential detergent fractions of an AtT-20 cellular homogenate using one- and two-dimensional capillary electrophoresis

Differential detergent fractionation was used to sequentially extract cytosolic, membrane, nuclear, and cytoskeletal fractions from AtT-20 cells. Extracted components were denatured by sodium dodecyl sulfate (SDS) and then labeled with the fluorogenic reagent 3-(2-furoyl) quinoline-1-carboxaldehyde....

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Veröffentlicht in:	Journal of Chromatography A 2006-10, Vol.1130 (2), p.182-189
Hauptverfasser:	Fazal, Md. Abul, Palmer, Vanessa R., Dovichi, Norman J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cells, Cultured Chromatography, Micellar Electrokinetic Capillary - methods Detergents - chemistry Differential detergent fractionation Electrophoresis, Capillary - methods Electrophoresis, Gel, Two-Dimensional - methods Electrophoresis, Polyacrylamide Gel - methods Fundamental and applied biological sciences. Psychology General aspects, investigation methods Humans Mice Proteins Proteins - analysis Two-dimensional capillary electrophoresis
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