Fluorescence in situ hybridization studies using BAC clones of the EVI1 locus in hematological malignancies with 3q rearrangements
Chromosomal rearrangements involving 3q26 are a recurrent aberration in malignant myeloid disorders. Several of these rearrangements involve the EVI1 oncogene or its surrounding sequences and are associated with a poor prognosis. Fluorescence in situ hybridization (FISH) studies using bacterial arti...
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| Veröffentlicht in: | Cancer genetics and cytogenetics 2006-10, Vol.170 (2), p.115-120 |
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| creator | Madrigal, Irene Carrió, Ana Gómez, Cándida Rozman, María Esteve, Jordi Nomdedeu, Benet Campo, Elías Costa, Dolors |
| description | Chromosomal rearrangements involving 3q26 are a recurrent aberration in malignant myeloid disorders. Several of these rearrangements involve the
EVI1 oncogene or its surrounding sequences and are associated with a poor prognosis. Fluorescence in situ hybridization (FISH) studies using bacterial artificial chromosome (BAC) clones were conducted to determine whether the
EVI1 locus was rearranged in nine patients with hematological malignancies carrying 3q abnormalities. A dual-color probe was constructed with nine BACs; centromeric clones covering 1 Mb and including the
EVI1 gene were labeled with a red fluorescent dye and telomeric clones covering 1 Mb were labeled with a green fluorescent dye. Two patients showed normal copies of the
EVI1 locus, four patients showed one
EVI1 locus rearranged (in all of them the breakpoint on 3q26 was telomeric to
EVI1), one patient showed one copy of the
EVI1 locus translocated to another chromosome, one patient showed one copy of the
EVI1 locus rearranged and the other copy translocated, and one patient showed one extra copy of the
EVI1 locus. In four cases, FISH studies using the
EVI1 clones detected different 3q abnormalities not previously found by conventional cytogenetics. FISH analysis with BAC clones was a useful tool for identifying the chromosome breakpoints affecting the
EVI1 locus in patients with 3q26 rearrangements. |
| doi_str_mv | 10.1016/j.cancergencyto.2006.05.007 |
| format | Article |
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EVI1 oncogene or its surrounding sequences and are associated with a poor prognosis. Fluorescence in situ hybridization (FISH) studies using bacterial artificial chromosome (BAC) clones were conducted to determine whether the
EVI1 locus was rearranged in nine patients with hematological malignancies carrying 3q abnormalities. A dual-color probe was constructed with nine BACs; centromeric clones covering 1 Mb and including the
EVI1 gene were labeled with a red fluorescent dye and telomeric clones covering 1 Mb were labeled with a green fluorescent dye. Two patients showed normal copies of the
EVI1 locus, four patients showed one
EVI1 locus rearranged (in all of them the breakpoint on 3q26 was telomeric to
EVI1), one patient showed one copy of the
EVI1 locus translocated to another chromosome, one patient showed one copy of the
EVI1 locus rearranged and the other copy translocated, and one patient showed one extra copy of the
EVI1 locus. In four cases, FISH studies using the
EVI1 clones detected different 3q abnormalities not previously found by conventional cytogenetics. FISH analysis with BAC clones was a useful tool for identifying the chromosome breakpoints affecting the
EVI1 locus in patients with 3q26 rearrangements.</description><identifier>ISSN: 0165-4608</identifier><identifier>EISSN: 1873-4456</identifier><identifier>DOI: 10.1016/j.cancergencyto.2006.05.007</identifier><identifier>PMID: 17011981</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Chromosome Aberrations ; Chromosomes, Artificial, Bacterial ; Chromosomes, Human, Pair 3 ; DNA-Binding Proteins - genetics ; Gene Duplication ; Hematologic Neoplasms - genetics ; Humans ; In Situ Hybridization, Fluorescence ; MDS1 and EVI1 Complex Locus Protein ; Proto-Oncogenes - genetics ; Transcription Factors - genetics ; Translocation, Genetic</subject><ispartof>Cancer genetics and cytogenetics, 2006-10, Vol.170 (2), p.115-120</ispartof><rights>2006 Elsevier Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-5c344be59b4af188c7eb94541eb2bc639ae66c114b42e5199820ea38cd39e4cf3</citedby><cites>FETCH-LOGICAL-c412t-5c344be59b4af188c7eb94541eb2bc639ae66c114b42e5199820ea38cd39e4cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cancergencyto.2006.05.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17011981$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Madrigal, Irene</creatorcontrib><creatorcontrib>Carrió, Ana</creatorcontrib><creatorcontrib>Gómez, Cándida</creatorcontrib><creatorcontrib>Rozman, María</creatorcontrib><creatorcontrib>Esteve, Jordi</creatorcontrib><creatorcontrib>Nomdedeu, Benet</creatorcontrib><creatorcontrib>Campo, Elías</creatorcontrib><creatorcontrib>Costa, Dolors</creatorcontrib><title>Fluorescence in situ hybridization studies using BAC clones of the EVI1 locus in hematological malignancies with 3q rearrangements</title><title>Cancer genetics and cytogenetics</title><addtitle>Cancer Genet Cytogenet</addtitle><description>Chromosomal rearrangements involving 3q26 are a recurrent aberration in malignant myeloid disorders. Several of these rearrangements involve the
EVI1 oncogene or its surrounding sequences and are associated with a poor prognosis. Fluorescence in situ hybridization (FISH) studies using bacterial artificial chromosome (BAC) clones were conducted to determine whether the
EVI1 locus was rearranged in nine patients with hematological malignancies carrying 3q abnormalities. A dual-color probe was constructed with nine BACs; centromeric clones covering 1 Mb and including the
EVI1 gene were labeled with a red fluorescent dye and telomeric clones covering 1 Mb were labeled with a green fluorescent dye. Two patients showed normal copies of the
EVI1 locus, four patients showed one
EVI1 locus rearranged (in all of them the breakpoint on 3q26 was telomeric to
EVI1), one patient showed one copy of the
EVI1 locus translocated to another chromosome, one patient showed one copy of the
EVI1 locus rearranged and the other copy translocated, and one patient showed one extra copy of the
EVI1 locus. In four cases, FISH studies using the
EVI1 clones detected different 3q abnormalities not previously found by conventional cytogenetics. FISH analysis with BAC clones was a useful tool for identifying the chromosome breakpoints affecting the
EVI1 locus in patients with 3q26 rearrangements.</description><subject>Chromosome Aberrations</subject><subject>Chromosomes, Artificial, Bacterial</subject><subject>Chromosomes, Human, Pair 3</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Gene Duplication</subject><subject>Hematologic Neoplasms - genetics</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>MDS1 and EVI1 Complex Locus Protein</subject><subject>Proto-Oncogenes - genetics</subject><subject>Transcription Factors - genetics</subject><subject>Translocation, Genetic</subject><issn>0165-4608</issn><issn>1873-4456</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUGL1DAUx4Mo7rj6FSQgeGtN0iRt8bQOs7qw4EW9hjR97WRIk90kVcajn9wMMyCe9BR4_N7v5b0_Qm8oqSmh8t2hNtobiDN4c8yhZoTImoiakPYJ2tCubSrOhXyKNoUWFZeku0IvUjqQQrBePkdXtCWU9h3doF-3bg0RkikywNbjZPOK98ch2tH-1NmGUsrraCHhNVk_4w83W2xc8KUQJpz3gHff7ih2wazpJNjDonNwYbZGO7xoZ2df_nsS_LB5j5tHHEHHqP0MC_icXqJnk3YJXl3ea_T1dvdl-6m6__zxbntzXxlOWa6EaTgfQPQD1xPtOtPC0HPBKQxsMLLpNUhpKOUDZyBo33eMgG46MzY9cDM11-jt2fsQw-MKKavFlr2d0x7CmpTsetK2QvwTZIS1jLW8gO_PoIkhpQiTeoh20fGoKFGnrNRB_ZWVOmWliFAlidL9-jJmHRYY__RewinA7gxAucp3C1GlcsaiG20Ek9UY7H8N-g20u6-H</recordid><startdate>20061015</startdate><enddate>20061015</enddate><creator>Madrigal, Irene</creator><creator>Carrió, Ana</creator><creator>Gómez, Cándida</creator><creator>Rozman, María</creator><creator>Esteve, Jordi</creator><creator>Nomdedeu, Benet</creator><creator>Campo, Elías</creator><creator>Costa, Dolors</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061015</creationdate><title>Fluorescence in situ hybridization studies using BAC clones of the EVI1 locus in hematological malignancies with 3q rearrangements</title><author>Madrigal, Irene ; Carrió, Ana ; Gómez, Cándida ; Rozman, María ; Esteve, Jordi ; Nomdedeu, Benet ; Campo, Elías ; Costa, Dolors</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-5c344be59b4af188c7eb94541eb2bc639ae66c114b42e5199820ea38cd39e4cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Chromosome Aberrations</topic><topic>Chromosomes, Artificial, Bacterial</topic><topic>Chromosomes, Human, Pair 3</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Gene Duplication</topic><topic>Hematologic Neoplasms - genetics</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>MDS1 and EVI1 Complex Locus Protein</topic><topic>Proto-Oncogenes - genetics</topic><topic>Transcription Factors - genetics</topic><topic>Translocation, Genetic</topic><toplevel>online_resources</toplevel><creatorcontrib>Madrigal, Irene</creatorcontrib><creatorcontrib>Carrió, Ana</creatorcontrib><creatorcontrib>Gómez, Cándida</creatorcontrib><creatorcontrib>Rozman, María</creatorcontrib><creatorcontrib>Esteve, Jordi</creatorcontrib><creatorcontrib>Nomdedeu, Benet</creatorcontrib><creatorcontrib>Campo, Elías</creatorcontrib><creatorcontrib>Costa, Dolors</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer genetics and cytogenetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Madrigal, Irene</au><au>Carrió, Ana</au><au>Gómez, Cándida</au><au>Rozman, María</au><au>Esteve, Jordi</au><au>Nomdedeu, Benet</au><au>Campo, Elías</au><au>Costa, Dolors</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescence in situ hybridization studies using BAC clones of the EVI1 locus in hematological malignancies with 3q rearrangements</atitle><jtitle>Cancer genetics and cytogenetics</jtitle><addtitle>Cancer Genet Cytogenet</addtitle><date>2006-10-15</date><risdate>2006</risdate><volume>170</volume><issue>2</issue><spage>115</spage><epage>120</epage><pages>115-120</pages><issn>0165-4608</issn><eissn>1873-4456</eissn><abstract>Chromosomal rearrangements involving 3q26 are a recurrent aberration in malignant myeloid disorders. Several of these rearrangements involve the
EVI1 oncogene or its surrounding sequences and are associated with a poor prognosis. Fluorescence in situ hybridization (FISH) studies using bacterial artificial chromosome (BAC) clones were conducted to determine whether the
EVI1 locus was rearranged in nine patients with hematological malignancies carrying 3q abnormalities. A dual-color probe was constructed with nine BACs; centromeric clones covering 1 Mb and including the
EVI1 gene were labeled with a red fluorescent dye and telomeric clones covering 1 Mb were labeled with a green fluorescent dye. Two patients showed normal copies of the
EVI1 locus, four patients showed one
EVI1 locus rearranged (in all of them the breakpoint on 3q26 was telomeric to
EVI1), one patient showed one copy of the
EVI1 locus translocated to another chromosome, one patient showed one copy of the
EVI1 locus rearranged and the other copy translocated, and one patient showed one extra copy of the
EVI1 locus. In four cases, FISH studies using the
EVI1 clones detected different 3q abnormalities not previously found by conventional cytogenetics. FISH analysis with BAC clones was a useful tool for identifying the chromosome breakpoints affecting the
EVI1 locus in patients with 3q26 rearrangements.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17011981</pmid><doi>10.1016/j.cancergencyto.2006.05.007</doi><tpages>6</tpages></addata></record> |
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| subjects | Chromosome Aberrations Chromosomes, Artificial, Bacterial Chromosomes, Human, Pair 3 DNA-Binding Proteins - genetics Gene Duplication Hematologic Neoplasms - genetics Humans In Situ Hybridization, Fluorescence MDS1 and EVI1 Complex Locus Protein Proto-Oncogenes - genetics Transcription Factors - genetics Translocation, Genetic |
| title | Fluorescence in situ hybridization studies using BAC clones of the EVI1 locus in hematological malignancies with 3q rearrangements |
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