Sex Steroids Are Involved in the Regulation of Gonadotropin-Releasing Hormone and Dopamine D2 Receptors in Female Tilapia Pituitary
Although molecular mechanisms underlying steroid effects on GnRH and dopamine receptors are well documented in mammals, little is known in fish. Herein, we describe the expression of pituitary GnRH and dopamine receptors relative to gonadotropin expression and release. We exposed female tilapia to g...
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| Veröffentlicht in: | Biology of reproduction 2006-10, Vol.75 (4), p.642-650 |
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| creator | Levavi-Sivan, Berta Biran, Jakob Fireman, Einat |
| description | Although
molecular mechanisms underlying steroid effects on GnRH and dopamine receptors are well documented in mammals, little is known
in fish. Herein, we describe the expression of pituitary GnRH and dopamine receptors relative to gonadotropin expression and
release. We exposed female tilapia to graded doses of estradiol or 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) in vitro,
and of estradiol in vivo, and determined mRNA levels of
gnrhr1 ,
gnrhr3 ,
drd2 ,
lhb , and
fshb
by real-time PCR. We also determined gonadotropin levels using specific ELISAs. Exposure to low doses of estradiol caused
increased
gnrhr3
mRNA levels in vivo and in vitro, probably related to positive feedback on FSH release. Increasing concentrations of estradiol
resulted in increased
drd2
mRNA levels in vivo and in vitro, inhibition of LH and FSH release, and inhibition of
lhb
mRNA levels
in vivo, possibly related to negative feedback. At high doses of estradiol, FSH release increased in preparation for a new
generation of follicles. Exposure to nanomolar doses of DHP resulted in increased
drd2
mRNA levels, probably related to negative feedback on LH release. A decrease in
drd2
levels at the micromolar range of DHP (concomitant with increased
gnrhr3
and
fshb
mRNA levels) may be related to the recruitment of a new generation of oocytes. Exposure to DHP also resulted in increased
lhb
mRNA levels toward final oocyte maturation. Salmon GnRH analog (sGnRHa) increased mRNA levels of
gnrh1
and
gnrh3 ; when combined with DHP, sGnRHa synergistically increased expression of
gnrh3
only. These results emphasize the role of sex steroids on positive and negative feedbacks controlling the reproductive cycle.
Abstract
Estradiol and 17α,20β-dihydroxy-4-pregnen-3-one are implicated in the synthesis of GnRH and dopamine receptors in tilapia
female, in relation to gonadotropin expression and release |
| doi_str_mv | 10.1095/biolreprod.106.051540 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_68907256</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68907256</sourcerecordid><originalsourceid>FETCH-LOGICAL-h300t-faa86e5ba84581997b3d9905d69ffb98e5a9e5b24763272beda81f9f335568e43</originalsourceid><addsrcrecordid>eNqFkE1v1DAQhi0EokvLTwD5ArcUf8SOfaxa-iFVomrLOZpsJrtGjh3spNue-8drxCK4cRq9mmdeaR5CPnB2zJlVXzoXfcIpxb5kfcwUVzV7RVZcCVs1QpvXZMUY05WUWh6Qdzn_YIzXUsi35IBrwxppxIo83-EjvZsxRddnepKQXoWH6B-wpy7QeYv0FjeLh9nFQONAL2KAPs4pTi5Ut-gRsgsbehnTGANSCD09ixOMroQzUY7XOM0x5V9t5ziCR3rvPEwO6I2bFzdDejoibwbwGd_v5yH5fv71_vSyuv52cXV6cl1tJWNzNQAYjaoDUyvDrW062VvLVK_tMHTWoAJb1qJutBSN6LAHwwc7SKmUNljLQ_L5d2-x9nPBPLejy2v0HgLGJbfaWNYIpf8LclskC6UK-HEPLt2IfTslN5aH2j9-C_BpD0Begx8ShLXLfznDjWb1P9zWbbY7l7DNxZUvtbLd7XaNautWF-4Fm-yaag</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19529255</pqid></control><display><type>article</type><title>Sex Steroids Are Involved in the Regulation of Gonadotropin-Releasing Hormone and Dopamine D2 Receptors in Female Tilapia Pituitary</title><source>MEDLINE</source><source>BioOne Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Levavi-Sivan, Berta ; Biran, Jakob ; Fireman, Einat</creator><creatorcontrib>Levavi-Sivan, Berta ; Biran, Jakob ; Fireman, Einat</creatorcontrib><description>Although
molecular mechanisms underlying steroid effects on GnRH and dopamine receptors are well documented in mammals, little is known
in fish. Herein, we describe the expression of pituitary GnRH and dopamine receptors relative to gonadotropin expression and
release. We exposed female tilapia to graded doses of estradiol or 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) in vitro,
and of estradiol in vivo, and determined mRNA levels of
gnrhr1 ,
gnrhr3 ,
drd2 ,
lhb , and
fshb
by real-time PCR. We also determined gonadotropin levels using specific ELISAs. Exposure to low doses of estradiol caused
increased
gnrhr3
mRNA levels in vivo and in vitro, probably related to positive feedback on FSH release. Increasing concentrations of estradiol
resulted in increased
drd2
mRNA levels in vivo and in vitro, inhibition of LH and FSH release, and inhibition of
lhb
mRNA levels
in vivo, possibly related to negative feedback. At high doses of estradiol, FSH release increased in preparation for a new
generation of follicles. Exposure to nanomolar doses of DHP resulted in increased
drd2
mRNA levels, probably related to negative feedback on LH release. A decrease in
drd2
levels at the micromolar range of DHP (concomitant with increased
gnrhr3
and
fshb
mRNA levels) may be related to the recruitment of a new generation of oocytes. Exposure to DHP also resulted in increased
lhb
mRNA levels toward final oocyte maturation. Salmon GnRH analog (sGnRHa) increased mRNA levels of
gnrh1
and
gnrh3 ; when combined with DHP, sGnRHa synergistically increased expression of
gnrh3
only. These results emphasize the role of sex steroids on positive and negative feedbacks controlling the reproductive cycle.
Abstract
Estradiol and 17α,20β-dihydroxy-4-pregnen-3-one are implicated in the synthesis of GnRH and dopamine receptors in tilapia
female, in relation to gonadotropin expression and release</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.106.051540</identifier><identifier>PMID: 16807382</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Dose-Response Relationship, Drug ; Estradiol - metabolism ; Estradiol - pharmacology ; Female ; Follicle Stimulating Hormone - metabolism ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Gonadal Steroid Hormones - metabolism ; Gonadal Steroid Hormones - pharmacology ; Gonadotropin-Releasing Hormone - metabolism ; Gonadotropin-Releasing Hormone - pharmacology ; Hormones and neuropeptides. Regulation ; Hydroxyprogesterones - metabolism ; Hydroxyprogesterones - pharmacology ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Pituitary Gland - drug effects ; Pituitary Gland - metabolism ; Receptors, Dopamine D2 - drug effects ; Receptors, Dopamine D2 - genetics ; Receptors, Dopamine D2 - metabolism ; Receptors, LHRH - drug effects ; Receptors, LHRH - genetics ; Receptors, LHRH - metabolism ; Salmonidae ; Steroids - metabolism ; Steroids - pharmacology ; Tilapia ; Tilapia - physiology ; Vertebrates: endocrinology ; Vertebrates: reproduction ; Vitellogenesis - physiology</subject><ispartof>Biology of reproduction, 2006-10, Vol.75 (4), p.642-650</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18186042$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16807382$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Levavi-Sivan, Berta</creatorcontrib><creatorcontrib>Biran, Jakob</creatorcontrib><creatorcontrib>Fireman, Einat</creatorcontrib><title>Sex Steroids Are Involved in the Regulation of Gonadotropin-Releasing Hormone and Dopamine D2 Receptors in Female Tilapia Pituitary</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Although
molecular mechanisms underlying steroid effects on GnRH and dopamine receptors are well documented in mammals, little is known
in fish. Herein, we describe the expression of pituitary GnRH and dopamine receptors relative to gonadotropin expression and
release. We exposed female tilapia to graded doses of estradiol or 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) in vitro,
and of estradiol in vivo, and determined mRNA levels of
gnrhr1 ,
gnrhr3 ,
drd2 ,
lhb , and
fshb
by real-time PCR. We also determined gonadotropin levels using specific ELISAs. Exposure to low doses of estradiol caused
increased
gnrhr3
mRNA levels in vivo and in vitro, probably related to positive feedback on FSH release. Increasing concentrations of estradiol
resulted in increased
drd2
mRNA levels in vivo and in vitro, inhibition of LH and FSH release, and inhibition of
lhb
mRNA levels
in vivo, possibly related to negative feedback. At high doses of estradiol, FSH release increased in preparation for a new
generation of follicles. Exposure to nanomolar doses of DHP resulted in increased
drd2
mRNA levels, probably related to negative feedback on LH release. A decrease in
drd2
levels at the micromolar range of DHP (concomitant with increased
gnrhr3
and
fshb
mRNA levels) may be related to the recruitment of a new generation of oocytes. Exposure to DHP also resulted in increased
lhb
mRNA levels toward final oocyte maturation. Salmon GnRH analog (sGnRHa) increased mRNA levels of
gnrh1
and
gnrh3 ; when combined with DHP, sGnRHa synergistically increased expression of
gnrh3
only. These results emphasize the role of sex steroids on positive and negative feedbacks controlling the reproductive cycle.
Abstract
Estradiol and 17α,20β-dihydroxy-4-pregnen-3-one are implicated in the synthesis of GnRH and dopamine receptors in tilapia
female, in relation to gonadotropin expression and release</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Dose-Response Relationship, Drug</subject><subject>Estradiol - metabolism</subject><subject>Estradiol - pharmacology</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - metabolism</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Gonadal Steroid Hormones - metabolism</subject><subject>Gonadal Steroid Hormones - pharmacology</subject><subject>Gonadotropin-Releasing Hormone - metabolism</subject><subject>Gonadotropin-Releasing Hormone - pharmacology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hydroxyprogesterones - metabolism</subject><subject>Hydroxyprogesterones - pharmacology</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Pituitary Gland - drug effects</subject><subject>Pituitary Gland - metabolism</subject><subject>Receptors, Dopamine D2 - drug effects</subject><subject>Receptors, Dopamine D2 - genetics</subject><subject>Receptors, Dopamine D2 - metabolism</subject><subject>Receptors, LHRH - drug effects</subject><subject>Receptors, LHRH - genetics</subject><subject>Receptors, LHRH - metabolism</subject><subject>Salmonidae</subject><subject>Steroids - metabolism</subject><subject>Steroids - pharmacology</subject><subject>Tilapia</subject><subject>Tilapia - physiology</subject><subject>Vertebrates: endocrinology</subject><subject>Vertebrates: reproduction</subject><subject>Vitellogenesis - physiology</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvLTwD5ArcUf8SOfaxa-iFVomrLOZpsJrtGjh3spNue-8drxCK4cRq9mmdeaR5CPnB2zJlVXzoXfcIpxb5kfcwUVzV7RVZcCVs1QpvXZMUY05WUWh6Qdzn_YIzXUsi35IBrwxppxIo83-EjvZsxRddnepKQXoWH6B-wpy7QeYv0FjeLh9nFQONAL2KAPs4pTi5Ut-gRsgsbehnTGANSCD09ixOMroQzUY7XOM0x5V9t5ziCR3rvPEwO6I2bFzdDejoibwbwGd_v5yH5fv71_vSyuv52cXV6cl1tJWNzNQAYjaoDUyvDrW062VvLVK_tMHTWoAJb1qJutBSN6LAHwwc7SKmUNljLQ_L5d2-x9nPBPLejy2v0HgLGJbfaWNYIpf8LclskC6UK-HEPLt2IfTslN5aH2j9-C_BpD0Begx8ShLXLfznDjWb1P9zWbbY7l7DNxZUvtbLd7XaNautWF-4Fm-yaag</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Levavi-Sivan, Berta</creator><creator>Biran, Jakob</creator><creator>Fireman, Einat</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>Sex Steroids Are Involved in the Regulation of Gonadotropin-Releasing Hormone and Dopamine D2 Receptors in Female Tilapia Pituitary</title><author>Levavi-Sivan, Berta ; Biran, Jakob ; Fireman, Einat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h300t-faa86e5ba84581997b3d9905d69ffb98e5a9e5b24763272beda81f9f335568e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Dose-Response Relationship, Drug</topic><topic>Estradiol - metabolism</topic><topic>Estradiol - pharmacology</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - metabolism</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Gonadal Steroid Hormones - metabolism</topic><topic>Gonadal Steroid Hormones - pharmacology</topic><topic>Gonadotropin-Releasing Hormone - metabolism</topic><topic>Gonadotropin-Releasing Hormone - pharmacology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hydroxyprogesterones - metabolism</topic><topic>Hydroxyprogesterones - pharmacology</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Pituitary Gland - drug effects</topic><topic>Pituitary Gland - metabolism</topic><topic>Receptors, Dopamine D2 - drug effects</topic><topic>Receptors, Dopamine D2 - genetics</topic><topic>Receptors, Dopamine D2 - metabolism</topic><topic>Receptors, LHRH - drug effects</topic><topic>Receptors, LHRH - genetics</topic><topic>Receptors, LHRH - metabolism</topic><topic>Salmonidae</topic><topic>Steroids - metabolism</topic><topic>Steroids - pharmacology</topic><topic>Tilapia</topic><topic>Tilapia - physiology</topic><topic>Vertebrates: endocrinology</topic><topic>Vertebrates: reproduction</topic><topic>Vitellogenesis - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Levavi-Sivan, Berta</creatorcontrib><creatorcontrib>Biran, Jakob</creatorcontrib><creatorcontrib>Fireman, Einat</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Levavi-Sivan, Berta</au><au>Biran, Jakob</au><au>Fireman, Einat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sex Steroids Are Involved in the Regulation of Gonadotropin-Releasing Hormone and Dopamine D2 Receptors in Female Tilapia Pituitary</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>75</volume><issue>4</issue><spage>642</spage><epage>650</epage><pages>642-650</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Although
molecular mechanisms underlying steroid effects on GnRH and dopamine receptors are well documented in mammals, little is known
in fish. Herein, we describe the expression of pituitary GnRH and dopamine receptors relative to gonadotropin expression and
release. We exposed female tilapia to graded doses of estradiol or 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) in vitro,
and of estradiol in vivo, and determined mRNA levels of
gnrhr1 ,
gnrhr3 ,
drd2 ,
lhb , and
fshb
by real-time PCR. We also determined gonadotropin levels using specific ELISAs. Exposure to low doses of estradiol caused
increased
gnrhr3
mRNA levels in vivo and in vitro, probably related to positive feedback on FSH release. Increasing concentrations of estradiol
resulted in increased
drd2
mRNA levels in vivo and in vitro, inhibition of LH and FSH release, and inhibition of
lhb
mRNA levels
in vivo, possibly related to negative feedback. At high doses of estradiol, FSH release increased in preparation for a new
generation of follicles. Exposure to nanomolar doses of DHP resulted in increased
drd2
mRNA levels, probably related to negative feedback on LH release. A decrease in
drd2
levels at the micromolar range of DHP (concomitant with increased
gnrhr3
and
fshb
mRNA levels) may be related to the recruitment of a new generation of oocytes. Exposure to DHP also resulted in increased
lhb
mRNA levels toward final oocyte maturation. Salmon GnRH analog (sGnRHa) increased mRNA levels of
gnrh1
and
gnrh3 ; when combined with DHP, sGnRHa synergistically increased expression of
gnrh3
only. These results emphasize the role of sex steroids on positive and negative feedbacks controlling the reproductive cycle.
Abstract
Estradiol and 17α,20β-dihydroxy-4-pregnen-3-one are implicated in the synthesis of GnRH and dopamine receptors in tilapia
female, in relation to gonadotropin expression and release</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>16807382</pmid><doi>10.1095/biolreprod.106.051540</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 2006-10, Vol.75 (4), p.642-650 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68907256 |
| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Dose-Response Relationship, Drug Estradiol - metabolism Estradiol - pharmacology Female Follicle Stimulating Hormone - metabolism Freshwater Fundamental and applied biological sciences. Psychology Gene Expression Regulation Gonadal Steroid Hormones - metabolism Gonadal Steroid Hormones - pharmacology Gonadotropin-Releasing Hormone - metabolism Gonadotropin-Releasing Hormone - pharmacology Hormones and neuropeptides. Regulation Hydroxyprogesterones - metabolism Hydroxyprogesterones - pharmacology Hypothalamus. Hypophysis. Epiphysis. Urophysis Pituitary Gland - drug effects Pituitary Gland - metabolism Receptors, Dopamine D2 - drug effects Receptors, Dopamine D2 - genetics Receptors, Dopamine D2 - metabolism Receptors, LHRH - drug effects Receptors, LHRH - genetics Receptors, LHRH - metabolism Salmonidae Steroids - metabolism Steroids - pharmacology Tilapia Tilapia - physiology Vertebrates: endocrinology Vertebrates: reproduction Vitellogenesis - physiology |
| title | Sex Steroids Are Involved in the Regulation of Gonadotropin-Releasing Hormone and Dopamine D2 Receptors in Female Tilapia Pituitary |
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