Proteome mapping of mature pollen of Arabidopsis thaliana

The male gametophyte of Arabidopsis is a three‐celled pollen grain that is thought to contain almost all the mRNAs needed for germination and rapid pollen tube growth. We generated a reference map of the Arabidopsis mature pollen proteome by using multiple protein extraction techniques followed by 2...

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Veröffentlicht in:	Proteomics (Weinheim) 2005-12, Vol.5 (18), p.4864-4884
Hauptverfasser:	Holmes-Davis, Rachel, Tanaka, Charlene K., Vensel, William H., Hurkman, William J., McCormick, Sheila
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis Proteins - analysis Arabidopsis thaliana Biological and medical sciences Electrophoresis, Gel, Two-Dimensional Electrospray ionization tandem mass spectrometry (ESI-MS/MS) Fundamental and applied biological sciences. Psychology Male gametophyte Miscellaneous Molecular Sequence Data Plant Pollen - chemistry Pollen - growth & development Proteins Proteome - analysis Proteomics - methods Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Sequence Alignment Spectrometry, Mass, Electrospray Ionization Two-dimensional gel electrophoresis (2-DE)
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