Transient state kinetics of enzyme IICBGlc, a glucose transporter of the phosphoenolpyruvate phosphotransferase system of Escherichia coli: equilibrium and second order rate constants for the glucose binding and phosphotransfer reactions

During translocation across the cytoplasmic membrane of Escherichia coli, glucose is phosphorylated by phospho-IIA(Glc) and Enzyme IICB(Glc), the last two proteins in the phosphotransfer sequence of the phosphoenolpyruvate:glucose phosphotransferase system. Transient state (rapid quench) methods wer...

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Veröffentlicht in:	The Journal of biological chemistry 2005-12, Vol.280 (51), p.41872-41880
Hauptverfasser:	Meadow, Norman D, Savtchenko, Regina S, Nezami, Azin, Roseman, Saul
Format:	Artikel
Sprache:	eng
Schlagworte:	Enzyme Stability Escherichia coli - enzymology Glucose - metabolism Glucose Transport Proteins, Facilitative - metabolism Kinetics Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism Phosphorylation Protein Binding
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