Endothelin-1 as a central mediator of LPS-induced fever in rats
Fever induced by E. coli lipopolysaccharide (LPS) in rats is substantially reduced by blockade of central endothelin ET B receptors. This study explores the role of endothelin-1 as a central mediator of fever in rats, by investigating the effect of a pyrogenic dose of LPS on the levels of big endoth...
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| Veröffentlicht in: | Brain research 2005-12, Vol.1066 (1), p.92-100 |
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| creator | Fabricio, Aline S.C. Rae, Giles A. D'Orléans-Juste, Pedro Souza, Glória E.P. |
| description | Fever induced by
E. coli lipopolysaccharide (LPS) in rats is substantially reduced by blockade of central endothelin ET
B receptors. This study explores the role of endothelin-1 as a central mediator of fever in rats, by investigating the effect of a pyrogenic dose of LPS on the levels of big endothelin-1 and endothelin-1 in the cerebrospinal fluid (CSF) and endothelin-1 in the plasma. We further assessed whether the increase in body temperature caused by central injection of endothelin-1 constitutes solely a hyperthermia or a true integrated febrile response. LPS (5 μg kg
−1, i.v.) induced fever which peaked at 1.16 ± 0.24 °C within 2 h and remained stable up to 5 h. CSF levels of immunoreactive (ir) big endothelin-1 decreased to undetectable levels at 3 h after LPS, returning only partially at 5 h post-injection. CSF ir-endothelin-1 levels were undetectable in saline-treated animals, but reached 21.9 ± 5.2 fmol ml
−1 at 3 h after LPS treatment. Plasma ir-endothelin-1 levels were unchanged after saline or LPS. Central injection of endothelin-1 (1 pmol, i.c.v.) caused long-lasting increases in body temperature (0.81 ± 0.17 °C, 3 h), but simultaneously decreased tail skin temperature (−1.10 ± 0.26 °C), indicating cutaneous vasoconstriction. Moreover, endothelin-1 induced fever (1.0 ± 0.3 °C, 3 h) when injected into the preoptic area of the anterior hypothalamus (100 fmol), but not i.v. (1 or 10 pmol). These data suggest that endothelin-1 is produced in the brain and acts centrally as a mediator of LPS-induced fever. |
| doi_str_mv | 10.1016/j.brainres.2005.10.037 |
| format | Article |
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E. coli lipopolysaccharide (LPS) in rats is substantially reduced by blockade of central endothelin ET
B receptors. This study explores the role of endothelin-1 as a central mediator of fever in rats, by investigating the effect of a pyrogenic dose of LPS on the levels of big endothelin-1 and endothelin-1 in the cerebrospinal fluid (CSF) and endothelin-1 in the plasma. We further assessed whether the increase in body temperature caused by central injection of endothelin-1 constitutes solely a hyperthermia or a true integrated febrile response. LPS (5 μg kg
−1, i.v.) induced fever which peaked at 1.16 ± 0.24 °C within 2 h and remained stable up to 5 h. CSF levels of immunoreactive (ir) big endothelin-1 decreased to undetectable levels at 3 h after LPS, returning only partially at 5 h post-injection. CSF ir-endothelin-1 levels were undetectable in saline-treated animals, but reached 21.9 ± 5.2 fmol ml
−1 at 3 h after LPS treatment. Plasma ir-endothelin-1 levels were unchanged after saline or LPS. Central injection of endothelin-1 (1 pmol, i.c.v.) caused long-lasting increases in body temperature (0.81 ± 0.17 °C, 3 h), but simultaneously decreased tail skin temperature (−1.10 ± 0.26 °C), indicating cutaneous vasoconstriction. Moreover, endothelin-1 induced fever (1.0 ± 0.3 °C, 3 h) when injected into the preoptic area of the anterior hypothalamus (100 fmol), but not i.v. (1 or 10 pmol). These data suggest that endothelin-1 is produced in the brain and acts centrally as a mediator of LPS-induced fever.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2005.10.037</identifier><identifier>PMID: 16360659</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Antihypertensive Agents - pharmacology ; Big endothelin-1 ; Biological and medical sciences ; Body Temperature - drug effects ; Cerebrospinal fluid ; Endothelin-1 ; Endothelin-1 - cerebrospinal fluid ; Endothelin-1 - metabolism ; Endothelin-1 - pharmacology ; Endothelin-1 - physiology ; Escherichia coli ; Fever ; Fever - chemically induced ; Fever - physiopathology ; Fundamental and applied biological sciences. Psychology ; Hypothalamus ; Injections, Intravenous ; Lipopolysaccharide ; Lipopolysaccharides ; Male ; Microinjections ; Oligopeptides - pharmacology ; Piperidines - pharmacology ; Preoptic area ; Preoptic Area - physiology ; Rats ; Rats, Wistar ; Skin Temperature - drug effects ; Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Brain research, 2005-12, Vol.1066 (1), p.92-100</ispartof><rights>2005</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-251422b7c3d2a7d2d4c6c0662aebdda00d4d264fb88714ba0fd335be824e503a3</citedby><cites>FETCH-LOGICAL-c493t-251422b7c3d2a7d2d4c6c0662aebdda00d4d264fb88714ba0fd335be824e503a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2005.10.037$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17352135$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16360659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fabricio, Aline S.C.</creatorcontrib><creatorcontrib>Rae, Giles A.</creatorcontrib><creatorcontrib>D'Orléans-Juste, Pedro</creatorcontrib><creatorcontrib>Souza, Glória E.P.</creatorcontrib><title>Endothelin-1 as a central mediator of LPS-induced fever in rats</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Fever induced by
E. coli lipopolysaccharide (LPS) in rats is substantially reduced by blockade of central endothelin ET
B receptors. This study explores the role of endothelin-1 as a central mediator of fever in rats, by investigating the effect of a pyrogenic dose of LPS on the levels of big endothelin-1 and endothelin-1 in the cerebrospinal fluid (CSF) and endothelin-1 in the plasma. We further assessed whether the increase in body temperature caused by central injection of endothelin-1 constitutes solely a hyperthermia or a true integrated febrile response. LPS (5 μg kg
−1, i.v.) induced fever which peaked at 1.16 ± 0.24 °C within 2 h and remained stable up to 5 h. CSF levels of immunoreactive (ir) big endothelin-1 decreased to undetectable levels at 3 h after LPS, returning only partially at 5 h post-injection. CSF ir-endothelin-1 levels were undetectable in saline-treated animals, but reached 21.9 ± 5.2 fmol ml
−1 at 3 h after LPS treatment. Plasma ir-endothelin-1 levels were unchanged after saline or LPS. Central injection of endothelin-1 (1 pmol, i.c.v.) caused long-lasting increases in body temperature (0.81 ± 0.17 °C, 3 h), but simultaneously decreased tail skin temperature (−1.10 ± 0.26 °C), indicating cutaneous vasoconstriction. Moreover, endothelin-1 induced fever (1.0 ± 0.3 °C, 3 h) when injected into the preoptic area of the anterior hypothalamus (100 fmol), but not i.v. (1 or 10 pmol). These data suggest that endothelin-1 is produced in the brain and acts centrally as a mediator of LPS-induced fever.</description><subject>Animals</subject><subject>Antihypertensive Agents - pharmacology</subject><subject>Big endothelin-1</subject><subject>Biological and medical sciences</subject><subject>Body Temperature - drug effects</subject><subject>Cerebrospinal fluid</subject><subject>Endothelin-1</subject><subject>Endothelin-1 - cerebrospinal fluid</subject><subject>Endothelin-1 - metabolism</subject><subject>Endothelin-1 - pharmacology</subject><subject>Endothelin-1 - physiology</subject><subject>Escherichia coli</subject><subject>Fever</subject><subject>Fever - chemically induced</subject><subject>Fever - physiopathology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypothalamus</subject><subject>Injections, Intravenous</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharides</subject><subject>Male</subject><subject>Microinjections</subject><subject>Oligopeptides - pharmacology</subject><subject>Piperidines - pharmacology</subject><subject>Preoptic area</subject><subject>Preoptic Area - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Skin Temperature - drug effects</subject><subject>Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3DAQgEVpaTaPvxB0SW_ejB6W7VNaQl6w0EKTsxhLY6rFK6eSN5B_Hy27Icechhm-eX2MnQtYChDmcr3sE4aYKC8lQF2KS1DNF7YQbSMrIzV8ZQsAMFXbdeqIHee8LqlSHXxnR8IoA6buFuzqJvpp_kdjiJXgmDlyR3FOOPIN-YDzlPg08NWfv1WIfuvI84FeKPEQecI5n7JvA46Zzg7xhD3d3jxe31er33cP179WldOdmitZCy1l3zjlJTZeeu2MA2MkUu89AnjtpdFD37aN0D3C4JWqe2qlphoUqhP2Yz_3OU3_t5RnuwnZ0ThipGmbrWnLn42sPwVFow1I2RbQ7EGXppwTDfY5hQ2mVyvA7hzbtX13bHeOd_XiuDSeHzZs--Loo-0gtQAXBwCzw3FIGF3IH1yjainU7tSfe46KuJdAyWYXKBbHIZGbrZ_CZ7e8AaHVnAY</recordid><startdate>20051220</startdate><enddate>20051220</enddate><creator>Fabricio, Aline S.C.</creator><creator>Rae, Giles A.</creator><creator>D'Orléans-Juste, Pedro</creator><creator>Souza, Glória E.P.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20051220</creationdate><title>Endothelin-1 as a central mediator of LPS-induced fever in rats</title><author>Fabricio, Aline S.C. ; Rae, Giles A. ; D'Orléans-Juste, Pedro ; Souza, Glória E.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-251422b7c3d2a7d2d4c6c0662aebdda00d4d264fb88714ba0fd335be824e503a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antihypertensive Agents - pharmacology</topic><topic>Big endothelin-1</topic><topic>Biological and medical sciences</topic><topic>Body Temperature - drug effects</topic><topic>Cerebrospinal fluid</topic><topic>Endothelin-1</topic><topic>Endothelin-1 - cerebrospinal fluid</topic><topic>Endothelin-1 - metabolism</topic><topic>Endothelin-1 - pharmacology</topic><topic>Endothelin-1 - physiology</topic><topic>Escherichia coli</topic><topic>Fever</topic><topic>Fever - chemically induced</topic><topic>Fever - physiopathology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypothalamus</topic><topic>Injections, Intravenous</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharides</topic><topic>Male</topic><topic>Microinjections</topic><topic>Oligopeptides - pharmacology</topic><topic>Piperidines - pharmacology</topic><topic>Preoptic area</topic><topic>Preoptic Area - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Skin Temperature - drug effects</topic><topic>Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fabricio, Aline S.C.</creatorcontrib><creatorcontrib>Rae, Giles A.</creatorcontrib><creatorcontrib>D'Orléans-Juste, Pedro</creatorcontrib><creatorcontrib>Souza, Glória E.P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fabricio, Aline S.C.</au><au>Rae, Giles A.</au><au>D'Orléans-Juste, Pedro</au><au>Souza, Glória E.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelin-1 as a central mediator of LPS-induced fever in rats</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2005-12-20</date><risdate>2005</risdate><volume>1066</volume><issue>1</issue><spage>92</spage><epage>100</epage><pages>92-100</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Fever induced by
E. coli lipopolysaccharide (LPS) in rats is substantially reduced by blockade of central endothelin ET
B receptors. This study explores the role of endothelin-1 as a central mediator of fever in rats, by investigating the effect of a pyrogenic dose of LPS on the levels of big endothelin-1 and endothelin-1 in the cerebrospinal fluid (CSF) and endothelin-1 in the plasma. We further assessed whether the increase in body temperature caused by central injection of endothelin-1 constitutes solely a hyperthermia or a true integrated febrile response. LPS (5 μg kg
−1, i.v.) induced fever which peaked at 1.16 ± 0.24 °C within 2 h and remained stable up to 5 h. CSF levels of immunoreactive (ir) big endothelin-1 decreased to undetectable levels at 3 h after LPS, returning only partially at 5 h post-injection. CSF ir-endothelin-1 levels were undetectable in saline-treated animals, but reached 21.9 ± 5.2 fmol ml
−1 at 3 h after LPS treatment. Plasma ir-endothelin-1 levels were unchanged after saline or LPS. Central injection of endothelin-1 (1 pmol, i.c.v.) caused long-lasting increases in body temperature (0.81 ± 0.17 °C, 3 h), but simultaneously decreased tail skin temperature (−1.10 ± 0.26 °C), indicating cutaneous vasoconstriction. Moreover, endothelin-1 induced fever (1.0 ± 0.3 °C, 3 h) when injected into the preoptic area of the anterior hypothalamus (100 fmol), but not i.v. (1 or 10 pmol). These data suggest that endothelin-1 is produced in the brain and acts centrally as a mediator of LPS-induced fever.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>16360659</pmid><doi>10.1016/j.brainres.2005.10.037</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Antihypertensive Agents - pharmacology Big endothelin-1 Biological and medical sciences Body Temperature - drug effects Cerebrospinal fluid Endothelin-1 Endothelin-1 - cerebrospinal fluid Endothelin-1 - metabolism Endothelin-1 - pharmacology Endothelin-1 - physiology Escherichia coli Fever Fever - chemically induced Fever - physiopathology Fundamental and applied biological sciences. Psychology Hypothalamus Injections, Intravenous Lipopolysaccharide Lipopolysaccharides Male Microinjections Oligopeptides - pharmacology Piperidines - pharmacology Preoptic area Preoptic Area - physiology Rats Rats, Wistar Skin Temperature - drug effects Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Endothelin-1 as a central mediator of LPS-induced fever in rats |
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