Phytochemical and genetic analysis in selected chemotypes of Withania somnifera
Presence of active constituents (withanolides and withaferin A) from roots and leaves of Withania somnifera were analyzed by HPLC. The isolated compounds were elucidated on the basis of IR, NMR and mass spectral data. Genetic analysis was carried out employing AFLP. Positive correlation of main comp...
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| Veröffentlicht in: | Phytochemistry (Oxford) 2006-10, Vol.67 (20), p.2269-2276 |
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| container_title | Phytochemistry (Oxford) |
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| creator | Dhar, Rekha S. Verma, Vijeshwar Suri, Krishan A. Sangwan, Rajinder S. Satti, Naresh K. Kumar, Arun Tuli, Rakesh Qazi, Gulam N. |
| description | Presence of active constituents (withanolides and withaferin A) from roots and leaves of
Withania somnifera were analyzed by HPLC. The isolated compounds were elucidated on the basis of IR, NMR and mass spectral data. Genetic analysis was carried out employing AFLP. Positive correlation of main components was obtained with the DNA markers employing Jaccard’s similarity coefficient. The present investigation throws a fresh perspective of using AFLP markers to designate specific chemotypes for breeding and therapeutic studies.
The main active components and genetic profile of 15 selected accessions of
Withania somnifera Dunal. were analysed. Ethanolic extract of the dried roots/leaves of the plant was concentrated under pressure at 50
±
5
°C and was analysed for main compounds (withanolides and withaferin A) by HPLC. All the main components were found to be present in accessions (AGB 002, AGB 009, RSS 009, RSS 033). Correlation of these main components with their genetic factors, was undertaken using AFLP (amplified fragment length polymorphism) markers. Among 64 primers 7 yielded optimum polymorphism. A total of 913 polymorphic peaks were generated using these primers. Jaccard’s similarity coefficient indicated that accessions having almost the same active compounds clustered together. The present study demonstrates that AFLP can be successfully used to resolve the correlation of AFLP data with the presence of secondary metabolites. |
| doi_str_mv | 10.1016/j.phytochem.2006.07.014 |
| format | Article |
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Withania somnifera were analyzed by HPLC. The isolated compounds were elucidated on the basis of IR, NMR and mass spectral data. Genetic analysis was carried out employing AFLP. Positive correlation of main components was obtained with the DNA markers employing Jaccard’s similarity coefficient. The present investigation throws a fresh perspective of using AFLP markers to designate specific chemotypes for breeding and therapeutic studies.
The main active components and genetic profile of 15 selected accessions of
Withania somnifera Dunal. were analysed. Ethanolic extract of the dried roots/leaves of the plant was concentrated under pressure at 50
±
5
°C and was analysed for main compounds (withanolides and withaferin A) by HPLC. All the main components were found to be present in accessions (AGB 002, AGB 009, RSS 009, RSS 033). Correlation of these main components with their genetic factors, was undertaken using AFLP (amplified fragment length polymorphism) markers. Among 64 primers 7 yielded optimum polymorphism. A total of 913 polymorphic peaks were generated using these primers. Jaccard’s similarity coefficient indicated that accessions having almost the same active compounds clustered together. The present study demonstrates that AFLP can be successfully used to resolve the correlation of AFLP data with the presence of secondary metabolites.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/j.phytochem.2006.07.014</identifier><identifier>PMID: 16956635</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>AFLP ; amplified fragment length polymorphism ; Biological and medical sciences ; chemical analysis ; chemotaxonomy ; chemotypes ; Chromatography, High Pressure Liquid ; DNA Fingerprinting ; ergosterol ; Fundamental and applied biological sciences. Psychology ; Genetic Markers ; high performance liquid chromatography ; HPLC ; medicinal plants ; NMR ; phytochemicals ; Plant cytology, morphology, systematics, chorology and evolution ; Plant Extracts - chemistry ; Plant Leaves - chemistry ; Plant Leaves - genetics ; Plant Roots - chemistry ; Plant Roots - genetics ; plant taxonomy ; Polymorphism, Genetic ; secondary metabolites ; Solanaceae ; spectral analysis ; Spermatophyta ; Systematics (diagnosis, chromosome numbers) ; TLC ; withaferin A ; Withania - chemistry ; Withania - genetics ; Withania somnifera ; withanolides</subject><ispartof>Phytochemistry (Oxford), 2006-10, Vol.67 (20), p.2269-2276</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-1afcd508fb96b87f3f002e426ce5fa479fd959ce9fb35e40ab328115b94563873</citedby><cites>FETCH-LOGICAL-c454t-1afcd508fb96b87f3f002e426ce5fa479fd959ce9fb35e40ab328115b94563873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0031942206004183$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18139865$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16956635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dhar, Rekha S.</creatorcontrib><creatorcontrib>Verma, Vijeshwar</creatorcontrib><creatorcontrib>Suri, Krishan A.</creatorcontrib><creatorcontrib>Sangwan, Rajinder S.</creatorcontrib><creatorcontrib>Satti, Naresh K.</creatorcontrib><creatorcontrib>Kumar, Arun</creatorcontrib><creatorcontrib>Tuli, Rakesh</creatorcontrib><creatorcontrib>Qazi, Gulam N.</creatorcontrib><title>Phytochemical and genetic analysis in selected chemotypes of Withania somnifera</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>Presence of active constituents (withanolides and withaferin A) from roots and leaves of
Withania somnifera were analyzed by HPLC. The isolated compounds were elucidated on the basis of IR, NMR and mass spectral data. Genetic analysis was carried out employing AFLP. Positive correlation of main components was obtained with the DNA markers employing Jaccard’s similarity coefficient. The present investigation throws a fresh perspective of using AFLP markers to designate specific chemotypes for breeding and therapeutic studies.
The main active components and genetic profile of 15 selected accessions of
Withania somnifera Dunal. were analysed. Ethanolic extract of the dried roots/leaves of the plant was concentrated under pressure at 50
±
5
°C and was analysed for main compounds (withanolides and withaferin A) by HPLC. All the main components were found to be present in accessions (AGB 002, AGB 009, RSS 009, RSS 033). Correlation of these main components with their genetic factors, was undertaken using AFLP (amplified fragment length polymorphism) markers. Among 64 primers 7 yielded optimum polymorphism. A total of 913 polymorphic peaks were generated using these primers. Jaccard’s similarity coefficient indicated that accessions having almost the same active compounds clustered together. The present study demonstrates that AFLP can be successfully used to resolve the correlation of AFLP data with the presence of secondary metabolites.</description><subject>AFLP</subject><subject>amplified fragment length polymorphism</subject><subject>Biological and medical sciences</subject><subject>chemical analysis</subject><subject>chemotaxonomy</subject><subject>chemotypes</subject><subject>Chromatography, High Pressure Liquid</subject><subject>DNA Fingerprinting</subject><subject>ergosterol</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Markers</subject><subject>high performance liquid chromatography</subject><subject>HPLC</subject><subject>medicinal plants</subject><subject>NMR</subject><subject>phytochemicals</subject><subject>Plant cytology, morphology, systematics, chorology and evolution</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - genetics</subject><subject>Plant Roots - chemistry</subject><subject>Plant Roots - genetics</subject><subject>plant taxonomy</subject><subject>Polymorphism, Genetic</subject><subject>secondary metabolites</subject><subject>Solanaceae</subject><subject>spectral analysis</subject><subject>Spermatophyta</subject><subject>Systematics (diagnosis, chromosome numbers)</subject><subject>TLC</subject><subject>withaferin A</subject><subject>Withania - chemistry</subject><subject>Withania - genetics</subject><subject>Withania somnifera</subject><subject>withanolides</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQQK2qVVmgfwFyaW9Jx3Hs2EeE2lIJiUot4mg5zpj1KokXO4u0_x5Hu4Ujp5nDm683hFxSqChQ8X1Tbdf7Odg1jlUNICpoK6DNB7KismUlawE-khUAo6Vq6vqEnKa0AQDOhfhMTqhQOWF8Re7-_O_jrRkKM_XFI044e5tzM-yTT4WfioQD2hn7YiHDvN9iKoIrHvy8NpM3RQrj5B1Gc04-OTMk_HKMZ-T-549_1zfl7d2v39dXt6VteDOX1Djbc5CuU6KTrWMOoMamFha5M02rXK-4sqhcxzg2YDpWS0p5pxouWD7xjHw79N3G8LTDNOvRJ4vDYCYMu6SFlEpkDe-CVDFGpWAZbA-gjSGliE5vox9N3GsKepGuN_pVul6ka2h1lp4rL44jdt2I_Vvd0XIGvh4Bk7JlF81kfXrjJGVKioW7PHDOBG0eY2bu_9ZAGVAKUsJyzdWBwOz22WPUyXqcLPY-5gfpPvh3130BZRyuFQ</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Dhar, Rekha S.</creator><creator>Verma, Vijeshwar</creator><creator>Suri, Krishan A.</creator><creator>Sangwan, Rajinder S.</creator><creator>Satti, Naresh K.</creator><creator>Kumar, Arun</creator><creator>Tuli, Rakesh</creator><creator>Qazi, Gulam N.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>Phytochemical and genetic analysis in selected chemotypes of Withania somnifera</title><author>Dhar, Rekha S. ; Verma, Vijeshwar ; Suri, Krishan A. ; Sangwan, Rajinder S. ; Satti, Naresh K. ; Kumar, Arun ; Tuli, Rakesh ; Qazi, Gulam N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-1afcd508fb96b87f3f002e426ce5fa479fd959ce9fb35e40ab328115b94563873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>AFLP</topic><topic>amplified fragment length polymorphism</topic><topic>Biological and medical sciences</topic><topic>chemical analysis</topic><topic>chemotaxonomy</topic><topic>chemotypes</topic><topic>Chromatography, High Pressure Liquid</topic><topic>DNA Fingerprinting</topic><topic>ergosterol</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Markers</topic><topic>high performance liquid chromatography</topic><topic>HPLC</topic><topic>medicinal plants</topic><topic>NMR</topic><topic>phytochemicals</topic><topic>Plant cytology, morphology, systematics, chorology and evolution</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - genetics</topic><topic>Plant Roots - chemistry</topic><topic>Plant Roots - genetics</topic><topic>plant taxonomy</topic><topic>Polymorphism, Genetic</topic><topic>secondary metabolites</topic><topic>Solanaceae</topic><topic>spectral analysis</topic><topic>Spermatophyta</topic><topic>Systematics (diagnosis, chromosome numbers)</topic><topic>TLC</topic><topic>withaferin A</topic><topic>Withania - chemistry</topic><topic>Withania - genetics</topic><topic>Withania somnifera</topic><topic>withanolides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dhar, Rekha S.</creatorcontrib><creatorcontrib>Verma, Vijeshwar</creatorcontrib><creatorcontrib>Suri, Krishan A.</creatorcontrib><creatorcontrib>Sangwan, Rajinder S.</creatorcontrib><creatorcontrib>Satti, Naresh K.</creatorcontrib><creatorcontrib>Kumar, Arun</creatorcontrib><creatorcontrib>Tuli, Rakesh</creatorcontrib><creatorcontrib>Qazi, Gulam N.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dhar, Rekha S.</au><au>Verma, Vijeshwar</au><au>Suri, Krishan A.</au><au>Sangwan, Rajinder S.</au><au>Satti, Naresh K.</au><au>Kumar, Arun</au><au>Tuli, Rakesh</au><au>Qazi, Gulam N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytochemical and genetic analysis in selected chemotypes of Withania somnifera</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>67</volume><issue>20</issue><spage>2269</spage><epage>2276</epage><pages>2269-2276</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>Presence of active constituents (withanolides and withaferin A) from roots and leaves of
Withania somnifera were analyzed by HPLC. The isolated compounds were elucidated on the basis of IR, NMR and mass spectral data. Genetic analysis was carried out employing AFLP. Positive correlation of main components was obtained with the DNA markers employing Jaccard’s similarity coefficient. The present investigation throws a fresh perspective of using AFLP markers to designate specific chemotypes for breeding and therapeutic studies.
The main active components and genetic profile of 15 selected accessions of
Withania somnifera Dunal. were analysed. Ethanolic extract of the dried roots/leaves of the plant was concentrated under pressure at 50
±
5
°C and was analysed for main compounds (withanolides and withaferin A) by HPLC. All the main components were found to be present in accessions (AGB 002, AGB 009, RSS 009, RSS 033). Correlation of these main components with their genetic factors, was undertaken using AFLP (amplified fragment length polymorphism) markers. Among 64 primers 7 yielded optimum polymorphism. A total of 913 polymorphic peaks were generated using these primers. Jaccard’s similarity coefficient indicated that accessions having almost the same active compounds clustered together. The present study demonstrates that AFLP can be successfully used to resolve the correlation of AFLP data with the presence of secondary metabolites.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>16956635</pmid><doi>10.1016/j.phytochem.2006.07.014</doi><tpages>8</tpages></addata></record> |
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| subjects | AFLP amplified fragment length polymorphism Biological and medical sciences chemical analysis chemotaxonomy chemotypes Chromatography, High Pressure Liquid DNA Fingerprinting ergosterol Fundamental and applied biological sciences. Psychology Genetic Markers high performance liquid chromatography HPLC medicinal plants NMR phytochemicals Plant cytology, morphology, systematics, chorology and evolution Plant Extracts - chemistry Plant Leaves - chemistry Plant Leaves - genetics Plant Roots - chemistry Plant Roots - genetics plant taxonomy Polymorphism, Genetic secondary metabolites Solanaceae spectral analysis Spermatophyta Systematics (diagnosis, chromosome numbers) TLC withaferin A Withania - chemistry Withania - genetics Withania somnifera withanolides |
| title | Phytochemical and genetic analysis in selected chemotypes of Withania somnifera |
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