Gastric estrogen directly induces ghrelin expression and production in the rat stomach
Ghrelin, an endogenous ligand for the GH secretagogue receptor, is predominantly produced in the stomach. Little is known about the regulation mechanism of gastric ghrelin. Here, we report that estrogen synthesized in the stomach induces rat gastric ghrelin gene expression and production. We establi...
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| Veröffentlicht in: | Journal of endocrinology 2006-09, Vol.190 (3), p.749-757 |
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| creator | Sakata, Ichiro Tanaka, Toru Yamazaki, Mami Tanizaki, Takashi Zheng, Zhao Sakai, Takafumi |
| description | Ghrelin, an endogenous ligand for the GH secretagogue receptor, is predominantly produced in the stomach. Little is known about the regulation mechanism of gastric ghrelin. Here, we report that estrogen synthesized in the stomach induces rat gastric ghrelin gene expression and production. We established a gastric ghrelin cell enrichment method using Percoll centrifugation and then studied the effect of estrogen and/or its antagonist on ghrelin expression and production. Treatment with estrogen for 8 h significantly increased the level of ghrelin expression, and ICI-182 780, an estrogen receptor (ER) antagonist, completely reversed this effect. Reverse transcriptase-PCR analysis clearly showed that ERα and aromatase are expressed in the female rat stomach. Moreover, treatment with an aromatase inhibitor, 4-hydro-xyandrostenedione (formestane), significantly decreased the level of ghrelin mRNA expression in minced stomach tissue. In vivo studies revealed that the ghrelin mRNA expression and production did not change in gonadectomized rat 3 weeks after surgery. These results strongly suggest that estrogen produced in the stomach directly induces ghrelin expression and production in both female and male rat stomachs. |
| doi_str_mv | 10.1677/joe.1.06808 |
| format | Article |
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Little is known about the regulation mechanism of gastric ghrelin. Here, we report that estrogen synthesized in the stomach induces rat gastric ghrelin gene expression and production. We established a gastric ghrelin cell enrichment method using Percoll centrifugation and then studied the effect of estrogen and/or its antagonist on ghrelin expression and production. Treatment with estrogen for 8 h significantly increased the level of ghrelin expression, and ICI-182 780, an estrogen receptor (ER) antagonist, completely reversed this effect. Reverse transcriptase-PCR analysis clearly showed that ERα and aromatase are expressed in the female rat stomach. Moreover, treatment with an aromatase inhibitor, 4-hydro-xyandrostenedione (formestane), significantly decreased the level of ghrelin mRNA expression in minced stomach tissue. In vivo studies revealed that the ghrelin mRNA expression and production did not change in gonadectomized rat 3 weeks after surgery. These results strongly suggest that estrogen produced in the stomach directly induces ghrelin expression and production in both female and male rat stomachs.</description><identifier>ISSN: 0022-0795</identifier><identifier>EISSN: 1479-6805</identifier><identifier>DOI: 10.1677/joe.1.06808</identifier><identifier>PMID: 17003276</identifier><identifier>CODEN: JOENAK</identifier><language>eng</language><publisher>Colchester: BioScientifica</publisher><subject>Androstenedione - analogs & derivatives ; Androstenedione - pharmacology ; Animals ; Aromatase - genetics ; Aromatase Inhibitors - pharmacology ; Biological and medical sciences ; Cells, Cultured ; Estradiol - pharmacology ; Estrogen Receptor alpha - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Gastric Mucosa - chemistry ; Ghrelin ; Immunohistochemistry - methods ; Male ; Orchiectomy ; Ovariectomy ; Peptide Hormones - analysis ; Peptide Hormones - genetics ; Peptide Hormones - metabolism ; Radioimmunoassay - methods ; Rats ; Rats, Wistar ; Regular papers ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Stomach ; Stomach - drug effects ; Stomach - metabolism ; Vertebrates: digestive system</subject><ispartof>Journal of endocrinology, 2006-09, Vol.190 (3), p.749-757</ispartof><rights>Society for Endocrinology</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b505t-e6f12b76c1405da945141fe6be22eace6860c3b4ea4a7a901796cae9d1ae1efb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18145498$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17003276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakata, Ichiro</creatorcontrib><creatorcontrib>Tanaka, Toru</creatorcontrib><creatorcontrib>Yamazaki, Mami</creatorcontrib><creatorcontrib>Tanizaki, Takashi</creatorcontrib><creatorcontrib>Zheng, Zhao</creatorcontrib><creatorcontrib>Sakai, Takafumi</creatorcontrib><title>Gastric estrogen directly induces ghrelin expression and production in the rat stomach</title><title>Journal of endocrinology</title><addtitle>J Endocrinol</addtitle><description>Ghrelin, an endogenous ligand for the GH secretagogue receptor, is predominantly produced in the stomach. Little is known about the regulation mechanism of gastric ghrelin. Here, we report that estrogen synthesized in the stomach induces rat gastric ghrelin gene expression and production. We established a gastric ghrelin cell enrichment method using Percoll centrifugation and then studied the effect of estrogen and/or its antagonist on ghrelin expression and production. Treatment with estrogen for 8 h significantly increased the level of ghrelin expression, and ICI-182 780, an estrogen receptor (ER) antagonist, completely reversed this effect. Reverse transcriptase-PCR analysis clearly showed that ERα and aromatase are expressed in the female rat stomach. Moreover, treatment with an aromatase inhibitor, 4-hydro-xyandrostenedione (formestane), significantly decreased the level of ghrelin mRNA expression in minced stomach tissue. In vivo studies revealed that the ghrelin mRNA expression and production did not change in gonadectomized rat 3 weeks after surgery. These results strongly suggest that estrogen produced in the stomach directly induces ghrelin expression and production in both female and male rat stomachs.</description><subject>Androstenedione - analogs & derivatives</subject><subject>Androstenedione - pharmacology</subject><subject>Animals</subject><subject>Aromatase - genetics</subject><subject>Aromatase Inhibitors - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen Receptor alpha - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastric Mucosa - chemistry</subject><subject>Ghrelin</subject><subject>Immunohistochemistry - methods</subject><subject>Male</subject><subject>Orchiectomy</subject><subject>Ovariectomy</subject><subject>Peptide Hormones - analysis</subject><subject>Peptide Hormones - genetics</subject><subject>Peptide Hormones - metabolism</subject><subject>Radioimmunoassay - methods</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Regular papers</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Stomach</subject><subject>Stomach - drug effects</subject><subject>Stomach - metabolism</subject><subject>Vertebrates: digestive system</subject><issn>0022-0795</issn><issn>1479-6805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rGzEQhkVpqB23p96LLu0lrKNZ62P3GELzAYZckl7FrHbWq7DedaQ1Sf595NhgKCSnkZhH74wexn6CmIM25vxxoDnMhS5E8YVNQZoyS2f1lU2FyPNMmFJN2GmMj0KAArP4xiZghFjkRk_Zv2uMY_COUyrDinpe-0Bu7F657-uto8hXbaDO95xeNoFi9EPPsa_5JgypP-6uqTm2xAOOPI7DGl37nZ002EX6cagz9nD19_7yJlveXd9eXiyzSgk1ZqQbyCujHUihaiylAgkN6YrynNCRLrRwi0oSSjRYCjCldkhlDUhATbWYsT_73LTN0zb9wa59dNR12NOwjVYXRSm1KhJ4tgddGGIM1NhN8GsMrxaE3Wm0SaMF-64x0b8OsdtqTfWRPXhLwO8DgNFh1wTsnY9HrgCpZLkLyvdc61ftczJrKz9E56kffeMdfjAd9o_-Yz_b-A2qh55N</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Sakata, Ichiro</creator><creator>Tanaka, Toru</creator><creator>Yamazaki, Mami</creator><creator>Tanizaki, Takashi</creator><creator>Zheng, Zhao</creator><creator>Sakai, Takafumi</creator><general>BioScientifica</general><general>Portland Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>Gastric estrogen directly induces ghrelin expression and production in the rat stomach</title><author>Sakata, Ichiro ; Tanaka, Toru ; Yamazaki, Mami ; Tanizaki, Takashi ; Zheng, Zhao ; Sakai, Takafumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b505t-e6f12b76c1405da945141fe6be22eace6860c3b4ea4a7a901796cae9d1ae1efb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Androstenedione - analogs & derivatives</topic><topic>Androstenedione - pharmacology</topic><topic>Animals</topic><topic>Aromatase - genetics</topic><topic>Aromatase Inhibitors - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen Receptor alpha - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastric Mucosa - chemistry</topic><topic>Ghrelin</topic><topic>Immunohistochemistry - methods</topic><topic>Male</topic><topic>Orchiectomy</topic><topic>Ovariectomy</topic><topic>Peptide Hormones - analysis</topic><topic>Peptide Hormones - genetics</topic><topic>Peptide Hormones - metabolism</topic><topic>Radioimmunoassay - methods</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Regular papers</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Stomach</topic><topic>Stomach - drug effects</topic><topic>Stomach - metabolism</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakata, Ichiro</creatorcontrib><creatorcontrib>Tanaka, Toru</creatorcontrib><creatorcontrib>Yamazaki, Mami</creatorcontrib><creatorcontrib>Tanizaki, Takashi</creatorcontrib><creatorcontrib>Zheng, Zhao</creatorcontrib><creatorcontrib>Sakai, Takafumi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakata, Ichiro</au><au>Tanaka, Toru</au><au>Yamazaki, Mami</au><au>Tanizaki, Takashi</au><au>Zheng, Zhao</au><au>Sakai, Takafumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gastric estrogen directly induces ghrelin expression and production in the rat stomach</atitle><jtitle>Journal of endocrinology</jtitle><addtitle>J Endocrinol</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>190</volume><issue>3</issue><spage>749</spage><epage>757</epage><pages>749-757</pages><issn>0022-0795</issn><eissn>1479-6805</eissn><coden>JOENAK</coden><abstract>Ghrelin, an endogenous ligand for the GH secretagogue receptor, is predominantly produced in the stomach. Little is known about the regulation mechanism of gastric ghrelin. Here, we report that estrogen synthesized in the stomach induces rat gastric ghrelin gene expression and production. We established a gastric ghrelin cell enrichment method using Percoll centrifugation and then studied the effect of estrogen and/or its antagonist on ghrelin expression and production. Treatment with estrogen for 8 h significantly increased the level of ghrelin expression, and ICI-182 780, an estrogen receptor (ER) antagonist, completely reversed this effect. Reverse transcriptase-PCR analysis clearly showed that ERα and aromatase are expressed in the female rat stomach. Moreover, treatment with an aromatase inhibitor, 4-hydro-xyandrostenedione (formestane), significantly decreased the level of ghrelin mRNA expression in minced stomach tissue. In vivo studies revealed that the ghrelin mRNA expression and production did not change in gonadectomized rat 3 weeks after surgery. These results strongly suggest that estrogen produced in the stomach directly induces ghrelin expression and production in both female and male rat stomachs.</abstract><cop>Colchester</cop><pub>BioScientifica</pub><pmid>17003276</pmid><doi>10.1677/joe.1.06808</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Androstenedione - analogs & derivatives Androstenedione - pharmacology Animals Aromatase - genetics Aromatase Inhibitors - pharmacology Biological and medical sciences Cells, Cultured Estradiol - pharmacology Estrogen Receptor alpha - genetics Female Fundamental and applied biological sciences. Psychology Gastric Mucosa - chemistry Ghrelin Immunohistochemistry - methods Male Orchiectomy Ovariectomy Peptide Hormones - analysis Peptide Hormones - genetics Peptide Hormones - metabolism Radioimmunoassay - methods Rats Rats, Wistar Regular papers Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Stomach Stomach - drug effects Stomach - metabolism Vertebrates: digestive system |
| title | Gastric estrogen directly induces ghrelin expression and production in the rat stomach |
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