Sex differences in connexin-43 expression in left ventricles of aging rats
Cardiac gap junctions have been implicated in maintaining intercellular electrical and metabolic couplings. The abnormalities in connexin-43 (Cx43) lead to conduction defects and contractile dysfunction. We have evaluated the expression and phoshorylation status of Cx43 in the left ventricular myoca...
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| Veröffentlicht in: | Physiological research 2005-01, Vol.54 (6), p.705-708 |
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| creator | Tribulová, N Dupont, E Soukup, T Okruhlicová, L Severs, N J |
| description | Cardiac gap junctions have been implicated in maintaining intercellular electrical and metabolic couplings. The abnormalities in connexin-43 (Cx43) lead to conduction defects and contractile dysfunction. We have evaluated the expression and phoshorylation status of Cx43 in the left ventricular myocardium of male and female 16-month-old rats submitted to 14-week L-thyroxine (T4) treatment. Western blot analysis revealed the presence of fully or intermediately phosphorylated and unphosphorylated forms of Cx43. We have found no significant differences in Cx43 expression and phosphorylation between T4-treated and control untreated animals. However, expression of Cx43 was significantly higher in female compared to male rats. We conclude that T4 administration has no effect on Cx43 expression, but there are sex-dependent differences in Cx43 expression in the left ventricles between aging male and female rats. |
| doi_str_mv | 10.33549/physiolres.930000.54.705 |
| format | Article |
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The abnormalities in connexin-43 (Cx43) lead to conduction defects and contractile dysfunction. We have evaluated the expression and phoshorylation status of Cx43 in the left ventricular myocardium of male and female 16-month-old rats submitted to 14-week L-thyroxine (T4) treatment. Western blot analysis revealed the presence of fully or intermediately phosphorylated and unphosphorylated forms of Cx43. We have found no significant differences in Cx43 expression and phosphorylation between T4-treated and control untreated animals. However, expression of Cx43 was significantly higher in female compared to male rats. We conclude that T4 administration has no effect on Cx43 expression, but there are sex-dependent differences in Cx43 expression in the left ventricles between aging male and female rats.</description><identifier>ISSN: 0862-8408</identifier><identifier>EISSN: 1802-9973</identifier><identifier>DOI: 10.33549/physiolres.930000.54.705</identifier><identifier>PMID: 16351499</identifier><language>eng</language><publisher>Czech Republic: Institute of Physiology</publisher><subject>Aging - metabolism ; Animals ; Connexin 43 - biosynthesis ; Connexin 43 - metabolism ; Female ; Heart Ventricles - metabolism ; Male ; Myocardium - metabolism ; Phosphorylation ; Rats ; Rats, Wistar ; Sex Factors</subject><ispartof>Physiological research, 2005-01, Vol.54 (6), p.705-708</ispartof><rights>Copyright Institute of Physiology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16351499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tribulová, N</creatorcontrib><creatorcontrib>Dupont, E</creatorcontrib><creatorcontrib>Soukup, T</creatorcontrib><creatorcontrib>Okruhlicová, L</creatorcontrib><creatorcontrib>Severs, N J</creatorcontrib><title>Sex differences in connexin-43 expression in left ventricles of aging rats</title><title>Physiological research</title><addtitle>Physiol Res</addtitle><description>Cardiac gap junctions have been implicated in maintaining intercellular electrical and metabolic couplings. The abnormalities in connexin-43 (Cx43) lead to conduction defects and contractile dysfunction. We have evaluated the expression and phoshorylation status of Cx43 in the left ventricular myocardium of male and female 16-month-old rats submitted to 14-week L-thyroxine (T4) treatment. Western blot analysis revealed the presence of fully or intermediately phosphorylated and unphosphorylated forms of Cx43. We have found no significant differences in Cx43 expression and phosphorylation between T4-treated and control untreated animals. However, expression of Cx43 was significantly higher in female compared to male rats. We conclude that T4 administration has no effect on Cx43 expression, but there are sex-dependent differences in Cx43 expression in the left ventricles between aging male and female rats.</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Connexin 43 - biosynthesis</subject><subject>Connexin 43 - metabolism</subject><subject>Female</subject><subject>Heart Ventricles - metabolism</subject><subject>Male</subject><subject>Myocardium - metabolism</subject><subject>Phosphorylation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sex Factors</subject><issn>0862-8408</issn><issn>1802-9973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkD1PwzAQhi0EoqXwF1BgYEtwfHbsjKjiU5UYgDlyknNxlTrBTlH773FFWbjlhnt07wchVznNAAQvb4fPXbB95zFkJdA4meCZpOKITHNFWVqWEo7JlKqCpYpTNSFnIawoZZJKOCWTvACR87Kckpc33CatNQY9ugZDYl3S9M7h1rqUQ4LbIapEMbe_dGjG5Bvd6G3TRbg3iV5at0y8HsM5OTG6C3hx2DPy8XD_Pn9KF6-Pz_O7RTowKMa0LRQX0LS1kHWtWtFGgzVy4A2CZLmGFnitoTZGFkrwGA4KraXm-1BIDczIze_fwfdfGwxjtbahwa7TDvtNqAqlSiagiOD1P3DVb7yL3iqWs1woBipClwdoU6-xrQZv19rvqr-K4AdadGtC</recordid><startdate>20050101</startdate><enddate>20050101</enddate><creator>Tribulová, N</creator><creator>Dupont, E</creator><creator>Soukup, T</creator><creator>Okruhlicová, L</creator><creator>Severs, N J</creator><general>Institute of Physiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20050101</creationdate><title>Sex differences in connexin-43 expression in left ventricles of aging rats</title><author>Tribulová, N ; Dupont, E ; Soukup, T ; Okruhlicová, L ; Severs, N J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p236t-d68453cdb57bb8d5d840be434ce3721a3d34ba3bff7685400036aa7a49973e0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Connexin 43 - biosynthesis</topic><topic>Connexin 43 - metabolism</topic><topic>Female</topic><topic>Heart Ventricles - metabolism</topic><topic>Male</topic><topic>Myocardium - metabolism</topic><topic>Phosphorylation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sex Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tribulová, N</creatorcontrib><creatorcontrib>Dupont, E</creatorcontrib><creatorcontrib>Soukup, T</creatorcontrib><creatorcontrib>Okruhlicová, L</creatorcontrib><creatorcontrib>Severs, N J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Physiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tribulová, N</au><au>Dupont, E</au><au>Soukup, T</au><au>Okruhlicová, L</au><au>Severs, N J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sex differences in connexin-43 expression in left ventricles of aging rats</atitle><jtitle>Physiological research</jtitle><addtitle>Physiol Res</addtitle><date>2005-01-01</date><risdate>2005</risdate><volume>54</volume><issue>6</issue><spage>705</spage><epage>708</epage><pages>705-708</pages><issn>0862-8408</issn><eissn>1802-9973</eissn><abstract>Cardiac gap junctions have been implicated in maintaining intercellular electrical and metabolic couplings. The abnormalities in connexin-43 (Cx43) lead to conduction defects and contractile dysfunction. We have evaluated the expression and phoshorylation status of Cx43 in the left ventricular myocardium of male and female 16-month-old rats submitted to 14-week L-thyroxine (T4) treatment. Western blot analysis revealed the presence of fully or intermediately phosphorylated and unphosphorylated forms of Cx43. We have found no significant differences in Cx43 expression and phosphorylation between T4-treated and control untreated animals. However, expression of Cx43 was significantly higher in female compared to male rats. We conclude that T4 administration has no effect on Cx43 expression, but there are sex-dependent differences in Cx43 expression in the left ventricles between aging male and female rats.</abstract><cop>Czech Republic</cop><pub>Institute of Physiology</pub><pmid>16351499</pmid><doi>10.33549/physiolres.930000.54.705</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aging - metabolism Animals Connexin 43 - biosynthesis Connexin 43 - metabolism Female Heart Ventricles - metabolism Male Myocardium - metabolism Phosphorylation Rats Rats, Wistar Sex Factors |
| title | Sex differences in connexin-43 expression in left ventricles of aging rats |
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