Acetone extraction of mcl-PHA from Pseudomonas putida KT2440
A methodology was developed for the extraction of medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) from Pseudomonas putida. It was determined that if dry P. putida biomass containing mcl-PHA was washed in 20 volumes of methanol for 5 min followed by Soxhlet extraction in 10 volumes of acetone...
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| Veröffentlicht in: | Journal of microbiological methods 2006-11, Vol.67 (2), p.212-219 |
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| container_title | Journal of microbiological methods |
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| creator | Jiang, Xuan Ramsay, Juliana A. Ramsay, Bruce A. |
| description | A methodology was developed for the extraction of medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) from
Pseudomonas putida. It was determined that if dry
P. putida biomass containing mcl-PHA was washed in 20 volumes of methanol for 5 min followed by Soxhlet extraction in 10 volumes of acetone for 5 h, almost all of the PHA could be recovered with no detectable loss of molecular weight. Biomass containing higher amounts of PHA required less methanol during the pretreatment step but more acetone in the solvent extraction step than biomass containing less PHA. Further purification could be achieved by redissolving the PHA in acetone and reprecipitating in cold methanol. UV spectroscopy at 241 and 275 nm could be used as an indication of product purity. |
| doi_str_mv | 10.1016/j.mimet.2006.03.015 |
| format | Article |
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Pseudomonas putida. It was determined that if dry
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Pseudomonas putida. It was determined that if dry
P. putida biomass containing mcl-PHA was washed in 20 volumes of methanol for 5 min followed by Soxhlet extraction in 10 volumes of acetone for 5 h, almost all of the PHA could be recovered with no detectable loss of molecular weight. Biomass containing higher amounts of PHA required less methanol during the pretreatment step but more acetone in the solvent extraction step than biomass containing less PHA. Further purification could be achieved by redissolving the PHA in acetone and reprecipitating in cold methanol. UV spectroscopy at 241 and 275 nm could be used as an indication of product purity.</description><subject>Acetone</subject><subject>Acetone - chemistry</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Extraction</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Industrial Microbiology - methods</subject><subject>Medium-chain-length polyhydroxyalkanoates</subject><subject>Microbiology</subject><subject>PHA</subject><subject>Polyesters - isolation & purification</subject><subject>Polyhydroxyalkanoates</subject><subject>Pseudomonas putida</subject><subject>Pseudomonas putida - chemistry</subject><subject>Separation</subject><subject>Solvent</subject><subject>Solvents - chemistry</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0Mtq3DAUgGERUprptE8QCN6kO7u62ZIhWQyhbUoDzSJdi2PpCDTY1kSyS_r2tTsD2bXaCMSng_QTcsloxShrPu2rIQw4VZzSpqKioqw-IxumFS-1qNtzslmUKhVl_IK8y3lPFyGkfksulvNacFFvyM3O4hRHLPBlSmCnEMci-mKwffl4vyt8ikPxmHF2cYgj5OIwT8FB8f2JS0nfkzce-owfTvuW_Pzy-enuvnz48fXb3e6htFLLqbQAHeuahtq2Vdw5qqRovVJIdSdrz7WFTnAGEinomrlOdgqtd8DAgndMbMnH49xDis8z5skMIVvsexgxztk0el1c_Reytm0aUTcLFEdoU8w5oTeHFAZIvw2jZq1r9uZvXbPWNVSYtd2WXJ3Gz92A7vXOKecCrk8AsoXeJxhtyK9OM8UpXz90e3S4VPsVMJlsA44WXUhoJ-Ni-OdD_gA5fJgK</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Jiang, Xuan</creator><creator>Ramsay, Juliana A.</creator><creator>Ramsay, Bruce A.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20061101</creationdate><title>Acetone extraction of mcl-PHA from Pseudomonas putida KT2440</title><author>Jiang, Xuan ; Ramsay, Juliana A. ; Ramsay, Bruce A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-caab1b660c9972dd07439f77e08b45f28cab321a4e0a851db4b7ecfda1acafd13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acetone</topic><topic>Acetone - chemistry</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Extraction</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Industrial Microbiology - methods</topic><topic>Medium-chain-length polyhydroxyalkanoates</topic><topic>Microbiology</topic><topic>PHA</topic><topic>Polyesters - isolation & purification</topic><topic>Polyhydroxyalkanoates</topic><topic>Pseudomonas putida</topic><topic>Pseudomonas putida - chemistry</topic><topic>Separation</topic><topic>Solvent</topic><topic>Solvents - chemistry</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Xuan</creatorcontrib><creatorcontrib>Ramsay, Juliana A.</creatorcontrib><creatorcontrib>Ramsay, Bruce A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Xuan</au><au>Ramsay, Juliana A.</au><au>Ramsay, Bruce A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acetone extraction of mcl-PHA from Pseudomonas putida KT2440</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>67</volume><issue>2</issue><spage>212</spage><epage>219</epage><pages>212-219</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>A methodology was developed for the extraction of medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) from
Pseudomonas putida. It was determined that if dry
P. putida biomass containing mcl-PHA was washed in 20 volumes of methanol for 5 min followed by Soxhlet extraction in 10 volumes of acetone for 5 h, almost all of the PHA could be recovered with no detectable loss of molecular weight. Biomass containing higher amounts of PHA required less methanol during the pretreatment step but more acetone in the solvent extraction step than biomass containing less PHA. Further purification could be achieved by redissolving the PHA in acetone and reprecipitating in cold methanol. UV spectroscopy at 241 and 275 nm could be used as an indication of product purity.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>16753235</pmid><doi>10.1016/j.mimet.2006.03.015</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of microbiological methods, 2006-11, Vol.67 (2), p.212-219 |
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| subjects | Acetone Acetone - chemistry Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Biomass Extraction Fundamental and applied biological sciences. Psychology Industrial Microbiology - methods Medium-chain-length polyhydroxyalkanoates Microbiology PHA Polyesters - isolation & purification Polyhydroxyalkanoates Pseudomonas putida Pseudomonas putida - chemistry Separation Solvent Solvents - chemistry Spectrophotometry, Ultraviolet |
| title | Acetone extraction of mcl-PHA from Pseudomonas putida KT2440 |
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