Eugenol Inhibits Sodium Currents in Dental Afferent Neurons
Although eugenol is widely used in dentistry, little is known about the molecular mechanisms responsible for its anesthetic properties. In addition to calcium channels, recently demonstrated by our group, there could be another molecular target for eugenol. Using a whole-cell patch-clamp technique,...
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| Veröffentlicht in: | Journal of dental research 2006-10, Vol.85 (10), p.900-904 |
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| container_title | Journal of dental research |
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| creator | Park, C.-K. Li, H.Y. Yeon, K.-Y. Jung, S.J. Choi, S.-Y. Lee, S.J. Lee, S. Park, K. Kim, J.S. Oh, S.B. |
| description | Although eugenol is widely used in dentistry, little is known about the molecular mechanisms responsible for its anesthetic properties. In addition to calcium channels, recently demonstrated by our group, there could be another molecular target for eugenol. Using a whole-cell patch-clamp technique, we investigated the effect of eugenol on voltage-gated sodium channel currents (I
Na
) in rat dental primary afferent neurons identified by retrograde labeling with a fluorescent dye in maxillary molars. Eugenol inhibited action potentials and I
Na
in both capsaicin-sensitive and capsaicin-insensitive neurons. The pre-treatment with capsazepine, a competitive antagonist of transient receptor potential vanilloid 1 (TRPV1), failed to block the inhibitory effect of eugenol on I
Na
, suggesting no involvement of TRPV1. Two types of I
Na
, tetrodotoxin (TTX)-resistant and TTX-sensitive I
Na
, were inhibited by eugenol. Our results demonstrated that eugenol inhibits I
Na
in a TRPV1-independent manner. We suggest that I
Na
inhibition by eugenol contributes to its analgesic effect. |
| doi_str_mv | 10.1177/154405910608501005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68887870</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_154405910608501005</sage_id><sourcerecordid>68887870</sourcerecordid><originalsourceid>FETCH-LOGICAL-c507t-6723ea34f82039cae7867db128a90249befb46d2e8e6650d7d040d7a402e7c2c3</originalsourceid><addsrcrecordid>eNp9kEtLAzEUhYMotj7-gAsZXLgbe5PJ5IGrUqsWii7U9ZCZuVOnzKMmzcJ_b4YWCgpuktzDd88Jh5ArCneUSjmhKeeQagoCVAoUID0i40GMB_WYjAEYiyHh6YicObcGoJqp5JSMqNBaUabG5H7uV9j1TbToPuu83rrorS9r30Yzby12Ya676CE8TBNNqwoHLXpBb_vOXZCTyjQOL_f3Ofl4nL_PnuPl69NiNl3GRQpyGwvJEjQJrxSDRBcGpRKyzEO80cC4zrHKuSgZKhQihVKWwMNpODCUBSuSc3K7893Y_suj22Zt7QpsGtNh710mlFJSSQjgzS9w3Xvbhb9lDDSXPCQHiO2gwvbOWayyja1bY78zCtnQa_a317B0vXf2eYvlYWVfZAAmO8CZFR5i_7H8AShEfes</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>209474786</pqid></control><display><type>article</type><title>Eugenol Inhibits Sodium Currents in Dental Afferent Neurons</title><source>Access via SAGE</source><source>MEDLINE</source><creator>Park, C.-K. ; Li, H.Y. ; Yeon, K.-Y. ; Jung, S.J. ; Choi, S.-Y. ; Lee, S.J. ; Lee, S. ; Park, K. ; Kim, J.S. ; Oh, S.B.</creator><creatorcontrib>Park, C.-K. ; Li, H.Y. ; Yeon, K.-Y. ; Jung, S.J. ; Choi, S.-Y. ; Lee, S.J. ; Lee, S. ; Park, K. ; Kim, J.S. ; Oh, S.B.</creatorcontrib><description>Although eugenol is widely used in dentistry, little is known about the molecular mechanisms responsible for its anesthetic properties. In addition to calcium channels, recently demonstrated by our group, there could be another molecular target for eugenol. Using a whole-cell patch-clamp technique, we investigated the effect of eugenol on voltage-gated sodium channel currents (I
Na
) in rat dental primary afferent neurons identified by retrograde labeling with a fluorescent dye in maxillary molars. Eugenol inhibited action potentials and I
Na
in both capsaicin-sensitive and capsaicin-insensitive neurons. The pre-treatment with capsazepine, a competitive antagonist of transient receptor potential vanilloid 1 (TRPV1), failed to block the inhibitory effect of eugenol on I
Na
, suggesting no involvement of TRPV1. Two types of I
Na
, tetrodotoxin (TTX)-resistant and TTX-sensitive I
Na
, were inhibited by eugenol. Our results demonstrated that eugenol inhibits I
Na
in a TRPV1-independent manner. We suggest that I
Na
inhibition by eugenol contributes to its analgesic effect.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/154405910608501005</identifier><identifier>PMID: 16998128</identifier><identifier>CODEN: JDREAF</identifier><language>eng</language><publisher>United States: SAGE Publications</publisher><subject>Action Potentials - drug effects ; Anesthetics, Local - pharmacology ; Animals ; Cells, Cultured ; Dental Pulp - drug effects ; Dental Pulp - innervation ; Dentistry ; Eugenol - pharmacology ; Neurons, Afferent - classification ; Neurons, Afferent - drug effects ; Patch-Clamp Techniques ; Rats ; Rats, Sprague-Dawley ; Sodium Channel Blockers - pharmacology ; Sodium Channels - classification ; Sodium Channels - drug effects ; Trigeminal Ganglion - cytology ; Trigeminal Ganglion - drug effects</subject><ispartof>Journal of dental research, 2006-10, Vol.85 (10), p.900-904</ispartof><rights>International and American Associations for Dental Research</rights><rights>Copyright American Association for Dental Research/American Academy of Implant Dentistry Oct 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-6723ea34f82039cae7867db128a90249befb46d2e8e6650d7d040d7a402e7c2c3</citedby><cites>FETCH-LOGICAL-c507t-6723ea34f82039cae7867db128a90249befb46d2e8e6650d7d040d7a402e7c2c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/154405910608501005$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/154405910608501005$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16998128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, C.-K.</creatorcontrib><creatorcontrib>Li, H.Y.</creatorcontrib><creatorcontrib>Yeon, K.-Y.</creatorcontrib><creatorcontrib>Jung, S.J.</creatorcontrib><creatorcontrib>Choi, S.-Y.</creatorcontrib><creatorcontrib>Lee, S.J.</creatorcontrib><creatorcontrib>Lee, S.</creatorcontrib><creatorcontrib>Park, K.</creatorcontrib><creatorcontrib>Kim, J.S.</creatorcontrib><creatorcontrib>Oh, S.B.</creatorcontrib><title>Eugenol Inhibits Sodium Currents in Dental Afferent Neurons</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Although eugenol is widely used in dentistry, little is known about the molecular mechanisms responsible for its anesthetic properties. In addition to calcium channels, recently demonstrated by our group, there could be another molecular target for eugenol. Using a whole-cell patch-clamp technique, we investigated the effect of eugenol on voltage-gated sodium channel currents (I
Na
) in rat dental primary afferent neurons identified by retrograde labeling with a fluorescent dye in maxillary molars. Eugenol inhibited action potentials and I
Na
in both capsaicin-sensitive and capsaicin-insensitive neurons. The pre-treatment with capsazepine, a competitive antagonist of transient receptor potential vanilloid 1 (TRPV1), failed to block the inhibitory effect of eugenol on I
Na
, suggesting no involvement of TRPV1. Two types of I
Na
, tetrodotoxin (TTX)-resistant and TTX-sensitive I
Na
, were inhibited by eugenol. Our results demonstrated that eugenol inhibits I
Na
in a TRPV1-independent manner. We suggest that I
Na
inhibition by eugenol contributes to its analgesic effect.</description><subject>Action Potentials - drug effects</subject><subject>Anesthetics, Local - pharmacology</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Dental Pulp - drug effects</subject><subject>Dental Pulp - innervation</subject><subject>Dentistry</subject><subject>Eugenol - pharmacology</subject><subject>Neurons, Afferent - classification</subject><subject>Neurons, Afferent - drug effects</subject><subject>Patch-Clamp Techniques</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sodium Channel Blockers - pharmacology</subject><subject>Sodium Channels - classification</subject><subject>Sodium Channels - drug effects</subject><subject>Trigeminal Ganglion - cytology</subject><subject>Trigeminal Ganglion - drug effects</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEtLAzEUhYMotj7-gAsZXLgbe5PJ5IGrUqsWii7U9ZCZuVOnzKMmzcJ_b4YWCgpuktzDd88Jh5ArCneUSjmhKeeQagoCVAoUID0i40GMB_WYjAEYiyHh6YicObcGoJqp5JSMqNBaUabG5H7uV9j1TbToPuu83rrorS9r30Yzby12Ya676CE8TBNNqwoHLXpBb_vOXZCTyjQOL_f3Ofl4nL_PnuPl69NiNl3GRQpyGwvJEjQJrxSDRBcGpRKyzEO80cC4zrHKuSgZKhQihVKWwMNpODCUBSuSc3K7893Y_suj22Zt7QpsGtNh710mlFJSSQjgzS9w3Xvbhb9lDDSXPCQHiO2gwvbOWayyja1bY78zCtnQa_a317B0vXf2eYvlYWVfZAAmO8CZFR5i_7H8AShEfes</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Park, C.-K.</creator><creator>Li, H.Y.</creator><creator>Yeon, K.-Y.</creator><creator>Jung, S.J.</creator><creator>Choi, S.-Y.</creator><creator>Lee, S.J.</creator><creator>Lee, S.</creator><creator>Park, K.</creator><creator>Kim, J.S.</creator><creator>Oh, S.B.</creator><general>SAGE Publications</general><general>SAGE PUBLICATIONS, INC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>Eugenol Inhibits Sodium Currents in Dental Afferent Neurons</title><author>Park, C.-K. ; Li, H.Y. ; Yeon, K.-Y. ; Jung, S.J. ; Choi, S.-Y. ; Lee, S.J. ; Lee, S. ; Park, K. ; Kim, J.S. ; Oh, S.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-6723ea34f82039cae7867db128a90249befb46d2e8e6650d7d040d7a402e7c2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Action Potentials - drug effects</topic><topic>Anesthetics, Local - pharmacology</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Dental Pulp - drug effects</topic><topic>Dental Pulp - innervation</topic><topic>Dentistry</topic><topic>Eugenol - pharmacology</topic><topic>Neurons, Afferent - classification</topic><topic>Neurons, Afferent - drug effects</topic><topic>Patch-Clamp Techniques</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sodium Channel Blockers - pharmacology</topic><topic>Sodium Channels - classification</topic><topic>Sodium Channels - drug effects</topic><topic>Trigeminal Ganglion - cytology</topic><topic>Trigeminal Ganglion - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, C.-K.</creatorcontrib><creatorcontrib>Li, H.Y.</creatorcontrib><creatorcontrib>Yeon, K.-Y.</creatorcontrib><creatorcontrib>Jung, S.J.</creatorcontrib><creatorcontrib>Choi, S.-Y.</creatorcontrib><creatorcontrib>Lee, S.J.</creatorcontrib><creatorcontrib>Lee, S.</creatorcontrib><creatorcontrib>Park, K.</creatorcontrib><creatorcontrib>Kim, J.S.</creatorcontrib><creatorcontrib>Oh, S.B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, C.-K.</au><au>Li, H.Y.</au><au>Yeon, K.-Y.</au><au>Jung, S.J.</au><au>Choi, S.-Y.</au><au>Lee, S.J.</au><au>Lee, S.</au><au>Park, K.</au><au>Kim, J.S.</au><au>Oh, S.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Eugenol Inhibits Sodium Currents in Dental Afferent Neurons</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>85</volume><issue>10</issue><spage>900</spage><epage>904</epage><pages>900-904</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><coden>JDREAF</coden><abstract>Although eugenol is widely used in dentistry, little is known about the molecular mechanisms responsible for its anesthetic properties. In addition to calcium channels, recently demonstrated by our group, there could be another molecular target for eugenol. Using a whole-cell patch-clamp technique, we investigated the effect of eugenol on voltage-gated sodium channel currents (I
Na
) in rat dental primary afferent neurons identified by retrograde labeling with a fluorescent dye in maxillary molars. Eugenol inhibited action potentials and I
Na
in both capsaicin-sensitive and capsaicin-insensitive neurons. The pre-treatment with capsazepine, a competitive antagonist of transient receptor potential vanilloid 1 (TRPV1), failed to block the inhibitory effect of eugenol on I
Na
, suggesting no involvement of TRPV1. Two types of I
Na
, tetrodotoxin (TTX)-resistant and TTX-sensitive I
Na
, were inhibited by eugenol. Our results demonstrated that eugenol inhibits I
Na
in a TRPV1-independent manner. We suggest that I
Na
inhibition by eugenol contributes to its analgesic effect.</abstract><cop>United States</cop><pub>SAGE Publications</pub><pmid>16998128</pmid><doi>10.1177/154405910608501005</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Action Potentials - drug effects Anesthetics, Local - pharmacology Animals Cells, Cultured Dental Pulp - drug effects Dental Pulp - innervation Dentistry Eugenol - pharmacology Neurons, Afferent - classification Neurons, Afferent - drug effects Patch-Clamp Techniques Rats Rats, Sprague-Dawley Sodium Channel Blockers - pharmacology Sodium Channels - classification Sodium Channels - drug effects Trigeminal Ganglion - cytology Trigeminal Ganglion - drug effects |
| title | Eugenol Inhibits Sodium Currents in Dental Afferent Neurons |
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