The enigma of ATCE1, an acrosome-associated transcription factor
Atce1 belongs to the CREB3/LZIP subtype of the ATF/CREB transcription factor gene family. Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and...
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Veröffentlicht in: | Developmental biology 2006-10, Vol.298 (1), p.201-211 |
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description | Atce1 belongs to the CREB3/LZIP subtype of the ATF/CREB transcription factor gene family. Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and found that it accumulates in late round and in elongating spermatids, corresponding to developmental stages considered transcriptionally silent. ATCE1 accumulation is acrosome-specific and persists up to mature epididymal cells, at which stage the protein remained associated with the inner acrosome membrane even after acrosomal reaction. No nuclear localization was evident at any spermatogenic stage. Expression of full-length ATCE1 in various cell lines revealed ER and Golgi localization whereas truncation of the C-terminus allowed entrance into the nucleus. Potent transcriptional activation activity, from kB-containing regulatory elements (but not from CRE elements as one might expect), was observed using the C-terminally truncated nuclear form of ATCE1. These results raise the question of why would a transcription factor be specifically anchored to the acrosome inner membrane? An intriguing speculation that ATCE1 might be paternally delivered to the newly formed zygote is discussed. |
doi_str_mv | 10.1016/j.ydbio.2006.06.029 |
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Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and found that it accumulates in late round and in elongating spermatids, corresponding to developmental stages considered transcriptionally silent. ATCE1 accumulation is acrosome-specific and persists up to mature epididymal cells, at which stage the protein remained associated with the inner acrosome membrane even after acrosomal reaction. No nuclear localization was evident at any spermatogenic stage. Expression of full-length ATCE1 in various cell lines revealed ER and Golgi localization whereas truncation of the C-terminus allowed entrance into the nucleus. Potent transcriptional activation activity, from kB-containing regulatory elements (but not from CRE elements as one might expect), was observed using the C-terminally truncated nuclear form of ATCE1. These results raise the question of why would a transcription factor be specifically anchored to the acrosome inner membrane? An intriguing speculation that ATCE1 might be paternally delivered to the newly formed zygote is discussed.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/j.ydbio.2006.06.029</identifier><identifier>PMID: 16925989</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acrosome ; Acrosome - metabolism ; Animals ; ATCE1 ; Cyclic AMP Response Element-Binding Protein - genetics ; Cyclic AMP Response Element-Binding Protein - metabolism ; HeLa Cells ; Humans ; Male ; Mice ; Models, Biological ; NIH 3T3 Cells ; Spermatid ; Testis - growth & development ; Testis - metabolism ; Tissue Distribution ; Trans-Activators - physiology ; Transcription factor ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transfection ; Zygotic gene activation</subject><ispartof>Developmental biology, 2006-10, Vol.298 (1), p.201-211</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-8d34e468913226efc8d4f2e7f85bdc2b0e0624cdd02d7e1f1bacc16ae0d989a63</citedby><cites>FETCH-LOGICAL-c499t-8d34e468913226efc8d4f2e7f85bdc2b0e0624cdd02d7e1f1bacc16ae0d989a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ydbio.2006.06.029$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16925989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gil, Stelzer</creatorcontrib><creatorcontrib>Yosef, Dicken</creatorcontrib><creatorcontrib>Golan, Niv</creatorcontrib><creatorcontrib>Don, Jeremy</creatorcontrib><title>The enigma of ATCE1, an acrosome-associated transcription factor</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Atce1 belongs to the CREB3/LZIP subtype of the ATF/CREB transcription factor gene family. Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and found that it accumulates in late round and in elongating spermatids, corresponding to developmental stages considered transcriptionally silent. ATCE1 accumulation is acrosome-specific and persists up to mature epididymal cells, at which stage the protein remained associated with the inner acrosome membrane even after acrosomal reaction. No nuclear localization was evident at any spermatogenic stage. Expression of full-length ATCE1 in various cell lines revealed ER and Golgi localization whereas truncation of the C-terminus allowed entrance into the nucleus. Potent transcriptional activation activity, from kB-containing regulatory elements (but not from CRE elements as one might expect), was observed using the C-terminally truncated nuclear form of ATCE1. These results raise the question of why would a transcription factor be specifically anchored to the acrosome inner membrane? An intriguing speculation that ATCE1 might be paternally delivered to the newly formed zygote is discussed.</description><subject>Acrosome</subject><subject>Acrosome - metabolism</subject><subject>Animals</subject><subject>ATCE1</subject><subject>Cyclic AMP Response Element-Binding Protein - genetics</subject><subject>Cyclic AMP Response Element-Binding Protein - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Male</subject><subject>Mice</subject><subject>Models, Biological</subject><subject>NIH 3T3 Cells</subject><subject>Spermatid</subject><subject>Testis - growth & development</subject><subject>Testis - metabolism</subject><subject>Tissue Distribution</subject><subject>Trans-Activators - physiology</subject><subject>Transcription factor</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transfection</subject><subject>Zygotic gene activation</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotlZ_gSB78uTWfG2aHARLqR9Q8FLBW8gms5rS3dRkK_Tfu2sL3hQG5vLMOzMPQpcEjwkm4nY13rnShzHFWIz7ouoIDQlWRV4I_naMhhgTmhOBxQCdpbTCGDMp2SkaEKFooaQaovvlB2TQ-PfaZKHKpsvZnNxkpsmMjSGFGnKTUrDetOCyNpom2eg3rQ9NVhnbhniOTiqzTnBx6CP0-jBfzp7yxcvj82y6yC1Xqs2lYxy4kIowSgVUVjpeUZhUsiidpSUGLCi3zmHqJkAqUhpriTCAXXenEWyErve5mxg-t5BaXftkYb02DYRt0kJKOeFc_QsSxRihgnUg24P9pylCpTfR1ybuNMG6N6xX-sew7g3rvmgff3WI35Y1uN-Zg9IOuNsD0Nn48hB1sh4aC85HsK12wf-54BuMqY06</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Gil, Stelzer</creator><creator>Yosef, Dicken</creator><creator>Golan, Niv</creator><creator>Don, Jeremy</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>The enigma of ATCE1, an acrosome-associated transcription factor</title><author>Gil, Stelzer ; Yosef, Dicken ; Golan, Niv ; Don, Jeremy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-8d34e468913226efc8d4f2e7f85bdc2b0e0624cdd02d7e1f1bacc16ae0d989a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acrosome</topic><topic>Acrosome - metabolism</topic><topic>Animals</topic><topic>ATCE1</topic><topic>Cyclic AMP Response Element-Binding Protein - genetics</topic><topic>Cyclic AMP Response Element-Binding Protein - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Male</topic><topic>Mice</topic><topic>Models, Biological</topic><topic>NIH 3T3 Cells</topic><topic>Spermatid</topic><topic>Testis - growth & development</topic><topic>Testis - metabolism</topic><topic>Tissue Distribution</topic><topic>Trans-Activators - physiology</topic><topic>Transcription factor</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transfection</topic><topic>Zygotic gene activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gil, Stelzer</creatorcontrib><creatorcontrib>Yosef, Dicken</creatorcontrib><creatorcontrib>Golan, Niv</creatorcontrib><creatorcontrib>Don, Jeremy</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gil, Stelzer</au><au>Yosef, Dicken</au><au>Golan, Niv</au><au>Don, Jeremy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The enigma of ATCE1, an acrosome-associated transcription factor</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>298</volume><issue>1</issue><spage>201</spage><epage>211</epage><pages>201-211</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Atce1 belongs to the CREB3/LZIP subtype of the ATF/CREB transcription factor gene family. Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and found that it accumulates in late round and in elongating spermatids, corresponding to developmental stages considered transcriptionally silent. ATCE1 accumulation is acrosome-specific and persists up to mature epididymal cells, at which stage the protein remained associated with the inner acrosome membrane even after acrosomal reaction. No nuclear localization was evident at any spermatogenic stage. Expression of full-length ATCE1 in various cell lines revealed ER and Golgi localization whereas truncation of the C-terminus allowed entrance into the nucleus. Potent transcriptional activation activity, from kB-containing regulatory elements (but not from CRE elements as one might expect), was observed using the C-terminally truncated nuclear form of ATCE1. 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subjects | Acrosome Acrosome - metabolism Animals ATCE1 Cyclic AMP Response Element-Binding Protein - genetics Cyclic AMP Response Element-Binding Protein - metabolism HeLa Cells Humans Male Mice Models, Biological NIH 3T3 Cells Spermatid Testis - growth & development Testis - metabolism Tissue Distribution Trans-Activators - physiology Transcription factor Transcription Factors - genetics Transcription Factors - metabolism Transfection Zygotic gene activation |
title | The enigma of ATCE1, an acrosome-associated transcription factor |
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