Staphylococcus aureus small colony variants are resistant to the antimicrobial peptide lactoferricin B
Objectives: To determine whether Staphylococcus aureus small colony variants (SCVs) are resistant to the antimicrobial peptide lactoferricin B. To assess if deficiency in transmembrane potential, a common characteristic of SCVs that are haemin- or menadione-auxotrophs, affects the uptake of the pept...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2005-12, Vol.56 (6), p.1126-1129 |
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| container_title | Journal of antimicrobial chemotherapy |
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| creator | Samuelsen, Ørjan Haukland, Hanne Husom Kahl, Barbara C. von Eiff, Christof Proctor, Richard A. Ulvatne, Hilde Sandvik, Kjersti Vorland, Lars H. |
| description | Objectives: To determine whether Staphylococcus aureus small colony variants (SCVs) are resistant to the antimicrobial peptide lactoferricin B. To assess if deficiency in transmembrane potential, a common characteristic of SCVs that are haemin- or menadione-auxotrophs, affects the uptake of the peptide into the bacterial cytoplasm. Methods: A broth microdilution technique was used for susceptibility testing to determine the MIC of lactoferricin B for SCVs with three different auxotrophisms (haemin, menadione or thymidine) and their isogenic parent strains. Both clinical isolates and genetically defined mutants were used. The internalization of lactoferricin B in a hemB mutant and the respective parent strain was studied using transmission electron microscopy and immunogold labelling. Results: All SCVs showed reduced susceptibility to lactoferricin B irrespective of their auxotrophy compared with their isogenic parent strains. The MIC for all SCVs was >256 mg/L, whereas the MICs for the parent strains ranged from 16–256 mg/L. Surprisingly, the hemB mutant contained significantly more lactoferricin B intracellularly than the respective parent strain. Conclusions: The resistance mechanism of SCVs towards the antimicrobial peptide lactoferricin B is presumably caused by the metabolic changes present in SCVs rather than by a changed transmembrane potential of SCVs or reduced uptake of the peptide. |
| doi_str_mv | 10.1093/jac/dki385 |
| format | Article |
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To assess if deficiency in transmembrane potential, a common characteristic of SCVs that are haemin- or menadione-auxotrophs, affects the uptake of the peptide into the bacterial cytoplasm. Methods: A broth microdilution technique was used for susceptibility testing to determine the MIC of lactoferricin B for SCVs with three different auxotrophisms (haemin, menadione or thymidine) and their isogenic parent strains. Both clinical isolates and genetically defined mutants were used. The internalization of lactoferricin B in a hemB mutant and the respective parent strain was studied using transmission electron microscopy and immunogold labelling. Results: All SCVs showed reduced susceptibility to lactoferricin B irrespective of their auxotrophy compared with their isogenic parent strains. The MIC for all SCVs was >256 mg/L, whereas the MICs for the parent strains ranged from 16–256 mg/L. Surprisingly, the hemB mutant contained significantly more lactoferricin B intracellularly than the respective parent strain. Conclusions: The resistance mechanism of SCVs towards the antimicrobial peptide lactoferricin B is presumably caused by the metabolic changes present in SCVs rather than by a changed transmembrane potential of SCVs or reduced uptake of the peptide.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dki385</identifier><identifier>PMID: 16287983</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Anti-Bacterial Agents - metabolism ; Anti-Bacterial Agents - pharmacology ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; antimicrobial resistance ; Biological and medical sciences ; cellular uptake ; Cytoplasm - chemistry ; Drug Resistance, Bacterial ; electron microscopy ; Hemin - genetics ; immunolabelling ; Lactoferrin - metabolism ; Lactoferrin - pharmacology ; Medical sciences ; Membrane Potentials ; metabolic resistance ; Microbial Sensitivity Tests ; Microscopy, Electron, Transmission ; Microscopy, Immunoelectron ; Mutation ; Pharmacology. Drug treatments ; resistance mechanism ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - growth & development ; Thymidine - genetics ; transmembrane potential ; Vitamin K 3 - metabolism</subject><ispartof>Journal of antimicrobial chemotherapy, 2005-12, Vol.56 (6), p.1126-1129</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Dec 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-6fc2694b346d06947f195d973d02d67e524331d0291a23fc11613921880a1b2a3</citedby><cites>FETCH-LOGICAL-c513t-6fc2694b346d06947f195d973d02d67e524331d0291a23fc11613921880a1b2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17379201$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16287983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Samuelsen, Ørjan</creatorcontrib><creatorcontrib>Haukland, Hanne Husom</creatorcontrib><creatorcontrib>Kahl, Barbara C.</creatorcontrib><creatorcontrib>von Eiff, Christof</creatorcontrib><creatorcontrib>Proctor, Richard A.</creatorcontrib><creatorcontrib>Ulvatne, Hilde</creatorcontrib><creatorcontrib>Sandvik, Kjersti</creatorcontrib><creatorcontrib>Vorland, Lars H.</creatorcontrib><title>Staphylococcus aureus small colony variants are resistant to the antimicrobial peptide lactoferricin B</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J. Antimicrob. Chemother</addtitle><description>Objectives: To determine whether Staphylococcus aureus small colony variants (SCVs) are resistant to the antimicrobial peptide lactoferricin B. To assess if deficiency in transmembrane potential, a common characteristic of SCVs that are haemin- or menadione-auxotrophs, affects the uptake of the peptide into the bacterial cytoplasm. Methods: A broth microdilution technique was used for susceptibility testing to determine the MIC of lactoferricin B for SCVs with three different auxotrophisms (haemin, menadione or thymidine) and their isogenic parent strains. Both clinical isolates and genetically defined mutants were used. The internalization of lactoferricin B in a hemB mutant and the respective parent strain was studied using transmission electron microscopy and immunogold labelling. Results: All SCVs showed reduced susceptibility to lactoferricin B irrespective of their auxotrophy compared with their isogenic parent strains. The MIC for all SCVs was >256 mg/L, whereas the MICs for the parent strains ranged from 16–256 mg/L. Surprisingly, the hemB mutant contained significantly more lactoferricin B intracellularly than the respective parent strain. Conclusions: The resistance mechanism of SCVs towards the antimicrobial peptide lactoferricin B is presumably caused by the metabolic changes present in SCVs rather than by a changed transmembrane potential of SCVs or reduced uptake of the peptide.</description><subject>Anti-Bacterial Agents - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>antimicrobial resistance</subject><subject>Biological and medical sciences</subject><subject>cellular uptake</subject><subject>Cytoplasm - chemistry</subject><subject>Drug Resistance, Bacterial</subject><subject>electron microscopy</subject><subject>Hemin - genetics</subject><subject>immunolabelling</subject><subject>Lactoferrin - metabolism</subject><subject>Lactoferrin - pharmacology</subject><subject>Medical sciences</subject><subject>Membrane Potentials</subject><subject>metabolic resistance</subject><subject>Microbial Sensitivity Tests</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Immunoelectron</subject><subject>Mutation</subject><subject>Pharmacology. Drug treatments</subject><subject>resistance mechanism</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - growth & development</subject><subject>Thymidine - genetics</subject><subject>transmembrane potential</subject><subject>Vitamin K 3 - metabolism</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U2LFDEQBuAgiju7evEHSBD0ILSbSrrzcdTFcYQFhVERLyGTTrOZTXfaJC3Ov9_IDC548VRV1ENB8iL0DMgbIIpd7o297G89k90DtIKWk4YSBQ_RijDSNaLt2Bk6z3lPCOEdl4_RGXAqhZJshYZtMfPNIUQbrV0yNktyteTRhIBtDHE64F8meTOVukwOJ5d9LnXEJeJy43Bt_ehtijtvAp7dXHzvcDC2xMGl5K2f8Lsn6NFgQnZPT_UCfV2__3K1aa4_ffh49fa6sR2w0vDBUq7aHWt5T2ojBlBdrwTrCe25cB1tGYM6KDCUDRaAA1MUpCQGdtSwC_TqeHdO8efictGjz9aFYCYXl6y5lJIrKv8LQbSUEckrfPEP3MclTfURmoLgUkjFKnp9RPUbck5u0HPyo0kHDUT_yUjXjPQxo4qfny4uu9H19_QUSgUvT8Bka8KQzGR9vneCCUUJVNccXQ3E_f67N-lW82o6vfn-Q39bk-12LTb6M7sDfLGpNg</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Samuelsen, Ørjan</creator><creator>Haukland, Hanne Husom</creator><creator>Kahl, Barbara C.</creator><creator>von Eiff, Christof</creator><creator>Proctor, Richard A.</creator><creator>Ulvatne, Hilde</creator><creator>Sandvik, Kjersti</creator><creator>Vorland, Lars H.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Staphylococcus aureus small colony variants are resistant to the antimicrobial peptide lactoferricin B</title><author>Samuelsen, Ørjan ; Haukland, Hanne Husom ; Kahl, Barbara C. ; von Eiff, Christof ; Proctor, Richard A. ; Ulvatne, Hilde ; Sandvik, Kjersti ; Vorland, Lars H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-6fc2694b346d06947f195d973d02d67e524331d0291a23fc11613921880a1b2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Anti-Bacterial Agents - metabolism</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>antimicrobial resistance</topic><topic>Biological and medical sciences</topic><topic>cellular uptake</topic><topic>Cytoplasm - chemistry</topic><topic>Drug Resistance, Bacterial</topic><topic>electron microscopy</topic><topic>Hemin - genetics</topic><topic>immunolabelling</topic><topic>Lactoferrin - metabolism</topic><topic>Lactoferrin - pharmacology</topic><topic>Medical sciences</topic><topic>Membrane Potentials</topic><topic>metabolic resistance</topic><topic>Microbial Sensitivity Tests</topic><topic>Microscopy, Electron, Transmission</topic><topic>Microscopy, Immunoelectron</topic><topic>Mutation</topic><topic>Pharmacology. Drug treatments</topic><topic>resistance mechanism</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - growth & development</topic><topic>Thymidine - genetics</topic><topic>transmembrane potential</topic><topic>Vitamin K 3 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samuelsen, Ørjan</creatorcontrib><creatorcontrib>Haukland, Hanne Husom</creatorcontrib><creatorcontrib>Kahl, Barbara C.</creatorcontrib><creatorcontrib>von Eiff, Christof</creatorcontrib><creatorcontrib>Proctor, Richard A.</creatorcontrib><creatorcontrib>Ulvatne, Hilde</creatorcontrib><creatorcontrib>Sandvik, Kjersti</creatorcontrib><creatorcontrib>Vorland, Lars H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Samuelsen, Ørjan</au><au>Haukland, Hanne Husom</au><au>Kahl, Barbara C.</au><au>von Eiff, Christof</au><au>Proctor, Richard A.</au><au>Ulvatne, Hilde</au><au>Sandvik, Kjersti</au><au>Vorland, Lars H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Staphylococcus aureus small colony variants are resistant to the antimicrobial peptide lactoferricin B</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J. Antimicrob. Chemother</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>56</volume><issue>6</issue><spage>1126</spage><epage>1129</epage><pages>1126-1129</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives: To determine whether Staphylococcus aureus small colony variants (SCVs) are resistant to the antimicrobial peptide lactoferricin B. To assess if deficiency in transmembrane potential, a common characteristic of SCVs that are haemin- or menadione-auxotrophs, affects the uptake of the peptide into the bacterial cytoplasm. Methods: A broth microdilution technique was used for susceptibility testing to determine the MIC of lactoferricin B for SCVs with three different auxotrophisms (haemin, menadione or thymidine) and their isogenic parent strains. Both clinical isolates and genetically defined mutants were used. The internalization of lactoferricin B in a hemB mutant and the respective parent strain was studied using transmission electron microscopy and immunogold labelling. Results: All SCVs showed reduced susceptibility to lactoferricin B irrespective of their auxotrophy compared with their isogenic parent strains. The MIC for all SCVs was >256 mg/L, whereas the MICs for the parent strains ranged from 16–256 mg/L. Surprisingly, the hemB mutant contained significantly more lactoferricin B intracellularly than the respective parent strain. Conclusions: The resistance mechanism of SCVs towards the antimicrobial peptide lactoferricin B is presumably caused by the metabolic changes present in SCVs rather than by a changed transmembrane potential of SCVs or reduced uptake of the peptide.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>16287983</pmid><doi>10.1093/jac/dki385</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology Antibiotics. Antiinfectious agents. Antiparasitic agents antimicrobial resistance Biological and medical sciences cellular uptake Cytoplasm - chemistry Drug Resistance, Bacterial electron microscopy Hemin - genetics immunolabelling Lactoferrin - metabolism Lactoferrin - pharmacology Medical sciences Membrane Potentials metabolic resistance Microbial Sensitivity Tests Microscopy, Electron, Transmission Microscopy, Immunoelectron Mutation Pharmacology. Drug treatments resistance mechanism Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - growth & development Thymidine - genetics transmembrane potential Vitamin K 3 - metabolism |
| title | Staphylococcus aureus small colony variants are resistant to the antimicrobial peptide lactoferricin B |
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