The translational and transcriptional initiation sites of BmNPV lef-7 gene
The predicted open reading frame of lef-7 from Bombyx mori nucleopolyhedrovirus (BmNPV) is 45 bp longer at the 5'-terminal and harbors a 42 bp deletion towards the 3' terminal end compared to that of Autograph californica mlulticapsid NPV (AcMNPV). In the present study, to determine whethe...
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| Veröffentlicht in: | Virus genes 2006-12, Vol.33 (3), p.351-357 |
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| creator | Wang, Fang Yang, Li-Rong Tang, Xu-Dong Mo, Jian-Chu Yang, Wei-Jun Zhang, Chuan-Xi |
| description | The predicted open reading frame of lef-7 from Bombyx mori nucleopolyhedrovirus (BmNPV) is 45 bp longer at the 5'-terminal and harbors a 42 bp deletion towards the 3' terminal end compared to that of Autograph californica mlulticapsid NPV (AcMNPV). In the present study, to determine whether the BmNPV lef-7 is translated from an initiation site different from that of AcMNPV lef-7, the translational and transcriptional initiation sites of BmNPV lef-7 were examined. A BmNPV mutant, Bmlef7M1(-) was constructed by deleting 11 nucleotides (nt) including the predicted initiation codon ATG. Western blot analysis demonstrated that the size of LEF-7 in BmNPV and Bmlef7M1(-)-infected cells was identical. The LEF-7s in BmNPV and Bmlef7M1(-)-infected cells were both localized in the nuclei as observed using confocal microscopy. Therefore, the presumed initiation codon ATG (at 97059 nt of BmNPV genome) appears to be non-functional for lef-7 translation. The 5'-RACE analysis revealed that transcription of lef-7 mRNA in BmNPV and Bmlef7M1(-)-infected cells both initiated from an ATCATT motif located 26 nt upstream of the second ATG (located at 97014 nt on BmNPV genome), and 20 nt downstream of the presumed initiation codon. |
| doi_str_mv | 10.1007/s11262-006-0075-7 |
| format | Article |
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In the present study, to determine whether the BmNPV lef-7 is translated from an initiation site different from that of AcMNPV lef-7, the translational and transcriptional initiation sites of BmNPV lef-7 were examined. A BmNPV mutant, Bmlef7M1(-) was constructed by deleting 11 nucleotides (nt) including the predicted initiation codon ATG. Western blot analysis demonstrated that the size of LEF-7 in BmNPV and Bmlef7M1(-)-infected cells was identical. The LEF-7s in BmNPV and Bmlef7M1(-)-infected cells were both localized in the nuclei as observed using confocal microscopy. Therefore, the presumed initiation codon ATG (at 97059 nt of BmNPV genome) appears to be non-functional for lef-7 translation. The 5'-RACE analysis revealed that transcription of lef-7 mRNA in BmNPV and Bmlef7M1(-)-infected cells both initiated from an ATCATT motif located 26 nt upstream of the second ATG (located at 97014 nt on BmNPV genome), and 20 nt downstream of the presumed initiation codon.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-006-0075-7</identifier><identifier>PMID: 16991007</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Animals ; Bombyx - virology ; Bombyx mori ; Bombyx mori NPV ; Cell Line ; Codon, Initiator ; Gene Expression Regulation, Viral ; Nuclear polyhedrosis virus ; Nucleopolyhedrovirus - genetics ; Transcription, Genetic ; Viral Proteins - chemistry ; Viral Proteins - genetics ; Viral Proteins - metabolism</subject><ispartof>Virus genes, 2006-12, Vol.33 (3), p.351-357</ispartof><rights>Springer Science+Business Media, LLC 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16991007$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Yang, Li-Rong</creatorcontrib><creatorcontrib>Tang, Xu-Dong</creatorcontrib><creatorcontrib>Mo, Jian-Chu</creatorcontrib><creatorcontrib>Yang, Wei-Jun</creatorcontrib><creatorcontrib>Zhang, Chuan-Xi</creatorcontrib><title>The translational and transcriptional initiation sites of BmNPV lef-7 gene</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><description>The predicted open reading frame of lef-7 from Bombyx mori nucleopolyhedrovirus (BmNPV) is 45 bp longer at the 5'-terminal and harbors a 42 bp deletion towards the 3' terminal end compared to that of Autograph californica mlulticapsid NPV (AcMNPV). In the present study, to determine whether the BmNPV lef-7 is translated from an initiation site different from that of AcMNPV lef-7, the translational and transcriptional initiation sites of BmNPV lef-7 were examined. A BmNPV mutant, Bmlef7M1(-) was constructed by deleting 11 nucleotides (nt) including the predicted initiation codon ATG. Western blot analysis demonstrated that the size of LEF-7 in BmNPV and Bmlef7M1(-)-infected cells was identical. The LEF-7s in BmNPV and Bmlef7M1(-)-infected cells were both localized in the nuclei as observed using confocal microscopy. Therefore, the presumed initiation codon ATG (at 97059 nt of BmNPV genome) appears to be non-functional for lef-7 translation. The 5'-RACE analysis revealed that transcription of lef-7 mRNA in BmNPV and Bmlef7M1(-)-infected cells both initiated from an ATCATT motif located 26 nt upstream of the second ATG (located at 97014 nt on BmNPV genome), and 20 nt downstream of the presumed initiation codon.</description><subject>Animals</subject><subject>Bombyx - virology</subject><subject>Bombyx mori</subject><subject>Bombyx mori NPV</subject><subject>Cell Line</subject><subject>Codon, Initiator</subject><subject>Gene Expression Regulation, Viral</subject><subject>Nuclear polyhedrosis virus</subject><subject>Nucleopolyhedrovirus - genetics</subject><subject>Transcription, Genetic</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0LtOwzAUBmALgWgpPAALihjYDL4e2yNU5aYKGApii-zEAVe5EScDb0-gZWFhODrSr0-_dA5Cx5ScU0LURaSUAcOEwDhKYrWDplQqho0Rr7toSgwjWEswE3QQ45oQojUT-2hCwZjvhim6X737pO9sHUvbh6a2ZWLrfJNkXWi3WahDH35AEkPvY9IUyVX18PSSlL7AKnnztT9Ee4Utoz_a7hl6vl6s5rd4-XhzN79c4paB6rGnvnC5I1KCA0FtTgQI4DIzptCGc0W1dVbLQgnOVG4My5wC4UE77VTG-QydbXrbrvkYfOzTKsTMl6WtfTPEFLTWHKj8F1Ijx3aiR3j6B66boRvvjiljHBSjko7oZIsGV_k8bbtQ2e4z_f0l_wIVTXS5</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Wang, Fang</creator><creator>Yang, Li-Rong</creator><creator>Tang, Xu-Dong</creator><creator>Mo, Jian-Chu</creator><creator>Yang, Wei-Jun</creator><creator>Zhang, Chuan-Xi</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>The translational and transcriptional initiation sites of BmNPV lef-7 gene</title><author>Wang, Fang ; 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In the present study, to determine whether the BmNPV lef-7 is translated from an initiation site different from that of AcMNPV lef-7, the translational and transcriptional initiation sites of BmNPV lef-7 were examined. A BmNPV mutant, Bmlef7M1(-) was constructed by deleting 11 nucleotides (nt) including the predicted initiation codon ATG. Western blot analysis demonstrated that the size of LEF-7 in BmNPV and Bmlef7M1(-)-infected cells was identical. The LEF-7s in BmNPV and Bmlef7M1(-)-infected cells were both localized in the nuclei as observed using confocal microscopy. Therefore, the presumed initiation codon ATG (at 97059 nt of BmNPV genome) appears to be non-functional for lef-7 translation. The 5'-RACE analysis revealed that transcription of lef-7 mRNA in BmNPV and Bmlef7M1(-)-infected cells both initiated from an ATCATT motif located 26 nt upstream of the second ATG (located at 97014 nt on BmNPV genome), and 20 nt downstream of the presumed initiation codon.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>16991007</pmid><doi>10.1007/s11262-006-0075-7</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Bombyx - virology Bombyx mori Bombyx mori NPV Cell Line Codon, Initiator Gene Expression Regulation, Viral Nuclear polyhedrosis virus Nucleopolyhedrovirus - genetics Transcription, Genetic Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - metabolism |
| title | The translational and transcriptional initiation sites of BmNPV lef-7 gene |
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