Response to Zhang et al. (2005): loss-of-function mutation in tryptophan hydroxylase-2 identified in unipolar major depression. Neuron 45, 11-16

Response to Zhang et al. (2005): loss-of-function mutation in tryptophan hydroxylase-2 identified in unipolar major depression. Neuron 45, 11-16

For direct sequencing, genomic DNA was amplified by PCR with primers encompassing the Arg441His variant, sequenced using the BigDye Terminator V3.1 (Applied Biosystems Inc., Foster City, CA) and analyzed on ABI 3100 or 3730 sequencers. [...]the His441 allele was readily detectable by both sequencing...

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Veröffentlicht in:Neuron (Cambridge, Mass.) Mass.), 2005-12, Vol.48 (5), p.702-703
Hauptverfasser: Zhou, Zhifeng, Peters, Eric J, Hamilton, Steven P, McMahon, Francis, Thomas, Christee, McGrath, Patrick J, Rush, John, Trivedi, Madhukar H, Charney, Dennis S, Roy, Alec, Wisniewski, Stephen, Lipsky, Robert, Goldman, David
Format: Artikel
Sprache:eng
Schlagworte:
African Americans
Binomial distribution
Deoxyribonucleic acid
Depressive Disorder, Major - genetics
DNA
Genes
Humans
Mental depression
Mutation
Native North Americans
Probability
Tryptophan Hydroxylase - genetics
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Zusammenfassung:For direct sequencing, genomic DNA was amplified by PCR with primers encompassing the Arg441His variant, sequenced using the BigDye Terminator V3.1 (Applied Biosystems Inc., Foster City, CA) and analyzed on ABI 3100 or 3730 sequencers. [...]the His441 allele was readily detectable by both sequencing and genotyping in positive control heterozygous DNA.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2005.11.018