Glutamate metabolizing enzymes in prefrontal cortex of Alzheimer's disease patients
Amounts of glutamate metabolizing enzymes such as glutamate dehydrogenase (GDH), glutamine synthetase (GS), GS-like protein (GSLP), and phosphate-activated glutaminase (PAG) were compared in prefrontal cortex of control subjects and patients with Alzheimer disease (AD). The target proteins were quan...
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| Veröffentlicht in: | Neurochemical research 2005-11, Vol.30 (11), p.1443-1451 |
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| container_title | Neurochemical research |
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| creator | Burbaeva, Gulnur Sh Boksha, Irina S Tereshkina, Elena B Savushkina, Olga K Starodubtseva, Lubov' I Turishcheva, Marina S |
| description | Amounts of glutamate metabolizing enzymes such as glutamate dehydrogenase (GDH), glutamine synthetase (GS), GS-like protein (GSLP), and phosphate-activated glutaminase (PAG) were compared in prefrontal cortex of control subjects and patients with Alzheimer disease (AD). The target proteins were quantified by ECL-Western immunoblotting in extracts from brain tissue prepared by two different techniques separating enzymes preferentially associated with cytoplasm (GDH I and II isoenzymes, GS, and partially GSLP) and membrane (GDH III, PAG, and partially GSLP) fractions. Amounts of all listed enzymes were found significantly increased in the patient group compared with controls. Some links between the measured values were observed in the control, but not in the AD patient group. The results may suggest for the pathological interruption of regulatory relations between distinct enzymes of glutamate metabolism in brain of AD patients. |
| doi_str_mv | 10.1007/s11064-005-8654-x |
| format | Article |
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The target proteins were quantified by ECL-Western immunoblotting in extracts from brain tissue prepared by two different techniques separating enzymes preferentially associated with cytoplasm (GDH I and II isoenzymes, GS, and partially GSLP) and membrane (GDH III, PAG, and partially GSLP) fractions. Amounts of all listed enzymes were found significantly increased in the patient group compared with controls. Some links between the measured values were observed in the control, but not in the AD patient group. The results may suggest for the pathological interruption of regulatory relations between distinct enzymes of glutamate metabolism in brain of AD patients.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-005-8654-x</identifier><identifier>PMID: 16341942</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Aged ; Alzheimer Disease - enzymology ; Amide Synthases - metabolism ; Female ; Glutamate Dehydrogenase - metabolism ; Glutamate-Ammonia Ligase - metabolism ; Glutamic Acid - metabolism ; Glutaminase - metabolism ; Humans ; Isoenzymes - metabolism ; Male ; Middle Aged ; Nerve Tissue Proteins - metabolism ; Prefrontal Cortex - enzymology ; Statistics as Topic ; Tissue Extracts - metabolism</subject><ispartof>Neurochemical research, 2005-11, Vol.30 (11), p.1443-1451</ispartof><rights>Springer Science+Business Media, Inc. 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-e51468d0309718aa24665d41ea3bb2ef4356fedc07fecec8a9abee523de53aef3</citedby><cites>FETCH-LOGICAL-c423t-e51468d0309718aa24665d41ea3bb2ef4356fedc07fecec8a9abee523de53aef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16341942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burbaeva, Gulnur Sh</creatorcontrib><creatorcontrib>Boksha, Irina S</creatorcontrib><creatorcontrib>Tereshkina, Elena B</creatorcontrib><creatorcontrib>Savushkina, Olga K</creatorcontrib><creatorcontrib>Starodubtseva, Lubov' I</creatorcontrib><creatorcontrib>Turishcheva, Marina S</creatorcontrib><title>Glutamate metabolizing enzymes in prefrontal cortex of Alzheimer's disease patients</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>Amounts of glutamate metabolizing enzymes such as glutamate dehydrogenase (GDH), glutamine synthetase (GS), GS-like protein (GSLP), and phosphate-activated glutaminase (PAG) were compared in prefrontal cortex of control subjects and patients with Alzheimer disease (AD). 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The results may suggest for the pathological interruption of regulatory relations between distinct enzymes of glutamate metabolism in brain of AD patients.</description><subject>Aged</subject><subject>Alzheimer Disease - enzymology</subject><subject>Amide Synthases - metabolism</subject><subject>Female</subject><subject>Glutamate Dehydrogenase - metabolism</subject><subject>Glutamate-Ammonia Ligase - metabolism</subject><subject>Glutamic Acid - metabolism</subject><subject>Glutaminase - metabolism</subject><subject>Humans</subject><subject>Isoenzymes - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Prefrontal Cortex - enzymology</subject><subject>Statistics as Topic</subject><subject>Tissue Extracts - metabolism</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1LAzEQhoMotlZ_gBcJHvS0muwk2d2jFK1CwYN6DtndWd2yXyZZaPvrTWlB8OJpDvO8L8w8hFxydscZS-4d50yJiDEZpUqKaH1EplwmEKmMwTGZMghb4BmbkDPnVoyFVMxPyYQrEDwT8ZS8LZrRm9Z4pC16k_dNva27T4rddtOio3VHB4uV7TtvGlr01uOa9hV9aLZfWLdobx0ta4fGIR2Mr7Hz7pycVKZxeHGYM_Lx9Pg-f46Wr4uX-cMyKkQMPkLJhUpLBixLeGpMLJSSpeBoIM9jrARIVWFZsKTCAovUZCZHlDGUKMFgBTNys-8dbP89ovO6rV2BTWM67EenVZqmAKn4FwyvAKUSFsDrP-CqH20XjtBxzJMk4xICxPdQYXvnwnP0YOvW2I3mTO-86L0XHbzonRe9DpmrQ_GYt1j-Jg4i4Ad8aooH</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Burbaeva, Gulnur Sh</creator><creator>Boksha, Irina S</creator><creator>Tereshkina, Elena B</creator><creator>Savushkina, Olga K</creator><creator>Starodubtseva, Lubov' I</creator><creator>Turishcheva, Marina S</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20051101</creationdate><title>Glutamate metabolizing enzymes in prefrontal cortex of Alzheimer's disease patients</title><author>Burbaeva, Gulnur Sh ; 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| subjects | Aged Alzheimer Disease - enzymology Amide Synthases - metabolism Female Glutamate Dehydrogenase - metabolism Glutamate-Ammonia Ligase - metabolism Glutamic Acid - metabolism Glutaminase - metabolism Humans Isoenzymes - metabolism Male Middle Aged Nerve Tissue Proteins - metabolism Prefrontal Cortex - enzymology Statistics as Topic Tissue Extracts - metabolism |
| title | Glutamate metabolizing enzymes in prefrontal cortex of Alzheimer's disease patients |
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