Knocking out Ubiquitin Proteasome System Function In Vivo and In Vitro with Genetically Encodable Tandem Ubiquitin

At present, the 26S proteasome–specific inhibitor is not available. We constructed polyubiquitin derivatives that contained a tandem repeat of ubiquitins and were insensitive to ubiquitin hydrolases. When these artificial polyubiquitins (tUbs, tandem ubiquitins) were overproduced in the wild‐type ye...

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Veröffentlicht in:Methods in Enzymology 2005, Vol.399, p.64-74
Hauptverfasser: Saeki, Y., Isono, E., Shimada, M., Kawahara, H., Yokosawa, H., Toh‐e, A.
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Sprache:eng
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