Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification
The applicability of cryopreservation protocols to a broad range of genotypes is a key issue for genebanks. We tried to identify the critical factors causing differences in survival of cryopreserved shoot tips using potato varieties coming from cultivated and wild species. The droplet-vitrification...
Gespeichert in:
| Veröffentlicht in: | Cryo-Letters 2006-07, Vol.27 (4), p.223-234 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 234 |
|---|---|
| container_issue | 4 |
| container_start_page | 223 |
| container_title | Cryo-Letters |
| container_volume | 27 |
| creator | Kim, Haeng-Hoon Yoon, Ju-Won Park, Young-Eun Cho, Eun-Gi Sohn, Jae-Keun Kim, Tae-San Engelmann, Florent |
| description | The applicability of cryopreservation protocols to a broad range of genotypes is a key issue for genebanks. We tried to identify the critical factors causing differences in survival of cryopreserved shoot tips using potato varieties coming from cultivated and wild species. The droplet-vitrification
method, a combination of droplet-freezing and solution-based vitrification, was selected from several protocols. High survival after freezing was observed after dehydration with PVS2 for 20 min, cooling shoot tips placed in a droplet of PVS2 solution on aluminum foil strips by immersing the
foil strips in liquid nitrogen, warming them by plunging the foil strips into a 0.8 M sucrose solution (at 40°C) for 30 s and unloading in 0.8 M sucrose for 30 min. This optimized protocol was successfully applied to 12 accessions with survival ranging between 64.0 and 94.4%. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_68880044</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><ingid>cryo/cryo/2006/00000027/00000004/art00004</ingid><sourcerecordid>68880044</sourcerecordid><originalsourceid>FETCH-LOGICAL-i267t-926a0f01eac12da831e9cca033a27885e80e8131b0f2b18abb3a8692b34955ee3</originalsourceid><addsrcrecordid>eNo1kMtOwzAQRbMA0VL4BeQVu0h-JK7Nrqp4SZXYwNqaOBPkksbBdor695i2zGLm6s7RaHQvijlllSg5rapZcR3jllK-lFJfFTMmtaa6pvPiax0OfgwYMewhOT8Q35HRJ0ie2KlPLrvYkj0Eh8lhJDC05Mf1LYkj2mw8EBtcchZ60oFNPkTiBtIGP_aYyN6l4Lq8_Tt9U1x20Ee8Pc9F8fH0-L5-KTdvz6_r1aZ0XC5TqbkE2lGGYBlvQQmG2lqgQgBfKlWjoqiYYA3teMMUNI0AJTVvRKXrGlEsivvT3TH47wljMjsXLfY9DOinaKRSiuZUMnh3Bqdmh60Zg9tBOJj_eDKwOgFu-MQhgdn6KQz5d2NzbKfGKZWGHosvz4JWBkI6CvELsUJ3YQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68880044</pqid></control><display><type>article</type><title>Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification</title><source>MEDLINE</source><source>Ingenta Connect</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Kim, Haeng-Hoon ; Yoon, Ju-Won ; Park, Young-Eun ; Cho, Eun-Gi ; Sohn, Jae-Keun ; Kim, Tae-San ; Engelmann, Florent</creator><creatorcontrib>Kim, Haeng-Hoon ; Yoon, Ju-Won ; Park, Young-Eun ; Cho, Eun-Gi ; Sohn, Jae-Keun ; Kim, Tae-San ; Engelmann, Florent</creatorcontrib><description>The applicability of cryopreservation protocols to a broad range of genotypes is a key issue for genebanks. We tried to identify the critical factors causing differences in survival of cryopreserved shoot tips using potato varieties coming from cultivated and wild species. The droplet-vitrification
method, a combination of droplet-freezing and solution-based vitrification, was selected from several protocols. High survival after freezing was observed after dehydration with PVS2 for 20 min, cooling shoot tips placed in a droplet of PVS2 solution on aluminum foil strips by immersing the
foil strips in liquid nitrogen, warming them by plunging the foil strips into a 0.8 M sucrose solution (at 40°C) for 30 s and unloading in 0.8 M sucrose for 30 min. This optimized protocol was successfully applied to 12 accessions with survival ranging between 64.0 and 94.4%.</description><identifier>ISSN: 0143-2044</identifier><identifier>PMID: 16990950</identifier><language>eng</language><publisher>England: Cryoletters</publisher><subject>Cell Survival - drug effects ; Cell Survival - physiology ; Conservation of Natural Resources ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Culture Media - pharmacology ; Culture Techniques - methods ; Dose-Response Relationship, Drug ; DROPLET-VITRIFICATION ; Genotype ; Glycerol - pharmacology ; Plant Shoots - cytology ; Plant Shoots - drug effects ; Plant Shoots - physiology ; Pvs2/pvs3 Loading ; Shoot Tips ; Solanum - cytology ; Solanum - genetics ; Solanum - physiology ; Solanum Tuberosum L ; Sucrose - pharmacology ; Temperature ; Time Factors</subject><ispartof>Cryo-Letters, 2006-07, Vol.27 (4), p.223-234</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>289,314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16990950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Haeng-Hoon</creatorcontrib><creatorcontrib>Yoon, Ju-Won</creatorcontrib><creatorcontrib>Park, Young-Eun</creatorcontrib><creatorcontrib>Cho, Eun-Gi</creatorcontrib><creatorcontrib>Sohn, Jae-Keun</creatorcontrib><creatorcontrib>Kim, Tae-San</creatorcontrib><creatorcontrib>Engelmann, Florent</creatorcontrib><title>Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification</title><title>Cryo-Letters</title><addtitle>Cryo Letters</addtitle><description>The applicability of cryopreservation protocols to a broad range of genotypes is a key issue for genebanks. We tried to identify the critical factors causing differences in survival of cryopreserved shoot tips using potato varieties coming from cultivated and wild species. The droplet-vitrification
method, a combination of droplet-freezing and solution-based vitrification, was selected from several protocols. High survival after freezing was observed after dehydration with PVS2 for 20 min, cooling shoot tips placed in a droplet of PVS2 solution on aluminum foil strips by immersing the
foil strips in liquid nitrogen, warming them by plunging the foil strips into a 0.8 M sucrose solution (at 40°C) for 30 s and unloading in 0.8 M sucrose for 30 min. This optimized protocol was successfully applied to 12 accessions with survival ranging between 64.0 and 94.4%.</description><subject>Cell Survival - drug effects</subject><subject>Cell Survival - physiology</subject><subject>Conservation of Natural Resources</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Culture Media - pharmacology</subject><subject>Culture Techniques - methods</subject><subject>Dose-Response Relationship, Drug</subject><subject>DROPLET-VITRIFICATION</subject><subject>Genotype</subject><subject>Glycerol - pharmacology</subject><subject>Plant Shoots - cytology</subject><subject>Plant Shoots - drug effects</subject><subject>Plant Shoots - physiology</subject><subject>Pvs2/pvs3 Loading</subject><subject>Shoot Tips</subject><subject>Solanum - cytology</subject><subject>Solanum - genetics</subject><subject>Solanum - physiology</subject><subject>Solanum Tuberosum L</subject><subject>Sucrose - pharmacology</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0143-2044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAQRbMA0VL4BeQVu0h-JK7Nrqp4SZXYwNqaOBPkksbBdor695i2zGLm6s7RaHQvijlllSg5rapZcR3jllK-lFJfFTMmtaa6pvPiax0OfgwYMewhOT8Q35HRJ0ie2KlPLrvYkj0Eh8lhJDC05Mf1LYkj2mw8EBtcchZ60oFNPkTiBtIGP_aYyN6l4Lq8_Tt9U1x20Ee8Pc9F8fH0-L5-KTdvz6_r1aZ0XC5TqbkE2lGGYBlvQQmG2lqgQgBfKlWjoqiYYA3teMMUNI0AJTVvRKXrGlEsivvT3TH47wljMjsXLfY9DOinaKRSiuZUMnh3Bqdmh60Zg9tBOJj_eDKwOgFu-MQhgdn6KQz5d2NzbKfGKZWGHosvz4JWBkI6CvELsUJ3YQ</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Kim, Haeng-Hoon</creator><creator>Yoon, Ju-Won</creator><creator>Park, Young-Eun</creator><creator>Cho, Eun-Gi</creator><creator>Sohn, Jae-Keun</creator><creator>Kim, Tae-San</creator><creator>Engelmann, Florent</creator><general>Cryoletters</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification</title><author>Kim, Haeng-Hoon ; Yoon, Ju-Won ; Park, Young-Eun ; Cho, Eun-Gi ; Sohn, Jae-Keun ; Kim, Tae-San ; Engelmann, Florent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i267t-926a0f01eac12da831e9cca033a27885e80e8131b0f2b18abb3a8692b34955ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Cell Survival - drug effects</topic><topic>Cell Survival - physiology</topic><topic>Conservation of Natural Resources</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Culture Media - pharmacology</topic><topic>Culture Techniques - methods</topic><topic>Dose-Response Relationship, Drug</topic><topic>DROPLET-VITRIFICATION</topic><topic>Genotype</topic><topic>Glycerol - pharmacology</topic><topic>Plant Shoots - cytology</topic><topic>Plant Shoots - drug effects</topic><topic>Plant Shoots - physiology</topic><topic>Pvs2/pvs3 Loading</topic><topic>Shoot Tips</topic><topic>Solanum - cytology</topic><topic>Solanum - genetics</topic><topic>Solanum - physiology</topic><topic>Solanum Tuberosum L</topic><topic>Sucrose - pharmacology</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Haeng-Hoon</creatorcontrib><creatorcontrib>Yoon, Ju-Won</creatorcontrib><creatorcontrib>Park, Young-Eun</creatorcontrib><creatorcontrib>Cho, Eun-Gi</creatorcontrib><creatorcontrib>Sohn, Jae-Keun</creatorcontrib><creatorcontrib>Kim, Tae-San</creatorcontrib><creatorcontrib>Engelmann, Florent</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cryo-Letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Haeng-Hoon</au><au>Yoon, Ju-Won</au><au>Park, Young-Eun</au><au>Cho, Eun-Gi</au><au>Sohn, Jae-Keun</au><au>Kim, Tae-San</au><au>Engelmann, Florent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification</atitle><jtitle>Cryo-Letters</jtitle><addtitle>Cryo Letters</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>27</volume><issue>4</issue><spage>223</spage><epage>234</epage><pages>223-234</pages><issn>0143-2044</issn><abstract>The applicability of cryopreservation protocols to a broad range of genotypes is a key issue for genebanks. We tried to identify the critical factors causing differences in survival of cryopreserved shoot tips using potato varieties coming from cultivated and wild species. The droplet-vitrification
method, a combination of droplet-freezing and solution-based vitrification, was selected from several protocols. High survival after freezing was observed after dehydration with PVS2 for 20 min, cooling shoot tips placed in a droplet of PVS2 solution on aluminum foil strips by immersing the
foil strips in liquid nitrogen, warming them by plunging the foil strips into a 0.8 M sucrose solution (at 40°C) for 30 s and unloading in 0.8 M sucrose for 30 min. This optimized protocol was successfully applied to 12 accessions with survival ranging between 64.0 and 94.4%.</abstract><cop>England</cop><pub>Cryoletters</pub><pmid>16990950</pmid><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0143-2044 |
| ispartof | Cryo-Letters, 2006-07, Vol.27 (4), p.223-234 |
| issn | 0143-2044 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68880044 |
| source | MEDLINE; Ingenta Connect; EZB-FREE-00999 freely available EZB journals |
| subjects | Cell Survival - drug effects Cell Survival - physiology Conservation of Natural Resources Cryopreservation - methods Cryoprotective Agents - pharmacology Culture Media - pharmacology Culture Techniques - methods Dose-Response Relationship, Drug DROPLET-VITRIFICATION Genotype Glycerol - pharmacology Plant Shoots - cytology Plant Shoots - drug effects Plant Shoots - physiology Pvs2/pvs3 Loading Shoot Tips Solanum - cytology Solanum - genetics Solanum - physiology Solanum Tuberosum L Sucrose - pharmacology Temperature Time Factors |
| title | Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T09%3A40%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cryopreservation%20of%20potato%20cultivated%20varieties%20and%20wild%20species:%20critical%20factors%20in%20droplet%20vitrification&rft.jtitle=Cryo-Letters&rft.au=Kim,%20Haeng-Hoon&rft.date=2006-07-01&rft.volume=27&rft.issue=4&rft.spage=223&rft.epage=234&rft.pages=223-234&rft.issn=0143-2044&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E68880044%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68880044&rft_id=info:pmid/16990950&rft_ingid=cryo/cryo/2006/00000027/00000004/art00004&rfr_iscdi=true |