Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements
Our understanding of the mechanism of protein synthesis has undergone rapid progress in recent years as a result of low-resolution X-ray and cryo-EM structures of ribosome functional complexes and high-resolution structures of ribosomal subunits and vacant ribosomes. Here, we present the crystal str...
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| Veröffentlicht in: | Cell 2006-09, Vol.126 (6), p.1065-1077 |
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| container_title | Cell |
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| creator | Korostelev, Andrei Trakhanov, Sergei Laurberg, Martin Noller, Harry F. |
| description | Our understanding of the mechanism of protein synthesis has undergone rapid progress in recent years as a result of low-resolution X-ray and cryo-EM structures of ribosome functional complexes and high-resolution structures of ribosomal subunits and vacant ribosomes. Here, we present the crystal structure of the
Thermus thermophilus 70S ribosome containing a model mRNA and two tRNAs at 3.7 Å resolution. Many structural details of the interactions between the ribosome, tRNA, and mRNA in the P and E sites and the ways in which tRNA structure is distorted by its interactions with the ribosome are seen. Differences between the conformations of vacant and tRNA-bound 70S ribosomes suggest an induced fit of the ribosome structure in response to tRNA binding, including significant changes in the peptidyl-transferase catalytic site. |
| doi_str_mv | 10.1016/j.cell.2006.08.032 |
| format | Article |
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Differences between the conformations of vacant and tRNA-bound 70S ribosomes suggest an induced fit of the ribosome structure in response to tRNA binding, including significant changes in the peptidyl-transferase catalytic site.</description><subject>Bacterial Proteins - biosynthesis</subject><subject>Crystallography, X-Ray</subject><subject>Macromolecular Substances - chemistry</subject><subject>Nucleic Acid Conformation</subject><subject>Ribosomes - chemistry</subject><subject>Ribosomes - genetics</subject><subject>RNA, Messenger - chemistry</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Ribosomal - chemistry</subject><subject>RNA, Ribosomal - genetics</subject><subject>RNA, Transfer - chemistry</subject><subject>RNA, Transfer - genetics</subject><subject>Thermus thermophilus</subject><subject>Thermus thermophilus - genetics</subject><subject>Thermus thermophilus - metabolism</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo7jj6BTxITt66rSSddAJelmFXFxaFWT2HdFItPfSfMUkv7rc34wx400sVBe_3KN4j5C2DmgFTHw61x3GsOYCqQdcg-DOyYWDaqmEtf042AIZXWrXNFXmV0gEAtJTyJbliyiiuZLMhfhefUnYjfchx9XmNSJeeOtrCA90P3ZKWCau8_3JNd8t0HPEX3eMjujHR23X2eVjmwt7NGaP7cyXq5lA0LkY3_8AJ55xekxd9IfDNZW_J99ubb7vP1f3XT3e76_vKN0bmqm19J5kWhnflP-OCDwZEz3Toeg-qC50SspdNed0F6WWQouna3juhQXMjxJa8P_se4_JzxZTtNKRTRG7GZU1Wad0ao_R_hcwI3sgyt4SfhT4uKUXs7TEOk4tPloE9dWAP9sTZUwcWtC0dFOjdxX3tJgx_kUvoRfDxLMASxuOA0SY_4OwxDBF9tmEZ_uX_GzTzmDs</recordid><startdate>20060922</startdate><enddate>20060922</enddate><creator>Korostelev, Andrei</creator><creator>Trakhanov, Sergei</creator><creator>Laurberg, Martin</creator><creator>Noller, Harry F.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20060922</creationdate><title>Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements</title><author>Korostelev, Andrei ; 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| ispartof | Cell, 2006-09, Vol.126 (6), p.1065-1077 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; EZB-FREE-00999 freely available EZB journals |
| subjects | Bacterial Proteins - biosynthesis Crystallography, X-Ray Macromolecular Substances - chemistry Nucleic Acid Conformation Ribosomes - chemistry Ribosomes - genetics RNA, Messenger - chemistry RNA, Messenger - genetics RNA, Ribosomal - chemistry RNA, Ribosomal - genetics RNA, Transfer - chemistry RNA, Transfer - genetics Thermus thermophilus Thermus thermophilus - genetics Thermus thermophilus - metabolism |
| title | Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements |
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