Purification of transgenic plant-derived recombinant human acetylcholinesterase-R

Nicotiana benthamiana plants were engineered to express a codon-optimized gene encoding the human acetylcholinesterase-R (AChE) isoform. The transgenic plants expressed the protein at >0.4% of total soluble protein, and the plant-produced enzyme was purified to homogeneity. Following lysis, proca...

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Veröffentlicht in:	Chemico-biological interactions 2005-12, Vol.157, p.331-334
Hauptverfasser:	Geyer, Brian C., Muralidharan, Mrinalini, Cherni, Irene, Doran, Jeffrey, Fletcher, Samuel P., Evron, Tama, Soreq, Hermona, Mor, Tsafrir S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcholinesterase Acetylcholinesterase - biosynthesis Acetylcholinesterase - genetics Acetylcholinesterase - isolation & purification Acetylcholinesterase - metabolism Cell Line Electrophoresis, Polyacrylamide Gel Humans Kinetics Molecular pharming Nicotiana - genetics Plants, Genetically Modified Protein purification Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Tobacco Mosaic Virus - genetics Transgenic plants
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