Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol
Contents This study was conducted to examine whether cat spermatozoa stored in ethanol for 1 month was capable of developing into pronuclei and of supporting normal embryonic development. In vitro matured oocytes were injected with frozen–thawed spermatozoa and ethanol‐stored spermatozoa. The status...
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| Veröffentlicht in: | Reproduction in domestic animals 2005-12, Vol.40 (6), p.511-515 |
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| container_title | Reproduction in domestic animals |
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| creator | Murakami, M Karja, N.W.K Wongsrikeao, P Agung, B Taniguchi, M Naoi, H Otoi, T |
| description | Contents
This study was conducted to examine whether cat spermatozoa stored in ethanol for 1 month was capable of developing into pronuclei and of supporting normal embryonic development. In vitro matured oocytes were injected with frozen–thawed spermatozoa and ethanol‐stored spermatozoa. The status of oocytes and sperm nuclei was examined at 4 and 18 h after injection of spermatozoa, and the presumptive zygotes were cultured for 7 days to assess the development of oocytes injected with each storage sperm. The percentage of enlarged sperm head at 4 h after injection was higher in ethanol‐stored spermatozoa than in frozen–thawed spermatozoa, but there was no significant difference in the development of oocytes and sperm nuclei at 18 h after injection between the two groups. The development of oocytes to the blastocyst stage after injection of spermatozoa was observed only in oocytes with frozen–thawed spermatozoa. Of oocytes injected with ethanol‐stored spermatozoa, two (2.8%) oocytes developed to the 16‐cell stage. These results indicate that cat spermatozoa stored in ethanol can decondense and form male pronuclei after intracytoplasmic injection. However, the oocytes injected with ethanol‐stored spermatozoa did not have the ability to develop to the blastocyst stage. |
| doi_str_mv | 10.1111/j.1439-0531.2005.00617.x |
| format | Article |
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This study was conducted to examine whether cat spermatozoa stored in ethanol for 1 month was capable of developing into pronuclei and of supporting normal embryonic development. In vitro matured oocytes were injected with frozen–thawed spermatozoa and ethanol‐stored spermatozoa. The status of oocytes and sperm nuclei was examined at 4 and 18 h after injection of spermatozoa, and the presumptive zygotes were cultured for 7 days to assess the development of oocytes injected with each storage sperm. The percentage of enlarged sperm head at 4 h after injection was higher in ethanol‐stored spermatozoa than in frozen–thawed spermatozoa, but there was no significant difference in the development of oocytes and sperm nuclei at 18 h after injection between the two groups. The development of oocytes to the blastocyst stage after injection of spermatozoa was observed only in oocytes with frozen–thawed spermatozoa. Of oocytes injected with ethanol‐stored spermatozoa, two (2.8%) oocytes developed to the 16‐cell stage. These results indicate that cat spermatozoa stored in ethanol can decondense and form male pronuclei after intracytoplasmic injection. However, the oocytes injected with ethanol‐stored spermatozoa did not have the ability to develop to the blastocyst stage.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2005.00617.x</identifier><identifier>PMID: 16324075</identifier><language>eng</language><publisher>Berlin, Germany: Blackwell Verlag GmbH</publisher><subject>Animals ; artificial insemination ; Biological and medical sciences ; Blastocyst - physiology ; Breeding of animals ; Cats ; Cats - embryology ; Cats - physiology ; Cell Nucleus - drug effects ; Cell Nucleus - physiology ; Cells, Cultured ; embryo (animal) ; embryogenesis ; Embryos ; Ethanol ; Ethanol - pharmacology ; Female ; frozen storage ; Fundamental and applied biological sciences. Psychology ; Male ; Mammalian reproduction. General aspects ; oocytes ; Oocytes - drug effects ; Oocytes - physiology ; Pregnancy ; Reproductive technologies ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sperm Injections, Intracytoplasmic - methods ; Sperm Injections, Intracytoplasmic - veterinary ; spermatozoa ; Spermatozoa - physiology ; storage time ; Vertebrates: reproduction</subject><ispartof>Reproduction in domestic animals, 2005-12, Vol.40 (6), p.511-515</ispartof><rights>2006 INIST-CNRS</rights><rights>2005 Blackwell Verlag, Berlin</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5397-b23d7bf4ad2c5f2f141dbe161df7f7d15d92f7110f67e642de8bba3549995b183</citedby><cites>FETCH-LOGICAL-c5397-b23d7bf4ad2c5f2f141dbe161df7f7d15d92f7110f67e642de8bba3549995b183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2005.00617.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2005.00617.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17290674$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16324075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murakami, M</creatorcontrib><creatorcontrib>Karja, N.W.K</creatorcontrib><creatorcontrib>Wongsrikeao, P</creatorcontrib><creatorcontrib>Agung, B</creatorcontrib><creatorcontrib>Taniguchi, M</creatorcontrib><creatorcontrib>Naoi, H</creatorcontrib><creatorcontrib>Otoi, T</creatorcontrib><title>Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
This study was conducted to examine whether cat spermatozoa stored in ethanol for 1 month was capable of developing into pronuclei and of supporting normal embryonic development. In vitro matured oocytes were injected with frozen–thawed spermatozoa and ethanol‐stored spermatozoa. The status of oocytes and sperm nuclei was examined at 4 and 18 h after injection of spermatozoa, and the presumptive zygotes were cultured for 7 days to assess the development of oocytes injected with each storage sperm. The percentage of enlarged sperm head at 4 h after injection was higher in ethanol‐stored spermatozoa than in frozen–thawed spermatozoa, but there was no significant difference in the development of oocytes and sperm nuclei at 18 h after injection between the two groups. The development of oocytes to the blastocyst stage after injection of spermatozoa was observed only in oocytes with frozen–thawed spermatozoa. Of oocytes injected with ethanol‐stored spermatozoa, two (2.8%) oocytes developed to the 16‐cell stage. These results indicate that cat spermatozoa stored in ethanol can decondense and form male pronuclei after intracytoplasmic injection. However, the oocytes injected with ethanol‐stored spermatozoa did not have the ability to develop to the blastocyst stage.</description><subject>Animals</subject><subject>artificial insemination</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - physiology</subject><subject>Breeding of animals</subject><subject>Cats</subject><subject>Cats - embryology</subject><subject>Cats - physiology</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - physiology</subject><subject>Cells, Cultured</subject><subject>embryo (animal)</subject><subject>embryogenesis</subject><subject>Embryos</subject><subject>Ethanol</subject><subject>Ethanol - pharmacology</subject><subject>Female</subject><subject>frozen storage</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Mammalian reproduction. General aspects</subject><subject>oocytes</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Pregnancy</subject><subject>Reproductive technologies</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Sperm Injections, Intracytoplasmic - methods</subject><subject>Sperm Injections, Intracytoplasmic - veterinary</subject><subject>spermatozoa</subject><subject>Spermatozoa - physiology</subject><subject>storage time</subject><subject>Vertebrates: reproduction</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV9r1jAUh4s43Ov0K2gR9K41J39b8GZsbg6HgnPsMqRpon1tmy5p9a2f3tS-bODNzE1C8jwnh_NLkhRQDnG93eZASZkhRiDHCLEcIQ4i3z1KNncPj5MNKgnPuODFYfI0hC1CwAohniSHwAmmSLBNUp2an6Z1Q2f6MXU21WpMTVf52YV08K6etKnTak6bfvRKz6MbWhW6RseLrdFj4_rFCoPxnRrdb6fSMDofnaZPVavdd9c-Sw6saoN5vt-Pkuuz919PPmSXn88vTo4vM81IKbIKk1pUlqoaa2axBQp1ZYBDbYUVNbC6xFYAIMuF4RTXpqgqRRgty5JVUJCj5M1aN_Z9O5kwyq4J2rSt6o2bguRFIRglJIKv_gG3bvJ97E1iIIKKOLaHoYLBwxBny3fFCmnvQvDGysE3nfKzBCSXOOVWLqnJJTW5xCn_xil3UX2xrz9VnanvxX1-EXi9B1TQqrVe9boJ95zAJeKCRu7dyv1qWjP_dwPyy-lxPEQ9W_UmjGZ3pyv_Q3JBBJM3n87lR3olQNwweRb5lytvlZPqm48tXV9hBAQBYnF0BfkDgyvSNw</recordid><startdate>200512</startdate><enddate>200512</enddate><creator>Murakami, M</creator><creator>Karja, N.W.K</creator><creator>Wongsrikeao, P</creator><creator>Agung, B</creator><creator>Taniguchi, M</creator><creator>Naoi, H</creator><creator>Otoi, T</creator><general>Blackwell Verlag GmbH</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200512</creationdate><title>Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol</title><author>Murakami, M ; Karja, N.W.K ; Wongsrikeao, P ; Agung, B ; Taniguchi, M ; Naoi, H ; Otoi, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5397-b23d7bf4ad2c5f2f141dbe161df7f7d15d92f7110f67e642de8bba3549995b183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>artificial insemination</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - physiology</topic><topic>Breeding of animals</topic><topic>Cats</topic><topic>Cats - embryology</topic><topic>Cats - physiology</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - physiology</topic><topic>Cells, Cultured</topic><topic>embryo (animal)</topic><topic>embryogenesis</topic><topic>Embryos</topic><topic>Ethanol</topic><topic>Ethanol - pharmacology</topic><topic>Female</topic><topic>frozen storage</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Mammalian reproduction. General aspects</topic><topic>oocytes</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Pregnancy</topic><topic>Reproductive technologies</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Sperm Injections, Intracytoplasmic - methods</topic><topic>Sperm Injections, Intracytoplasmic - veterinary</topic><topic>spermatozoa</topic><topic>Spermatozoa - physiology</topic><topic>storage time</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murakami, M</creatorcontrib><creatorcontrib>Karja, N.W.K</creatorcontrib><creatorcontrib>Wongsrikeao, P</creatorcontrib><creatorcontrib>Agung, B</creatorcontrib><creatorcontrib>Taniguchi, M</creatorcontrib><creatorcontrib>Naoi, H</creatorcontrib><creatorcontrib>Otoi, T</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murakami, M</au><au>Karja, N.W.K</au><au>Wongsrikeao, P</au><au>Agung, B</au><au>Taniguchi, M</au><au>Naoi, H</au><au>Otoi, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2005-12</date><risdate>2005</risdate><volume>40</volume><issue>6</issue><spage>511</spage><epage>515</epage><pages>511-515</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
This study was conducted to examine whether cat spermatozoa stored in ethanol for 1 month was capable of developing into pronuclei and of supporting normal embryonic development. In vitro matured oocytes were injected with frozen–thawed spermatozoa and ethanol‐stored spermatozoa. The status of oocytes and sperm nuclei was examined at 4 and 18 h after injection of spermatozoa, and the presumptive zygotes were cultured for 7 days to assess the development of oocytes injected with each storage sperm. The percentage of enlarged sperm head at 4 h after injection was higher in ethanol‐stored spermatozoa than in frozen–thawed spermatozoa, but there was no significant difference in the development of oocytes and sperm nuclei at 18 h after injection between the two groups. The development of oocytes to the blastocyst stage after injection of spermatozoa was observed only in oocytes with frozen–thawed spermatozoa. Of oocytes injected with ethanol‐stored spermatozoa, two (2.8%) oocytes developed to the 16‐cell stage. These results indicate that cat spermatozoa stored in ethanol can decondense and form male pronuclei after intracytoplasmic injection. However, the oocytes injected with ethanol‐stored spermatozoa did not have the ability to develop to the blastocyst stage.</abstract><cop>Berlin, Germany</cop><pub>Blackwell Verlag GmbH</pub><pmid>16324075</pmid><doi>10.1111/j.1439-0531.2005.00617.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Reproduction in domestic animals, 2005-12, Vol.40 (6), p.511-515 |
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| subjects | Animals artificial insemination Biological and medical sciences Blastocyst - physiology Breeding of animals Cats Cats - embryology Cats - physiology Cell Nucleus - drug effects Cell Nucleus - physiology Cells, Cultured embryo (animal) embryogenesis Embryos Ethanol Ethanol - pharmacology Female frozen storage Fundamental and applied biological sciences. Psychology Male Mammalian reproduction. General aspects oocytes Oocytes - drug effects Oocytes - physiology Pregnancy Reproductive technologies Semen Preservation - methods Semen Preservation - veterinary Sperm Injections, Intracytoplasmic - methods Sperm Injections, Intracytoplasmic - veterinary spermatozoa Spermatozoa - physiology storage time Vertebrates: reproduction |
| title | Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol |
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