Real-time RT-PCR: A Complementary Method to Detect HER-2 Status in Breast Carcinoma

The option to treat patients presenting with HER-2 overexpressed invasive breast carcinoma with HerceptinÂ® requires quantitative determination of the HER-2 status. The aim of this study was to retrospectively evaluate the HER-2 mRNA expression levels using quantitative real-time RT-PCR (Q-RT-PCR) i...

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Veröffentlicht in:	Anticancer research 2005-11, Vol.25 (6C), p.4679-4683
Hauptverfasser:	BOSSARD, Céline, BIECHE, Ivan, LE DOUSSAL, Viviane, LIDEREAU, Rosette, SABOURIN, Jean Christophe
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Aged, 80 and over Biological and medical sciences Breast Neoplasms - genetics Breast Neoplasms - metabolism Female Gynecology. Andrology. Obstetrics Humans Mammary gland diseases Medical sciences Middle Aged Receptor, ErbB-2 - biosynthesis Receptor, ErbB-2 - genetics Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - biosynthesis RNA, Messenger - genetics Taq Polymerase - metabolism Tumors
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Zusammenfassung:	The option to treat patients presenting with HER-2 overexpressed invasive breast carcinoma with HerceptinÂ® requires quantitative determination of the HER-2 status. The aim of this study was to retrospectively evaluate the HER-2 mRNA expression levels using quantitative real-time RT-PCR (Q-RT-PCR) in tissue samples from 44 primary breast carcinomas and compare the results with immunohistochemistry (IHC). To determine the cut-off for altered mRNA expression, a normalized HER-2 expression value was determined for 20 normal breast tissue RNAs. Gene expression was categorized into three groups: normal expression (mRNAs
ISSN:	0250-7005 1791-7530