Promoter methylation in circadian genes of endometrial cancers detected by methylation-specific PCR
Methylation of CpG dinucleotides in the promoter sequence of a gene can lead to deregulated and suppressed gene expression. In this study, we have developed procedures for methylation‐specific polymerase chain reaction (MSP) and sequencing analysis to determine CpG methylation status of the promoter...
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| Veröffentlicht in: | Molecular carcinogenesis 2006-10, Vol.45 (10), p.732-740 |
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| creator | Shih, Mu-Chin Yeh, Kun-Tu Tang, Kai-Ping Chen, Jui-Chang Chang, Jan-Gowth |
| description | Methylation of CpG dinucleotides in the promoter sequence of a gene can lead to deregulated and suppressed gene expression. In this study, we have developed procedures for methylation‐specific polymerase chain reaction (MSP) and sequencing analysis to determine CpG methylation status of the promoter sequences of nine circadian genes in 35 endometrial cancers (EC) and paired noncancerous endometrial tissues. DNA methylation was found in the promoter sequences of PER1, PER2, and CRY1, but not of other six circadian genes in the ECs and normal tissues examined. Eleven of the 35 EC tissues showed CpG methylation in the promoter sequences of PER1, PER2, or CRY1. Of these 11 cases, 1 had promoter methylation in all the three genes, 1 in PER1 and PER2, 3 in PER1 and CRY1, and 6 in PER1, respectively. In comparison, promoter CpG methylation of PER1, PER2, or CRY1 was found in only 7 of 35 paired noncancerous tissues including 2 in PER1 and PER2, 2 in PER1, and 3 in CRY1. In summary, promoter methylation in the PER1, PER2, or CRY1 circadian genes was detected in about one‐third of EC and one‐fifth of noncancerous endometrial tissues of 35 paired specimens indicating possible disruption of the circadian clock in the development of EC. © 2006 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/mc.20198 |
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In this study, we have developed procedures for methylation‐specific polymerase chain reaction (MSP) and sequencing analysis to determine CpG methylation status of the promoter sequences of nine circadian genes in 35 endometrial cancers (EC) and paired noncancerous endometrial tissues. DNA methylation was found in the promoter sequences of PER1, PER2, and CRY1, but not of other six circadian genes in the ECs and normal tissues examined. Eleven of the 35 EC tissues showed CpG methylation in the promoter sequences of PER1, PER2, or CRY1. Of these 11 cases, 1 had promoter methylation in all the three genes, 1 in PER1 and PER2, 3 in PER1 and CRY1, and 6 in PER1, respectively. In comparison, promoter CpG methylation of PER1, PER2, or CRY1 was found in only 7 of 35 paired noncancerous tissues including 2 in PER1 and PER2, 2 in PER1, and 3 in CRY1. In summary, promoter methylation in the PER1, PER2, or CRY1 circadian genes was detected in about one‐third of EC and one‐fifth of noncancerous endometrial tissues of 35 paired specimens indicating possible disruption of the circadian clock in the development of EC. © 2006 Wiley‐Liss, Inc.</description><identifier>ISSN: 0899-1987</identifier><identifier>EISSN: 1098-2744</identifier><identifier>DOI: 10.1002/mc.20198</identifier><identifier>PMID: 16683245</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Aged ; ARNTL Transcription Factors ; Base Sequence ; Basic Helix-Loop-Helix Transcription Factors - genetics ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; circadian genes ; Circadian Rhythm - genetics ; CLOCK Proteins ; DNA Methylation ; endometrial cancer ; Endometrial Neoplasms - genetics ; Endometrial Neoplasms - metabolism ; Endometrial Neoplasms - pathology ; Female ; Flavoproteins - genetics ; Flavoproteins - metabolism ; Guanine Nucleotide Exchange Factors ; Humans ; Immunohistochemistry ; methylation-specific PCR ; Middle Aged ; Molecular Sequence Data ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Polymerase Chain Reaction - methods ; promoter ; Promoter Regions, Genetic - genetics ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; Rho Guanine Nucleotide Exchange Factors ; Trans-Activators - genetics ; Trans-Activators - metabolism</subject><ispartof>Molecular carcinogenesis, 2006-10, Vol.45 (10), p.732-740</ispartof><rights>Copyright © 2006 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3888-9c846ff782a592d2dbd154507ef458a41c376760edd3a5796b24778af4654503</citedby><cites>FETCH-LOGICAL-c3888-9c846ff782a592d2dbd154507ef458a41c376760edd3a5796b24778af4654503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmc.20198$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmc.20198$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16683245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shih, Mu-Chin</creatorcontrib><creatorcontrib>Yeh, Kun-Tu</creatorcontrib><creatorcontrib>Tang, Kai-Ping</creatorcontrib><creatorcontrib>Chen, Jui-Chang</creatorcontrib><creatorcontrib>Chang, Jan-Gowth</creatorcontrib><title>Promoter methylation in circadian genes of endometrial cancers detected by methylation-specific PCR</title><title>Molecular carcinogenesis</title><addtitle>Mol. Carcinog</addtitle><description>Methylation of CpG dinucleotides in the promoter sequence of a gene can lead to deregulated and suppressed gene expression. In this study, we have developed procedures for methylation‐specific polymerase chain reaction (MSP) and sequencing analysis to determine CpG methylation status of the promoter sequences of nine circadian genes in 35 endometrial cancers (EC) and paired noncancerous endometrial tissues. DNA methylation was found in the promoter sequences of PER1, PER2, and CRY1, but not of other six circadian genes in the ECs and normal tissues examined. Eleven of the 35 EC tissues showed CpG methylation in the promoter sequences of PER1, PER2, or CRY1. Of these 11 cases, 1 had promoter methylation in all the three genes, 1 in PER1 and PER2, 3 in PER1 and CRY1, and 6 in PER1, respectively. In comparison, promoter CpG methylation of PER1, PER2, or CRY1 was found in only 7 of 35 paired noncancerous tissues including 2 in PER1 and PER2, 2 in PER1, and 3 in CRY1. In summary, promoter methylation in the PER1, PER2, or CRY1 circadian genes was detected in about one‐third of EC and one‐fifth of noncancerous endometrial tissues of 35 paired specimens indicating possible disruption of the circadian clock in the development of EC. © 2006 Wiley‐Liss, Inc.</description><subject>Adult</subject><subject>Aged</subject><subject>ARNTL Transcription Factors</subject><subject>Base Sequence</subject><subject>Basic Helix-Loop-Helix Transcription Factors - genetics</subject><subject>Basic Helix-Loop-Helix Transcription Factors - metabolism</subject><subject>circadian genes</subject><subject>Circadian Rhythm - genetics</subject><subject>CLOCK Proteins</subject><subject>DNA Methylation</subject><subject>endometrial cancer</subject><subject>Endometrial Neoplasms - genetics</subject><subject>Endometrial Neoplasms - metabolism</subject><subject>Endometrial Neoplasms - pathology</subject><subject>Female</subject><subject>Flavoproteins - genetics</subject><subject>Flavoproteins - metabolism</subject><subject>Guanine Nucleotide Exchange Factors</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>methylation-specific PCR</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Polymerase Chain Reaction - methods</subject><subject>promoter</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Rho Guanine Nucleotide Exchange Factors</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><issn>0899-1987</issn><issn>1098-2744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EtLAzEUhuEgitYL-AskK3Ezmkyus5RiVfCO6DKkyRmNzqUmU7T_3tHWy0ZcHTg8vIsPoW1K9ikh-UHt9nNCC72EBpQUOssV58toQHRRZP1braH1lJ4IoVQJsorWqJSa5VwMkLuKbd12EHEN3eOssl1oGxwa7EJ01gfb4AdoIOG2xND4tlcx2Ao72ziICXvowHXg8Xj2u5ClCbhQBoevhjebaKW0VYKtxd1At6Oj2-FJdnZ5fDo8PMsc01pnhdNclqXSuRVF7nM_9lRwQRSUXGjLqWNKKknAe2aFKuQ450ppW3L5wdgG2p1nJ7F9mULqTB2Sg6qyDbTTZKTWihDC_4X9Yr2VrId7c-him1KE0kxiqG2cGUrMx_CmduZz-J7uLJrTcQ3-By6W7kE2B6-hgtmfIXM-_AoufEgdvH17G5-NVEwJc39xbK7ZqLi7F-eGsHdet5rB</recordid><startdate>200610</startdate><enddate>200610</enddate><creator>Shih, Mu-Chin</creator><creator>Yeh, Kun-Tu</creator><creator>Tang, Kai-Ping</creator><creator>Chen, Jui-Chang</creator><creator>Chang, Jan-Gowth</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200610</creationdate><title>Promoter methylation in circadian genes of endometrial cancers detected by methylation-specific PCR</title><author>Shih, Mu-Chin ; Yeh, Kun-Tu ; Tang, Kai-Ping ; Chen, Jui-Chang ; Chang, Jan-Gowth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3888-9c846ff782a592d2dbd154507ef458a41c376760edd3a5796b24778af4654503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Aged</topic><topic>ARNTL Transcription Factors</topic><topic>Base Sequence</topic><topic>Basic Helix-Loop-Helix Transcription Factors - genetics</topic><topic>Basic Helix-Loop-Helix Transcription Factors - metabolism</topic><topic>circadian genes</topic><topic>Circadian Rhythm - genetics</topic><topic>CLOCK Proteins</topic><topic>DNA Methylation</topic><topic>endometrial cancer</topic><topic>Endometrial Neoplasms - genetics</topic><topic>Endometrial Neoplasms - metabolism</topic><topic>Endometrial Neoplasms - pathology</topic><topic>Female</topic><topic>Flavoproteins - genetics</topic><topic>Flavoproteins - metabolism</topic><topic>Guanine Nucleotide Exchange Factors</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>methylation-specific PCR</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Polymerase Chain Reaction - methods</topic><topic>promoter</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Rho Guanine Nucleotide Exchange Factors</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shih, Mu-Chin</creatorcontrib><creatorcontrib>Yeh, Kun-Tu</creatorcontrib><creatorcontrib>Tang, Kai-Ping</creatorcontrib><creatorcontrib>Chen, Jui-Chang</creatorcontrib><creatorcontrib>Chang, Jan-Gowth</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular carcinogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shih, Mu-Chin</au><au>Yeh, Kun-Tu</au><au>Tang, Kai-Ping</au><au>Chen, Jui-Chang</au><au>Chang, Jan-Gowth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promoter methylation in circadian genes of endometrial cancers detected by methylation-specific PCR</atitle><jtitle>Molecular carcinogenesis</jtitle><addtitle>Mol. Carcinog</addtitle><date>2006-10</date><risdate>2006</risdate><volume>45</volume><issue>10</issue><spage>732</spage><epage>740</epage><pages>732-740</pages><issn>0899-1987</issn><eissn>1098-2744</eissn><abstract>Methylation of CpG dinucleotides in the promoter sequence of a gene can lead to deregulated and suppressed gene expression. In this study, we have developed procedures for methylation‐specific polymerase chain reaction (MSP) and sequencing analysis to determine CpG methylation status of the promoter sequences of nine circadian genes in 35 endometrial cancers (EC) and paired noncancerous endometrial tissues. DNA methylation was found in the promoter sequences of PER1, PER2, and CRY1, but not of other six circadian genes in the ECs and normal tissues examined. Eleven of the 35 EC tissues showed CpG methylation in the promoter sequences of PER1, PER2, or CRY1. Of these 11 cases, 1 had promoter methylation in all the three genes, 1 in PER1 and PER2, 3 in PER1 and CRY1, and 6 in PER1, respectively. In comparison, promoter CpG methylation of PER1, PER2, or CRY1 was found in only 7 of 35 paired noncancerous tissues including 2 in PER1 and PER2, 2 in PER1, and 3 in CRY1. In summary, promoter methylation in the PER1, PER2, or CRY1 circadian genes was detected in about one‐third of EC and one‐fifth of noncancerous endometrial tissues of 35 paired specimens indicating possible disruption of the circadian clock in the development of EC. © 2006 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16683245</pmid><doi>10.1002/mc.20198</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult Aged ARNTL Transcription Factors Base Sequence Basic Helix-Loop-Helix Transcription Factors - genetics Basic Helix-Loop-Helix Transcription Factors - metabolism circadian genes Circadian Rhythm - genetics CLOCK Proteins DNA Methylation endometrial cancer Endometrial Neoplasms - genetics Endometrial Neoplasms - metabolism Endometrial Neoplasms - pathology Female Flavoproteins - genetics Flavoproteins - metabolism Guanine Nucleotide Exchange Factors Humans Immunohistochemistry methylation-specific PCR Middle Aged Molecular Sequence Data Nuclear Proteins - genetics Nuclear Proteins - metabolism Polymerase Chain Reaction - methods promoter Promoter Regions, Genetic - genetics Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Rho Guanine Nucleotide Exchange Factors Trans-Activators - genetics Trans-Activators - metabolism |
| title | Promoter methylation in circadian genes of endometrial cancers detected by methylation-specific PCR |
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