Comparison of HPLC method and commercial ELISA assay for asymmetric dimethylarginine (ADMA) determination in human serum
The performance of a new ELISA assay kit (DLD Diagnostika GmbH, Hamburg, Germany) for the determination of asymmetric dimethylarginine (ADMA) was evaluated against a reversed phase HPLC method. ADMA concentrations of 55 serum samples were measured with both methods. The intra-assay CV for ADMA-ELISA...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-12, Vol.828 (1), p.97-102 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Valtonen, Pirjo Karppi, Jouni Nyyssönen, Kristiina Valkonen, Veli-Pekka Halonen, Toivo Punnonen, Kari |
| description | The performance of a new ELISA assay kit (DLD Diagnostika GmbH, Hamburg, Germany) for the determination of asymmetric dimethylarginine (ADMA) was evaluated against a reversed phase HPLC method. ADMA concentrations of 55 serum samples were measured with both methods. The intra-assay CV for ADMA-ELISA was 19% (
n
=
10). Inter-assay CVs for ADMA-ELISA were 9% for kit control 1 (0.410
±
0.037
μM) and 14% for kit control 2 (1.174
±
0.165
μM). The intra- and inter-assay CVs for HPLC assay for ADMA were 2.5% (0.586
±
0.015
μM) and 4.2% (0.664
±
0.028
μM), respectively. There was no correlation between these two methods (
R
2
=
0.0972). The effect of storage conditions of the samples on ADMA concentrations was investigated by HPLC. ADMA concentration was stable after four freezing and thawing cycles. Overall, the HPLC method offered better sensitivity, selectivity and, very importantly, simultaneous determination of ADMA, SDMA,
l-homoarginine and
l-arginine. |
| doi_str_mv | 10.1016/j.jchromb.2005.09.023 |
| format | Article |
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n
=
10). Inter-assay CVs for ADMA-ELISA were 9% for kit control 1 (0.410
±
0.037
μM) and 14% for kit control 2 (1.174
±
0.165
μM). The intra- and inter-assay CVs for HPLC assay for ADMA were 2.5% (0.586
±
0.015
μM) and 4.2% (0.664
±
0.028
μM), respectively. There was no correlation between these two methods (
R
2
=
0.0972). The effect of storage conditions of the samples on ADMA concentrations was investigated by HPLC. ADMA concentration was stable after four freezing and thawing cycles. Overall, the HPLC method offered better sensitivity, selectivity and, very importantly, simultaneous determination of ADMA, SDMA,
l-homoarginine and
l-arginine.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2005.09.023</identifier><identifier>PMID: 16214427</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>ADMA ; Analysis ; Analytical, structural and metabolic biochemistry ; Arginine - analogs & derivatives ; Arginine - blood ; Arginine - chemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Drug Stability ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; HPLC ; Humans ; l-Arginine ; l-Homoarginine ; Medical sciences ; Pharmacology. Drug treatments ; Quality Control ; Reproducibility of Results ; SDMA</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005-12, Vol.828 (1), p.97-102</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-50ea999f42ec777a2ac4bcd1c0356072c09abaa5ae64349d4966551445357dc53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1570023205006641$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17307220$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16214427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valtonen, Pirjo</creatorcontrib><creatorcontrib>Karppi, Jouni</creatorcontrib><creatorcontrib>Nyyssönen, Kristiina</creatorcontrib><creatorcontrib>Valkonen, Veli-Pekka</creatorcontrib><creatorcontrib>Halonen, Toivo</creatorcontrib><creatorcontrib>Punnonen, Kari</creatorcontrib><title>Comparison of HPLC method and commercial ELISA assay for asymmetric dimethylarginine (ADMA) determination in human serum</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>The performance of a new ELISA assay kit (DLD Diagnostika GmbH, Hamburg, Germany) for the determination of asymmetric dimethylarginine (ADMA) was evaluated against a reversed phase HPLC method. ADMA concentrations of 55 serum samples were measured with both methods. The intra-assay CV for ADMA-ELISA was 19% (
n
=
10). Inter-assay CVs for ADMA-ELISA were 9% for kit control 1 (0.410
±
0.037
μM) and 14% for kit control 2 (1.174
±
0.165
μM). The intra- and inter-assay CVs for HPLC assay for ADMA were 2.5% (0.586
±
0.015
μM) and 4.2% (0.664
±
0.028
μM), respectively. There was no correlation between these two methods (
R
2
=
0.0972). The effect of storage conditions of the samples on ADMA concentrations was investigated by HPLC. ADMA concentration was stable after four freezing and thawing cycles. Overall, the HPLC method offered better sensitivity, selectivity and, very importantly, simultaneous determination of ADMA, SDMA,
l-homoarginine and
l-arginine.</description><subject>ADMA</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Arginine - analogs & derivatives</subject><subject>Arginine - blood</subject><subject>Arginine - chemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Drug Stability</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>HPLC</subject><subject>Humans</subject><subject>l-Arginine</subject><subject>l-Homoarginine</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Quality Control</subject><subject>Reproducibility of Results</subject><subject>SDMA</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2P0zAQhiMEYj_gJ4B8AcEhwY7jODmhqizsSkUgARI3a2pPqKvY7toJov9-XTXSHjl5ZD_vjOcpileMVoyy9sO-2utdDG5b1ZSKivYVrfmT4pJ1kpdctr-f5lpIWubr-qK4SmlPKZNU8ufFBWtr1jS1vCz-rYM7QLQpeBIGcvt9syYOp10wBLwhOjiHUVsYyc3m7seKQEpwJEOIuTrmtylaTYw9RY4jxD_WW4_k3erT19V7YnDC6KyHyeb21pPd7MCThHF2L4pnA4wJXy7ndfHr883P9W25-fblbr3alLrpuqkUFKHv-6GpUUspoQbdbLVhmnLRUllr2sMWQAC2DW960_RtK0ReTnAhjRb8unh77nuI4X7GNClnk8ZxBI9hTqrtupZyyjIozqCOIaWIgzpE6yAeFaPqpFzt1aJcnZQr2qvsNudeLwPmrUPzmFocZ-DNAkDSMA4RvLbpkZM871HTzH08c5h1_LUYVdIWvUZjI-pJmWD_85UHULWiDQ</recordid><startdate>20051215</startdate><enddate>20051215</enddate><creator>Valtonen, Pirjo</creator><creator>Karppi, Jouni</creator><creator>Nyyssönen, Kristiina</creator><creator>Valkonen, Veli-Pekka</creator><creator>Halonen, Toivo</creator><creator>Punnonen, Kari</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051215</creationdate><title>Comparison of HPLC method and commercial ELISA assay for asymmetric dimethylarginine (ADMA) determination in human serum</title><author>Valtonen, Pirjo ; Karppi, Jouni ; Nyyssönen, Kristiina ; Valkonen, Veli-Pekka ; Halonen, Toivo ; Punnonen, Kari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-50ea999f42ec777a2ac4bcd1c0356072c09abaa5ae64349d4966551445357dc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>ADMA</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Arginine - analogs & derivatives</topic><topic>Arginine - blood</topic><topic>Arginine - chemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Drug Stability</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>HPLC</topic><topic>Humans</topic><topic>l-Arginine</topic><topic>l-Homoarginine</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Quality Control</topic><topic>Reproducibility of Results</topic><topic>SDMA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valtonen, Pirjo</creatorcontrib><creatorcontrib>Karppi, Jouni</creatorcontrib><creatorcontrib>Nyyssönen, Kristiina</creatorcontrib><creatorcontrib>Valkonen, Veli-Pekka</creatorcontrib><creatorcontrib>Halonen, Toivo</creatorcontrib><creatorcontrib>Punnonen, Kari</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valtonen, Pirjo</au><au>Karppi, Jouni</au><au>Nyyssönen, Kristiina</au><au>Valkonen, Veli-Pekka</au><au>Halonen, Toivo</au><au>Punnonen, Kari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of HPLC method and commercial ELISA assay for asymmetric dimethylarginine (ADMA) determination in human serum</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2005-12-15</date><risdate>2005</risdate><volume>828</volume><issue>1</issue><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>The performance of a new ELISA assay kit (DLD Diagnostika GmbH, Hamburg, Germany) for the determination of asymmetric dimethylarginine (ADMA) was evaluated against a reversed phase HPLC method. ADMA concentrations of 55 serum samples were measured with both methods. The intra-assay CV for ADMA-ELISA was 19% (
n
=
10). Inter-assay CVs for ADMA-ELISA were 9% for kit control 1 (0.410
±
0.037
μM) and 14% for kit control 2 (1.174
±
0.165
μM). The intra- and inter-assay CVs for HPLC assay for ADMA were 2.5% (0.586
±
0.015
μM) and 4.2% (0.664
±
0.028
μM), respectively. There was no correlation between these two methods (
R
2
=
0.0972). The effect of storage conditions of the samples on ADMA concentrations was investigated by HPLC. ADMA concentration was stable after four freezing and thawing cycles. Overall, the HPLC method offered better sensitivity, selectivity and, very importantly, simultaneous determination of ADMA, SDMA,
l-homoarginine and
l-arginine.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16214427</pmid><doi>10.1016/j.jchromb.2005.09.023</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005-12, Vol.828 (1), p.97-102 |
| issn | 1570-0232 1873-376X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ADMA Analysis Analytical, structural and metabolic biochemistry Arginine - analogs & derivatives Arginine - blood Arginine - chemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Drug Stability ELISA Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology General pharmacology HPLC Humans l-Arginine l-Homoarginine Medical sciences Pharmacology. Drug treatments Quality Control Reproducibility of Results SDMA |
| title | Comparison of HPLC method and commercial ELISA assay for asymmetric dimethylarginine (ADMA) determination in human serum |
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