The type III effectors HsvG and HsvB of gall‐forming Pantoea agglomerans determine host specificity and function as transcriptional activators
Summary Pantoea agglomerans pv. gypsophilae (Pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas P. agglomerans pv. betae (Pab) induces galls on both beet and gypsophila. The pathogenicity of both pathovars is dependent on the presence of a plasmid harbouring type III sec...
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| Veröffentlicht in: | Molecular microbiology 2006-09, Vol.61 (5), p.1118-1131 |
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| creator | Nissan, Gal Manulis‐Sasson, Shulamit Weinthal, Dan Mor, Henia Sessa, Guido Barash, Isaac |
| description | Summary
Pantoea agglomerans pv. gypsophilae (Pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas P. agglomerans pv. betae (Pab) induces galls on both beet and gypsophila. The pathogenicity of both pathovars is dependent on the presence of a plasmid harbouring type III secretion system (TTSS) components and effectors. The HsvG TTSS effectors of Pag (HsvG‐Pag) and Pab (HsvG‐Pab) determine the host specificity of both pathovars on gypsophila. Here we describe a novel HsvG homologue, HsvB, which determines the host specificity of Pag and Pab on beet. HsvG requires two direct amino acid repeats for pathogenicity on gypsophila, whereas one repeat in HsvB is sufficient for pathogenicity on beet. Exchanging repeats between HsvG‐Pag and HsvB‐Pab resulted in a switch of host specificities. Transient expression of GFP–HsvG or GFP–HsvB fusions in gypsophila, beet or melon leaves showed that HsvG and HsvB were localized to the nuclei of host and non‐host plants. A yeast one‐hybrid assay revealed that a single repeat of HsvG or HsvB was sufficient to activate transcription. By employing random binding‐site selection and gel‐shift assay HsvG was demonstrated to be a double‐stranded DNA‐binding protein with an ACACC/aAA consensus binding site. These results suggest that HsvG and HsvB are host‐specificity determinants and bear the potential to affect the host transcriptional machinery. |
| doi_str_mv | 10.1111/j.1365-2958.2006.05301.x |
| format | Article |
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Pantoea agglomerans pv. gypsophilae (Pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas P. agglomerans pv. betae (Pab) induces galls on both beet and gypsophila. The pathogenicity of both pathovars is dependent on the presence of a plasmid harbouring type III secretion system (TTSS) components and effectors. The HsvG TTSS effectors of Pag (HsvG‐Pag) and Pab (HsvG‐Pab) determine the host specificity of both pathovars on gypsophila. Here we describe a novel HsvG homologue, HsvB, which determines the host specificity of Pag and Pab on beet. HsvG requires two direct amino acid repeats for pathogenicity on gypsophila, whereas one repeat in HsvB is sufficient for pathogenicity on beet. Exchanging repeats between HsvG‐Pag and HsvB‐Pab resulted in a switch of host specificities. Transient expression of GFP–HsvG or GFP–HsvB fusions in gypsophila, beet or melon leaves showed that HsvG and HsvB were localized to the nuclei of host and non‐host plants. A yeast one‐hybrid assay revealed that a single repeat of HsvG or HsvB was sufficient to activate transcription. By employing random binding‐site selection and gel‐shift assay HsvG was demonstrated to be a double‐stranded DNA‐binding protein with an ACACC/aAA consensus binding site. These results suggest that HsvG and HsvB are host‐specificity determinants and bear the potential to affect the host transcriptional machinery.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2006.05301.x</identifier><identifier>PMID: 16879413</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino acids ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Proteins - physiology ; Base Sequence ; Beta vulgaris - microbiology ; Binding sites ; Caryophyllaceae - microbiology ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - physiology ; Electrophoretic Mobility Shift Assay ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; Green Fluorescent Proteins - physiology ; Microbiology ; Molecular Sequence Data ; Molecular structure ; Pantoea - genetics ; Pantoea - metabolism ; Pantoea - pathogenicity ; Pathogens ; Plasmids - genetics ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Recombinant Fusion Proteins - physiology ; Repetitive Sequences, Amino Acid - genetics ; Species Specificity ; Trans-Activators - genetics ; Trans-Activators - metabolism ; Trans-Activators - physiology ; Transcription, Genetic - genetics ; Two-Hybrid System Techniques ; Virulence - genetics</subject><ispartof>Molecular microbiology, 2006-09, Vol.61 (5), p.1118-1131</ispartof><rights>Copyright Blackwell Publishing Sep 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5101-db4bdc0ad00957f9d58f342b551022d412f161801701f7a413667bd06e34c2cd3</citedby><cites>FETCH-LOGICAL-c5101-db4bdc0ad00957f9d58f342b551022d412f161801701f7a413667bd06e34c2cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.2006.05301.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.2006.05301.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16879413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nissan, Gal</creatorcontrib><creatorcontrib>Manulis‐Sasson, Shulamit</creatorcontrib><creatorcontrib>Weinthal, Dan</creatorcontrib><creatorcontrib>Mor, Henia</creatorcontrib><creatorcontrib>Sessa, Guido</creatorcontrib><creatorcontrib>Barash, Isaac</creatorcontrib><title>The type III effectors HsvG and HsvB of gall‐forming Pantoea agglomerans determine host specificity and function as transcriptional activators</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Pantoea agglomerans pv. gypsophilae (Pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas P. agglomerans pv. betae (Pab) induces galls on both beet and gypsophila. The pathogenicity of both pathovars is dependent on the presence of a plasmid harbouring type III secretion system (TTSS) components and effectors. The HsvG TTSS effectors of Pag (HsvG‐Pag) and Pab (HsvG‐Pab) determine the host specificity of both pathovars on gypsophila. Here we describe a novel HsvG homologue, HsvB, which determines the host specificity of Pag and Pab on beet. HsvG requires two direct amino acid repeats for pathogenicity on gypsophila, whereas one repeat in HsvB is sufficient for pathogenicity on beet. Exchanging repeats between HsvG‐Pag and HsvB‐Pab resulted in a switch of host specificities. Transient expression of GFP–HsvG or GFP–HsvB fusions in gypsophila, beet or melon leaves showed that HsvG and HsvB were localized to the nuclei of host and non‐host plants. A yeast one‐hybrid assay revealed that a single repeat of HsvG or HsvB was sufficient to activate transcription. By employing random binding‐site selection and gel‐shift assay HsvG was demonstrated to be a double‐stranded DNA‐binding protein with an ACACC/aAA consensus binding site. These results suggest that HsvG and HsvB are host‐specificity determinants and bear the potential to affect the host transcriptional machinery.</description><subject>Amino acids</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Proteins - physiology</subject><subject>Base Sequence</subject><subject>Beta vulgaris - microbiology</subject><subject>Binding sites</subject><subject>Caryophyllaceae - microbiology</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Green Fluorescent Proteins - physiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Molecular structure</subject><subject>Pantoea - genetics</subject><subject>Pantoea - metabolism</subject><subject>Pantoea - pathogenicity</subject><subject>Pathogens</subject><subject>Plasmids - genetics</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Recombinant Fusion Proteins - physiology</subject><subject>Repetitive Sequences, Amino Acid - genetics</subject><subject>Species Specificity</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><subject>Trans-Activators - physiology</subject><subject>Transcription, Genetic - genetics</subject><subject>Two-Hybrid System Techniques</subject><subject>Virulence - genetics</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFZDFgl2CfxInWbCACtpIrWBRJHaW45-pR0kcbKft7HiEPiNPgt0ZgcQKb3zl891jXx8AIEYlTuvdrsSU1QXp6rYkCLES1RTh8v4J2PwRnoIN6mpU0JZ8PwEvQtghhCli9Dk4waxtugrTDXi4vtEw7hcN-76H2hgto_MBXoTbcyhmlYuP0Bm4FeP46-eDcX6y8xZ-FXN0WkCx3Y5u0l7MASoddVY1vHEhwrBoaY2VNu4fncw6y2jdDEWAMTdIb5d8IEYoknIr8s0vwTMjxqBfHfdT8O3zp-uzi-Lyy3l_9uGykDVGuFBDNSiJhEJpyMZ0qm4NrchQJ5UQVWFiMMMtwg3CphFpVsaaQSGmaSWJVPQUvD34Lt79WHWIfLJB6nEUs3Zr4KxtWfqvKoFv_gF3bvXp0YHjjtUEVaRNUHuApHcheG344u0k_J5jxHNkfMdzMjwnw3Nk_DEyfp9aXx_912HS6m_jMaMEvD8Ad3bU-_825ldXfa7ob5v3pxg</recordid><startdate>200609</startdate><enddate>200609</enddate><creator>Nissan, Gal</creator><creator>Manulis‐Sasson, Shulamit</creator><creator>Weinthal, Dan</creator><creator>Mor, Henia</creator><creator>Sessa, Guido</creator><creator>Barash, Isaac</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200609</creationdate><title>The type III effectors HsvG and HsvB of gall‐forming Pantoea agglomerans determine host specificity and function as transcriptional activators</title><author>Nissan, Gal ; Manulis‐Sasson, Shulamit ; Weinthal, Dan ; Mor, Henia ; Sessa, Guido ; Barash, Isaac</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5101-db4bdc0ad00957f9d58f342b551022d412f161801701f7a413667bd06e34c2cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino acids</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Proteins - physiology</topic><topic>Base Sequence</topic><topic>Beta vulgaris - microbiology</topic><topic>Binding sites</topic><topic>Caryophyllaceae - microbiology</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Electrophoretic Mobility Shift Assay</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Green Fluorescent Proteins - physiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Molecular structure</topic><topic>Pantoea - genetics</topic><topic>Pantoea - metabolism</topic><topic>Pantoea - pathogenicity</topic><topic>Pathogens</topic><topic>Plasmids - genetics</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Recombinant Fusion Proteins - physiology</topic><topic>Repetitive Sequences, Amino Acid - genetics</topic><topic>Species Specificity</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>Trans-Activators - physiology</topic><topic>Transcription, Genetic - genetics</topic><topic>Two-Hybrid System Techniques</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nissan, Gal</creatorcontrib><creatorcontrib>Manulis‐Sasson, Shulamit</creatorcontrib><creatorcontrib>Weinthal, Dan</creatorcontrib><creatorcontrib>Mor, Henia</creatorcontrib><creatorcontrib>Sessa, Guido</creatorcontrib><creatorcontrib>Barash, Isaac</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nissan, Gal</au><au>Manulis‐Sasson, Shulamit</au><au>Weinthal, Dan</au><au>Mor, Henia</au><au>Sessa, Guido</au><au>Barash, Isaac</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The type III effectors HsvG and HsvB of gall‐forming Pantoea agglomerans determine host specificity and function as transcriptional activators</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2006-09</date><risdate>2006</risdate><volume>61</volume><issue>5</issue><spage>1118</spage><epage>1131</epage><pages>1118-1131</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Pantoea agglomerans pv. gypsophilae (Pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas P. agglomerans pv. betae (Pab) induces galls on both beet and gypsophila. The pathogenicity of both pathovars is dependent on the presence of a plasmid harbouring type III secretion system (TTSS) components and effectors. The HsvG TTSS effectors of Pag (HsvG‐Pag) and Pab (HsvG‐Pab) determine the host specificity of both pathovars on gypsophila. Here we describe a novel HsvG homologue, HsvB, which determines the host specificity of Pag and Pab on beet. HsvG requires two direct amino acid repeats for pathogenicity on gypsophila, whereas one repeat in HsvB is sufficient for pathogenicity on beet. Exchanging repeats between HsvG‐Pag and HsvB‐Pab resulted in a switch of host specificities. Transient expression of GFP–HsvG or GFP–HsvB fusions in gypsophila, beet or melon leaves showed that HsvG and HsvB were localized to the nuclei of host and non‐host plants. A yeast one‐hybrid assay revealed that a single repeat of HsvG or HsvB was sufficient to activate transcription. By employing random binding‐site selection and gel‐shift assay HsvG was demonstrated to be a double‐stranded DNA‐binding protein with an ACACC/aAA consensus binding site. These results suggest that HsvG and HsvB are host‐specificity determinants and bear the potential to affect the host transcriptional machinery.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16879413</pmid><doi>10.1111/j.1365-2958.2006.05301.x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Proteins - physiology Base Sequence Beta vulgaris - microbiology Binding sites Caryophyllaceae - microbiology DNA-Binding Proteins - genetics DNA-Binding Proteins - physiology Electrophoretic Mobility Shift Assay Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Green Fluorescent Proteins - physiology Microbiology Molecular Sequence Data Molecular structure Pantoea - genetics Pantoea - metabolism Pantoea - pathogenicity Pathogens Plasmids - genetics Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombinant Fusion Proteins - physiology Repetitive Sequences, Amino Acid - genetics Species Specificity Trans-Activators - genetics Trans-Activators - metabolism Trans-Activators - physiology Transcription, Genetic - genetics Two-Hybrid System Techniques Virulence - genetics |
| title | The type III effectors HsvG and HsvB of gall‐forming Pantoea agglomerans determine host specificity and function as transcriptional activators |
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