Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ~2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2006-09, Vol.313 (5793), p.1642-1645
Hauptverfasser:	Betzig, Eric, Patterson, George H, Sougrat, Rachid, Lindwasser, O. Wolf, Olenych, Scott, Bonifacino, Juan S, Davidson, Michael W, Lippincott-Schwartz, Jennifer, Hess, Harald F
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins Actins - analysis Algorithms Animals Biological and medical sciences Cell Line Cell Membrane - chemistry Cell membranes Cellular biology Cercopithecus aethiops COS Cells Diverse techniques Fluorescence Focal Adhesions - chemistry Fundamental and applied biological sciences. Psychology Gene Products, gag - analysis HIV-1 Imaging Light Luminescent Proteins - analysis Lysosomes Lysosomes - chemistry Membranes Microscopy Microscopy - methods Mitochondria Mitochondria - chemistry Molecular and cellular biology Molecules Nanotechnology Organelles - chemistry P branes Photobleaching Photons Plasma Proteins Proteins - analysis Pseudopodia - chemistry Recombinant Fusion Proteins - analysis Vinculin - analysis
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