Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells
The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for hum...
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description | The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein. |
doi_str_mv | 10.1007/s00726-005-0221-x |
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J ; Soares-da-Silva, P</creator><creatorcontrib>Fraga, S ; Pinho, M. J ; Soares-da-Silva, P</creatorcontrib><description>The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.</description><identifier>ISSN: 0939-4451</identifier><identifier>EISSN: 1438-2199</identifier><identifier>DOI: 10.1007/s00726-005-0221-x</identifier><identifier>PMID: 16027961</identifier><language>eng</language><publisher>Austria: Springer-Verlag</publisher><subject>Abundance ; Amino Acid Transport System y+ - biosynthesis ; Amino Acid Transport System y+ - genetics ; amino acid transporters ; Amino acids ; Amplification ; Animals ; Antibodies ; Caco-2 Cells ; Cell Line ; Colon ; complementary DNA ; Digestive system ; digestive tract ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Fusion Regulatory Protein 1, Light Chains - biosynthesis ; Fusion Regulatory Protein 1, Light Chains - genetics ; Gene Expression Profiling ; Human ; human cell lines ; Humans ; ileum ; immunoblotting ; intestinal mucosa ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; jejunum ; Large intestine ; Large Neutral Amino Acid-Transporter 1 - biosynthesis ; Large Neutral Amino Acid-Transporter 1 - genetics ; Male ; Mice ; mucosa ; polymerase chain reaction ; Proteins ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA - genetics ; Rodents ; Small intestine</subject><ispartof>Amino acids, 2005-11, Vol.29 (3), p.229-233</ispartof><rights>Springer-Verlag/Wien 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-48eee177db1ebe041a78e7bf36c57ce6a3bd8a39e7b22b66a5bf3181978c97fe3</citedby><cites>FETCH-LOGICAL-c450t-48eee177db1ebe041a78e7bf36c57ce6a3bd8a39e7b22b66a5bf3181978c97fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16027961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fraga, S</creatorcontrib><creatorcontrib>Pinho, M. J</creatorcontrib><creatorcontrib>Soares-da-Silva, P</creatorcontrib><title>Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells</title><title>Amino acids</title><addtitle>Amino Acids</addtitle><description>The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.</description><subject>Abundance</subject><subject>Amino Acid Transport System y+ - biosynthesis</subject><subject>Amino Acid Transport System y+ - genetics</subject><subject>amino acid transporters</subject><subject>Amino acids</subject><subject>Amplification</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Caco-2 Cells</subject><subject>Cell Line</subject><subject>Colon</subject><subject>complementary DNA</subject><subject>Digestive system</subject><subject>digestive tract</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - metabolism</subject><subject>Fusion Regulatory Protein 1, Light Chains - biosynthesis</subject><subject>Fusion Regulatory Protein 1, Light Chains - genetics</subject><subject>Gene Expression Profiling</subject><subject>Human</subject><subject>human cell lines</subject><subject>Humans</subject><subject>ileum</subject><subject>immunoblotting</subject><subject>intestinal mucosa</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>jejunum</subject><subject>Large intestine</subject><subject>Large Neutral Amino Acid-Transporter 1 - biosynthesis</subject><subject>Large Neutral Amino Acid-Transporter 1 - genetics</subject><subject>Male</subject><subject>Mice</subject><subject>mucosa</subject><subject>polymerase chain reaction</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA - genetics</subject><subject>Rodents</subject><subject>Small intestine</subject><issn>0939-4451</issn><issn>1438-2199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1r3DAQQEVoSbZpf0AujaBQenGika2vYwhJW1jIIclZle1xomBbrmTD9t9H7i4UcuhFGmbeDDM8Qs6AXQBj6jLlh8uCMVEwzqHYHZENVKUuOBjzjmyYKU1RVQJOyIeUXhgDrkEekxOQjCsjYUN-3eymiCn5MNLQ0e3VA1A3tmvAqRv8GKhrfEvn6MY0hThjTNSP9HkZ3PiXjG7OiRnT7EfXU5z8_Iy9z2GDfZ8-kved6xN-Ovyn5PH25uH6R7G9-_7z-mpbNJVgc1FpRASl2hqwRlaBUxpV3ZWyEapB6cq61a40Ocd5LaUTuQYajNKNUR2Wp-Trfu4Uw-8lb2MHn9YN3IhhSVZqLTQok8Fv_wWBgZFKVkJk9Msb9CUsMV-5UkZIXXJWZQr2VBNDShE7O0U_uPgnQ3b1ZPeebPZkV092l3s-HyYv9YDtv46DmAyc74HOBeueok_28Z6zXF8tMm7KVwPPlmw</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Fraga, S</creator><creator>Pinho, M. J</creator><creator>Soares-da-Silva, P</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20051101</creationdate><title>Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells</title><author>Fraga, S ; Pinho, M. J ; Soares-da-Silva, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-48eee177db1ebe041a78e7bf36c57ce6a3bd8a39e7b22b66a5bf3181978c97fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Abundance</topic><topic>Amino Acid Transport System y+ - biosynthesis</topic><topic>Amino Acid Transport System y+ - genetics</topic><topic>amino acid transporters</topic><topic>Amino acids</topic><topic>Amplification</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Caco-2 Cells</topic><topic>Cell Line</topic><topic>Colon</topic><topic>complementary DNA</topic><topic>Digestive system</topic><topic>digestive tract</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - metabolism</topic><topic>Fusion Regulatory Protein 1, Light Chains - biosynthesis</topic><topic>Fusion Regulatory Protein 1, Light Chains - genetics</topic><topic>Gene Expression Profiling</topic><topic>Human</topic><topic>human cell lines</topic><topic>Humans</topic><topic>ileum</topic><topic>immunoblotting</topic><topic>intestinal mucosa</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>jejunum</topic><topic>Large intestine</topic><topic>Large Neutral Amino Acid-Transporter 1 - biosynthesis</topic><topic>Large Neutral Amino Acid-Transporter 1 - genetics</topic><topic>Male</topic><topic>Mice</topic><topic>mucosa</topic><topic>polymerase chain reaction</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA - genetics</topic><topic>Rodents</topic><topic>Small intestine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fraga, S</creatorcontrib><creatorcontrib>Pinho, M. J</creatorcontrib><creatorcontrib>Soares-da-Silva, P</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Amino acids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fraga, S</au><au>Pinho, M. J</au><au>Soares-da-Silva, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells</atitle><jtitle>Amino acids</jtitle><addtitle>Amino Acids</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>29</volume><issue>3</issue><spage>229</spage><epage>233</epage><pages>229-233</pages><issn>0939-4451</issn><eissn>1438-2199</eissn><abstract>The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.</abstract><cop>Austria</cop><pub>Springer-Verlag</pub><pmid>16027961</pmid><doi>10.1007/s00726-005-0221-x</doi><tpages>5</tpages></addata></record> |
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subjects | Abundance Amino Acid Transport System y+ - biosynthesis Amino Acid Transport System y+ - genetics amino acid transporters Amino acids Amplification Animals Antibodies Caco-2 Cells Cell Line Colon complementary DNA Digestive system digestive tract Epithelial Cells - cytology Epithelial Cells - metabolism Fusion Regulatory Protein 1, Light Chains - biosynthesis Fusion Regulatory Protein 1, Light Chains - genetics Gene Expression Profiling Human human cell lines Humans ileum immunoblotting intestinal mucosa Intestinal Mucosa - cytology Intestinal Mucosa - metabolism jejunum Large intestine Large Neutral Amino Acid-Transporter 1 - biosynthesis Large Neutral Amino Acid-Transporter 1 - genetics Male Mice mucosa polymerase chain reaction Proteins Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction - methods RNA - genetics Rodents Small intestine |
title | Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells |
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