Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells

The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for hum...

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Veröffentlicht in:Amino acids 2005-11, Vol.29 (3), p.229-233
Hauptverfasser: Fraga, S, Pinho, M. J, Soares-da-Silva, P
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Pinho, M. J
Soares-da-Silva, P
description The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.
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J ; Soares-da-Silva, P</creator><creatorcontrib>Fraga, S ; Pinho, M. J ; Soares-da-Silva, P</creatorcontrib><description>The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.</description><identifier>ISSN: 0939-4451</identifier><identifier>EISSN: 1438-2199</identifier><identifier>DOI: 10.1007/s00726-005-0221-x</identifier><identifier>PMID: 16027961</identifier><language>eng</language><publisher>Austria: Springer-Verlag</publisher><subject>Abundance ; Amino Acid Transport System y+ - biosynthesis ; Amino Acid Transport System y+ - genetics ; amino acid transporters ; Amino acids ; Amplification ; Animals ; Antibodies ; Caco-2 Cells ; Cell Line ; Colon ; complementary DNA ; Digestive system ; digestive tract ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Fusion Regulatory Protein 1, Light Chains - biosynthesis ; Fusion Regulatory Protein 1, Light Chains - genetics ; Gene Expression Profiling ; Human ; human cell lines ; Humans ; ileum ; immunoblotting ; intestinal mucosa ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; jejunum ; Large intestine ; Large Neutral Amino Acid-Transporter 1 - biosynthesis ; Large Neutral Amino Acid-Transporter 1 - genetics ; Male ; Mice ; mucosa ; polymerase chain reaction ; Proteins ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA - genetics ; Rodents ; Small intestine</subject><ispartof>Amino acids, 2005-11, Vol.29 (3), p.229-233</ispartof><rights>Springer-Verlag/Wien 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-48eee177db1ebe041a78e7bf36c57ce6a3bd8a39e7b22b66a5bf3181978c97fe3</citedby><cites>FETCH-LOGICAL-c450t-48eee177db1ebe041a78e7bf36c57ce6a3bd8a39e7b22b66a5bf3181978c97fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16027961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fraga, S</creatorcontrib><creatorcontrib>Pinho, M. J</creatorcontrib><creatorcontrib>Soares-da-Silva, P</creatorcontrib><title>Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells</title><title>Amino acids</title><addtitle>Amino Acids</addtitle><description>The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.</description><subject>Abundance</subject><subject>Amino Acid Transport System y+ - biosynthesis</subject><subject>Amino Acid Transport System y+ - genetics</subject><subject>amino acid transporters</subject><subject>Amino acids</subject><subject>Amplification</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Caco-2 Cells</subject><subject>Cell Line</subject><subject>Colon</subject><subject>complementary DNA</subject><subject>Digestive system</subject><subject>digestive tract</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - metabolism</subject><subject>Fusion Regulatory Protein 1, Light Chains - biosynthesis</subject><subject>Fusion Regulatory Protein 1, Light Chains - genetics</subject><subject>Gene Expression Profiling</subject><subject>Human</subject><subject>human cell lines</subject><subject>Humans</subject><subject>ileum</subject><subject>immunoblotting</subject><subject>intestinal mucosa</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>jejunum</subject><subject>Large intestine</subject><subject>Large Neutral Amino Acid-Transporter 1 - biosynthesis</subject><subject>Large Neutral Amino Acid-Transporter 1 - genetics</subject><subject>Male</subject><subject>Mice</subject><subject>mucosa</subject><subject>polymerase chain reaction</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA - genetics</subject><subject>Rodents</subject><subject>Small intestine</subject><issn>0939-4451</issn><issn>1438-2199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1r3DAQQEVoSbZpf0AujaBQenGika2vYwhJW1jIIclZle1xomBbrmTD9t9H7i4UcuhFGmbeDDM8Qs6AXQBj6jLlh8uCMVEwzqHYHZENVKUuOBjzjmyYKU1RVQJOyIeUXhgDrkEekxOQjCsjYUN-3eymiCn5MNLQ0e3VA1A3tmvAqRv8GKhrfEvn6MY0hThjTNSP9HkZ3PiXjG7OiRnT7EfXU5z8_Iy9z2GDfZ8-kved6xN-Ovyn5PH25uH6R7G9-_7z-mpbNJVgc1FpRASl2hqwRlaBUxpV3ZWyEapB6cq61a40Ocd5LaUTuQYajNKNUR2Wp-Trfu4Uw-8lb2MHn9YN3IhhSVZqLTQok8Fv_wWBgZFKVkJk9Msb9CUsMV-5UkZIXXJWZQr2VBNDShE7O0U_uPgnQ3b1ZPeebPZkV092l3s-HyYv9YDtv46DmAyc74HOBeueok_28Z6zXF8tMm7KVwPPlmw</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Fraga, S</creator><creator>Pinho, M. 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J</au><au>Soares-da-Silva, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells</atitle><jtitle>Amino acids</jtitle><addtitle>Amino Acids</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>29</volume><issue>3</issue><spage>229</spage><epage>233</epage><pages>229-233</pages><issn>0939-4451</issn><eissn>1438-2199</eissn><abstract>The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.</abstract><cop>Austria</cop><pub>Springer-Verlag</pub><pmid>16027961</pmid><doi>10.1007/s00726-005-0221-x</doi><tpages>5</tpages></addata></record>
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subjects Abundance
Amino Acid Transport System y+ - biosynthesis
Amino Acid Transport System y+ - genetics
amino acid transporters
Amino acids
Amplification
Animals
Antibodies
Caco-2 Cells
Cell Line
Colon
complementary DNA
Digestive system
digestive tract
Epithelial Cells - cytology
Epithelial Cells - metabolism
Fusion Regulatory Protein 1, Light Chains - biosynthesis
Fusion Regulatory Protein 1, Light Chains - genetics
Gene Expression Profiling
Human
human cell lines
Humans
ileum
immunoblotting
intestinal mucosa
Intestinal Mucosa - cytology
Intestinal Mucosa - metabolism
jejunum
Large intestine
Large Neutral Amino Acid-Transporter 1 - biosynthesis
Large Neutral Amino Acid-Transporter 1 - genetics
Male
Mice
mucosa
polymerase chain reaction
Proteins
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA - genetics
Rodents
Small intestine
title Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells
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