UVB-Induced G2 Arrest of Human Melanocytes Involves Cdc2 Sequestration by Gadd45a in Nuclear Speckles
Exposure to solar UVB radiation is involved in the development of cutaneous melanoma. We previously showed that human melanocytes and melanoma cells respond to UVB radiation via a p53-independent pathway involving GADD45A activation. Here, we determined that UVB-induction of Gadd45a is necessary for...
Gespeichert in:
| Veröffentlicht in: | Cell cycle (Georgetown, Tex.) Tex.), 2006-08, Vol.5 (16), p.1859-1864 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1864 |
|---|---|
| container_issue | 16 |
| container_start_page | 1859 |
| container_title | Cell cycle (Georgetown, Tex.) |
| container_volume | 5 |
| creator | Fayolle, Caroline Pourchet, Julie Cohen, Aurélie Pedeux, Rémy Puisieux, Alain Fromentel, Claude Caron de Dore, Jean-François Voeltzel, Thibault |
| description | Exposure to solar UVB radiation is involved in the development of cutaneous melanoma. We previously showed that human melanocytes and melanoma cells respond to UVB radiation via a p53-independent pathway involving GADD45A activation. Here, we determined that UVB-induction of Gadd45a is necessary for G2 arrest and that Gadd45a and its partner p21Waf1 co-localize in nuclear bodies called Nuclear Speckles. We further observed that UVB-induced G2 arrest is associated with Cdc2 accumulation in these Nuclear Speckles. Knock-down of Gadd45a expression by RNA interference prevents both UVB-induced Cdc2 accumulation in Nuclear Speckles and G2 arrest. Our results demonstrate that UVB-induced G2 arrest of melanoma cells is Gadd45a-dependent. Furthermore, we show that Cdc2 sequestration by Gadd45a occurs in Nuclear Speckles, suggesting a new role for these nuclear bodies, so far only linked to RNA maturation. |
| doi_str_mv | 10.4161/cc.5.16.3119 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_proquest_miscellaneous_68852094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68852094</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3679-a21fbbb939a80f5865d4b7d24a4db25bf0727d18a899bbcc154c9d527f2c9d7f3</originalsourceid><addsrcrecordid>eNptkUuP0zAQgCMEYpeFG2fkEyfS9Th2Yh9LBW2lAodluVp-RRgSu9hJUf89Li27F04zh2--eVXVa8ALCi3cGrNgC2gXDYB4Ul0DY1BTjNnTU97wmgKGq-pFzj8wJrwT8Ly6glY0IDC_rtz9t_f1NtjZOIvWBC1TcnlCsUebeVQBfXKDCtEcJ5fRNhzicCjJyhqC7tyvuaBJTT4GpI9oraylTCEf0OfZDE4ldLd35ufg8svqWa-G7F5d4k11__HD19Wm3n1Zb1fLXW2athO1ItBrrUUjFMc94y2zVHeWUEWtJkz3uCOdBa64EFobA4waYRnpelJi1zc31buz97sa5D75UaWjjMrLzXInfcgujRJj6Bhn9AAFf3vG9yn-XUaOPhs3lJVdnLNsOWcEC_roNSnmnFz_IAcsT1-QxkgmoZWnLxT8zcU769HZR_hy9gLcnoHSybqsfczGu2DcA1p8Kk2-nPGfsjtX-NDHNKrfMQ1WTuo4xNQnFYzPBfzfMH8AmM6lfw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68852094</pqid></control><display><type>article</type><title>UVB-Induced G2 Arrest of Human Melanocytes Involves Cdc2 Sequestration by Gadd45a in Nuclear Speckles</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Fayolle, Caroline ; Pourchet, Julie ; Cohen, Aurélie ; Pedeux, Rémy ; Puisieux, Alain ; Fromentel, Claude Caron de ; Dore, Jean-François ; Voeltzel, Thibault</creator><creatorcontrib>Fayolle, Caroline ; Pourchet, Julie ; Cohen, Aurélie ; Pedeux, Rémy ; Puisieux, Alain ; Fromentel, Claude Caron de ; Dore, Jean-François ; Voeltzel, Thibault</creatorcontrib><description>Exposure to solar UVB radiation is involved in the development of cutaneous melanoma. We previously showed that human melanocytes and melanoma cells respond to UVB radiation via a p53-independent pathway involving GADD45A activation. Here, we determined that UVB-induction of Gadd45a is necessary for G2 arrest and that Gadd45a and its partner p21Waf1 co-localize in nuclear bodies called Nuclear Speckles. We further observed that UVB-induced G2 arrest is associated with Cdc2 accumulation in these Nuclear Speckles. Knock-down of Gadd45a expression by RNA interference prevents both UVB-induced Cdc2 accumulation in Nuclear Speckles and G2 arrest. Our results demonstrate that UVB-induced G2 arrest of melanoma cells is Gadd45a-dependent. Furthermore, we show that Cdc2 sequestration by Gadd45a occurs in Nuclear Speckles, suggesting a new role for these nuclear bodies, so far only linked to RNA maturation.</description><identifier>ISSN: 1538-4101</identifier><identifier>EISSN: 1551-4005</identifier><identifier>DOI: 10.4161/cc.5.16.3119</identifier><identifier>PMID: 16931908</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>Binding ; Biology ; Bioscience ; Calcium ; Cancer ; CDC2 Protein Kinase ; CDC2 Protein Kinase - metabolism ; Cell ; Cell Cycle ; Cell Cycle Proteins ; Cell Cycle Proteins - metabolism ; Cell Line, Tumor ; Cell Nucleus ; Cell Nucleus - enzymology ; Cell Proliferation ; Cycle ; Cyclin-Dependent Kinase Inhibitor p21 ; Cyclin-Dependent Kinase Inhibitor p21 - metabolism ; G2 Phase ; Humans ; Landes ; Life Sciences ; Melanocytes ; Melanocytes - enzymology ; Melanocytes - radiation effects ; Nuclear Proteins ; Nuclear Proteins - metabolism ; Organogenesis ; Proteins ; Ultraviolet Rays</subject><ispartof>Cell cycle (Georgetown, Tex.), 2006-08, Vol.5 (16), p.1859-1864</ispartof><rights>Copyright © 2006 Landes Bioscience 2006</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3679-a21fbbb939a80f5865d4b7d24a4db25bf0727d18a899bbcc154c9d527f2c9d7f3</citedby><orcidid>0000-0001-7566-3958 ; 0000-0002-9938-3798</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16931908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-00175854$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Fayolle, Caroline</creatorcontrib><creatorcontrib>Pourchet, Julie</creatorcontrib><creatorcontrib>Cohen, Aurélie</creatorcontrib><creatorcontrib>Pedeux, Rémy</creatorcontrib><creatorcontrib>Puisieux, Alain</creatorcontrib><creatorcontrib>Fromentel, Claude Caron de</creatorcontrib><creatorcontrib>Dore, Jean-François</creatorcontrib><creatorcontrib>Voeltzel, Thibault</creatorcontrib><title>UVB-Induced G2 Arrest of Human Melanocytes Involves Cdc2 Sequestration by Gadd45a in Nuclear Speckles</title><title>Cell cycle (Georgetown, Tex.)</title><addtitle>Cell Cycle</addtitle><description>Exposure to solar UVB radiation is involved in the development of cutaneous melanoma. We previously showed that human melanocytes and melanoma cells respond to UVB radiation via a p53-independent pathway involving GADD45A activation. Here, we determined that UVB-induction of Gadd45a is necessary for G2 arrest and that Gadd45a and its partner p21Waf1 co-localize in nuclear bodies called Nuclear Speckles. We further observed that UVB-induced G2 arrest is associated with Cdc2 accumulation in these Nuclear Speckles. Knock-down of Gadd45a expression by RNA interference prevents both UVB-induced Cdc2 accumulation in Nuclear Speckles and G2 arrest. Our results demonstrate that UVB-induced G2 arrest of melanoma cells is Gadd45a-dependent. Furthermore, we show that Cdc2 sequestration by Gadd45a occurs in Nuclear Speckles, suggesting a new role for these nuclear bodies, so far only linked to RNA maturation.</description><subject>Binding</subject><subject>Biology</subject><subject>Bioscience</subject><subject>Calcium</subject><subject>Cancer</subject><subject>CDC2 Protein Kinase</subject><subject>CDC2 Protein Kinase - metabolism</subject><subject>Cell</subject><subject>Cell Cycle</subject><subject>Cell Cycle Proteins</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Nucleus</subject><subject>Cell Nucleus - enzymology</subject><subject>Cell Proliferation</subject><subject>Cycle</subject><subject>Cyclin-Dependent Kinase Inhibitor p21</subject><subject>Cyclin-Dependent Kinase Inhibitor p21 - metabolism</subject><subject>G2 Phase</subject><subject>Humans</subject><subject>Landes</subject><subject>Life Sciences</subject><subject>Melanocytes</subject><subject>Melanocytes - enzymology</subject><subject>Melanocytes - radiation effects</subject><subject>Nuclear Proteins</subject><subject>Nuclear Proteins - metabolism</subject><subject>Organogenesis</subject><subject>Proteins</subject><subject>Ultraviolet Rays</subject><issn>1538-4101</issn><issn>1551-4005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUuP0zAQgCMEYpeFG2fkEyfS9Th2Yh9LBW2lAodluVp-RRgSu9hJUf89Li27F04zh2--eVXVa8ALCi3cGrNgC2gXDYB4Ul0DY1BTjNnTU97wmgKGq-pFzj8wJrwT8Ly6glY0IDC_rtz9t_f1NtjZOIvWBC1TcnlCsUebeVQBfXKDCtEcJ5fRNhzicCjJyhqC7tyvuaBJTT4GpI9oraylTCEf0OfZDE4ldLd35ufg8svqWa-G7F5d4k11__HD19Wm3n1Zb1fLXW2athO1ItBrrUUjFMc94y2zVHeWUEWtJkz3uCOdBa64EFobA4waYRnpelJi1zc31buz97sa5D75UaWjjMrLzXInfcgujRJj6Bhn9AAFf3vG9yn-XUaOPhs3lJVdnLNsOWcEC_roNSnmnFz_IAcsT1-QxkgmoZWnLxT8zcU769HZR_hy9gLcnoHSybqsfczGu2DcA1p8Kk2-nPGfsjtX-NDHNKrfMQ1WTuo4xNQnFYzPBfzfMH8AmM6lfw</recordid><startdate>20060815</startdate><enddate>20060815</enddate><creator>Fayolle, Caroline</creator><creator>Pourchet, Julie</creator><creator>Cohen, Aurélie</creator><creator>Pedeux, Rémy</creator><creator>Puisieux, Alain</creator><creator>Fromentel, Claude Caron de</creator><creator>Dore, Jean-François</creator><creator>Voeltzel, Thibault</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-7566-3958</orcidid><orcidid>https://orcid.org/0000-0002-9938-3798</orcidid></search><sort><creationdate>20060815</creationdate><title>UVB-Induced G2 Arrest of Human Melanocytes Involves Cdc2 Sequestration by Gadd45a in Nuclear Speckles</title><author>Fayolle, Caroline ; Pourchet, Julie ; Cohen, Aurélie ; Pedeux, Rémy ; Puisieux, Alain ; Fromentel, Claude Caron de ; Dore, Jean-François ; Voeltzel, Thibault</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3679-a21fbbb939a80f5865d4b7d24a4db25bf0727d18a899bbcc154c9d527f2c9d7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Binding</topic><topic>Biology</topic><topic>Bioscience</topic><topic>Calcium</topic><topic>Cancer</topic><topic>CDC2 Protein Kinase</topic><topic>CDC2 Protein Kinase - metabolism</topic><topic>Cell</topic><topic>Cell Cycle</topic><topic>Cell Cycle Proteins</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Nucleus</topic><topic>Cell Nucleus - enzymology</topic><topic>Cell Proliferation</topic><topic>Cycle</topic><topic>Cyclin-Dependent Kinase Inhibitor p21</topic><topic>Cyclin-Dependent Kinase Inhibitor p21 - metabolism</topic><topic>G2 Phase</topic><topic>Humans</topic><topic>Landes</topic><topic>Life Sciences</topic><topic>Melanocytes</topic><topic>Melanocytes - enzymology</topic><topic>Melanocytes - radiation effects</topic><topic>Nuclear Proteins</topic><topic>Nuclear Proteins - metabolism</topic><topic>Organogenesis</topic><topic>Proteins</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fayolle, Caroline</creatorcontrib><creatorcontrib>Pourchet, Julie</creatorcontrib><creatorcontrib>Cohen, Aurélie</creatorcontrib><creatorcontrib>Pedeux, Rémy</creatorcontrib><creatorcontrib>Puisieux, Alain</creatorcontrib><creatorcontrib>Fromentel, Claude Caron de</creatorcontrib><creatorcontrib>Dore, Jean-François</creatorcontrib><creatorcontrib>Voeltzel, Thibault</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Cell cycle (Georgetown, Tex.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fayolle, Caroline</au><au>Pourchet, Julie</au><au>Cohen, Aurélie</au><au>Pedeux, Rémy</au><au>Puisieux, Alain</au><au>Fromentel, Claude Caron de</au><au>Dore, Jean-François</au><au>Voeltzel, Thibault</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UVB-Induced G2 Arrest of Human Melanocytes Involves Cdc2 Sequestration by Gadd45a in Nuclear Speckles</atitle><jtitle>Cell cycle (Georgetown, Tex.)</jtitle><addtitle>Cell Cycle</addtitle><date>2006-08-15</date><risdate>2006</risdate><volume>5</volume><issue>16</issue><spage>1859</spage><epage>1864</epage><pages>1859-1864</pages><issn>1538-4101</issn><eissn>1551-4005</eissn><abstract>Exposure to solar UVB radiation is involved in the development of cutaneous melanoma. We previously showed that human melanocytes and melanoma cells respond to UVB radiation via a p53-independent pathway involving GADD45A activation. Here, we determined that UVB-induction of Gadd45a is necessary for G2 arrest and that Gadd45a and its partner p21Waf1 co-localize in nuclear bodies called Nuclear Speckles. We further observed that UVB-induced G2 arrest is associated with Cdc2 accumulation in these Nuclear Speckles. Knock-down of Gadd45a expression by RNA interference prevents both UVB-induced Cdc2 accumulation in Nuclear Speckles and G2 arrest. Our results demonstrate that UVB-induced G2 arrest of melanoma cells is Gadd45a-dependent. Furthermore, we show that Cdc2 sequestration by Gadd45a occurs in Nuclear Speckles, suggesting a new role for these nuclear bodies, so far only linked to RNA maturation.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>16931908</pmid><doi>10.4161/cc.5.16.3119</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-7566-3958</orcidid><orcidid>https://orcid.org/0000-0002-9938-3798</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1538-4101 |
| ispartof | Cell cycle (Georgetown, Tex.), 2006-08, Vol.5 (16), p.1859-1864 |
| issn | 1538-4101 1551-4005 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68852094 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Binding Biology Bioscience Calcium Cancer CDC2 Protein Kinase CDC2 Protein Kinase - metabolism Cell Cell Cycle Cell Cycle Proteins Cell Cycle Proteins - metabolism Cell Line, Tumor Cell Nucleus Cell Nucleus - enzymology Cell Proliferation Cycle Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p21 - metabolism G2 Phase Humans Landes Life Sciences Melanocytes Melanocytes - enzymology Melanocytes - radiation effects Nuclear Proteins Nuclear Proteins - metabolism Organogenesis Proteins Ultraviolet Rays |
| title | UVB-Induced G2 Arrest of Human Melanocytes Involves Cdc2 Sequestration by Gadd45a in Nuclear Speckles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T21%3A38%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=UVB-Induced%20G2%20Arrest%20of%20Human%20Melanocytes%20Involves%20Cdc2%20Sequestration%20by%20Gadd45a%20in%20Nuclear%20Speckles&rft.jtitle=Cell%20cycle%20(Georgetown,%20Tex.)&rft.au=Fayolle,%20Caroline&rft.date=2006-08-15&rft.volume=5&rft.issue=16&rft.spage=1859&rft.epage=1864&rft.pages=1859-1864&rft.issn=1538-4101&rft.eissn=1551-4005&rft_id=info:doi/10.4161/cc.5.16.3119&rft_dat=%3Cproquest_hal_p%3E68852094%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68852094&rft_id=info:pmid/16931908&rfr_iscdi=true |