Epitope Extraction Technique Using a Proteolytic Magnetic Reactor Combined with Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry as a Tool for the Screening of Potential Vaccine Lead Peptides

Epitope extraction technique is based on the specific digestion of a target protein followed by immunoaffinity isolation of a specific recognition peptide. This technique, in combination with mass spectrometry, has been efficiently used for epitope identification. The major goal of this work was to...

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Veröffentlicht in:	European journal of mass spectrometry (Chichester, England) England), 2005-01, Vol.11 (5), p.489-495
Hauptverfasser:	Bílková, Z., Stefanescu, R., Cecal, R., Korecká, L., Ouzká, Š., Ježová, J., Viovy, J.-L., Przybylski, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cyclotrons Enzymes, Immobilized Epitopes - isolation & purification Fourier Analysis Ions Magnetics Mass Spectrometry - methods Peptides - immunology Peptides - isolation & purification Trypsin Vaccines - chemistry
Online-Zugang:	Volltext
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