Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat
Fructans play important roles not only as a carbon source for survival under persistent snow cover but also as agents that protect against various stresses in overwintering plants. Complex fructans having both beta-(2,1)- and beta-(2,6)-linked fructosyl units accumulate in wheat (Triticum aestivum L...
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| Veröffentlicht in: | Planta 2005-12, Vol.223 (1), p.90-104 |
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| description | Fructans play important roles not only as a carbon source for survival under persistent snow cover but also as agents that protect against various stresses in overwintering plants. Complex fructans having both beta-(2,1)- and beta-(2,6)-linked fructosyl units accumulate in wheat (Triticum aestivum L.) during cold hardening. We detected fructan: fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100) activity for catalyzing the formation and extension of beta-(2,1)-linked fructans in hardened wheat tissues, cloned cDNAs (wft3 and wft4) of 1-FFT, and analyzed the enzymatic properties of a wft3 recombinant protein (Wft3m) produced by yeast. Wft3m transferred beta-(2,1)-linked fructosyl units to phlein, an extension of sucrose through beta-(2,6)-linked fructosyl units, as well as to inulin, an extension of sucrose through beta-(2,1)-linked fructosyl units, but could not efficiently synthesize long inulin oligomers. Incubation of a mixture of Wft3m and another recombinant protein of wheat, sucrose:fructan 6-fructosyltransferase (6-SFT), with sucrose and 1-kestotriose produced fructans similar to those that accumulated in hardened wheat tissues. The results demonstrate that 1-FFT produces branches of beta-(2,1)-linked fructosyl units to phlein and graminan oligomers synthesized by 6-SFT and contributes to accumulation of fructans containing beta-(2,1)- and beta-(2,6)-linked fructosyl units. In combination with sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and 6-SFT, 1-FFT is necessary for fructan synthesis in hardened wheat. |
| doi_str_mv | 10.1007/s00425-005-0054-6 |
| format | Article |
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Complex fructans having both beta-(2,1)- and beta-(2,6)-linked fructosyl units accumulate in wheat (Triticum aestivum L.) during cold hardening. We detected fructan: fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100) activity for catalyzing the formation and extension of beta-(2,1)-linked fructans in hardened wheat tissues, cloned cDNAs (wft3 and wft4) of 1-FFT, and analyzed the enzymatic properties of a wft3 recombinant protein (Wft3m) produced by yeast. Wft3m transferred beta-(2,1)-linked fructosyl units to phlein, an extension of sucrose through beta-(2,6)-linked fructosyl units, as well as to inulin, an extension of sucrose through beta-(2,1)-linked fructosyl units, but could not efficiently synthesize long inulin oligomers. Incubation of a mixture of Wft3m and another recombinant protein of wheat, sucrose:fructan 6-fructosyltransferase (6-SFT), with sucrose and 1-kestotriose produced fructans similar to those that accumulated in hardened wheat tissues. The results demonstrate that 1-FFT produces branches of beta-(2,1)-linked fructosyl units to phlein and graminan oligomers synthesized by 6-SFT and contributes to accumulation of fructans containing beta-(2,1)- and beta-(2,6)-linked fructosyl units. In combination with sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and 6-SFT, 1-FFT is necessary for fructan synthesis in hardened wheat.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-005-0054-6</identifier><identifier>PMID: 16034592</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Biological and medical sciences ; Biosynthesis ; Carbohydrate Conformation ; Carbohydrate Metabolism ; Carbon sources ; Chromatography, Ion Exchange ; Cloning, Molecular ; Cold Climate ; Cold hardening ; cold stress ; Complementary DNA ; enzyme activity ; Enzymes ; Flowering ; fructan-fructan fructosyltransferase ; Fructans ; Fructans - biosynthesis ; Fructans - chemistry ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; Gene Library ; graminan ; hexosyltransferases ; Hexosyltransferases - chemistry ; Hexosyltransferases - metabolism ; messenger RNA ; Metabolism ; Molecular Sequence Data ; Oligomers ; Overwintering ; Pichia - genetics ; Plant physiology and development ; plant response ; plant stress ; Plants ; recombinant fusion proteins ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Recombinant proteins ; RNA, Messenger - metabolism ; Seasons ; Sequence Alignment ; Snow cover ; Solubility ; Sucrose ; Transfection ; Triticum - chemistry ; Triticum - enzymology ; Triticum - genetics ; Triticum aestivum ; Wheat ; winter wheat ; Yeasts</subject><ispartof>Planta, 2005-12, Vol.223 (1), p.90-104</ispartof><rights>Springer-Verlag Berlin Heidelberg 2005</rights><rights>2006 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-a9cbd3f41157426f14de57aaae4ad3298b1a86d70b445b0e7e804e045cfea5ed3</citedby><cites>FETCH-LOGICAL-c468t-a9cbd3f41157426f14de57aaae4ad3298b1a86d70b445b0e7e804e045cfea5ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23389113$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23389113$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17491840$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16034592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawakami, A</creatorcontrib><creatorcontrib>Yoshida, M</creatorcontrib><title>Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat</title><title>Planta</title><addtitle>Planta</addtitle><description>Fructans play important roles not only as a carbon source for survival under persistent snow cover but also as agents that protect against various stresses in overwintering plants. Complex fructans having both beta-(2,1)- and beta-(2,6)-linked fructosyl units accumulate in wheat (Triticum aestivum L.) during cold hardening. We detected fructan: fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100) activity for catalyzing the formation and extension of beta-(2,1)-linked fructans in hardened wheat tissues, cloned cDNAs (wft3 and wft4) of 1-FFT, and analyzed the enzymatic properties of a wft3 recombinant protein (Wft3m) produced by yeast. Wft3m transferred beta-(2,1)-linked fructosyl units to phlein, an extension of sucrose through beta-(2,6)-linked fructosyl units, as well as to inulin, an extension of sucrose through beta-(2,1)-linked fructosyl units, but could not efficiently synthesize long inulin oligomers. Incubation of a mixture of Wft3m and another recombinant protein of wheat, sucrose:fructan 6-fructosyltransferase (6-SFT), with sucrose and 1-kestotriose produced fructans similar to those that accumulated in hardened wheat tissues. The results demonstrate that 1-FFT produces branches of beta-(2,1)-linked fructosyl units to phlein and graminan oligomers synthesized by 6-SFT and contributes to accumulation of fructans containing beta-(2,1)- and beta-(2,6)-linked fructosyl units. In combination with sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and 6-SFT, 1-FFT is necessary for fructan synthesis in hardened wheat.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Metabolism</subject><subject>Carbon sources</subject><subject>Chromatography, Ion Exchange</subject><subject>Cloning, Molecular</subject><subject>Cold Climate</subject><subject>Cold hardening</subject><subject>cold stress</subject><subject>Complementary DNA</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>Flowering</subject><subject>fructan-fructan fructosyltransferase</subject><subject>Fructans</subject><subject>Fructans - biosynthesis</subject><subject>Fructans - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>Gene Library</subject><subject>graminan</subject><subject>hexosyltransferases</subject><subject>Hexosyltransferases - chemistry</subject><subject>Hexosyltransferases - metabolism</subject><subject>messenger RNA</subject><subject>Metabolism</subject><subject>Molecular Sequence Data</subject><subject>Oligomers</subject><subject>Overwintering</subject><subject>Pichia - genetics</subject><subject>Plant physiology and development</subject><subject>plant response</subject><subject>plant stress</subject><subject>Plants</subject><subject>recombinant fusion proteins</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Recombinant proteins</subject><subject>RNA, Messenger - metabolism</subject><subject>Seasons</subject><subject>Sequence Alignment</subject><subject>Snow cover</subject><subject>Solubility</subject><subject>Sucrose</subject><subject>Transfection</subject><subject>Triticum - chemistry</subject><subject>Triticum - enzymology</subject><subject>Triticum - genetics</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><subject>winter wheat</subject><subject>Yeasts</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkdGL1DAQxoMo3nr6B_igBkGfrE6apEl9k8NT4cAHvecwbSe7XdvmLmmR9a83e1088GGYge83H8M3jD0X8F4AmA8JQJW6ALgrVVQP2EYoWRYlKPuQbQDyDLXUZ-xJSnuALBrzmJ2JCqTSdblh42Vc2hmnj37tXBR3U0iHYY44JU8RE73jyH_RgdP05zAS9yHyps_MNO8o9YkHz7cRx37KDmHot2GkmHg_8R3Gjibq-O8d4fyUPfI4JHp26ufs-vLzz4uvxdX3L98uPl0VrarsXGDdNp30SghtVFl5oTrSBhFJYSfL2jYCbdUZaJTSDZAhC4pA6dYTaurkOXu7-t7EcLtQmt3Yp5aGAScKS3KVtfqYWAZf_wfuwxKnfJuzJdhsa2SGxAq1MaQUybub2I8YD06AOz7CrY9w2fJYylV55-XJeGlG6u43Tsln4M0JwNTi4HPWbZ_uOaNqYRVk7sXK7dMc4j-9lNLWQhyPe7XqHoPDbcwe1z9KEBIEGC2hkn8BaAmkjw</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Kawakami, A</creator><creator>Yoshida, M</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat</title><author>Kawakami, A ; Yoshida, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-a9cbd3f41157426f14de57aaae4ad3298b1a86d70b445b0e7e804e045cfea5ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Metabolism</topic><topic>Carbon sources</topic><topic>Chromatography, Ion Exchange</topic><topic>Cloning, Molecular</topic><topic>Cold Climate</topic><topic>Cold hardening</topic><topic>cold stress</topic><topic>Complementary DNA</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>Flowering</topic><topic>fructan-fructan fructosyltransferase</topic><topic>Fructans</topic><topic>Fructans - biosynthesis</topic><topic>Fructans - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>Gene Library</topic><topic>graminan</topic><topic>hexosyltransferases</topic><topic>Hexosyltransferases - chemistry</topic><topic>Hexosyltransferases - metabolism</topic><topic>messenger RNA</topic><topic>Metabolism</topic><topic>Molecular Sequence Data</topic><topic>Oligomers</topic><topic>Overwintering</topic><topic>Pichia - genetics</topic><topic>Plant physiology and development</topic><topic>plant response</topic><topic>plant stress</topic><topic>Plants</topic><topic>recombinant fusion proteins</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Recombinant proteins</topic><topic>RNA, Messenger - metabolism</topic><topic>Seasons</topic><topic>Sequence Alignment</topic><topic>Snow cover</topic><topic>Solubility</topic><topic>Sucrose</topic><topic>Transfection</topic><topic>Triticum - chemistry</topic><topic>Triticum - enzymology</topic><topic>Triticum - genetics</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><topic>winter wheat</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawakami, A</creatorcontrib><creatorcontrib>Yoshida, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawakami, A</au><au>Yoshida, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>223</volume><issue>1</issue><spage>90</spage><epage>104</epage><pages>90-104</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Fructans play important roles not only as a carbon source for survival under persistent snow cover but also as agents that protect against various stresses in overwintering plants. Complex fructans having both beta-(2,1)- and beta-(2,6)-linked fructosyl units accumulate in wheat (Triticum aestivum L.) during cold hardening. We detected fructan: fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100) activity for catalyzing the formation and extension of beta-(2,1)-linked fructans in hardened wheat tissues, cloned cDNAs (wft3 and wft4) of 1-FFT, and analyzed the enzymatic properties of a wft3 recombinant protein (Wft3m) produced by yeast. Wft3m transferred beta-(2,1)-linked fructosyl units to phlein, an extension of sucrose through beta-(2,6)-linked fructosyl units, as well as to inulin, an extension of sucrose through beta-(2,1)-linked fructosyl units, but could not efficiently synthesize long inulin oligomers. Incubation of a mixture of Wft3m and another recombinant protein of wheat, sucrose:fructan 6-fructosyltransferase (6-SFT), with sucrose and 1-kestotriose produced fructans similar to those that accumulated in hardened wheat tissues. The results demonstrate that 1-FFT produces branches of beta-(2,1)-linked fructosyl units to phlein and graminan oligomers synthesized by 6-SFT and contributes to accumulation of fructans containing beta-(2,1)- and beta-(2,6)-linked fructosyl units. In combination with sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and 6-SFT, 1-FFT is necessary for fructan synthesis in hardened wheat.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>16034592</pmid><doi>10.1007/s00425-005-0054-6</doi><tpages>15</tpages></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Biological and medical sciences Biosynthesis Carbohydrate Conformation Carbohydrate Metabolism Carbon sources Chromatography, Ion Exchange Cloning, Molecular Cold Climate Cold hardening cold stress Complementary DNA enzyme activity Enzymes Flowering fructan-fructan fructosyltransferase Fructans Fructans - biosynthesis Fructans - chemistry Fundamental and applied biological sciences. Psychology gene expression regulation Gene Library graminan hexosyltransferases Hexosyltransferases - chemistry Hexosyltransferases - metabolism messenger RNA Metabolism Molecular Sequence Data Oligomers Overwintering Pichia - genetics Plant physiology and development plant response plant stress Plants recombinant fusion proteins Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombinant proteins RNA, Messenger - metabolism Seasons Sequence Alignment Snow cover Solubility Sucrose Transfection Triticum - chemistry Triticum - enzymology Triticum - genetics Triticum aestivum Wheat winter wheat Yeasts |
| title | Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat |
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