Genotyping of thiopurine methyltransferase in patients with acute leukemia using LightCycler PCR
Patients with acute lymphoblastic leukemia (ALL) are treated with thiopurines. Thiopurine methyltransferase (TPMT) catalyses the S-methylation of thiopurines and is subject to genetic polymorphism. The TPMT genotype was determined in 55 patients with ALL. Three patients were heterozygous for allelic...
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| Veröffentlicht in: | Leukemia & lymphoma 2006-08, Vol.47 (8), p.1624-1628 |
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| container_title | Leukemia & lymphoma |
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| creator | Davison, Julie E. A. McMullin, Mary Frances Catherwood, Mark A. |
| description | Patients with acute lymphoblastic leukemia (ALL) are treated with thiopurines. Thiopurine methyltransferase (TPMT) catalyses the S-methylation of thiopurines and is subject to genetic polymorphism. The TPMT genotype was determined in 55 patients with ALL. Three patients were heterozygous for allelic variants of TPMT. We describe an assay for the genotyping of the TPMT polymorphism using real-time fluorescence polymerase chain reaction (PCR) to facilitate rapid processing of samples. This strategy is superior to standard PCR-restriction fragment length polymorphism genotyping methods and provides informative data on TPMT polymorphisms in patients prior to treatment with thiopurines. |
| doi_str_mv | 10.1080/10428190500518800 |
| format | Article |
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This strategy is superior to standard PCR-restriction fragment length polymorphism genotyping methods and provides informative data on TPMT polymorphisms in patients prior to treatment with thiopurines.</description><subject>Acute Disease</subject><subject>acute leukemia</subject><subject>Case-Control Studies</subject><subject>Exons</subject><subject>Fluorescence</subject><subject>Genotype</subject><subject>genotyping</subject><subject>Humans</subject><subject>Leukemia - enzymology</subject><subject>Leukemia - genetics</subject><subject>LightCycler</subject><subject>Methyltransferases - genetics</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - standards</subject><subject>Polymorphism, Genetic</subject><subject>Temperature</subject><subject>TPMT</subject><issn>1042-8194</issn><issn>1029-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEGL1TAURosozjj6A9xIVu6qN2lem6IbeYyj8EARXcckvZlmTJOapAz99_bxHogIs8pdnO8QTlW9pPCGgoC3FDgTtIcdwI4KAfCouqTA-ppxaB4fb87qDeAX1bOc72DD-pY9rS5o27ct69rL6ucNhljW2YVbEi0po4vzklxAMmEZV1-SCtliUhmJC2RWxWEomdy7MhJlloLE4_ILJ6fIko-Wg7sdy341HhP5uv_2vHpilc_44vxeVT8-Xn_ff6oPX24-7z8casOBl5p31AjOhe5RdB2nVg_UAjBkHQ6aCeBGNZ3VDEE1VPfMDJ0ebNtrpq3CXXNVvT555xR_L5iLnFw26L0KGJcsWyG4gE5sID2BJsWcE1o5JzeptEoK8phV_pd127w6yxc94fB3ce64Ae9PgAs2pkndx-QHWdTqY7JbQuOybB7yv_tnPqLyZTQqobyLSwpbuAd-9wfev5mp</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Davison, Julie E. 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A.</creatorcontrib><creatorcontrib>McMullin, Mary Frances</creatorcontrib><creatorcontrib>Catherwood, Mark A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia & lymphoma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davison, Julie E. A.</au><au>McMullin, Mary Frances</au><au>Catherwood, Mark A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotyping of thiopurine methyltransferase in patients with acute leukemia using LightCycler PCR</atitle><jtitle>Leukemia & lymphoma</jtitle><addtitle>Leuk Lymphoma</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>47</volume><issue>8</issue><spage>1624</spage><epage>1628</epage><pages>1624-1628</pages><issn>1042-8194</issn><eissn>1029-2403</eissn><abstract>Patients with acute lymphoblastic leukemia (ALL) are treated with thiopurines. Thiopurine methyltransferase (TPMT) catalyses the S-methylation of thiopurines and is subject to genetic polymorphism. The TPMT genotype was determined in 55 patients with ALL. Three patients were heterozygous for allelic variants of TPMT. We describe an assay for the genotyping of the TPMT polymorphism using real-time fluorescence polymerase chain reaction (PCR) to facilitate rapid processing of samples. This strategy is superior to standard PCR-restriction fragment length polymorphism genotyping methods and provides informative data on TPMT polymorphisms in patients prior to treatment with thiopurines.</abstract><cop>United States</cop><pub>Informa UK Ltd</pub><pmid>16966276</pmid><doi>10.1080/10428190500518800</doi><tpages>5</tpages></addata></record> |
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| ispartof | Leukemia & lymphoma, 2006-08, Vol.47 (8), p.1624-1628 |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Acute Disease acute leukemia Case-Control Studies Exons Fluorescence Genotype genotyping Humans Leukemia - enzymology Leukemia - genetics LightCycler Methyltransferases - genetics Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Polymorphism, Genetic Temperature TPMT |
| title | Genotyping of thiopurine methyltransferase in patients with acute leukemia using LightCycler PCR |
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